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At least 19 recordsLinked to original sources
PubMed1979

On the chromaffin system of the African lungfish, Protopterus aethiopicus.

The distribution of chromaffin tissue was studied in the African lungfish using Falck-Hillarp fluorescent histochemistry together with quantitative analysis of catecholamines in plasma and tissue extracts. Intensely fluorescent cells form chromaffin tissue in the wall of the atrium, the wall of the most anterior part of the left cardinal vein and the walls of the segmentally arranged intercostal arteries. The arrangement thus appears to be a combination of the situations in cyclostomes, elasmobranchs and teleosts. Adrenaline is present in larger quantities than noradrenaline in the intercostal arteries and the cardinal vein, while noradrenaline dominates in the atrium. During "stress" induced by physical disturbance of the animals, a strong increase in especially the noradrenaline concentration of the plasma was detected.

Animals↗
PubMed1977

Monoamine-storing cells in the avian aortic wall.

Using the Falck-Hillarp method, monoamine-storing cells were demonstrated in the avian thoracic aorta just above the openings of the ductus arteriosus. By electron microscopy, numerous dense-cored vesicles, 90-220 nm in diameter, were seen in the cytoplasm. The electron opacity of these dense-cored vesicles was reduced when reserpine (5 mg/kg) was administered. These monoamine-storing cells formed typical synapses with the Schwann cell-enclosed nerve terminals. The monoamine-storing cells first appeared in the aortic wall at 9 days in ovo but it remained obscure whether they originated from the neuroectoderm.

Animals↗
PubMed1975

A glutaraldehyde/potassium dichromate tracing method for the localization and preservation of abdominal extra-adrenal chromaffin tissues.

The present work introduces a method for the localization in situ of the abdominal paraganglia. After treating retroperitoneal tissue blocks with a near-neutral glutaraldehyde/potassium dichromate solution following routine glutaraldehyde perfusion, intra- and extraadrenal chromaffin tissues develop a pronounced brown color from the interaction of glutaraldehyde/potassium dichromate with amines. In this manner, visualization of the abdominal extra-adrenal chromaffin organs is enhanced at the same time that cellular ultrastructure is preserved. Subsequent examination of the dichromate-reacted tissues with the electron microscope confirms that they represent the amine-rich paraganglia. This method offers an effective alternative to extensive sampling of plastic-embedded blocks for localizing peripheral chromaffin tissue and has been used to define the exact distribution of abdominal paraganglia in the rabbit.

Abdomen↗
PubMed1979

Light scattering turbidity changes as a measure of the kinetics of Ca2+ -promoted aggregation of chromaffin granule membrane ghosts.

Changes in turbidity seen when chromaffin granule membrane ghosts are aggregated by Ca2+ can be modelled as dimerization of hollow spheres using Rayleigh-Gans-Debye light-scattering theory. The experimental changes agree well with the calculations. Thus, if shape or refractive index changes produced by osmotic perturbation, ion uptake, etc. can be excluded, turbidity readings can be used to follow the progress of the aggregation reaction of storage vesicles and other small particles or macromolecules.

Animals↗
PubMed1978

The endocrine nature of the paranganglia of man.

Brightly fluorescent paraganglia were found in the retroperitoneal tissue of adult man. The histofluorescence properties of the paraganglia indicate the presence of tryptophyl peptides, which might be of endocrine importance.

Animals↗
PubMed1979

[Histological study of the paracervical ganglion (Frankenhäuser) in mice (author's transl)].

The paracervical ganglion in dd-mice ranging in age from birth to 90 days was studied qualitatively and quantitatively by light microscopy. The paracervical ganglion is located just lateral to the fornix vaginae, and is shaped like a flattened cone. Out of the parenchymal cells, nerve cells are 97% and chromaffin cells are 3%, respectively. The chromaffin cells are arranged in small clusters or individually, and are scattered throughout the ganglion. Their chromaffin reaction varies in density from cell to cell. In neonatal mice, the ganglion consists of nerve cells and small spherical cells. With advancing age, as the small cells decrease in number, chromaffin cells appear. At 20 days of age, the ganglion is almost similar in structure to that of the adult. The nuclear and cellular size distribution curves of nerve cells are symmetrical in pattern after the age of 20 days. The nuclear size distribution curves of the chromaffin cells are symmetrical and are almost the same in pattern in newborns as in adults. The cellular size distribution curve of chromaffin cells are symmetrical before 20 days of age, but, with advancing age, the curve shifts to the larger size and becomes asymmetrical in pattern due to an increase of larger cells.

Age Factors↗
PubMed1975

A study of the distribution of chromaffin-positive (CH+) and small intensely fluorescent (SIF) cells in sympathetic ganglia of the rat at various ages.

Small intensely fluorescent (SIF) and chromaffin-positive (CH+) cells were independently investigated by formol-induced fluorescence and by chromaffin techniques in the superior cervical, thoracic and coeliac-mesenteric ganglia of neonatal (2--10 days), adolescent (2--4 months) and adult (6--15 months) rats. Identification of CH+ cells was facilitated by glutaraldehyde fixation prior to chromatin. Intraganglionic blood vessels were displaced by antemortem injection of either India ink or the fluorescent dye Thioflavine-S. SIF and CH+ cells were randomly distributed through the ganglia, either singly or in pairs related to principal neurons, or in variably-sized, highly vascularized groups. In chromaffin preparations these groups either consisted entirely of CH+ cells or else they contained a mixture of CH+ and CH- cells. CH+ cells were present in some adolescent and adult ganglia of all types, and in the neonatal coeliac-mesenteric ganglion at 10 days. In neonatal material generally, SIF cells were mostly green fluorescent, occurring separately or in homogeneous or mixed groups, but both yellow and green cells occurred in coeliac-mesenteric ganglia at 10 days. All ganglia in adolescent and older animals contained both yellow and green cells. There were more green than yellow cells, and more SIF than CH+ cells in all ganglia studied.

Age Factors↗
PubMed1979

Ultrastructure and possible function of an atrial paraganglion not directly related to atrial ganglion in the albino mouse.

The ultrastructure of a paraganglion found in right atrium of albino mouse close to the superior cava vein is described. A capillary, which occupied the central part of the paraganglion, was surrounded by three chromaffin cells. Two other chromaffin cells were also present, but neither nerve fibers nor nerve endings were seen. The possible physiological role of paraganglia is discussed.

Albinism↗
PubMed1978

The distribution and endocrine nature of the abdominal paraganglia of adult man.

The paraganglia of adult man were studied using the formaldehyde-induced fluorescence (FIF) method for histochemical characterization of biogenic monoamines. Microspectrofluorimetry was used to record the emission spectra and fluorescence intensities of the paraganglionic cells. The study of samples from six patients showed that well vascularized paraganglia were widely distributed throughout the retroperitoneal spaces. The paraganglia exhibited strong FIF with the spectral characteristics of monamines. Treatment with HC1 caused an increase in the fluorescence intensity of the paraganglia and a simultaneous shift of the emission maximum from 480--495 nm. This change suggests the presence of high concentrations of tryptophyl-containing peptides and is not due to monoamines. The possibility of a dual endocrine function for the paraganglia is discussed.

Abdomen↗
PubMed1978

Paraganglia of the bladder.

Normal paraganglia containing high amounts of catecholamines are a constant feature of the wall of the bladder in elderly men. The paraganglia in this location might give rise to pheochromocytomas. The importance of the local endocrine effects in the regulation of the muscular tone in the bladder is discussed.

Chromaffin System↗