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At least 19 recordsLinked to original sources
PubMed1976

Demonstration of mast cell granules by the cetylpyridinium chloride-acid dye (CPC-AD) and cetylpyridinium chloride-phosphotungstic acid (CPC-PTA) methods.

Cetylpyridinium chloride (CPC) and cetyltrimethylammonium bromide (CETAB) are bound to polyanionic substances by ionic bonds between the positively charged nitrogen of the quaternary salts and the negative groups of polyanions. The mast cell granules and some other structures treated with CPC or CETAB react selectively with acid dyes and fluorochromes. In ultrathin sections treated with CPC, phosphotungstic acid (PTA) greatly enhances the electron density of the granules of mast cells. The possible mechanism of acid dye and PTA binding by CPC or CETAB treated tissues is discussed.

Animals↗
PubMed1979

Influence of cetylpyridinium chloride on corneal permeability to penicillin.

The epithelial surface or the deepithelialized anterior stromal surface of isolated rabbit corneas was perfused for 3 hr with 14C-penicillin in 25 mM Ringer-bicarbonate solution with or without 1% albumin and with or without 0.02% cetylpyridinium chloride. The intact epithelium acted as a barrier to penicillin and impeded the flux rate by 66% when compared to the flux rate across the deepithelialized cornea. The presence of 0.02% cetylpyridinium chloride increased the penicillin flux rate across corneas with an intact epithelial layer to that of deepithelialized corneas. Cetylpyridinium chloride, 0.02%, had no effect on penicillin flux across deepithelialized corneas. The penicillin flux rate across corneas, with or without epithelium, was increased slightly following the inclusion of 1.0% albumin in the bathing solution. The flux rates across deepithelialized corneas in the presence of albumin, with or without cetylpyridinium chloride, were similar to fluxes found in the absence of albumin. Albumin-penicillin "binding" was not a significant factor in impeding penicillin flux, and this binding apparently was not altered by cetylpyridinium chloride. The surfactant appeared to alter epithelial permeability physiologically.

Animals↗
PubMed1976

Effects of two cetylpyridinium chloride-containing mouthwashes on bacterial plaque.

The purpose of this single blind clinical study was to determine the effects of two commerical mouthwashes (one containing cetylpyridinium chloride and domiphen bromide and the other containing only cetlypridinium chloride) on existing plaque accumulations. A second purpose was to determine if a residual effect could be shown 2 weeks after cessation of using these mouthwashes. A total of 120 adult subjects, who had been divided into three groups, were initially in the study. For a period of 31 days, the subjects in Group A rinsed with a commercial mouthwash containing cetylpyridinium chloride and domiphen bromide; Group B rinsed with a commercial mouthwash containing only ceytlpyridinium chloride; and Group C served as controls and rinsed with colored flavored water. All subjects continued their normal home oral hygiene practices, except that mothwashes other than as assigned were forbidden. The subjects received three identical examinations to determine their plaque scores. The examinations were conducted the day preceding initiation; the day following cessation; and 15 days after cessation of the rinsing procedures. A total of 105 subjects received the first and second examinations, and 93 subjects received all three examinations. Based upon the data obtained, the daily use of each of the cetylpyridinium chloride mouthwashes tested, appears to be partially effective in reducing existing bacterial plaque accumulations. The cetylpyridinium chloride-domophen bromide product was slightly, but not significantly, more effective than the other commercial mouthwash. Neither commerical product demonstrated a significant residual effect 2 weeks after cessation of use.

Adolescent↗
PubMed1976

Electrophysiological and anatomical effects of cetylpyridinium chloride on the rabbit cornea.

The effects of cetylpyridinium chloride on the trans-corneal potential difference and the surface anatomy of the cornea have been examined. Concentrations of cetylpyridinium chloride from 0.21 mM to 2 mM were used for either 1 or 2 minute exposure times on the in vitro and in vivo cornea for the electrophysiology studies. The potential difference of the in vitro cornea showed a concentration and exposure-time dependent decrease, the in vivo cornea shows a qualitatively similar behaviour although quantitatively less. The fall in potential difference is preceeded by a hyperpolarization. The scanning electromicroscopy reveals a loss of microvilli and microplicae as well as surface pitting, with some exposure of cells underlying the superficial epithelium. These changes occur in a dose-dependent manner. The effect of cetylpyridinium chloride on the cornea is to enhance the permeability of the superficial cells by destroying the cell membranes and causing lysis of the cells.

Action Potentials↗
PubMed1976

Radiosensitization of Serratia marcescens by cetylpyridinium chloride. Evidence for membrane-associated events.

Cetylpyridinium chloride has been shown to be an effective radiosensitizer of both oxic and anoxic suspensions of Serratia marcescens in buffer. The related compounds ethylpyridinium bromide and cetyltrimethylammonium chloride exhibited no such radiosensitizing properties at comparable concentrations. It is suggested that the efficiency of cetylpyridinium chloride is due to the combination of lipid-soluble (cetyl) and electron-affinic (pyridinium) moieties within the same molecule, and that these may provide for interaction with a membrane-associated target. Cetylpyridinium chloride did not radiosensitize bacteria suspended in nutrient broth.

Cell Membrane↗
PubMed1975

Use of cetylpyridinium chloride and sodium chloride for the decontamination of sputum specimens that are transported to the laboratory for the isolation of Mycobacterium tuberculosis.

A method is presented for the decontamination, liquefaction, and concentration of sputum specimens that are in transport more than 24 h. The method is inexpensive, and culture results compare well with those obtained with the accepted N-acetyl-L-cysteine and sodium hydroxide method for the isolation of tubercle bacilli. The working solution, 1% cetylpyridinium chloride and 2% sodium chloride, is mixed in equal volumes with sputum before the specimens are shipped. Tubercle bacilli remained viable after 8 days of exposure to this solution. Only Lowenstein-Jensen medium was used because the cetylpyridinium chloride in the inoculum remains active on 7H10 or other agar base media and partially inhibits the growth of tubercle bacilli.

Acetylcysteine↗
PubMed1977

Isolation and physical characterization of hyaluronic acid prepared from bovine nasal septum by cetylpyridinium chloride precipitation.

Raw extract in 2 m CaCl2 of bovine nasal septum cartilage was eluted from 4 per cent agarose gel to give a "void volume" Fraction v-4, which was indistinguishable in composition and behavior on viscometric and sedimentation analysis from the densest fraction obtained by associative centrifugation in a cesium chloride density gradient. The sulfated proteoglycan was precipitated (Fraction A) by cetylpyridinium chloride from acidic solutions of Fraction v-4 or of dialyzed raw ectract. Neutralization under conditions of low ionic strength precipitated a further small fraction (B), which contained from 0.5 to 1 per cent of the uronic acid in the original extract. Analysis by associative and dissociative density gradient centrifugation demonstrated that Fraction B resembled in effective density known samples of hyaluronic acid from other sources. Gel chromatography of proteolytic digests of Fractions A and B on 6 per cent agarose indicated that cetylpyridinium chloride precipitation essentially separated sulfated proteoglycan (A) from hyaluronic acid (B). A viscosity-average molecular weight of about 5 x 10(5) was estimated for a sample of Fraction B purified in a dissociative (4 M guanidine hydrochloride + CsCl) density gradient. Sedimentation velocity data were consistent with this result. Analysis of hexosamines showed that the sample contained 96 per cent glucosamine, confirming the identification of hyaluronic acid. The proteoglycan fraction (A) resembled "subunits" in its sedimentation behavior.

Amino Acids↗
PubMed1978

Binding of dyes to hydroxyapatite treated with cetylpyridinium chloride or cetrimonium bromide.

The effect of cetylpyridinium chloride (CPC) and cetrimonium bromide (CTAB) on the adsorption of some acidic food dyes to hydroxyapatite was studied. The dyes investigated were brilliant blue (FD&C Blue No. 1), tartrazine (FD&C Yellow No. 5), sunset yellow (FD&C Yellow No. 6) and amaranth (FD&C Red No. 2). The apatite had adsorbed 9.2 mumol CPC per g dry weight. The adsorbed CPC was in equilibrium with a free concentration of 20 microgram/ml (58 micrometer). The adsorption of CPC and CTAB to the apatite was followed by an increased ability of the crystals to bind the dyes. The dyes were very firmly adsorbed and were not released during a series of washings. Untreated apatite showed only a minor affinity for the dyes. The adsorbed dyes were easily washed out. CPC and CTAB showed the smae specific ability to increase the binding capacity of the apatite. The results are discussed and related to the formation of stains on the teeth in persons using quaternary ammonium compounds for mouthrinsing. A mechanism explaining the production of stains is proposed.

Adsorption↗
PubMed1978

In vitro studies on the use of cetylpyridinium chloride as a bacterial plaque control agent.

Studies have shown that cetylpyridinium chloride (CPC) containing mouthwash may in vitro inhibit artificial bacterial plaque accumulation under certain conditions. These studies, together with the findings of clinical trials, suggest that the clinical activity of CPC in partially limiting plaque accumulation depends on its adherence to cleaned enamel surfaces rather than its penetration in performed plaque.

Adsorption↗
PubMed1975

Rapid determination of the amount of cetylpyridinium chloride bound by bacteria.

A modification of the Colowick and Womack procedure for measuring ligand binding by macromolecules is described for drug binding by bacteria. This technique is based on the determination of drug concentration in the dialysate from a bacteria-drug mixture at equilibrium. The dialysis cell of the original procedure was replaced by a Minibeaker (Bio-Rad), which has a larger membrane surface area, and the dialysate was continuously monitored with a spectrophotometer equipped with a flow cell. With this system, only 3 min was required to determine the amount of cetylpyridinium chloride bound by Escherichia coli K-12 strain P678. Possible applications of the technique are discussed.

Absorption↗
PubMed1976

Effect of cetylpyridinium chloride on pathogenic fungi and Nocardia asteroides in sputum.

The effect of cetylpyridinium chloride (CPC) on pathogenic fungi and Nocardia asteroides was studied. Sputa seeded with each of 11 organisms (Aspergillus flavus; Aspergillus fumigatus, Blastomyces dermatitidis, Candida albicans, Coccidioides immitis, Cryptococcus neoformans, Geotrichum candidum, Histoplasma capsulatum; Nocardia asteroides, Paracoccidioides brasiliensis, and Sporothrix schenckii) were treated with CPC and kept for 2, 5 and 9 days. The CPC reagent used (0.5% CPC and 0.5% sodium chloride) is one the Mycobacteriolgoy Branch at the Center for Disease Control added to sputa before shipping them to laboratories for recovery of mycobacteria. None of the organisms tested survived this treatment, and none was recovered on mycological or mycobacteriological media. Seeded sputa containing these organisms were also tested with a second CPC reagent (0.02% CPC and 0.5% sodium chloride) and held for 2, 5 and 9 days. A few colonies of A. flavus, A. fumigatus, and N. asteroides were recovered from these samples. Neither the morphology of the fungi nor their stainability by the fluorescent antibody method was affected by treatment with the reagent containing 0.5% CPC. However, the background material in smears from the 0.5% CPC-treated samples retained the conjugate, and this made weakly fluorescing organisms more difficult to detect. The 0.5% CPC treatment did not alter the morphology of N. asteroides or its ability to be stained with Kinyoun acid-fast stain.

Cetylpyridinium↗
PubMed1977

[Inhibition by "essential" phospholipids of the bactericidal effect of cetylpyridinium chloride on common bacteria. Short communication].

The authors examined the conditions under which the bactericidal action of the disinfectant cetylpyridinium chloride (CPC) is neutralized by a dispersion of "essential" phospholipids (EPL). Both gram-positive and gram-negative strains of bacteria were exposed to various concentrations of the quarternary ammonium over different periods of time. Three cultures were prepared for each contact model to check on the effect of either pre-treatment and after-treatment with phospholipids or of CPC-exposure without the addition of phospholipids. The results obtained demonstrate that EPL are able to "receive" gram-negative bacteria like E. coli, P. aerug. and Morax. glucidol. after exposure to lethal concentrations of CPC for 30 min. While the "phenomenon of restoration" depended on the dose of phospholipids applied, the time of expsure to phospholipids proved irrelevant. The stabilisation of bacterial membranes due to EPL is discussed as a possible explanation of the phenomenon of restoration.

Bacteria↗