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At least 19 recordsLinked to original sources
PubMed1979

[Studies of bovine Mycoplasma mastitis. 2. Testing of various culture media and culture methods for the isolation of Mycoplasma from milk samples].

The recipes Medium-I broth, Medium-B broth, and Weissenseemycoplasma broth as well as Medium-I agar, Medium-B agar, and MRL agar were tested for their applicability to culturing mycoplasma from milk samples, using the direct and indirect techniques. No dependable information on the occurrence of mycoplasma was obtainable from tested material unless several nutritive media and techniques were combined. Medium I, for which almost no imported substances were needed, was in no way inferior to common international nutritive substrates. Its use in conjunction with the indirect culturing technique is recommended for routine diagnosis.

Animals↗
PubMed1979

Evaluation of ten anaerobic blood culture media.

Selection of an anaerobic blood culture based upon clinical findings that have compared the isolation rates of bacteremic agents from different blood culture media. No agreement has been reached as to which of the commercially available blood culture media is optimal for detection of bacteremia. The purpose of this study was to determine the rates of recovery of anaerobic microorganisms from various anaerobic blood culture media. The blood culture media were inoculated with a small inoculum of microorganisms in the presence or absence of an erythrocyte-serum mixture. The results demonstrated that the type of medium and the erythrocyte-serum mixture influenced the ability of blood culture media to support the growth of microorganisms. The majority of the media failed to support the growth of 87% or more of the microorganisms within four days after inoculation. Pre-reduced brain-heart infusion broth supported the growth of a larger proportion of microorganisms than the other types of blood culture media.

Anaerobiosis↗
PubMed1978

Evaluation of twenty-three blood culture media.

Several investigators have evaluated clinically a variety of commercially available blood culture media. No agreement has been reached as to which of these media is optimal for detection of bacteremia. The purpose of this study was to determine the rate of recovery of microorganisms from various blood culture media. A total of 23 blood culture media were inoculated with 7 to 15 microorganisms per bottle in the presence or absence of an erythrocyte-serum mixture. The results demonstrated that blood culture media differed in their ability to support the growth of microorganisms. At 4 days after inoculation, only 10 of the 23 blood culture media supported the growth of 91% (10 of the 11) or more of the test microorganisms. The recovery rate of microorganisms depended not only upon the type of medium but also upon the manufacturer of the type of blood culture medium. The addition of an erythrocyte-serum mixture to the blood culture media did not influence the difference in the recovery rate of microorganisms among media and the same type of medium prepared by different manufacturers. The majority (15 of the 23) of the blood culture media supplemented with the erythrocyte-serum mixture failed to support the growth of 91% or more of the test microorganisms at 4 days after inoculation. These results have demonstrated that blood culture media need to be improved. Better quality control measures should also be implemented to evaluate commercial blood culture media.

Bacteria↗
PubMed1975

Influence of oxygen and culture media on maintenance of whole hamster trachea organ cultures and replication of Sendai virus.

Effects of various oxygen concentrations and culture media on the maintenance of 4-day-old hamster trachea organ cultures and the yield of Sendai virus were studied. The basic media used were Eagle minimal essential medium, medium 199, and CMRL 1066 supplemented with glutamine and antibiotics and buffered with NaHCO3 or N-2-hydroxyethyl-piperazine-N'-2'-ethanesulfonic acid (HEPES). In addition, each medium was evaluated under a gas phase of 5% CO2 and 95% O2, 5% CO2 and 45% O2 and 50% N2, or 5% CO2 and 95% air. Culturing of explants with CMRL 1066 and medium 199 buffered with HEPES in the presence of 5% CO2 and air proved most efficient; ciliary movement and ciliated surface epithelium were maintained for periods up to 27 days. No significant difference in the rate of replication of Sendai virus was seen in the three different media with the two buffer systems in the three different gaseous phases. The addition of 0.2% bovine serum albumin to the media yielded greater quantities of virus, up to 200-fold increase in titer without producing changes in ciliary function. A distinctive pattern of morphological changes was observed in explants of trachea epithelia inoculated with Sendai virus. These results suggest the practical application in the use of whole hamster trachea explants as a diagnostic aid in the isolation of Sendai virus from laboratory rodents.

Animals↗
PubMed1977

Microcalorimetry as a tool for evaluation of blood culture media.

Evaluation of optimal compositions of blood culture media has called for extensive and laborious work in comparative studies of large series of clincal specimens. Bacterial growth is accompanied by heat production, and calorimetry provides an analytical tool for its detection and quantification. A twin microcalorimeter of the heat conduction type was used to register heat effects in experimentally infected blood cultures. When studying Escherichia coli and Staphylococcus aureus, larger heat effects were produced with 0.05% sodium polyanetholsulfonate than with 600 IU of heparin per ml, which was also the case when using 10% sucrose. The addition of IsoVitaleX (BBL) increased the heat effects produced by the two species mentioned, whereas it had the opposite effect in cultures of Neisseria meningitidis. The present study indicates that microcalorimetry is a valuable and time-saving tool for the evaluation of optimal compositions of bacterial culture media.

Blood↗
PubMed1975

[Obtaining additional agglutinins by mixing culture media of lymphoid tissues taken from animals immunized with Salmonella paratyphi B].

Culture media of splenic explants from chicken immunized with Salmonella paratyphi B have antiflagellar agglutinin titers which vary with time. When the culture media coming from distinct cultures in the same series are mixed on the same day of the culture, the agglutinin titer sometimes rises and becomes greater than the highest individual titer.

Agglutinins↗
PubMed1979

The primary isolation of Leishmania donovani from Iraq on different culture media.

Leishmania donovani was isolated by culturing bone marrow aspirates from kala-azar patients, on three types of culture media. The isolation was successful in 88% of the cases. The effects of pH, D-glucose, L-proline and antibiotic concentration in the isolation were investigated. The semi-solid medium used (pH 7.4) with L-proline, was better than the modified NNN media for the primary isolation.

Bone Marrow↗
PubMed1977

Erythropoietin, thrombopoietin and colony stimulating factor in fetal mouse liver culture media.

Fetal mouse livers, days 13 to 19 of gestation, were cultured for 21 days and the culture media tested for erythropoietin (Ep), thrombopoietin (TSF) and colony stimulating factor (CSF). High levels of both Ep and CSF were released into the culture media. However, no detectable TSF was found. Maximum Ep culture activity was detected in day 14 and day 15 fetal liver cultures while maximum CSF was found in the day 16 fetal liver culture. These studies indicate that fetal liver cells in culture are capable of producing and/or releasing both Ep and CSF but not TSF.

Animals↗
PubMed1978

Isolated mouse pancreatic islets in culture: effects of serum and different culture media on the insulin production of the islets.

Various conditions for tissue culture of collangenase-isolated mouse pancreatic islets were studied in an attempt to optimize the maintenance of glucose stimulated insulin biosynthesis and release in the cultured specimens. Islets which had been cultured at a physiological glucose concentration (5.5 mmol/1) in the absence of serum had an impaired glucose-stimulated insulin biosynthesis and release as well as a reduced insulin content. Thus, insulin biosynthesis was three times higher after culture in a serum supplemented medium. Further, the insulin secretion of islets cultured in the presence of serum was markedly enhanced in acute incubations with high concentrations of glucose. This response was most pronounced in islets which had been cultured free-floating. A comparison between different culture media showed that islets cultured in RPMI 1640 had the highest insulin production. The present data suggest that the most favourable conditions for long-term storage of isolated islets in culture may be obtained when the islets are maintained as free-floating explants in a culture medium consisting of RPMI 1640 supplemented with serum.

Animals↗
PubMed1979

Recovery of Haemophilus influenzae from twenty-three blood culture media.

Haemophilus influenzae is an important agent of bacteremia and has fastidious growth requirements. The purpose of this investigation was to determine the ability of commercial blood culture media to support the growth of this fastidious microorganism. Twenty-three types of blood culture media were inoculated with individual suspensions of eight strains of H. influenzae in the presence or absence of an erythrocyte-serum mixture. The rates of recovery of the H. influenzae strains from the various types of blood culture media were compared. The results demonstrated that the type of medium, the manufacturer, the erythrocyte-serum mixture, and the strain of H. influenzae influenced the recovery rates of H. influenzae. Optimal recovery of the strains of H. influenzae was obtained from brain heart infustion blood culture medium (GIBCO). Trypic soy broth (GIBCO) and supplemental peptone of Becton, Dickinson and Co. also were found to be superior to the remaining types of media tested for the recovery of H. influenzae.

Blood↗
PubMed1975

Comparison of hemoglobin-free culture media and thayer-martin medium for the primary isolation of Neisseria gonorrhoeae.

Translucent culture media are essential for the determination of gonococcal colony types. In this study two hemoglobin-free, translucent culture media were compared with Thayer-Martin (TM) medium for the primary isolation of Neisseria gonorrhoeae from male urethral specimens. The antibiotic-free translucent medium (GCB) and the translucent medium containing vancomycin, colistin, and nystatin (VCN) were as efficient as TM medium in detecting positive specimens. In fact, VCN medium gave positive results significantly more often than TM medium(P less than 0.05). The amount of growth on positive plates was comparable for all three media. Contaminants were noted most often on GCB medium (P less than 0.0001), and the frequency of contaminants with TM plates exceeded that of VCN plates (P less than 0.0001). The high isolation rate of N. gonorrhoeae with VCN hemoglobin-free culture medium and its lower cost and greater ease of preparation make it a suitable alternative to TM medium for use with male urethral specimens.

Colistin↗
PubMed1978

Determination of thymidine in serum used for cell culture media.

Thymidine concentrations in serum used for cell culture media were determined with an assay based on isotope dilution. In this assay, incorporation of (3H)-thymidine into DNA of cultured cells was measured in the presence of 5 and 20% serum as a function of the concentration of unlabeled thymidine added to the medium. In three batches of horse serum, thymidine concentrations were 0--0.17 micron, while in fetal calf serum values of 0.75--2.1 micron were obtained. Dialysis of serum resulted in a reduction of thymidine levels by factors of at least 10.

Cells, Cultured↗
PubMed1977

Detection of bacterial metabolites in spent culture media and body fluids by electron capture gas-liquid chromatography.

Electron capture gas-liquid chromatography, when used to analyze derivatized extracts of spent culture media and body fluids under specified conditions, holds promise as a tool for use by physicians, hospitals, and clinical laboratories in identifying certain diseases and disease-producing organisms. The detection of certain disease processes and the identification of disease-producing organisms are based on qualitative or large quantitative differences in EC-GLC profiles or a combination of both. Various practical procedures are given for extracting and derivatizing compounds, such as carboxylic acids, hydroxy acids, alcohols, amines, and nitrosamines. The characteristics of the parameters essential for successful analysis are discussed. Species and, in some cases, strains have been differentiated by comparing EC-GLC profiles. Metabolic products are affected by change in substrate. Media that can be reproduced from lot to lot are essential in some studies. The volatile components detected by EC-GLC in spent culture media consist mostly of bacterial metabolites, but the volatile compounds detected in body fluids may be bacterial metabolites, volatile components produced by the host in response to an infection, metabolites of cells associated with host defense, or a combination of two or more of these groups of compounds. The EC-GLC profiles obtained by analysis of synovial and cerebrospinal fluids appear to have good potential for use in diagnosing certain forms of arthritis and meningitis. Well-documented samples are essential to establishing EC-GLC profiles representative of a particular disease. A moderately priced computer would greatly aid in data processing and could be especially useful in compensating for minor changes in the retention times of peaks, which can occur as a result of column aging or when columns are renewed. An approach to the identification of components detected by EC-GLC, which makes use of electron capture gas chromatography-mass spectrometry, is presented.

Alcohols↗
PubMed1976

Study on the usefulness of hypertonic culture media.

Specimens from 300 patients were studied using five to nine aerobic and anaerobic culture media, including five that were hypertonic, Groups studied included fever of unknown origin, suspected endocarditis, endocarditis during therapy, bacteremia during therapy, abscess and cellulitis, presumed infectious arthritis, renal transplantation during rejection, collagen disease, sarcoidosis, lymphoma, and colitis. Isolates in hypertonic media were reverted to parent form by agar passage. In only 5% of these selected cases were organisms found in hypertonic, but not conventional, media that appeared on the basis of repeated isolation and/or serological studies to come from the patient. Nine of the 16 appeared to be of major significance. The two groups in which use of highly enriched, hypertonic media seemed most helpful were suspected endocarditis and undefined meningitis with negative cultures using standard media. The most effective of the hypertonic media used was 0.3 M sucrose in brain heart infusion with 20% horse serum. In most instances, the organism grew only in the hypertonic sucrose, and in most cases it appeared in conventional rather than aberrant form. Hypertonic media, especially 0.3 M sucrose, are of substantial helpin a small number of carefully selected cases.

Adolescent↗
PubMed1978

Quantitative evaluation of three commercial blood culture media for growth of anaerobic organisms.

The ability of three different commercial blood culture media--brain heart infusion broth (Pfizer), thiol broth (Difco), and PRS-peptone broth (Becton, Dickinson & Co.)--to support the growth of five different anaerobes is described. Inocula of 100 and 1,000 colony-forming units per ml were used to evaluate potential differences in survival, lag time, growth rate, and doubling times of each anaerobe in each medium. In addition, each medium was evaluated for its ability to neutralize the antibacterial effects of whole blood. The results of this study indicate that the PRS-peptone broth is superior to brain heart infusion and thiol broths. Shorter lag times and accelerated generation times and growth rates were noted for more different anaerobes in the PRS-peptone broth. Neither the size of inoculum nor the addition of normal whole blood appeared to alter the survival or growth characteristics of the anaerobes in any medium. However, the addition of normal whole blood did extend the lag time of each anaerobe by approximately 1 to 2 h in each medium.

Anaerobiosis↗
PubMed1979

Comparison of four culture media for isolation of Mycobacterium avium complex from porcine tissues.

The efficiency of four culture media was compared for the isolation of Mycobacterium avium complex from 197 procine tissues. In 82 tissues with microscopic granulomas and acid-fast bacilli, a significantly greater number of isolates were obtained on Middlebrook 7H10 medium with sodium pyruvate than on Stonebrink medium, Herrold egg yolk agar medium, or Lowenstein-Jensen medium (P=0.01). In 46 tissues in which no microscopic granulomas or acid-fast bacilli were observed, a significantly greater number of isolates were made on Middlebrook 7H10 medium or Herrold egg yolk agar medium than on Stonebrink medium or on Lowenstein-Jensen medium (P=0.01). The time required to grow M. avium complex on Lowenstein-Jensen medium was significantly greater than the time required to observe growth on Stonebrink, Middlebrook 7H10, or Herrold egg yolk agar medium (p=0.001).

Animals↗
PubMed1977

Immunoreactive luteinizing hormone, follicle stimulating hormone and their subunits in tissue culture media from normal and adenomatous, human pituitary fragments.

Fragments from 4 human pituitaries removed at surgery from one gonadectomized man and three women (one with Nelson's syndrome, one with Forbes Albright syndrome and one with chromophobe adenoma) were grown in tissue culture. The tissue culture medium was changed at weekly intervals, pooled and applied to a Sephadex G-100 column for gel filtration. In each eluate luteinizing hormone (LH), follicle stimulating hormone (FSH), alpha subunit and the beta subunits of LH and FSH were measured by specific radioimmunoassays. For comparison the pituitary standard LER 907 was similarly studied. LH and FSH were measurable in all studies. LH eluted over a broad area whereas FSH eluted as a much sharper peak. In all culture media and in LER 907 large quantities of alpha subunit were detected. The beta subunits of LH and FSH were not present in the LER 907 standard. LHbeta subunit was present in the culture medium of the pituitary fragments from the castrated man and from the women with Nelson's syndrome and Forbes Albright syndrome but not in that of the woman with chromophobe adenoma. FSHbeta subunit was detectable only in the latter case.

Adenoma, Chromophobe↗