[In vivo and in vitro studies on penetration behavior of human spermatozoa thorough cervix mucus].
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An overlook is given about the theory of penetrating tests. The assay of tests depends from some biological factors concerning sperms, spermplasma and the cervix mucus itself. The penetrating tests are important in functional disorders in the area of cervixsperm. In negative results hormonal defects and immunological factors have to be considered.
The bacteriologic study of the cervical flora in 50 healthy women revealed that 70% harbored mixed aerobic and anaerobic organisms. Aerobic organisms alone were recovered in 15 women (30%). Polymicrobial organisms were found in all but five women (in these only one aerobe was isolated per patient). The majority of patients had 1-2 anaerobes, with more than two aerobes. The common aerobes were alpha-hemolytic streptococci, Staphylococcus epidermidis and lactobacilli. The most common anaerobes were Peptococcus asaccharolyticus isolated in 21 women (42%), P prevotii in 13 (26%) and Bacteroides in 10 (20%). These organisms are frequently found in pelvic infection, suggesting the pathogenic potential of the normal flora of the cervix.
A simple method is described to collect cervical smears and to have a clear view of cervix for small breed of monkeys. This method was found useful to collect adequate cytology smears with good preservation of cellular morphology.
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A method for scanning electron microscopic examination of exfoliated ectocervical cells smeared with a cotton swab is described. It is concluded that this technique has advantages over techniques in which cell samples are collected from the portio by washing, scraping, or adherence to membranes.
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In 40 couples with negative penetration test in vivo and in vitro we have looked for abSA, abSI and abIF. We discuss the usefulness of the condom protected intercourse and the artificial insemination with husband's sperm as therapeutic approaches.
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