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At least 19 recordsLinked to original sources
PubMed1962

Spectrophotometry of the periodic acid-Schiff reaction with pituitary hormones in vitro and in histological sections.

The spectral light absorption of the in vitro periodic acid-Schiff reactions of 4 purified pituitary hormones is described. The absorption spectra present a maximum between 560 and 565 mmicro. The color developed conforms with Beer's law for the ranges of concentration examined. The different hormones exhibit different chromogenicity per unit of biological activity: the color produced by 1 unit of FSH is equivalent to approximately 2 of TSH, 4 of LH, and 30 of ACTH. Microspectrophotometric measurements of the PAS-positive structures in histological sections of the human pituitary give absorption curves with shapes similar to those obtained in vitro, although quantitative differences exist. It is concluded that under the proper experimental conditions microspectra of the pituitary structures might, in the future, prove to give a quantitative measure of aldehyde groups generated from glycoprotein tropins by periodate oxidation.

Adrenocorticotropic Hormone↗
PubMed1960

The quantitative assay of corticotrophin using rats treated with hydrocortisone acetate.

An attempt has been made to modify the Sayers method for the assay of corticotrophin by substituting for hypophysectomy an injection of sufficient hydrocortisone acetate to suppress the release of endogenous pituitary corticotrophin in intact rats over the period of the assay. The results of forty such assays of subcutaneous corticotrophin seem to indicate that this procedure is about 1(1/2) to 2 times as efficient as the original method using hypophysectomized rats. For a 2 + 2 point assay it was necessary to use a minimum of twenty-four to thirty-two rats.

Adrenocorticotropic Hormone↗