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At least 19 recordsLinked to original sources
PubMed1983

Presence of alkaline phosphatase and gamma-glutamyl transpeptidase on the parietal layer of Bowman's capsule.

Generally, the parietal layer of Bowman's capsule in the mammal kidney consists of a squamous epithelium resting upon a basement lamina. However, tall cylindrical cells resembling those of the proximal tubules were observed on the outer wall of Bowman's capsule in the mouse and rat kidneys. These cells were provided with an apical brush border and were positive for alkaline phosphatase and gamma-glutamyl transpeptidase suggesting phosphate and amino acid transport at the capsular level.

Alkaline Phosphatase↗
PubMed1979

[Sex difference and fine structure on epithelium cells of Bowman's capsule in mice (author's transl)].

Bowman's capsules with cuboidal epithelium were found in 87.2%, 95.5%, 75.4%, 96.7%, 98.8% and 91.2% in the kidney of adult male C57BL/6J-ob, JCL-ICR, dd, E1, ASK and KK mice, respectively, while in 57.9%, 6.7%, 7.0%, 2.8% and 9.1% in adult female C57BL/6J-ob, JCL-ICR, dd, E1 and ASK mice, respectively. The renal corpuscles with cuboidal epithelium in Bowman's capsules of male JCL-ICR mice increased gradually in number from 5 to 10 weeks age. The surface of cuboidal epithelium cells was shown to have long cilia and dense microvilli, which were similar to those seen in proximal uriniferous tubules.

Animals↗
PubMed2000

Protein gene product 9.5 is selectively localized in parietal epithelial cells of Bowman's capsule in the rat kidney.

Parietal epithelial cells (PEC) of Bowman's capsules cover the inner aspect of Bowman's capsules and are believed to contribute to extracapillary lesions of glomerulonephritis such as crescent formation. In glomerular research including cell culture experiments and pathology, differentiation between PEC and podocytes has frequently been a major problem. Immunohistochemistry of the adult rat kidney for protein gene product 9.5 (PGP 9.5), a neuron-specific ubiquitin C-terminal hydrolase, demonstrated selective localization of the immunoreactivity in PEC. At the urinary pole of the glomerulus, immunoreactive PEC were clearly differentiated from proximal tubular cells that were negative for PGP 9.5. In the subcapsular nephrogenic zone of newborn rat kidney, immunoreactivity was observed in almost all cells in the commashaped body and early S-shaped body and selectively in PEC in the late S-shaped body and capillary-stage glomerulus. In rat glomerular disease models (Masugi-nephritis and puromycin aminonucleoside nephrosis), cells that consisted of cellular crescents or adhered to glomerular tufts were positive for PGP 9.5. The selective localization of PGP 9.5 in PEC in rat kidney provides a new cytochemical marker for identifying the cells. Development expression of the protein suggests that PGP 9. 5 is involved in the processes of nephrogenesis of rat kidney.

Aging↗
PubMed1994

Immune complex deposition in Bowman's capsule is associated with parietal podocytes.

We have recently documented the presence of podocytes lining part of Bowman's capsule at the vascular pole, in adult human kidney. In this study, we describe the deposition of immune complexes in Bowman's capsule in association with these parietal podocytes. We examined 1 year's consecutive human renal biopsies (n = 170). Transmission electron microscopy (TEM) revealed 18 cases in which parietal podocytes were present. Of these 18, there were 11 cases of glomerulonephritis, in which immune complexes were demonstrated in the capillary tuft by both TEM and direct immunofluorescence microscopy. In seven of these 11 cases, TEM showed immune complex-type deposits in Bowman's capsule, always associated with parietal podocytes. These deposits were similar in size, appearance, and distribution to the deposits in the capillary tuft. By contrast, non-specific electron densities within Bowman's capsule were found beneath both squamous parietal cells and parietal podocytes. In four cases, Bowman's capsule also showed focal positive immunostaining for complement components and/or fibrinogen. Both parietal and visceral podocytes showed similar fusion of pedicles. We suggest that filtration through parietal podocytes may be responsible for immune complex deposition and subsequent damage to the vascular pole of the glomerulus in human renal disease.

Antigen-Antibody Complex↗
PubMed1976

Cell junctions in the epithelium of Bowman's capsule.

The intercellular connections between the epithelial cells of Bowman's capsule were investigated. It could be demonstrated that typical zonulae occludentes (tight junctions) are present in the species (rat, hamster, and Tupaia) studied. Freeze-fracturing shows a network of anastomizing strands; some species variations are described. In the rat two strands are common. In the golden hamster mostly two to four and occasionally five strands occur. In Tupaia regularly three tight junction strands are found and also gap junctions associated with the zonulae occludentes. In thin sections the goniometric analysis confirms the freeze-fracturing results and reveals attachment zones of macular shape, which are classified as intermediate junctions and desmosomes. The functional role of these cell junctions observed in the epithelium of Bowman's capsule is discussed.

Animals↗
PubMed1986

Metaplasia of the parietal layer of Bowman's capsule: a histopathological survey of the human kidney.

Human kidney sections taken at autopsy were examined to determine the incidence of metaplasia of the Bowman's parietal epithelium. Autopsy records were consulted to determine if there was any correlation between clinical disease, histopathological changes in organ systems and metaplasia of Bowman's capsule. The sections represented both sexes in 9 age groups from 2 to 87 years. The sections were fixed in neutral formalin, embedded in paraffin, sectioned at 6 microns, and stained with hematoxylin and eosin. A total of 129 kidney sections, representing 129 individuals, were evaluated. One hundred renal corpuscles were counted per section and the parietal layer of Bowman's capsule was classified as normal (squamous) or metaplastic (cuboidal). Of the 129 kidneys examined, 69 (53%) had metaplasia of Bowman's capsule. Of the 87 male kidneys, 51 (59%) exhibited metaplasia of Bowman's capsule. Of the 42 female kidneys examined, 18 (43%) of the kidneys had metaplasia of Bowman's capsule. On average, in kidneys with metaplasia, 4% of the renal corpuscles had metaplasia of Bowman's parietal layer. The lesion was present in both sexes in all age groups. The autopsy records revealed that there was no common clinical condition associated with the metaplastic lesion, but metaplasia of Bowman's parietal epithelium was consistently present with hepatic congestion and/or fatty changes.

Adolescent↗
PubMed1984

Sex and strain differences in mouse kidney: Bowman's capsule morphology and susceptibility to chloroform.

Chloroform (CHCl3) produces liver damage in male and female mice but renal injury only in male mice. Previous reports demonstrated that the parietal epithelium of Bowman's capsule in female mice is composed of squamous cells, whereas the parietal cells of Bowman's capsule from male mice consist of a mixture of squamous and cuboidal cells or purely cuboidal cells. These data suggested that the factor(s) associated with these histological differences may similarly be associated with factors that determine susceptibility to tubular damage from chloroform. The susceptibility of male mice to chloroform nephrotoxicity varies with animal strain. In the present study, administration of CHCl3 to male C57BL/6J (C57) and male and female DBA/2J (DBA) mice produced dose-dependent kidney tubular damage in male C57 and male DBA mice. However, CHCl3 nephrotoxicity was considerably greater in male DBA mice than in male C57 mice. Female DBA mice failed to develop renal damage following administration of CHCl3. Male DBA mice had a higher percentage of cuboidal parietal epithelial cells in Bowman's capsule whereas male C57 mice had a higher percentage of squamous cells in the parietal epithelium. The parietal epithelium in Bowman's capsule of female DBA mice was predominantly squamous. Since morphological differences between sex and strain of mice occurred in the capsular epithelium, and the differences in susceptibility to CHCl3 appear to be a tubular phenomenon, it is unlikely that there is a causal relationship between the structure of Bowman's capsule and chloroform-induced renal tubular necrosis; rather, these two independent phenomena are regulated by some common factor(s), possibly testosterone.

Alanine Transaminase↗
PubMed1999

Distribution of actin bundles in Bowman's capsule of rat kidney.

In this study we define the distribution of actin bundle arrangement in Bowman's capsule of rat renal corpuscles. Parietal cells of Bowman's capsule were examined by conventional light microscopy, electron microscopy and confocal microscopy. Within each parietal cell individual actin bundles are arranged in a parallel fashion running the length of the cell. Computer reconstructions obtained using confocal microscopy clearly show the lengths of actin bundles to be arranged, on a capsule level, end-to-end, at angles and perpendicular to bundles in adjacent cells. The bundles stain positively for non-muscle myosin and vinculin. The presence and arrangement of actin bundles in parietal cells is consistent with a role in reinforcing capsule structure.

Actins↗
PubMed1988

Metaplasia of the parietal layer of Bowman's capsule in the human kidney: incidence in alcoholic liver disease and hypertension.

This report is the second of two surveys to determine the incidence of metaplasia of Bowman's parietal epithelium in the human kidney. Human kidney sections obtained at autopsy at the Department of Pathology, University of Texas Medical Branch, Galveston, Texas, were examined with the light microscope. The kidneys were fixed in neutral formalin, sectioned at 6 microns and stained with hematoxylin and eosin. Autopsy records were consulted after kidney section examination to determine if there was any correlation between clinical disease, histopathological changes in organ systems and metaplasia of Bowman's capsule. The kidney sections represented both sexes in 8 age groups, from less than one year to 80 years. A total of 174 kidneys, representing 174 individuals, were evaluated. One hundred renal corpuscles were counted per section and the parietal layer of Bowman's capsule was classified as normal (squamous) or metaplastic (cuboidal). Of the 174 kidneys examined, 137 (79%)--79 male and 58 female--had metaplasia of Bowman's capsule. On the average, in the kidneys with the lesion, 6% of the renal corpuscles had metaplasia of Bowman's parietal layer. The lesion was present in both sexes in all age groups. The autopsy records revealed that metaplasia of Bowman's parietal epithelium was usually present with hepatic fatty changes and/or congestion. Alcoholic liver disease and hypertension represented the most frequent clinical diseases in the sample; these conditions had the highest incidence of metaplasia. Twenty-six of the 174 kidney samples were from individuals with alcoholic liver disease, all of whom had metaplasia of Bowman's capsule.(ABSTRACT TRUNCATED AT 250 WORDS)

Adolescent↗
PubMed1970

Tubular metaplasia in Bowman's capsule.

Proximal tubular type metaplasia of the epithelium of Bowman's capsule is described in a fit young adult male. This form of metaplasia is unlike those previously described and could cause initial confusion if encountered in a percutaneous renal biopsy.

Adult↗
PubMed1988

The ultrastructural organization of the basement membrane of Bowman's capsule in the rat renal corpuscle.

The basement membrane of Bowman's capsule (BCBM) of the rat was studied by means of a modified tissue-preservation technique for transmission electron microscopy, which avoids the usual thorough fixation in OsO4 and applies tannic acid and uranyl acetate for staining (Sakai et al. 1986). At most sites the BCBM is multilayered, consisting of one to seven dense layers separated by electron-lucent layers. The latter, which can be termed laminae rarae, contain fine filaments which connect the dense layers to each other and the innermost dense layer to the basal cell membrane of the parietal epithelium. The laminae densae are basically composed of fine filaments arranged in an anastomosing pattern. Individual filaments ranging from 5 to 15 nm in diameter, combine to form filament bundles up to 100 nm in thickness and 1 to 2 micron in length. Within a dense layer, filaments and filamentous bundles are oriented mainly in the same direction. Often the inner dense layers do not form a continuous sheet, and the filamentous bundles are arranged in anastomosing or spiral patterns to form a ribbon-like structure that we call a "microligament". These microligaments are often embedded in basal furrows of the parietal epithelium and are best developed around the vascular pole. Intracellular actin bundles of the parietal cells are regularly associated with these extracellular ribbon-like structures of the basement membrane. In conclusion, the BCBM has an unusual structure: the laminae densae are characterized by their filamentous nature and are arranged in different patterns, i.e. as a multilayered mat and as microligaments.

Animals↗
PubMed1981

The origin of proliferating cells in the glomerulus and Bowman's capsule in nephrotoxic serum nephritis: effects of unilateral renal irradiation.

The origin of proliferating cells in glomeruli and Bowman's capsules during nephrotoxic nephritis (NTN) in rabbits was studied, using unilateral renal irradiation to suppress glomerular cell division. Left (LI) kidneys received 2400-12,000 rad on Days 1-5 after i.v. nephrotoxic serum (NTS) 1 or 1.5 ml/kg body wt. Renal histology, cell labelling 1 h after a pulse of tritiated thymidine (H3T) (0.5 mCi/kg), and mitotic rate were compared in right non-irradiated (RO) kidneys and LI kidneys. Differences in H3T uptake were not significantly suppressed (P less than 0.05) by 4800 rad whereas mitoses in glomeruli and Bowman's capsule were not. Glomerular hypercellularity and crescents were equivalent in LI given 4800 rad and in RO kidneys, but fibrin deposition was increased 2-fold in LI kidneys. In control rabbits (no NTS) LI kidneys showed no histological changes. The results suggest that most dividing cells detected in glomeruli and Bowman's space in NTN are monocytes, and that these cells form a large component of crescents.

Animals↗
PubMed1988

Peri-hilar rupture of Bowman's capsule in crescentic glomerulonephritis.

Crescents are an important structure in many forms of glomerulonephritis and a major cause of destruction of the renal corpuscle. There has been considerable discussion of the cellular content of crescents and of the role of breaks in the glomerular capillary basement membranes. Less attention has been given to abnormalities of Bowman's capsule. We present 2 cases of anti-gbm nephritis showing unusual breaks in Bowman's capsule with a peri-hilar distribution. The mechanisms leading to the rupture of Bowman's capsule are reviewed, as are the possible effect of capsular ruptures on the crescentic cell content.

Adult↗
PubMed1982

Proximal-tubule-like epithelium in Bowman's capsule in spontaneously hypertensive rats. Changes with age.

Kidneys were samples from male spontaneously hypertensive rats (SHR) and normotensive rats (WKY) in four groups. Renal tissues were examined in 64 rats: 6 SHR and 6 WKY rats 8 and 16 weeks of age and 10 SHR and 10 WKY rats 32 and 64 weeks of age. Tissue samples were fixed, processed, and stained by routine histologic procedures. The parietal layer of Bowman's capsule in 100-115 renal corpuscles from right to left kidney sections was classified as squamous or cuboidal epithelium. The cuboidal epithelium was similar in structure to that of the proximal tubule. Quantitative information from right and left kidneys was pooled, because the data did not differ significantly. The percentages of renal corpuscles with proximal tubule-like epithelium present at the parietal layer of Bowman's capsule in the SHR was 13%, 35%, 44%, and 81% at 8, 16, 32, and 64 weeks, respectively. In WKY rats the values were 4%, 0.5%, 5%, and 13% at 8, 16, 32, and 64 weeks, respectively. The increase in the percentage of renal corpuscles with proximal tubule-like epithelium in SHR Bowman's capsules suggest an association between this tissue and hypertension. The modified layer of Bowman's capsule may be a response to an increase in blood pressure, may have some role in the etiology of hypertension, or may be irrelevant to hypertension.

Aging↗
PubMed1979

Autoantibody specific for the glomerular mesangium and Bowman's capsule in man.

Investigation of serum from a nominally healthy subject revealed an unusual autoantibody with specificity for the glomerular mesangium and Bowman's capsule. Immunofluorescence studies on rodent kidney sections revealed typical mesangial fluorescence and intense linear staining of Bowman's capsule. The antibody was organ, but not species, specific and did not correspond to any previously described antibody with anti-mesangial activity (e.g. anti-actomyosin or anti-fibronectin). Intravenous injection in mice resulted in heavy mesangial deposition of antibody within one hour, the antibody disappeared within 2--4 weeks, and no urinary abnormalities were seen. Retrospective analysis of clinical data revealed a history of chronic hepatitis and possible drug abuse, but no evidence for impaired renal function at any timepoint. To our knowledge this is the first report of specific anti-mesangial antibody in man, the pathogenetic relevance remains unclear.

Animals↗