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A review of helisoma duryi in biological control.

Biological control of schistosomiasis by means of introduction of the north American planorbid snail, Helisoma duryi, as a competitor of the intermediate host snails has been proposed. The systematics of the genus Helisoma and the geographic distribution of the different species is described. Papers dealing with laboratory experiments or field observations on the competition between H. duryi and different intermediate host snails have been reviewed. The status of H. duryi as intermediate host of trematodes has been evaluated by searching the literature for all the trematode species that are recorded from the genus Helisoma. The list does not include trematodes of medical or veterinary importance and despite many attempts it has not been possible to infect H. duryi with Schistosoma mansoni and S. haematobium. Finally this paper makes a few comments on the experiments that should be performed in the laboratory, under semifield conditions and and field conditions before H. duryi should be actively dispersed in Africa. The aspects to be considered include the nature of the competitive interactions, the relation between H. duryi and different medical and veterinary important trematodes and the effect of H. duryi on the biotope.

Animals

The role of helminths in the biological control of mammals.

Biological control of invertebrates has been successful while that of vertebrates has been, with the exception of myxomatosis in rabbits, unsuccessful; reasons for this are discussed. Demographic studies of small mammals suggest that population regulation occurs by several different mechanisms, more than one of which may be acting at the same time. Coevolution is an important phenomenon in host-parasite associations, nevertheless parasites may limit host population abundance. The basis of the regulatory effect on the host population is that parasite-induced host mortality or reduction in fecundity is density-dependent. Increasing evidence of the density-dependent effects of helminths on host survival and reproduction is forthcoming from laboratory studies but has not been confirmed in the field. The theory that a helminth parasite may regulate mammal population abundance has been verified recently in the laboratory. A multidisciplinary research programme aimed at understanding the mechanisms responsible for formation of house mouse (Mus domesticus) plagues and seeking strategies to reduce mouse numbers is discussed. One aspect of the work involves investigation of the potential of the nematode, Capillaria hepatica, as a biological agent in the control of wild mice in the cereal-growing regions of Australia. Biological control of mammals is viewed within the context of integrated pest management. A helminth species which reduces host survival or fecundity at an increasing rate as host abundance increases has a role in host population regulation. There is potential to capitalize on that role and apply the helminth as a biological agent in the control of mammals which have attained pest status.

Animals

Public health advantages of biological insect controls.

Biological control is not new, it is simply newly appreciated. This renewed appreciation stems from the widespread insecticide treadmill which is largely a product of insecticide disruption of the balance of insect communities. Biological control is a natural phenomenon; the regulation of plant and animal numbers by natural enemies. In this broad sense, biological control is vital to public health because it keeps the myriad insect species from out-competing us. It also has direct public health advantages as where natural enemies are manipulated to control disease vectoring insects. Insecticide distruption of biological control by insecticides and the resulting pesticide treadmill have serious public health implications. One is the increased pesticide load in the environment. The other is the acceleration of pesticide resistance in disease vectoring insects. The treadmill and its associated hazards will not abate so long as chemical control dominates our pest management strategy.

Environmental Health

Population biology and biological control of nematodes.

We studied the population biology of the nematophagous fungus Hirsutella rhossiliensis to understand its potential as a biological control agent. Because the fungus is an infectious and transmissible parasite, we framed our study within an epidemiological context. Field observations, theory, and experiments demonstrated that (i) parasitism of nematodes by H. rhossiliensis is dependent on nematode density, (ii) local populations of the fungus will go extinct unless supplied with some minimum number of nematodes (the host threshold density), and (iii) natural epidemics of this fungus in populations of nematodes develop slowly and only after long periods of high host density. Additional in-depth research on population biology is needed to explain other biological control systems and to guide future research. The most effective research will combine field observation, theory, and experimentation.

Animals

Biological control of insect pests affecting man and animals in the tropics.

Biological control of pests affecting the health of man and animals is practiced in various forms throughout the tropics. In this paper, the use of parasitic viruses, bacteria, protozoa, predatory arthropods, and fish against pests such as various mosquitoes, tse tse flies, and screwworm flies as published in the literature are reviewed. Mention is also made of the usefulness and applicability of the sterile insect technique, genetic control by chromosomal aberrations, and the exploitation of various incompatabilities. These are reviewed against the background of the present state of technology and limited resources that exist in many tropical countries. Most authors maintain that due to the relative length of time required to get a biological control system working efficiently, and the perennial nature of most tropical pest species, there is often the need to initially reduce the pest population by conventional means. There will thus be a balance between biological and chemical control in most systems. Emphasis is placed on meeting the urgent need for the exchange of research and development information on biological control of pests affecting man and his animals in the tropics.

Animals

Monoclonal antibodies to Gliocladium roseum, a potential biological control fungus of sap-staining fungi in wood.

Immunological probes were developed to discriminate between a potential biological control fungus and sap-staining fungi present in wood. This paper describes the production of monoclonal antibodies to isolated cell wall fragments of the biological control fungus Gliocladium roseum. Two monoclonals, designated 6A5 and 3F12, were characterized. Their specificity was assessed by ELISA, by immunogold silver staining light microscopy, by immunogold electron microscopy, and by immunoblotting. Monoclonal 6A5 specifically recognized G. roseum and closely related species and did not react with any of 21 sap-staining fungi tested. Monoclonal 3F12 recognized most of the biological control fungi tested and also showed reactivity with two of the 21 sap-staining fungi. Both monoclonals appeared to recognize carbohydrate epitopes of the cell wall in G. roseum. Although the antibodies were produced against the cell wall of fungus grown in liquid culture, they also detected specific fungi in wood and, therefore, can be used for studies of wood colonization by fungi and for investigations of the interactions between different fungi growing on wood.

Antibodies, Fungal

Biological control of the snail hosts of Schistosoma mansoni in the Caribbean area using Thiara spp.

Field observations and experiments using thiarid snails as competitors of Biomphalaria spp., potential intermediate hosts of Schistosoma mansoni in the Caribbean area, are reviewed. The parthenogenetic snails, Thiara granifera and T. (= Melanoides) tuberculata, were introduced to the Neotropical area in recent decades. In numerous islands and countries, these oriental species have demonstrated their capacity to colonize rapidly and densely many types of habitats while at the same time reducing and even eliminating populations of Biomphalaria spp. The results of field experiments, carried out in several Caribbean islands, have shown the efficiency as well as the limitations of T. tuberculata as a competitor of B. glabrata and B. straminea. In St. Lucia, B. glabrata was apparently eliminated from marshes and streams, 6 to 22 months after the introduction of the competitor. In Martinique, T. tuberculata was introduced into two groups of water-cress beds which constituted the last transmission sites of schistosomiasis on the island. In just less than three years after the introduction of the competitor, both B. glabrata and B. straminea have been eliminated from the transmission sites. In Guadeloupe, several introductions have been carried out in different types of habitat such as permanent ponds, canals, streams and temporary marshes. The findings of all field experiments have indicated that thiarid snails as competitors of pulmonates are favoured by the presence of permanent and stable habitats, preferably shallow, with emergent plants and well oxygenated. On the other hand, the competitor snails are at a disadvantage in waterbodies which are temporary, extremely deep, poorly oxygenated or with a dense mat of floating aquatic vegetation.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals

Biological control of Penicillium digitatum by Trichoderma viride on postharvest citrus fruits.

In previous studies it was shown that Trichoderma viride, isolated from Spanish citrus packing houses, showed antagonistic activity against Penicillium digitatum in in vitro laboratory tests. In the present in vivo studies Navelina oranges, protected with aqueous suspension of T. viride (2.5 x 10(6) to 2.5 x 10(9) spores per ml), showed an increase in resistance toward P. digitatum. Oranges, inoculated with P. digitatum, did not produce lesions after 5 days when T. viride was applied 48 h or 72 h before inoculation.

Citrus

Commercial and naturally occurring fly parasitoids (Hymenoptera: Pteromalidae) as biological control agents of stable flies and house flies (Diptera: Muscidae) on California dairies.

Filth fly parasites reared by commercial insectaries were released on two dairies (MO, DG) in southern California to determine their effect on populations of house flies, Musca domestica L., and stable flies, Stomoxys calcitrans (L.). Spalangia endius Walker, Muscidifurax raptorellus Kogan and Legner, and Muscidifurax zaraptor Kogan and Legner were released on the MO dairy from 1985 to 1987 in varying quantities. Parasitism by Muscidifurax zaraptor on the MO dairy was significantly higher (P less than 0.05) from the field-collected stable fly (4.4%) and house fly (12.5%) pupae, compared with a control dairy (0.1%, stable fly; 1.3%, house fly). Muscidifurax zaraptor, released from April through October during 1987 on the DG dairy (350,000 per month), was not recovered in a significantly higher proportion from either fly species relative to the corresponding control dairy. No specimens of Muscidifurax raptorellus were recovered from the MO dairy. Parasite treatments had no apparent effect on adult populations of either fly species or on overall parasitism rate of field-collected stable fly (16.8%, MO; 17.2%, DG) and house fly (23.3%, MO; 20.9%, DG) pupae. Spalangia spp. were the predominant parasites recovered from field-collected stable fly and house fly pupae on all four dairies. Sentinel house fly pupae placed in fly-breeding sites on both release dairies were parasitized at a significantly higher rate, as compared with sentinel pupae on control dairies. The generic composition of parasites emerging from sentinel house fly pupae was 20.6% Spalangia spp. and 73.2% Muscidifurax spp., whereas in field-collected house fly pupae, Spalangia spp. and Muscidifurax spp. constituted 74.3 and 19.6% of the parasites, respectively.

Animals

Biological control of Culex mosquitoes (Diptera: Culicidae) by the tadpole shrimp, Triops longicaudatus (Notostraca: Triopsidae).

Laboratory oviposition choice tests and behavioral observations indicated that the activity of tadpole shrimp, Triops longicaudatus (LeConte), near the water surface deterred gravid Culex quinquefasciatus Say from ovipositing. In the cities of Oasis and Riverside, Calif., tadpole shrimp significantly reduced the abundance of immature mosquitoes (Cx. tarsalis Coquillett and Cx. quinquefasciatus) probably due to lowered oviposition rates, as well as tadpole shrimp predation. Generally, mosquito oviposition rates in field ponds with tadpole shrimp were lower than that of controls, except when tadpole shrimp were very young (4 d after flooding) or when their abundance had declined late in the flooding period. When analyzed by pond, tadpole shrimp size was correlated inversely with abundance; however, differences in size or abundance did not affect their capacity to reduce mosquito populations.

Animals

Biological control of container-breeding mosquitoes, Aedes albopictus and Culex quinquefasciatus, in a Japanese island by release of Toxorhynchites splendens adults.

To control container-breeding mosquitoes in the small island of Minnajima (0.56 km2), northern Okinawa, Japan, laboratory-reared adults (aged 7-10 days) of Toxorhynchites splendens (Palawan strain), a mosquito with predatory larvae, were released repeatedly during 1984, 1986 and 1987. Thirteen species of mosquitoes (Diptera: Culicidae) occurred in artificial containers, ground pools or crab-holes on the island, the predominant species being Aedes (Stegomyia) albopictus and Culex (Culex) quinquefasciatus. Predatory mosquito larvae of Culex (Lutzia) fuscanus and Cx (Lt.) halifaxii were found commonly in wet containers. In the first year of study, during a period of 54 days from 13 May to 5 July 1984, totals of 879 female and 806 male adults of Tx.splendens were released on six occasions. Similarly, between 29 April and 30 August 1986, totals of 2920 female and 2878 male adult Tx.splendens were released. In the third study year, totals of 2041 female and 1783 male Tx.splendens were released on eight occasions during 199 days from 23 April to 7 November 1987. After adult releases at two sites, the immature stages of Tx.splendens were found in 164 out of 502 traps in 1984, 421 out of 933 traps in 1986, and 151 out of 502 traps in 1987. The number of immatures of Tx.splendens present in each trap varied from 1 to 40 in 1984, 1 to 29 in 1986 and 1 to 9 in 1987. Numbers of immatures of the target species found in the traps during August-September averaged 71.9/trap/month in 1984, 114.7/trap/month in 1986 and 36.0/trap/month in 1987, significantly less in the traps with Tx.splendens than in those without them. The present field studies indicated that, in this small island, approximately 250 adult female and 200 male Tx.splendens per month should be released from April to November, and the releases should be carried out every year, in order to control effectively the target mosquitoes Ae.albopictus and Cx quinquefasciatus breeding in artificial containers in Minnajima.

Aedes

Expression of the mosquitocidal toxins of Bacillus sphaericus and Bacillus thuringiensis subsp. israelensis by recombinant Caulobacter crescentus, a vehicle for biological control of aquatic insect larvae.

In the quest for effective control of mosquitoes, attention has turned increasingly to strains of the bacteria Bacillus sphaericus and Bacillus thuringiensis subsp. israelensis, which produce potent toxins with specific mosquitocidal activities. However, sedimentation of the bacterial spores limits the duration of effective control after field application of these bacilli. We describe here the cloning of genes encoding the 51.4- and 41.9-kDa toxins from B. sphaericus 2297, the 100-kDa toxin from B. sphaericus SSII-1, and the 130-kDa toxin from B. thuringiensis subsp. israelensis into the broad-host-range plasmid pRK248 and the transfer of these genes for expression in Caulobacter crescentus CB15. The recombinant C. crescentus cells were shown to be toxic to mosquito larvae. Caulobacter species are ubiquitous microorganisms residing in the upper regions of aquatic environments and therefore provide the potential for prolonged control by maintaining mosquitocidal toxins in larval feeding zones.

Animals