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At least 19 recordsLinked to original sources
PubMed2004

Vertebrate primary cilia: a sensory part of centrosomal complex in tissue cells, but a "sleeping beauty" in cultured cells?

Primary cilium development along with other components of the centrosome in mammalian cells was analysed ultrastructurally and by immunofluorescent staining with anti-acetylated tubulin antibodies. We categorized two types of primary cilia, nascent cilia that are about 1microm long located inside the cytoplasm, and true primary cilia that are several microm long and protrude from the plasma membrane. The primary cilium is invariably associated with the older centriole of each diplosome, having appendages at the distal end and pericentriolar satellites with cytoplasmic microtubules emanating from them. Only one cilium per cell is formed normally through G(0), S and G(2)phases. However, in some mouse embryo fibroblasts with two mature centrioles, bicilates were seen. Primary cilia were not observed in cultured cells where the mature centriole had no satellites and appendages (Chinese hamster kidney cells, line 237, some clones of l-fibroblasts). In contrast to primary cilia, striated rootlets were found around active and non-active centrioles with the same frequency. In proliferating cultured cells, a primary cilium can be formed several hours after mitosis, in fibroblasts 2-4 h after cell division and in PK cells only during the S-phase. In interphase cells, formation of the primary cilium can be stimulated by the action of metabolic inhibitors and by reversed depolymerization of cytoplasmic microtubules with cold or colcemid treatments. In mouse renal epithelial cells in situ, the centrosome was located near the cell surface and mature centrioles in 80% of the cells had primary cilium protruding into the duct lumen. After cells were explanted and subcultured, the centrosome comes closer to the nucleus and the primary cilium was depolymerized or reduced. Later primary cilia appeared in cells that form islets on the coverslip. However, the centrosome in cultured ciliated cells was always located near the cell nucleus and primary cilium never formed a characteristic distal bulb. A sequence of the developmental stages of the primary cilium is proposed and discussed. We also conclude that functioning primary cilium does not necessarily operate in culture cells, which might explain some of the contradictory data on cell ciliation in vitro reported in the literature.

Animals↗
PubMed2002

A qualitative study of teenagers' decisions to undergo orthodontic treatment with fixed appliance.

OBJECTIVE: The aim of this study was to describe thoughts and values influencing young people's choices to undergo orthodontic treatment. SUBJECTS AND METHODS: Twenty-eight patients (11 boys), aged 13-19 years, at an orthodontic clinic in the western part of Sweden participated. Open, taped interviews, lasting about 1 hour, were conducted with each subject and analysed by the grounded theory method. Five descriptive categories, each related to several subcategories, were generated in the analysis and labelled: 'being like everyone else', 'being diagnosed', 'focusing on the mouth', 'obeying social norms' and 'forced decision-making'. OUTCOME: Category forced decision-making was identified as a core category, describing the power in the social process, resulting in the decision to undergo orthodontic treatment. CONCLUSIONS: Motivation for the decision to undergo orthodontic treatment seemed to be social norms, and the beauty culture in their reference group and in society in general. The teenagers were not fully conscious of these external influences. Their opinion, as a group, was that they had made an independent decision to undergo orthodontic treatment.

Adolescent↗
PubMed1998

Age and gender differences in body attitudes: a comparison of young and elderly adults.

One hundred and thirty-two young adults (Mean = 19 years) and 142 elderly adults (Mean = 74 years) evaluated thirty-five different aspects of their own bodies. As hypothesized, elderly adults expressed less positive attitudes than young adults toward body items associated with body functioning (physical coordination, agility, sex drive, health). These differences are consistent with research indicating a progressive decline in bodily function efficiency with advancing age (Christofalo, 1988; Lakatta, 1990). Also as expected, the elderly held less positive attitudes toward body aspects associated with facial attractiveness (lips, appearance of eyes, cheek/cheekbones). These differences are in line with the structural changes that occur in the face as people age, moving them further from cultural beauty standards. One area where these age differences were reversed was in women's attitudes toward weight-related body items: elderly women expressed greater satisfaction than young women toward their appetite, thighs, and weight. The cause of this age difference in women may be due to thinness being a more defining standard of attractiveness for young women, or it could be due to the fact that people typically lose weight after the age of fifty, thus making weight gain less of a concern for older women. Results further indicated that, although men have more positive body attitudes than women, this gender difference is not nearly as pronounced among the elderly.

Adult↗
PubMed2004

Counterselection and co-delivery of transposon and transposase functions for Sleeping Beauty-mediated transposition in cultured mammalian cells.

Sleeping Beauty (SB) is a gene-insertion system reconstructed from transposon sequences found in teleost fish and is capable of mediating the transposition of DNA sequences from transfected plasmids into the chromosomes of vertebrate cell populations. The SB system consists of a transposon, made up of a gene of interest flanked by transposon inverted repeats, and a source of transposase. Here we carried out a series of studies to further characterize SB-mediated transposition as a tool for gene transfer to chromosomes and ultimately for human gene therapy. Transfection of mouse 3T3 cells, HeLa cells, and human A549 lung carcinoma cells with a transposon containing the neomycin phosphotransferase (NEO) gene resulted in a several-fold increase in drug-resistant colony formation when co-transfected with a plasmid expressing the SB transposase. A transposon containing a methotrexate-resistant dihydrofolate reductase gene was also found to confer an increased frequency of methotrexate-resistant colony formation when co-transfected with SB transposase-encoding plasmid. A plasmid containing a herpes simplex virus thymidine kinase gene as well as a transposon containing a NEO gene was used for counterselection against random recombinants (NEO+TK+) in medium containing G418 plus ganciclovir. Effective counterselection required a recovery period of 5 days after transfection before shifting into medium containing ganciclovir to allow time for transiently expressed thymidine kinase activity to subside in cells not stably transfected. Southern analysis of clonal isolates indicated a shift from random recombination events toward transposition events when clones were isolated in medium containing ganciclovir as well as G418. We found that including both transposon and transposase functions on the same plasmid substantially increased the stable gene transfer frequency in Huh7 human hepatoma cells. The results from these experiments contribute technical and conceptual insight into the process of transposition in mammalian cells, and into the optimal provision of transposon and transposase functions that may be applicable to gene therapy studies.

3T3 Cells↗
PubMed1979

A method of quantifying cultural ideals of beauty and the obese.

A series of photographs of persons which had been varied by anamorphic lens to produce images both thinner and thicker than the originals were assessed by three experts in terms of percentage deviation from ideal body weight. Together with the photographs, five questions about preferences for body size were put to 341 persons who were also asked to assign each photograph to one of six basic categories of body weight in Puerto Rican culture.

Beauty↗