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PubMedDate not supplied

[Correlation of skin test to grass pollen with basophil degranulation test results in 72 patients (author's transl)].

Seventy-two subjects have been tested by skin-test (ST) and human basophil degranulation test (HBDT) with grass pollen extract (GPE). HBDT was done, using a suspension enriched in basophils, by a technic on slide. Results are expressed by an index (DI) which is percentage of basophils having apparently disappeared (i. e. degranulated) in presence of GPE. No less than 100 intact basophils (and often many more) were always counted in absence of GPE (control) for each subject, so that DI higher than 35% are significant (p less than 0,001). DI were higher than 35% in 28 positive ST subjects for at less one of three dilutions of GPE (10(-4), 10(-6), 10(-8)); in the 5 subjects with false-negative result, 3 had a weak ST response. In 39 subjects with negative ST, DI were higher than 35% in two subjects and only for one dilution (10(-4)). So, concordance of results of HBDT and obviously positive ST reaches 94%. In 33 positive ST subjects, a significant correlation appears between DI (at 10(-4) and 10(-6)) and flare diameter and between DI (at 10(-4)) and wheals diameter. HBDT appears to be a suitable and simple technic for routinely study of reaginic allergies.

Basophils↗
PubMed1990

[The use of the direct basophil degranulation test for the diagnosis of allergy].

Basophil degranulation test (BDT) was used in examinations of patients suffering from allergies. A modified technique was employed with toluidine blue staining and basophil scintillation in a counter chamber. The examinees were divided into three groups. Group 1 (n = 34) consisted of patients suffering from atopic bronchial asthma with suspected sensitization to home dust. Group 2 included 118 patients with a history of allergy to penicillin. Group 3 consisted of 51 patients with drug allergies (streptomycin, vitamin B1, procaine, analgin intolerance). In all the cases specific diagnosis was confirmed by skin and provocation test. 71.4 percent of the patients with sensitization to home dust allergen showed positive results in BDT, as did 68.6 percent of Group 3 subjects. Of the cases with penicillin sensitization confirmed by application test 91.7 percent were positive in BDT, of those with positive intradermal tests, 66.7 percent were BDT-positive, this being probably explained by individual sensitivity of the patients. These results recommend BDT, an informative and simple technique, for wide practice.

Asthma↗
PubMed1979

[Immunological investigations of house-dust and house-dust mites. Part III. A simplified basophil degranulation test. Preliminary communication (author's transl)].

The basophil degranulation test (BDT) after Shelley has been simplified and tried on patients allergic to house-dust or to house-dust mites. All 20 patients reacted to the mite extracts and 19 out of 20 to the house dust extracts. Out of 20 control persons 17 had negative results to the mite extract and 16 to the house-dust extract. The advantages of this new simplified method are discussed.

Basophils↗
PubMed1987

[Basophil degranulation test in suspected mould allergy].

Mould allergy is often linked with a pattern of signs and symptoms of the upper airways, especially of the nose. Since exact aetiopathological correlations are hard to determine by anamnesis only, further allergy diagnosis is very important. This often yields marked differences between intracutaneous and nasal provocation tests. The human basophil degranulation test (HBDT) now offers a further method of in-vitro diagnosis. This test can explore the condition of the basophils, which are of great importance for the allergic reaction besides the mast cells, and the ability of these cells to degranulate in the presence of a corresponding allergen. We investigated the correlations between the results of HBDT, intracutaneous skin test and nasal provocation test. 30 patients with perennial rhinitis suspected of being caused by moulds were tested using 4 mould mixtures. We found many more positive results with the nasal test and HBDT than with the skin test. The corrected contingency coefficient after Pearson showed a stronger correlation of HBDT to the incracutaneous skin test and to the nasal provocation test than between the two in-vivo methods. This is thought to be due to a wider sensitivity spectrum of the degranulation test which can possibly also measure other allergy reactions than type I after Commbs and Gell. We consider HBDT to be a valuable additional tool in allergy diagnosis. We should welcome a wider range of different allergenic test slides and a standardisation of allergenic extracts.

Adolescent↗
PubMed1975

The direct basophil degranulation test and the intracutaneous test: a comparison using food extracts.

The purpose of this study was to compare the direct basophil degranulation test (DBDT) of Hirsch and Zastrow with the intracutaneous (IC) test in subjects with and without histories of food allergy. The DBDT was perfomred by incubating the concentrated leukocytes of subjects with dilutions of the suspected allergenic extract and comparing the number of nondegranulated basophils remaining in the test samples with the number remaining in a control. The test sample with the least number of basophils represents maximal basophil degranulation. The DBDT was perfromed with extracts of the suspected food on 14 subjects with reliable clinical histories and positive IC tests indicative of food hypersensitivity. Thirteen of these 14 food-sensitive subjects also had IC and DDBT8 to the extracts of foods to which they were not clinically sensitive. Fourteen different subjects not allergic to foods were also tested with food extracts by the IC and DBDTS. There was a 92% correlation between the IC test and the DBDT, a 90% correlation between the IC test and the history, and an 86% correlation between the DBDT and the history. We conclude that, in this study of subjects clearly sensitive to foods, the DBDT correlates favorably with the history and IC test.

Allergens↗
PubMed1979

[The basophil degranulation test in the diagnosis of bee and wasp sting allergies].

Comparison of basophil degranulation test (BDT) with radioallergosorbent test (RAST) in 50 patients with anamnestically certain or probable hymenoptera allergy yielded practically equal results. In patients who had experienced their allergic reaction a long time ago, BDT seemed to react positively over a longer period of time than RAST. As BDT is technically easy to perform and moderate in price, it is suited to substitute RAST.

Adolescent↗
PubMed1987

[Value of the basophil degranulation test (BDT) in the follow-up of immunotherapy with hymenoptera venoms].

The basophil degranulation test (BDT) with the plasma of 24 patients undergoing venom immunotherapy was usually negative after only 6 months of treatment. Cellular sensitivity, however, could still be proved by BDT on purified cells, i.e. after removal of the plasma which contained blocking IgG antibodies. In 30% of the cases, we found primary desensitization of the basophils, which means that the IgG antibodies did not have any influence on the test results.

Basophils↗
PubMedDate not supplied

Basophil degranulation test in house dust mite allergy: diagnostic value.

After reviewing the experience of various authors we applied the Basophil Degranulation Test (BDT) in the study of sensitization to Dermatophagoides pteronyssinus, so as to evaluate its diagnostic use. Fifty-five patients with asthma and/or rhinitis and a positive skin test to the mentioned antigen, as well as 20 control subjects were studied. The results demonstrated a sensitivity of 65.5% and a specificity of 80% with an efficiency index of 69.5%. We did not find any significant correlation with the histamine release test (r = 0.236). The data obtained and the proper limitations of the technique led us to consider the BDT to have a limited application in routine allergy diagnosis.

Adolescent↗
PubMed1987

[Comparative study of the human basophil degranulation test and total and specific IgE levels in schistosomiasis. 54 cases].

In 54 patients (45 Africans and 9 West-Indians) we searched schistosomiasis and the presence of immunoglobulins E by different tests (total immunoglobulin E levels, measurement of specific IgE, human basophil degranulation test). In patients with an excretion of living eggs we observed 91.6% of positive responses for the II. B. D. T. and 100% for the specific IgE. In cases only identified by examination of rectal mucosa biopsy specimens showing apparently living eggs, we observed 75% and 87.5% of positive responses for the same tests. But in both categories of patients, the parasitological examination is the most important. Moreover the authors observed "false-positive" and "false negative" tests compared with the parasitological results and between the tests. It suggests that the best use of these is in case of very probable schistosomiasis without positive parasitological results.

Africa↗
PubMed1987

[Basophil degranulation test (BDT) as a parameter of hyposensitization with Hymenoptera venoms].

In a population of 24 insect sting allergy patients undergoing venom immunotherapy the basophil degranulation test (BDT) in the patient sera ("unwashed" BDT) and with washed leukocytes ("washed" BDT) after incubation with bee and wasp (yellow jacket) venom was performed before and during treatment. Venom specific IgE and IgG antibodies, detected by means of RAST, were also monitored. The "unwashed" BDT usually became negative within 6-9 months of beginning immunotherapy, whereas the IgE-RAST was still clearly positive. This was attributed to the blocking influence of the venom specific IgG antibodies induced by the venom therapy. In fact, at this time the BDT with "washed" blood leukocytes, i.e. after elimination of the serum antibodies, was generally still positive. Only during further immunotherapy did cellular sensitization in the "washed" BDT gradually disappear, whereas the IgE-RAST usually turned out weakly positive. A third of the patients showed simultaneously negative results of "unwashed" and "washed" BDT, independently of venom specific IgE and IgG levels. These findings suggest a specific reactivity change of the blood basophils (cellular desensitization) induced by the immunotherapy. The BDT can be used as an immunological parameter for IgG-monitoring of the course of venom immunotherapy and--in addition to skin tests and IgE-RAST--as a further criterion for deciding to stop venom therapy if it turns negative with the washed cells.

Adolescent↗
PubMed1987

[The basophil degranulation test in the diagnosis of Hymenoptera sting allergy].

In 98 patients with a suspected immediate type allergy to hymenoptera stings, skin tests (ST) with venom extracts, RAST determinations of venom specific serum IgE antibodies and basophil degranulation tests (BDT) with unwashed leukocytes were performed. BDT is a technically simple in-vitro method for detecting cell fixed IgE without risk for the patient. The specificity of BDT was investigated in a group of 10 controls not allergic to insect stings, of whom 7 were atopics. A good correlation could be demonstrated between BDT and the histamine release test (HRT), which was performed in the Institute for Allergy and Clinical Immunology, Inselspital, Bern. For the detection of a sensitization to insect venoms, ST with venom extracts were the most sensitive, followed by RAST and BDT; in patients whose allergic sting reactions dated back some time BDT was more frequently positive than RAST, which measures excessive serum IgE and not cell bound IgE responsible for the clinical symptomatology. As BDT--in the presence of patient serum--reflects the influences of different humoral (specific IgE, specific IgG) and cellular factors ("releasability"), it seems more appropriate to the actual clinical allergy situation than ST or RAST. Some case reports are presented to illustrate this. However, BDT does not represent an alternative to ST or RAST but a significant complement in diagnostically unclear cases and an aid in deciding to start specific venom immunotherapy, if history, ST and RAST are not sufficient. An appropriate score system ist proposed.

Adult↗
PubMed1988

[The human basophil degranulation test. Attempt at determining its reliability].

A single-blind assay was made between a laboratory in Paris and our own, in order to assess objectively the Human Basophil Degranulation test (TDBH). The study was made on 28 subjects. They presented with anaphylactic or systemic reactions to hymenoptera venom. The samples from these patients were sent to Paris without any other diagnostic information. Agreement of results between ANGERS and PARIS was very poor, at around 38.5%. Agreement between the TDBH at ANGERS and PARIS with other biological parameters and skin tests was also weak, at 41.7 to 55% in ANGERS and 45.8 to 58.3% in PARIS. The techniques used at ANGERS and PARIS were the same. Overall, TDBH does not seem to be either reliable or reproducible between two laboratories, in the special case of hypersensitivity to hymenoptera venoms.

Anaphylaxis↗
PubMedDate not supplied

[Clinical performance of the basophil degranulation test. Correlation with other diagnostic technics in allergy (II)].

Although the clinical history is the fundamental method for the diagnosis of reaginic diseases, the use of complementary techniques that gears on the detection of the humoral and cellular effector cells intervening in such affectation is necessary. Thus, we can detect the existence of specific IgE. It can be joined to the basophil membrane by means of techniques "in vivo" as in cutaneous tests, or by means of techniques "in vitro" through cellular techniques in which we apply a biochemical pattern (release of histamine) or a morphological one (HBDT). It can also be free in serum by means of the P-K test or "in vitro" by means of techniques of double antibody. We can also determine, by this last technique, the quantity of the total IgE in serum. In the present work, we review the human basophil degranulation test (HBDT) and its correlation with the prick test, the peripheral blood eosinophilia, the total serum IgE quantification by enzymatic technique and the specific IgE detection by isotope method. In our technique the test was considered positive when the degranulation index with known allergen concentration was greater than 30% for pollens and greater than 25% for D. pteronyssinus with a p less than 0.001. As reported in a previous work, we explain that the normal limits are slightly inferior to those used by some other authors, because in our series, the degranulation average of healthy controls is inferior to that reported by those authors which makes us consider the convenience that the normal limits of degranulation index should be established in a particular way by each technique and allergen.(ABSTRACT TRUNCATED AT 250 WORDS)

Basophils↗
PubMed1987

Human basophil degranulation test in liver hydatidosis.

Upon exposure to a specific antigen, granulation of basophils bearing IgE disappears. The aim of this study was to assess the human basophil degranulation (HBD) test with hydatic antigen in 100 patients. Seventy patients were clinically suspected of having liver hydatidosis (LH) (group I). Eight patients had asymptomatic hepatic calcifications. Twenty-two patients had previous surgery for LH (group III). In group I the percentage of HBD was significantly higher in patients with LH (23 of 70) than in patients with other hepatobiliary diseases (P less than 0.001). In group II all patients had negative HBD tests. In group III the test became negative within 8-12 months after radical surgery in four patients, and remained positive in the other 18. These results suggest that the HBD test is useful for the diagnosis of LH. In asymptomatic patients with hepatic calcified cyst, a negative HBD test suggests a dead parasite. In previously operated patients, a positive test suggests persistence of antigen.

Animals↗