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Glucuronidase activation: enzyme action at an interface.

The potentiating action of chloroform on bacterial beta-glucuronidase has been shown to increase as the interface area between the two liquid phases increases. Prior extraction of the enzyme with chloroform causes a loss rather than an increase in activity. It is tentatively suggested that the correlation between activity and interface area may reflect a phenomenon of enzyme action at a liquid/liquid interface.

Chloroform↗