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At least 19 recordsLinked to original sources
PubMed1979

Pitfalls in collecting blood specimens for antibiotic assay.

Obtaining blood samples for antibiotic analysis from intravenous lines through which the antibiotic had been administered was critically evaluated. Flushing volumes required to remove contaminating antibiotic varied with the type of antibiotic and with different plastics. Obtaining blood specimens from the intravenous lines is not practical in infants and not advised in adults.

Adult↗
PubMed1978

[The technic of arterial blood-specimen collection in cattle].

A simple method of collecting arterial blood in cattle by puncture of the arteria iliaca externa per rectum is described. Individual collections do not induce any local reaction nor do they impair the overall condition and milk yield of the animals.

Animals↗
PubMed1979

Manual fluorometry of phenylalanine from blood specimens collected on filter paper: a modified procedure.

In this manual fluorometric method, blood samples are used that have been impregnated on the filter paper, a convenient collection technique that is widely used to screen newborns for phenylketonuria. The modified procedure, based on the method of McCaman and Robins [J. Lab Clin. Med. 59, 885 (1962)], includes elution of phenylalanine from specimens on filter paper, removal of proteins by precipitation with trichloroacetic acid at 0 degrees C, and then reaction with ninhydrin-peptide reagent for color development. The standard curve is linear to at least 200 mg/L and the CV is 6.3% for a phenylalanine concentration of 27 mg/L. The modified procedure is suitable both for early screening for phenylketonuria and for monitoring blood phenylalanine of phenylketonurics during dietary therapy.

Blood Specimen Collection↗
PubMed1978

[Contribution to the morphology and biochemistry of mustelide blood. 1. Blood specimen collection in various mustelides with special reference to the use of the tissue adhesive "Fimomed"].

An account is given of various techniques of blood sampling in the context of certain mustelids, including cardiopuncture, amputation of tail tip, talon cutting, incision of the ear vein, puncture of the jugular or femoral vein, and catheterisation of the abdominal aorta. Reference is made to details of use of all techniques, characteristics, advantages and potential setbacks, and preferable use of some of the tested methods to collect blood from mustela and martes species. Blood collection from the abdominal aorta may be helpful in obtaining no-haemolysis and no-additive plasma for biochemical multi-screening. Biochemical, pharmacological, and toxicological follow-up checks may be feasible under certain conditions following surgical exposure of the external jugular vein. The use of "Fimomed" (n-butylcyano-acrylate), a tissue adhesive, may help to reduce effort in terms of time and material and consequently, rationalise veterinary hygiene action as a whole, provided that the conditions for its application are observed. The skin adhesive is properly applicable to skin lesions of mustelids. A combination of suturing with adhesive should be used to close laparotomy wounds for better mechanical strength of the abdominal wall. Possible applications of "Fimomed" should be tested with other species as well.

Animals↗
PubMed1977

Free erythrocyte porphyrins in the detection of undue absorption of Pb and of Fe deficiency.

A fluorometric assay for free erythrocyte porphyrins (FEP) is described in detail, the direct test being used for the analysis of blood specimens collected with anticoagulants and the spot test for analysis of blood specimens collected on filter paper. A method for determining the amount of blood absorbed by different lots of filter paper is described.

Anemia, Hypochromic↗
PubMed1978

Evaluation of a finger prick blood collection method for the seroepidemiology of hepatitis B.

A finger prick-swab method of blood specimen collection was qualitatively and quantitatively compared with the conventional venipuncture method for HBsAg and anti-HBs determinations by radioimmunoassay (RIA). The new method consisted of pricking the finger, collecting 0.1-0.2 ml of blood with a cotton-wool swab, and eluting the swab in 1 ml of 1% bovine albumin in saline containing 0.1% sodium azide. Using chimpanzees seropositive for HBsAg or anti-HBs, comparisons were made of RIA results of: (a) whole blood, haemolysed blood, serum, and plasma; (b) paired finger prick samples and serum; (c) dilutions of finger prick samples and serum; and (d) different volumes of blood on swabs. Field studies were carried out at two institutions where hepatitis B was hyperendemic to compare results from paired finger prick and serum specimens assayed by the RIA and haemagglutination techniques. The laboratory studies showed that swab RIA values for anti-HBs were significantly lower than serum values and that for HBsAg, swab values were significantly higher than serum values. In HBsAg tests, the field studies showed 100% agreement between the two methods; in anti-HBs tests, the finger prick method showed 85% agreement with positive sera. Because of the logistics of collecting and processing blood serum, the finger prick-swab technique may be a valuable aid in large-scale seroepidemiological surveys for hepatitis B.

Antibodies, Viral↗
PubMed1977

The blood groups and other heriditary blood factors of Yemenite and Kurdish Jews.

Blood specimens collected fro Yemenite and Kurdish Jews living in Israel were tested for 11 blood group systems 5 plasma protein systems and 9 systems of red-cell enzymes. The results of these tests were combined with those of tests on other Yemenite and Kurdish Jews, reported by Godber et al. (1973), the total data sorted according to the place of origin of the subjects or their parents in the Yemen Arab Republic and Kurdistan respectively. Gene frequencies were calculated for each of the local populations so defined. It is confirmed that the Yemenite Jews show a close relationship to the Yemenite Arabs, but those from the southern part of the Yemen Arab Republic have a higher frequency of African marker genes than those in the north. The Habbanite Jews have a similar rather high frequency of African genes (Bonné et al., 1970). The Kurdish Jews from Iran and northern-western Iraq show a moderate genetic resemblance to the indigenous Kurds of Iran, while those from south-eastern Iraq differ considerably, especially in their low frequency of A1, high B, high CDe (R1) and low cde (r).

Blood Group Antigens↗