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At least 19 recordsLinked to original sources
PubMed1988

Assessment of Brucellosis Card test in screening patients for brucellosis.

The Brucellosis Card test (Brewers' Diagnostic Kits, Hynson, Westcott and Dunning, Inc., Baltimore, Md.) was evaluated in relation to the Brucelloslide test (bioMérieux, France), the microagglutination test (MAT) and the demonstration of brucella-specific IgG, IgM and IgA in an enzyme-linked immunosorbent assay (ELISA). A total of 573 serum specimens was tested. These included sera from patients with acute brucellosis (159), chronic brucellosis (23) and patients who had been diagnosed previously as having had brucella infection (155). Control groups consisted of patients with diseases other than brucellosis (52), others with non-infectious diseases (20), and healthy individuals (164). The Card test detected 100% of the patients with acute and 61% of the patients with chronic brucellosis. The sera from the control groups were all negative. Similar results were obtained with the Brucelloslide test and the MAT. The ELISA test detected brucella-specific Ig of all classes in the serum of patients with acute brucellosis, and IgG and IgA in the serum of patients with chronic brucellosis. In the latter group, IgM was also detected in 32% of the sera. Twenty-three per cent of sera with titres of 20 by the MAT were positive on the Card test and had ELISA titres for IgM, IgG and IgA of 400. Characterization of the antibodies involved in the Card test showed that sera with IgM ELISA titres of 1600, or an IgM titres of 800 together with IgG and IgA titres greater than or equal to 200 were Card test positive. Higher IgG (greater than or equal to 1600] plus IgA (greater than or equal to 400) titres were required to produce a positive Card test in the absence of IgM or when the IgM titre was less than or equal to 200. The Card test has a potential value as a rapid screening test for humans with acute brucellosis and shows similar results to Brucelloslide and MAT tests. ELISA, however, remains the most reliable test for diagnosis of brucellosis especially in patients with chronic and complicated stages of the disease.

Acute Disease↗
PubMed1991

[Activity of adenosine deaminase in acute brucellosis and complicated brucellosis].

BACKGROUND: Adenosine deaminase (ADA) is an essential enzyme for the differentiation and proliferation of T lymphocytes and the monocyte-macrophage system. The basic immunitary response of brucellosis is cellular. To this end, ADA activity was evaluated in brucellosis. METHODS: Serum ADA activity was assessed by a colorimetric method in 67 patients with brucellosis, before therapy and at 1, 3 and 6 months of follow up. RESULTS: Serum ADA activity in brucellosis was higher than that in 52 healthy controls, both in those with the acute febrile noncomplicated form (48 patients) and in those with focal symptoms from one organ (19 patients) (p less than 0.0001 and p less than 0.001). There were no differences between both groups of brucellosis. There was a negative correlation between the duration of the disease and ADA activity. After therapy there was a rapid decrease of ADA values, more marked in patients with noncomplicated brucellosis. During the follow up, only one patient had a new increase in ADA activity, coincident with a clinical and bacteriological relapse, and previous to the increase of IgG anti-Brucella titers. CONCLUSIONS: The results indicate that adenosine deaminase activity is increased during the active stage of brucellosis. It can be considered as a biochemical follow up marker of the disease and, probably, as a marker of relapses.

Acute Disease↗
PubMedDate not supplied

[Professional brucellosis and endemic brucellosis in a given area].

An epidemiological survey was carried out in an area where there is an abattoir/canning factory in which a recrudescence of cases of professional brucellosis was observed: whilst there had been 29 cases of brucellosis in 8 years, 10 cases were confirmed in 1970. Our survey had underlined a brucellosis endemy in the population of that area: 32.50% of the patients had agglutinating or complement-fixing antibodies (698 people were examined). Results obtained have brought us to discuss the professional origin of brucellosis.

Abattoirs↗
PubMed1975

[Diagnosis of human active or latent human brucellosis by lymphocyte transformation. Inhibition of leukocyte migration and passive hemagglutination. Possible interference between brucellosis and some mental disorders].

Passive hemagglutination tests (H.T.), involving the coating of a soluble B. abortus antigen onto sheep red blood cells through chromium chloride, were always negative in non-brucellic subjects. Positive H.T., even at low titers (1:50), were associated with positive specific lymphoblastic (T.T.L.) and inhibition of leucocyte migration (I.M.L.) tests in 13 patients. These 3 tests were negative in 17 control brucellosis-free individuals. Positive H.T. confirmed a clinical diagnosis of brucellosis in 27 patients with dubious or negative responses to classical tests. Low positive (1:50, 1:100) H.T. may correspond to Brucella primary-infection or to a quiescent chronic infection, and higher titers to clinically active brucellosis. There is a correlation between cellular immunity tests (T.T.L., I.M.L.) and passive hemagglutination test. Furthermore, 39/88 women hospitalized in psychiatric wards were positive to H.T., and 6 of them were also highly positive to complement fixation and tube agglutination tests. Three T.T.L. and I.M.L., performed on samples from 3 H.T. (1:50) positive patients, were positive. These 30 patients were classified as depression or severe anorexia.

Adult↗
PubMed1986

Evaluation of ELISA in the diagnosis of acute and chronic brucellosis in human beings.

An enzyme-linked immunosorbent assay (ELISA) was used to determine the levels of brucella-specific IgG, IgM and IgA in 173 patients with acute brucellosis, 22 patients with chronic brucellosis and in 281 controls consisting of 98 patients with other infectious etiologies, 20 patients with non-infectious diseases and 163 normal healthy adults. The ELISA results were compared with culture findings, the results of slide agglutination tests with Brucella melitensis (M), B. abortus (A) and Ross Bengal (RB) antigens, and of tube and microagglutination tests. Brucella cultures were positive in 53 and 5% of patients with acute and chronic brucellosis respectively. The slide agglutination tests with A, M, A plus M and RB antigens were positive in 42, 44, 51 and 98% of patients with acute brucellosis and in 23, 27, 27 and 64% of patients with chronic brucellosis. There was no significant difference in the results between the tube and microagglutination tests regardless of the type of antigen used. At a titre of greater than or equal to 80 or greater than or equal to 160 these tests were positive in 98% and 92% of patients with acute brucellosis and 60 and 40% of patients with chronic brucellosis. The brucella culture and agglutination tests were negative for all the controls. Brucella ELISA immunoglobulins (Ig) were detected in some individuals in the control groups but the majority of these had titres of less than or equal to 100 for IgG, IgM, and IgA. However, patients with brucellosis had significantly higher ELISA titres in all classes of Ig than controls but the sensitivity and specificity within each Ig class varied with the titre considered. At a titre of greater than or equal to 1600 the brucella IgG had a sensitivity and specificity of 98% for patients with acute or chronic brucellosis; this decreased with lower reciprocal titres. The brucella IgM titre of greater than or equal to 400 had a sensitivity of 98% and a specificity of 98% for patients with acute brucellosis. However, in patients with chronic brucellosis the brucella IgM was very low. The brucella IgA titre of greater than or equal to 200 showed a sensitivity of 98% and a specificity of 99% for patients with either acute or chronic brucellosis. This study indicates that brucella ELISA is a rapid, sensitive and specific assay, provides a profile of Ig classes in the diagnosis of acute and chronic brucellosis, is useful for mass screening and could be considered the method of choice for the serological diagnosis of brucellosis.

Acute Disease↗
PubMed1976

[Clinical and epizootiological aspects of bovine, caprine, and ovine brucellosis in Greece].

The author reports on an investigation, in Greece, of the clinical and epizootiological aspects of bovine, caprine and ovine brucellosis. These three types of brucellosis are found in the abortive and latent forms. The abortive form is revealed by contagious abortions whose frequency depends principally on the composition of the animal population of the farm; during its evolution, numerous very high positive serological reactions are observed. It involves many complications, causes heavy economic losses and is passed on to a great number of breeders. The latent form is accompanied by chronic manifestations, but passes unnoticed for the most part and is discovered only by serological tests during a period of suspect abortions of human cases of brucellosis. There are fewer of these cases then when the disease evolves in the region in the abortive form. The latent form contributes to the multiplication of centres of infection (1) when apparently uninfected female animals coming from farms contaminated with the latent form are introduced into healthy herds, (2) when the latent form changes into the abortive form after the introduction of healthy female animals into farms contaminated with this form. Furthermore, because of its inapparent evolution, it is a constant danger to rural populations. Bovine brucellosis has been observed in 16 of the 52 departments in the country, and caprine and ovine brucellosis in 21. The proportion of infection throughout the bovine, caprine and ovine herds of the 38 departments in which the investigation was made is approximately 4.70, 1.29, and 0.33 per thousand, respectively. The usual pathogenic agent of bovine brucellosis is Br. abortus; Br. abortus resistant to thionin is found less frequently and Br. melitensis only occasionally. The usual pathogenic agent of caprine and ovine brucellosis is Br. melitensis; Br. intermedia is less frequent and Br. abortus is found only rarely. Four Tables show the clinical investigation of 150 bovine abortions of brucellic origin and observations on the sanitary conditions of 22 cattle-sheds and herds infected with abortive or latent brucellosis. The author concludes in favour of the necessity of medical prophylactic measures and the strict application of sanitary prophylactic measures. In general, new animals should not be introduced into farms but when this is necessary the animal should not be considered free from brucellosis simply because of negative serological reactions. The best policy, in this case, is to repopulate entire farms negative to serological tests by seronegative animals coming also from entirely negative farms.

Abortion, Veterinary↗
PubMed1990

Specific IgE response in patients with brucellosis.

In the search to find discriminative serological markers to differentiate between patients with acute brucellosis and those with chronic brucellosis, an enzyme-linked immunosorbent assay (ELISA) was used to determine and compare the brucella-specific IgE response in 80 sera from patients with acute brucellosis, 37 sera from patients with chronic brucellosis, 26 sera from patients with positive blood cultures for bacteria other than brucella and 51 sera from healthy controls. The IgE findings were compared to brucella-specific IgG, IgM, IgA and IgG1-4 demonstrated by ELISA, and to microagglutination test (MAT) results. Elevated (positive) antibrucella IgE titres were detected in 89 and 81% of sera from patients with acute and chronic brucellosis respectively. The predominant antibodies found in patients with acute brucellosis were of the IgG, IgM, IgA, IgE, IgG1 and IgG3 types while in chronic brucellosis IgG, IgA, IgE and IgG4 were found. Although IgE can be detected in patients with brucellosis, it does not discriminate between the acute and chronic stages of the disease.

Acute Disease↗