SEARCH · PubMed Health

Results for “BREEDING”

Explore indexed PubMed citations for clinical trials, systematic reviews and public health research. Read source abstracts and follow each citation to its original PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 19 recordsLinked to original sources

PubMed1991

Across-breed expected progeny differences: use of within-breed expected progeny differences to adjust breed evaluations for sire sampling and genetic trend.

Data on 2,034 F1 calves sired by Angus, Hereford, Polled Hereford, Charolais, Limousin, Simmental, Gelbvieh, and Tarentaise bulls with Hereford or Angus dams and data on 3,686 three-breed-cross calves with 700 F1 dams of the same breed crosses were used for this study. Traits analyzed were birth, weaning, yearling, and 420-d weights (BWT, WW, YW, and W420, respectively) of F1 calves and WW of three-breed-cross calves. Expected progeny differences from national cattle evaluation programs for sires of F1 calves and cows for BWT, WW, YW, and net maternal ability (milk) were used to assess their value in prediction of crossbred performance. Regressions of actual F1 calf performance on sire EPD were positive for BWT (1.09 +/- .12 kg/kg of BWT EPD), WW (.79 +/- .14 kg/kg of WW EPD), YW (1.44 +/- .16 kg/kg of YW EPD), and W420 (1.66 kg/kg of YW EPD). These regression coefficients were similar to the expected value of 1.0 for BWT and WW but were larger than expected for YW and W420. Regressions of actual three-breed-cross calf WW on milk and WW EPD of their maternal grandsires were .95 +/- .14 and .42 +/- .10 kg/kg, respectively, and differed little from their expectations of 1.0 and .5, respectively. Observed breed of sire means for each trait were adjusted for sire sampling by using EPD regressions to adjust them to the average EPD of all sires of each breed born in 1970.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗

PubMed1993

Performance of hair breeds and prolific wool breeds of sheep in southern Illinois: effect of breed of service sire on lamb production of Suffolk and Targhee ewes.

The effects of year, age of ewe, sex of lamb, breed of ewe, and breed of service ram on fertility, prolificacy, lamb survival, lamb weaning weight, and ewe productivity of Suffolk and Targhee ewes mated to Finnsheep, Combo-6, Booroola Merino, St. Croix, and Barbados rams were estimated. Matings were in four consecutive years using three rams per breed of service ram each year, with a total of 758 ewe-year observations. There was a significant effect of year on fertility, prolificacy, and ewe productivity. Two- and three-year-old ewes had lowest (P < .05) fertility rates, whereas 3-, 4-, and 5(+)-yr-old ewes had higher (P < .01) prolificacy and ewe productivity than 2-yr-old ewes. Ram lambs were .9 kg heavier (P < .01) at weaning than were ewe lambs. Suffolk ewes gave birth to .13 more (P < .05) lambs per ewe lambing, and their lambs were 1.4 kg heavier (P < .01) than lambs from Targhee ewes. Ewes mated to St. Croix and Barbados rams had higher (P < .10) fertility rates than ewes mated to Finnsheep rams. Lambs sired by Barbados, Finnsheep, and St. Croix rams had higher (P < .05) survival rates to weaning than lambs sired by Booroola Merino rams. The heaviest (P < .01) lambs at weaning were those sired by Finnsheep and Combo-6 rams. Ewes mated to hair-breed rams had greater (P < .05) performance for the fitness traits of fertility and lamb survival but lower (P < .01) lamb weaning weights than ewes mated to wool-breed rams.(ABSTRACT TRUNCATED AT 250 WORDS)

Age Factors↗

PubMed1995

Heterogeneity of variance by sire breed, sex, and dam breed in 200- and 365-day weights of beef cattle from a top cross experiment.

The nature of the heterogeneity of variance for 200- and 365-d weights by sex, sire breed, and dam breed subclasses was studied. Data consisted of records for weaning (n = 7,829) and yearling (n = 7,367) weights of progeny from 673 and 672 sires, respectively, from 22 breeds that have been evaluated in the Germ Plasm Evaluation Program at the U.S. Meat Animal Research Center, Clay Center, NE. Sires were mated to Hereford and Angus cows. Each trait was analyzed separately. Three studies were undertaken separately to investigate heterogeneity due to the different factors (i.e., sire breed, sex, or dam breed). Only data from seven sire breeds were used to study the factor sire breed, but all data (22 sire breeds) were used to study the factors sex and dam breed. In each study, three sire and dam models with records of animals of the four sex x dam breed combinations considered different traits and with the same model equation, but covariance structures for random effects (sires, dams, and residuals) of increasing generality were fitted. First, (co)variances across subclasses were assumed equal. Second, correlations and fractions of phenotypic variance were assumed equal but phenotypic variance differed by sire breed, sex, or dam breed as appropriate. Third, variances and covariances were different for each subclass of the factor under study. Variance components were estimated by derivative-free REML. Models for each trait and each factor were compared through likelihood ratio tests. For both traits, variances differed (P < .02) in scale, but not as fractions of phenotypic variance (P > .10), by sire breed and sex subclasses. Variances were not different (P > .10) by dam breed subclasses, either in scale or as fractions of phenotypic variance. Estimates of correlations among genetic effects on weights of calves from different sex-dam breed subclasses were at least .85. Across all sex, sire breed, and dam breed subclasses, pooled estimates of sire and dam variances as fractions of phenotypic variance were, respectively, .06 and .39 for weaning weight and .11 and .24 for yearling weight. The conclusion is that the assumption of equal phenotypic variances among sire breeds and between sexes may not be appropriate in genetic evaluations.

Algorithms↗

PubMed2000

Evaluation of Dorset, Finnsheep, Romanov, Texel, and Montadale breeds of sheep: I. Effects of ram breed on productivity of ewes of two crossbred populations.

Effects of Dorset, Finnsheep, Romanov, Texel, and Montadale breeds for performance as sires were estimated in the initial phase of a comprehensive evaluation of these breeds as contributors to sheep crossbreeding systems. Objectives were to evaluate the effects of ram breed, ewe breed, season of mating, and two-way interactions. Rams from the five breeds were single-sire-mated with ewes from two breed types to produce lambs over a 3-yr period. Ewes were assigned to one of three distinct 35-d mating seasons initiated each year in August, October, and December. A different sample of six rams per breed was used each year across all three seasons, and each ram was penned with ewes of both breeds. Traits evaluated and number of ewe records were conception rate and litter weaning weight per ewe exposed (n = 3,261) and number born, litter birth weight, average birth weight, number weaned, and litter weaning weight per ewe lambing (n = 2,751). Ram breed and ewe breed interacted (P < .01) for conception rate and litter weaning weight per ewe exposed, implicating mating preferences, particularly of Romanov rams. In mixed groups of ewes exposed to Romanov rams, conception rate was 12.7% lower and litter weight weaned was 8.4 kg lower in the ewe breed presumably less preferred for mating by the rams. On a per ewe exposed basis, Romanov-sired litters produced either the largest or the smallest values for litter weaning weight, depending on the breed of ewe. Effects of ram breed on number born and litter birth weight interacted (P < .05) with season of mating. The largest litters within each ram breed were associated with the October mating season. Montadale and Romanov rams sired larger and heavier litters from August matings than from December matings, whereas the opposite was true for Dorset-sired litters. Texel- and Finnsheep-sired litters were similar in size and weight from August and December matings. Breed of ram differences affected per ewe lambing productivity measurements (P < .01). Differences between ram breeds for ewe productivity were noted, with increased number born and improved survival of crossbred progeny to weaning for Romanov-sired litters. These results may have implications for using these ram breeds as sires in different crossbreeding systems. Structured mating systems or the creation of new composite populations involving these breeds could be used to match the resources, environment, and market of specific production situations.

Animals↗

PubMed1995

Performance of hair breeds and prolific wool breeds of sheep in southern Illinois: lamb production of F1 adult ewes.

Two- and three-year-old F1 crossbred ewes produced from Suffolk and Targhee dams and sires of the prolific wool breeds of Finnsheep, Combo-6, and Booroola Merino and the hair breeds of St. Croix and Barbados were pasture-mated to Dorset rams over a 4-yr period at the Dixon Springs Agricultural Center in Southern Illinois. Booroola Merino sires were homozygous for the FecB allele for high number of ovulations. The effects of age of ewe, sex of lamb (where appropriate), breed of dam, and breed of sire on condition score, breeding weight, ovulation rate, breeding to lambing interval, fertility, prolificacy, lamb survival, weaning weight, and ewe productivity were estimated. All traits were analyzed using mixed-model methodology. Crossbred ewes from Suffolk dams had a higher (P < .01) condition score, heavier (P < .01) breeding weight, higher (P < .01) ovulation rate, shorter (P < .01) breeding to lambing interval, greater (P < .01) prolificacy, heavier (P < .01) lambs at weaning, and greater (P < .05) ewe productivity than crossbred ewes from Targhee dams. Ewes from hair-breed sires had lighter (P < .01) breeding weight, lower (P < .05) ovulation rate, shorter (P < .05) breeding to lambing interval, higher (P < .05) fertility, higher (P < .05) lamb survival, lower (P < .10) lamb weaning weight, and higher (P < .05) ewe productivity than ewes sired by the prolific wool-breed rams. The breed of sire x breed of dam interaction was a significant source of variation for breeding weight, fertility, lamb survival, and ewe productivity.(ABSTRACT TRUNCATED AT 250 WORDS)

Alleles↗

PubMed1998

Urolithiasis in dogs. II: Breed prevalence, and interrelations of breed, sex, age, and mineral composition.

OBJECTIVE: To analyze selected breed-related data for canine urinary calculi. SAMPLE POPULATION: 11,000 specimens: 5,781 from female dogs, 5,215 from males, and 4 from dogs of unrecorded sex. PROCEDURE: Information was compiled for all canine urinary calculi submitted between July 1981 and January 1994. Results for a mixed-breed group and 26 of the most common breeds of stone-forming dogs were analyzed. Interrelations of breed, sex, and age of affected dogs and mineral composition of the specimens were determined. RESULTS: Prevalence of 5 specific mineral types was significantly correlated between the sexes of 27 common breed groups: struvite, calcium phosphate (apatite), calcium oxalate, brushite, and urate. Struvite-containing calculi were seen in high proportions in both sexes of 7 breeds, and in low proportions in both sexes of 7 other breeds. Male and female Lhasa Apsos, Cairn Terriers, and 5 other breeds had high proportions of oxalate-containing calculi; values in males were substantially higher. Low numbers of oxalate-containing calculi were seen in both sexes of 7 breeds; Dalmatians had the lowest numbers. Males and females of 6 breeds had high numbers of urate-containing calculi, Dalmatians and English Bulldogs had the highest numbers. Low amounts of urate were found in calculi from males and females of 6 breeds, Samoyeds had the lowest numbers. Highest proportions of cystine-containing calculi were seen in male Dachshunds, English Bulldogs, and Chihuahuas. Males of 8 breeds had no specimens that contained cystine; only 2 such specimens were obtained from females. CONCLUSIONS: Prevalence of uroliths differs among breed, age, and sex of affected dogs. CLINICAL RELEVANCE: Breed, sex, and age of dogs; mineral types of calculi in males versus females; and their anatomic location within the tract are important considerations for clinicians when evaluating risk in dogs with urolithiasis and in identifying areas that need further in-depth applied or clinical investigation, or both.

Age Distribution↗

PubMed1993

Breed comparisons for growth traits adjusted for within-breed genetic trend using expected progeny differences.

Records (2,910) of birth (BWT), weaning (WW), and yearling weight (YW) of F1 calves produced in a top-cross experiment involving Angus, Hereford, Pooled Hereford, Charolais, Limousin, Simmental, Gelbvieh, Maine-Anjou, Chianina, Tarentaise, Shorthorn, and Salers bulls mated to Hereford and Angus cows and records (4,592) of WW on three-breed-cross calves out of 986 F1 females of the same breed crosses were used in this study. The purposes were to estimate how much of the EPD of the sires was realized in crossbred calves and to estimate sire breed effects for the traits adjusted for genetic trend and sire sampling. Published EPD for BWT, WW, YW, net maternal ability (MLK), and maternal WW (MAT) were used. Average regressions (kilograms/kilogram +/- SE) of BWT, WW, and YW of F1 calves on EPD of the sire were 1.04 +/- .10, .88 +/- .11, and 1.40 +/- .11, respectively. The regressions (b, kilograms/kilogram) were similar to the expected values of 1.0 except for YW. For WW of three-way-cross calves on MLK EPD of the maternal grandsire, b was 1.02 +/- .11, which was not different from the expected value of 1.0. Estimated sire-breed means were adjusted to a 1982 genetic base by adding b times the difference of the 1982-breed-mean EPD and mean EPD of sires used in the study. Three different adjustments were compared using the b pooled across breeds, a separate b for each breed, and the expected b of 1.0. In general, the adjustments tended to regress breed of sire means toward the average of all breeds, particularly for BWT and WW of F1 calves, and for WW of three-breed crosses. The effect of type of adjustment varied among breeds, but in most cases small differences resulted from using average or expected b. For WW, the range for net maternal effects among breeds was larger than that for direct breed effects.

Animals↗

PubMed1996

Performance of hair breeds and prolific wool breeds of sheep in southern Illinois: wool production and fleece quality.

The objective of this study was to compare weight and quality of fleeces of different F1 ewe types produced from breeds with a broad range of fleece types. Weights of 629 fleeces produced during 1988 through 1991 from F1 ewes that were daughters of Suffolk and Targhee dams and Finnsheep, Combo-6, Booroola Merino, St. Croix, and Barbados sires were recorded. Staple length was measured on the mid-side of each ewe present in 1991. Fleeces shorn in 1991 were sent to a wool marketing organization, and staple length, wool grade, and clean fleece yield were subjectively estimated (n = 220). Mid-side fleece samples were collected from no more than two randomly selected ewes from each subclass (breed of dam-breed of sire-age of ewe) in 1991 (n = 78) and sent to a wool laboratory where fiber diameter, yield, and percentage of colored, med, and kemp fibers were objectively determined. Ewes from Targhee dams produced fleeces with greater weight, greater fiber length, smaller fiber diameter, lower yield, and fewer colored fibers than ewes from Suffolk dams (all differences significant, P < .01). Booroola Merino-sired ewes produced heavier (P < .01) fleeces than did Finnsheep- and Combo-6-sired ewes (4.13 and 3.09 kg, respectively), and in turn, Finnsheep- and Combo-6-sired ewes produced heavier (P < .01) fleeces than did ewes sired by hair breed rams (3.09 and 1.70 kg, respectively). Among hair breed-sired ewes, St. Croixsired ewes produced heavier (P < .01) fleeces than did Barbados-sired ewes (1.88 and 1.52 kg, respectively). Fleeces produced by Booroola Merino-sired ewes had smaller (P < .01) fiber diameter than all sire breed groups except Combo-6-sired ewes, and fleeces produced by St. Croix-sired ewes had greater (P < .01) fiber diameter than all other sire breed groups. Lab scoured yield was greater (P < .01) for fleeces from ewes from hair breed than for fleeces from ewes from wool breed sires (74.2 vs 66.1%). Proportions of undesirable fibers (med, kemp, and colored) were 20 to 600 times greater (P < .01) in fleeces of ewes from hair breed sires than in fleeces of ewes from wool breed sires. In general, F1 ewes from Booroola Merino sires produced the heaviest, highest quality fleeces, and ewes from the hair breed sires of St. Croix and Barbados produced the lightest, lowest quality fleeces. Ewes from Finnsheep and Combo-6 sires produced fleeces that were more similar to the fleeces of ewes from Booroola Merino sires than to the fleeces of ewes from the hair breed sires.

Animals↗

PubMed1999

Genetic trends and breed overlap derived from multiple-breed genetic evaluations of beef cattle for growth traits.

Genetic evaluations for a multiple-breed population of beef cattle were used to estimate genetic trends for five breeds, and genetic differences and overlap among 14 breeds. Genetic evaluations studied were for direct contributions to birth weight, gain from birth to 200 and 365 d, and maternal contribution to gain from birth to 200 d. Almost all genetic trends were positive, but the magnitude of the trends varied among breeds. Trends were nonlinear between 1985 and 1995 for most breed and trait combinations. The rates of increase in genetic trends were generally higher for the lighter weight breeds, and lighter weight breeds had faster growth rate genetic trends at 1995 than the heavier breeds. Genetic trend estimates for yearling gain at 1995 were 2.46, 2.23, 1.73, 1.70, and 1.46 kg/yr for Angus, Hereford, Limousin, Charolais, and Simmental, respectively. Corresponding birth weight genetic trends were .130, .226, .049, .130, and .048 kg/yr. Mean genetic differences between breeds have been decreasing in magnitude due to these differences in genetic trends between heavier and lighter breeds. Genetic variation for the traits studied seemed to be greater within than between breeds for calves born and cows calving between 1993 and 1995. Genetic trends at 1995 suggest that ratios of within:between breed variation will increase and that across-breed genetic improvement initiatives for growth traits will become more important in the future.

Animals↗

PubMed1987

Simulation of genetic control of reproduction in beef cows. III. Within-herd breeding value estimation with known breeding dates.

Procedures for breeding value estimation for reproductive traits with known breeding dates were developed and tested using results of a computer simulation model of genetic control of bovine reproduction. The model generated realized reproductive outputs as a function of underlying genetic variation in two independent traits: conception rate (CR), which was indicative of the ability to conceive given estrus, and PPI, the postpartum interval from calving to first estrus. Two scenarios were considered. In the first, all cows were assumed to be cycling at the start of breeding and to be bred artificially. For this scenario, breeding values for CR could be estimated from information on observed breeding and calving dates by using a categorical trait, multi-stage selection model. Breeding value estimation for PPI, however, required actual measurement of PPI because if PPI and CR are genetically independent and if all cows are cycling at the beginning of breeding, subsequent breeding and calving dates are independent of PPI. The second scenario recognized that not all females would be cycling at the start of breeding. For this scenario, the categorical trait, multi-stage selection model could still be applied for breeding value estimation for CR, but accumulation of data across years was complicated by a need to consider the lifetime reproductive pattern of the individual rather than just the sum of each year's performances. Breeding value estimates for PPI could be obtained from observed breeding and calving dates for this scenario, but required consideration of the distributional properties of PPI.

Animals↗

PubMed1983

Estimation of the interaction of dams with hatches, sires in breed, and breed of sire for five traits in the fowl.

An experiment was designed to estimate the interaction of dams by hatches, dams by sires in the same breed, and dams by breed of sires. White Leghorn dams were mated sequentially to two White Leghorn sires and two New Hampshire sires, and two hatches were measured for each sire. Two replicates were included, each with different sires and dams. In this design, breed of sire is factorial to replicates. Sires in breed are nested in replicates and breeds of sires. Hatches are nested in replicates, breeds of sire, and sires in breeds. Dams are nested in replicates but are factorial to breeds of sires, sires in breeds, and hatches in sires in breeds, so that the interactions of the dams with these variables can be estimated. The traits observed were percent hatch of all eggs set, body weight at 8 weeks of age, age at first egg, percent production from first egg to 36 weeks of age, and egg weight at 32 weeks of age. No significant interactions were observed for any trait between dams and either breed of sire or sires in breed. The only significant interaction observed was that for dam by hatch for body weight at 8 weeks of age.

Animals↗

PubMed1998

[Position of Russian chicken breeds in the diversity of Eurasian breeds].

Eleven old Russian, four Mediterranean, and nine Asian chicken breeds were compared with the wild species Gallus gallus (red jungle fowl) by 31 discrete morphological characters. The majority of the characters studied show monogenic inheritance. Five large clusters can be detected on a dendrogram constructed on the basis of the morphological characters of the studied breeds. Almost all breeds characterized by their a similar practical use (egg, meat/egg, meat, game, and ornamental) were included in the same cluster independently of their geographical origin. Close morphological and genetic relations of Mediterranean breeds to the wild species Gallus gallus was confirmed. A wide typological diversity of old Russian breeds was shown; five types of breeds were found among them. Russian breeds were joined in the same clusters with similar types of breeds of Mediterranean or Asian origin. However, 10 out of 11 studied Russian breeds were found to be more closely related to Asian breeds. Thus, it may be suggested that the gene pool of old Russian was formed largely under the influence of Asian breeds and, to a lower extent, Mediterranean breeds.

Animals↗

PubMed2003

Sex differences in cortisol secretion after administration of an ACTH analogue in sheep during the breeding and non-breeding season.

The aim of this study was to compare the response of cortisol in sheep of different sex and gonadal status to adrenal cortex stimulation by an ACTH analogue in the breeding and non-breeding season. Twenty-four adult Corriedale sheep were used in the non-breeding season, and 19 in the breeding season. Three weeks prior to the first trial (non-breeding season), six rams and six ewes were gonadectomised. In each trial, blood was obtained every 15min for 9h and the animals received 0.5mg of ACTH (Tetracosactid, Synacthen Depot i.m., after 1.5h of sampling. Sampling began at 10:00a.m. in the non-breeding season and at 9:00a.m. in the breeding season. Three main effects (sex, gonadal status and season) were evaluated, each with two levels (male and female, intact and gonadectomised, breeding and non-breeding season, respectively). In both seasons, the females showed higher cortisol levels after ACTH than males (P<0.001), though the difference seemed less marked in the non-breeding season. The cortisol response in the ewes was not affected by season. The rams, however, showed a lower response in the breeding season (P<0.03). Gonadectomy reduced the response in the ewes (P<0.001) but had no effect in the rams. Nevertheless, gonadectomy also eliminated the differences between the ewes and the rams, such that the intact rams had lower levels of cortisol compared to the intact females, with those of the gonadectomised animals of both sexes being intermediate between the gonad-intact groups. The results of this study confirm sex differences in ACTH induced cortisol secretion in intact sheep in vivo. Furthermore, by applying exogenous ACTH we have directly stimulated the adrenal cortex, indicating the existence of sex differences also at this level. The circulating gonadal steroids, which are responsible at least in part for the sex differences in the responses to stress, may influence cortisol secretion from the adrenal gland by direct action at the cortex.

Adrenal Cortex↗

PubMed2004

Genetic comparison of breeding schemes based on semen importation and local breeding schemes: framework and application to Costa Rica.

Local breeding schemes for Holstein cattle of Costa Rica were compared with the current practice based on continuous semen importation (SI) by deterministic simulation. Comparison was made on the basis of genetic response and correlation between breeding goals. A local breeding goal was defined on the basis of prevailing production circumstances and compared against a typical breeding goal for an exporting country. Differences in genetic response were <3%, and the correlation between breeding goals was 0.99. Therefore, difference between breeding objectives proved negligible. For the evaluation of genetic response, the current scheme based on SI was evaluated against a progeny testing (PT) scheme and a closed nucleus (CN) breeding scheme, both local. Selection intensities and accuracy of selection were defined according to current population size and reproduction efficiency parameters. When genotype x environment interaction (G x E) was ignored, SI was the strategy with the highest genetic response: 5.0% above the CN breeding scheme and 33.2% above PT. A correlation between breeding values in both countries lower than one was assumed to assess the effect of G x E. This resulted in permanent effects on the relative efficiencies of breeding strategies because of the reduction in the rate of genetic response when SI was used. When the genetic correlation was assumed equal to 0.75, the genetic response achieved with SI was reduced at the same level as local PT. When an initial difference in average genetic merit of the populations was assumed, this only had a temporal effect on the relative ranking of strategies, which is reverted after some years of selection because the rate of change in genetic responses remains unchanged. Given that the actual levels of genetic correlation between countries may be around 0.60, it was concluded that a local breeding scheme based on a nucleus herd could provide better results than the current strategy based on SI.

Animals↗

PubMed2002

Within-breed heterozygosity of canine single nucleotide polymorphisms identified by across-breed comparison.

Identification of single nucleotide polymorphisms (SNPs) by DNA sequence comparison across breeds is a strategy for developing genetic markers that are useful for many breeds. However, the heterozygosity of SNPs identified in this way might be severely reduced within breeds by inbreeding or genetic drift in the small effective population size of a breed (population subdivision). The effect of inbreeding and population subdivision on heterozygosity of SNPs in dog breeds has never been investigated in a systematic way. We determined the genotypes of dogs from three divergent breeds for SNPs in four canine genes (ACTC, LMNA, SCGB, and TYMS) identified by across-breed DNA sequence comparison, and compared the genotype frequencies to those expected under Hardy-Weinberg equilibrium (HWE). Although population subdivision significantly skewed allele frequencies across breeds for two of the SNPs, the deviations of observed heterozygosities compared with those expected within breeds were minimal. These results indicate that across-breed DNA sequence comparison is a reasonable strategy for identifying SNPs that are useful within many canine breeds.

Animals↗

PubMed2001

Breed demarcation and potential for breed allocation of horses assessed by microsatellite markers.

Population demarcation of eight horse breeds was investigated using genotype information of 306 horses from 26 microsatellite loci. The breeds include the indigenous Norwegian breeds Fjord Horse, Nordland/Lyngen Horse, Døle Horse and Coldblooded Trotter together with Icelandic Horse, Shetland Pony, Standardbred and Thoroughbred. Both phylogenetic analysis and a maximum likelihood method were applied to examine the potential for breed allocation of individual animals. The phylogenetic analysis utilizing simple allele sharing statistics revealed clear demarcation among the breeds; 95% of the individuals clustered together with animals of the same breed in the phylogenetic tree. Even breeds with a short history of divergence like Døle Horse and Coldblooded Trotter formed distinct clusters. Implementing the maximum likelihood method allocated 96% of the individuals to their source population, applying an assignment stringency of a log of the odds ratio larger than 2. Lower allocation stringency assigned nearly all the horses. Only three individuals were wrongly allocated a breed by both methods. In conclusion, the study demonstrates clear distinction among horse breeds, and by combining the two assignment methods breed allocation could be determined for more than 99% of the individuals.

Alleles↗

PubMed1996

Risk of recurrence of repeat breeding in sows with a repeat breeding in the first parity.

Risk of recurrence of repeat breeding in sows with a repeat breeding in the 1st parity was investigated in 241 sow herds (1985-1990). The association between repeat breeding in the 1st and subsequent parities was examined using data on 9,120 sows. Each sow contributed only one pair of observations to the total data set. Occurrence of repeat breeding in the 1st and higher parities was presented in terms of recurrent risk rates (rRR), adjusted for different strata of herds. Sows with a repeat breeding in the 1st parity did not have a significantly higher risk rate for recurrence of a repeat breeding in the 2nd parity (rRR = 1.12, 95% confidence interval .97-1.29) and in the 3rd parity (rRR = 1.10, 95% confidence interval .86-1.41) compared to sows without a repeat breeding in the 1st parity. Sows with a repeat breeding in the 1st parity had a slightly higher risk rate for recurrence of a repeat breeding in the 4th or higher parity (rRR = 1.07, 95% confidence interval 1.03-1.10), compared to sows without a repeat breeding in the 1st parity. However, this risk is, in a biological sense, very small.

Animals↗

PubMed1999

Testicular mitosis, meiosis and apoptosis in mink (Mustela vison) during breeding and non-breeding seasons.

Testes of mink were compared between the breeding (March) and non-breeding seasons with the start (November) and cessation (May) of spermatogenic activity. Testicular mass and spermatozoa per gram testis were assessed. Percentages of haploid (1C), diploid (2C) and tetraploid (4C) cells were monitored using DNA flow cytometry and the proportions of somatic and spermatogenetic cells were determined after selective labelling of somatic cells with a vimentin antibody. Apoptosis was examined by cell death detection ELISA, and testosterone concentrations were measured with an enzyme-immunoassay. The significantly higher testis mass during the breeding period coincided with higher numbers of testicular spermatozoa per gram testis and peak of testicular testosterone concentration in comparison with non-breeding periods. The proportions of 1C, 2C and 4C cells showed corresponding strong differences between these periods with the maximum of 1C cells during breeding. The proportions of testicular cells in G2-M phase of mitosis were very low during the period of peak spermatogenesis; they were markedly increased in the time of autumnal resumption in November but were even higher during testis involution in May. However. the meiotic transformation (1C:4C ratio) is maximal in March. The total as well as the relative proportions of spermatogenic and somatic cells differed significantly not only between breeding and non-breeding periods but also between the periods at the start and at the end of active spermatogenesis. The intensity of apoptosis was also seasonally dependent. The highest level in March indicates a stimulated apoptosis even during the breeding period. In conclusion, the production of spermatozoa in mink is intensified by enlargement of gonads as well as enhanced efficiency of spermatogenesis during breeding. In this time, the testosterone concentration and the meiotic transformation show high levels, but the mitotic activity of spermatogenic cells is already significantly diminished and an intensified apoptosis seems to precede the forthcoming testis involution after breeding. The results suggest that the regulation of seasonal testicular activity is characterised by co-ordinated shifts in the relationships between mitosis, meiosis, apoptosis and testosterone production.

Animals↗