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Parasites, diseases and health status of sympatric populations of sambar deer and white-tailed deer in Florida.

From December 1983 to December 1984 a study on parasites, diseases and health status was conducted on sympatric populations of sambar deer (Cervus unicolor) and white-tailed deer (Odocoileus virginianus) from St. Vincent Island, Franklin County, Florida. Ten sambar and six white-tailed deer were examined. White-tailed deer had antibodies to epizootic hemorrhagic disease virus and bluetongue virus. Serologic tests for antibodies to the etiologic agents of bovine virus diarrhea, infectious bovine rhinotracheitis, vesicular stomatitis, parainfluenza 3, brucellosis, and leptospirosis were negative in both species of deer. White-tailed deer harbored 19 species of parasites; all were typical of the parasite fauna of this species in coastal regions of the southeastern United States. Sambar deer harbored 13 species of parasites, which apparently were derived largely from white-tailed deer. The only exception was Dermacentor variabilis which occurs frequently on wild swine on the island. The general health status of sambar deer appeared to be better than that of white-tailed deer. This was hypothesized to result from the sambar deer's utilization of food resources unavailable or unacceptable to white-tailed deer and to the absence and/or lower frequency of certain pathogens in sambar deer.

Animals

Allozyme and mitochondrial DNA analysis of a hybrid zone between white-tailed deer and mule deer (Odocoileus) in west Texas.

Thirty allozyme loci and 35 mitochondrial DNA (mtDNA) restriction sites were examined in 24 white-tailed deer and 46 mule deer from a hybrid zone in West Texas. A common mtDNA genotype is shared by all of the mule deer with 67% of the white-tailed deer. At the albumin locus, 13% of the white-tailed deer and 24% of the mule deer are heterozygous, sharing alleles that are otherwise species-specific in allopatric populations; 7% of the mule deer are homozygous for the allele that is characteristic of allopatric white-tailed deer. Gene flow appears to have been bidirectional, with greater genetic introgression into mule deer. The mtDNA data suggest that matings between white-tailed and mule deer have occurred in the past. Despite evidence of genetic introgression, analysis of multilocus genotypes indicates that none of the deer examined is an F1 hybrid. Production of such hybrids appears to be generally uncommon in North American deer; management plans that assume otherwise should be reconsidered.

Alleles

A comparison of the seasonal hormone changes and patterns of growth, voluntary food intake and reproduction in juvenile and adult red deer (Cervus elaphus) and Père David's deer (Elaphurus davidianus) hinds.

Non-domesticated seasonally breeding ungulates exhibit marked seasonal changes in metabolic rate, voluntary food intake (VFI), pelage growth and moult and hormone secretion. It is not known whether these seasonal rhythms are regulated by the same central processes which control the onset and termination of the breeding season. Here we compare two closely related deer species which have significantly different mating and calving seasons. Seasonal changes in VFI, liveweight, coat growth, plasma prolactin and tri-iodothyronine (T3), and the timing of the breeding season were examined over a 15-month period in six adult post-pubertal red and Père David's deer from January to April the following year. The timing of the seasonal changes in prolactin, T3, VFI and coat growth were all significantly advanced by 56, 23, 60 and 54 days respectively in the Père David's deer. The times of onset and termination of the breeding season of Père David's deer were also significantly advanced by 90 days, but in both species, the breeding season was of similar duration (160 +/- 5 (S.E.M.) days). Changes in liveweight of adult red deer could be explained by changes in VFI rather than efficiency of utilization. This was not the case in Père David's deer and may indicate seasonal changes in the efficiency of energy utilization. In order to establish whether these species differences develop with age, we undertook a second study in which seasonal changes in VFI, growth, plasma prolactin concentrations and the timing of the onset of the breeding season were recorded for ten red deer and six Père David's deer from 6 to 18 months of age. Both species exhibited a similar decline in VFI in the first autumn of life. Subsequently, the Père David's deer exhibited an advance in the timing of the seasonal peak in VFI and prolactin (21 and 66 days respectively); puberty occurred 3 months earlier than in red deer. The earlier breeding season of the Père David's deer was associated with a significant advance in a range of seasonal endocrine and physiological parameters. These species differences may develop with age. Our data indicate that seasonal patterns of metabolism and growth may be closely linked to those mechanisms which also regulate the onset and termination of the breeding season.

Animals

In vitro fertilization of two species of deer mouse eggs by homologous or heterologous sperm and penetration of laboratory mouse eggs by deer mouse sperm.

Newly ovulated eggs from immature deer mice (Peromyscus maniculatus and P. polionotus) and mature laboratory mice (Mus musculus) treated with PMSG and HCG were inseminated in vitro with spermatozoa recovered from the cauda epididymidis of mature males. The time required for capacitation of deer mouse sperm in culture was estimated to be about two to five hours based on the dispersal of sperm agglutination and increase of sperm motility. The rate of sperm penetration through the zona pellucida of deer mouse eggs by homologous or heterologous sperm was relatively high (72-91%) but that of laboratory mouse eggs by deer mouse sperm was low (20-21%). After penetration through the zona pellucida, a high proportion of deer mouse eggs (79-93%) were fertilized by homologous or heterologous deer mouse sperm but no laboratory mouse eggs were fertilized by sperm of two species of deer mice. The zona pellucida was dissolved in a higher proportion of laboratory mouse eggs cultured with P. maniculatus (45%) than with P. polionotus sperm (3.4%), but this did not happen by incubation of deer mouse eggs with homologous or heterologous sperm. It seems that there is little difference in sperm penetration and fertilization between these two closely related species of deer mice but the reactions between the mouse eggs and deer mouse sperm are quite different.

Animals

Foot-and-mouth disease in British deer: transmission of virus to cattle, sheep and deer.

After exposure for two hours to cattle with foot-and-mouth disease, each of the five species of deer found in the British countryside became infected. Clinical disease was typical and severe in the roe and muntjac deer, with some animals dying, less severe in the sika deer and usually subclinical in the fallow and red deer. Each species transmitted disease to its own species and to cattle and sheep. The amounts of virus present in the blood, and in oesophageal/pharyngeal samples and excreted as an aerosol during the course of the infection in the deer were similar to those recorded for the sheep and cattle in the same experiment. The fallow and sika deer commonly carried virus in the pharynx beyond 28 days after exposure; some red deer also became carriers. In epidemics of foot-and-mouth disease in the UK, it is likely that deer would have such intimate contact with farm animals as occurred in this study. The natural behavior of free-living deer in the UK suggests that, although the five species are susceptible to foot-and-mouth disease, they are unlikely to be an important factor in the maintenance and transmission of the virus during an epidemic of foot-and-mouth disease in domestic livestock.

Air Microbiology

[Sarcosporidia of the cloven-hoofed game. III. Sarcosporidia in red deer and fallow deer].

The incidence of Sarcosporidia among red-deer and fallow-deer was investigated. The fallow-deer is less discussed because the low number of investigations. The red-deer shows with 15% the lowest incidence among the red-deer, fallow-deer and wild-boar investigated. The Sarcosporidia of the red-deer are smaller than those of the roe, therefore the identification in the trichinoscopic picture is more difficult. With regard to this it is recommended to name the Sarcosporidia of the red-deer Sarcocystis cervi (VON HESSLING, 1854), because their form is more similar to the cattle and not those of the roe. To be sure a greater number of investigations is necessary to detect all connections.

Animals

Spatial heterogeneity of mitochondrial DNA and allozymes among populations of white-tailed deer and mule deer.

A white-tailed deer (Odocoileus virginianus) population in northeastern Minnesota and a mule deer (O. hemionus) population in the Bridger Mountains Montana, have previously been shown to be spatially subdivided into contiguous subpopulations. We assessed the degree of genetic differentiation among subpopulations and tested the hypothesis that differentiation will be greater for mitochondrial DNA (mtDNA) than for nuclear-encoded allozymes. Differentiation of the white-tailed deer subpopulations was significant for two allozyme loci but not for mtDNA, and the overall degree of differentiation was low. Gene flow, recent founding of the subpopulations, and polygamous breeding structure may all have contributed to this pattern. Greater differentiation was evident among disjunct populations than between the contiguous subpopulations of white-tailed deer. The contiguous mule deer subpopulations were significantly differentiated for mtDNA and one allozyme locus. Differentiation was greater for mtDNA than for allozymes. These results are consistent with demographic data that indicate mule deer males disperse more than do females. Disjunct mule deer populations may be similar or dramatically different in mtDNA haplotype frequencies that do not necessarily vary with geographic distance. Current and historical gene flow and breeding structure will influence population genetic patterns.

Animals

Failure to detect infection in fallow deer (Cervus dama) exposed to Theileria cervi from white-tailed deer.

A frozen stabilate was produced from Theileria cervi sporozoites in salivary glands of adult Amblyomma americanum. The stabilate was inoculated into three fallow deer (Cervus dama) and two white-tailed deer (Odocoileus virginianus). Following inoculation, the white-tailed deer developed parasitemias as determined by blood smear examination at 11 and 13 days postexposure. Repeat examination of blood from the three fallow deer for 30 days postexposure failed to reveal observable piro-plasms. These findings indicate that fallow deer are not as susceptible to the Theileria cervi found in white-tailed deer from North America. Thus, there are some questions regarding the taxonomic position of this organism.

Animals

Nonlethal experimental inoculation of Columbia black-tailed deer (Odocoileus hemionus columbianus) with virus of epizootic hemorrhagic deer disease.

Intramuscular or intravenous inoculation of 5 Columbia black-tailed deer (Odocoileus hemionus columbianus) with virus of epizootic hemorrhagic deer disease (EHD) did not produce overt clinical disease. Two white-tailed deer (Odocoileus virginianus) exposed identically died in 5 to 6 days. There were no significant lesions in 1 black-tailed deer euthanatized on postinoculation day 5. The EHd virus was not isolated from the spleen of that deer. Seroconversion occurred in black-tailed deer, from zero EHD virus antibody titer before inoculation to titers of 1:128 to 1:256 after inoculation.

Animals

Immunolocalization of scrapie amyloid (PrP27-30) in chronic wasting disease of Rocky Mountain elk and hybrids of captive mule deer and white-tailed deer.

Scrapie amyloid-immunoreactive plaques are present in brain tissues of captive mule deer with chronic wasting disease (CWD), a progressive neurological disorder characterized neuropathologically by widespread spongiform change of the neuropil, intracytoplasmic vacuolation in neuronal perikarya and astrocytic hypertrophy and hyperplasia. We report here the immunolocalization of scrapie amyloid (PrP27-30) in plaques observed in brain tissues of Rocky Mountain elk (Cervus elaphus nelsoni) and hybrids of mule deer and white-tailed deer (Odocoileus virginianus) naturally affected with CWD. Similar findings have been shown in kuru, Creutzfeldt-Jakob disease, and Gerstmann-Sträussler syndrome in humans. Our data corroborate that CWD in Rocky Mountain elk and hybrids of mule deer and white-tailed deer belongs to the subacute spongiform virus encephalopathies (transmissible cerebral amyloidoses).

Animals

Studies on the blood of fallow deer (Dama dama) and red deer (Cervus elaphus): haematology, red cell enzymes, metabolic intermediates and glycolytic rates.

1. Blood samples were obtained from fallow deer (Dama dama) and red deer (Cervus elaphus). Basic haematology, red cell enzymes, and metabolic intermediates and the glycolytic rate of the red cells incubated with different substrates were measured. 2. The major findings were (i) the activity of glucose phosphate isomerase was notably high in the red blood cells of the red deer; (ii) red deer cells also utilized adenosine more efficiently than those of fallow deer and (iii) red cells of both species utilized galactose more efficiently than other species of ruminants.

Animals

[The supply of wild ruminants with major and trace elements. 2. The manganese content of winter grazing and the manganese status of red deer, fallow deer, roes and mouflons].

The winter grazing of wild ruminants on mantles of slate waste in the Harz mountains and in Gera county were richest in Mn whereas those on mantles of granite waste in the Erzgebirge were poorest. The flora of the shell-limestone, keuper and loess areas contains much less Mn than that of those acid habitats. The Mn-requirement of the wild ruminants grazing in the forests is met however, since bilberry plants (2,080 mg/kg), spruce twigs (984 mg/kg), spruce bark (827 mg/kg), oak twigs (791 mg/kg) and heather (754 mg/kg dry matter) in addition to many other plant species store extremely high amounts of Mn. Solely sallow twigs were poor in Mn (28 mg/kg). Based on 601 samples examined, the Mn supply of wild ruminants is extensively described. The rumen content reflected the plentiful Mn-supply of the wild ruminants living in forests (greater than 400 mg/kg) and the far worse one of field roes, particularly in Mn-deficiency areas for domesticated ruminants (mantle of shell-limestone waste 37 mg Mn/kg dry matter of rumen content). The indicator organs of the Mn-status (liver, covering hair, kidneys) verify the statements made concerning red deer, fallow deer and mouflons, of which a total of 170 head from 14 biotopes were examined. An Mn-deficit of field roes in Mn-deficiency habitats in winter cannot completely be excluded. Mouflons have not yet been able to adapt themselves to the excessive Mn-supply of the acid forest habitats in Central Europe. They stored significantly higher amounts of Mn in liver, covering hair, kidneys, cerebrum and ribs than the other wild ruminants and sheep and cattle. The normal Mn-content of the liver and the cerebrum of red deer, fallow deer and roes corresponds to that of sheep and cattle. Roes and fallow deer have winter covering hair poor in Mn (less than 4.0 mg/kg) in comparison to sheep and cattle.

Animal Nutritional Physiological Phenomena

[Investigations on the filariidae of the cervidae in Southern Germany. 3. The filariidae of roe deer (Capreolus capreolus) and fallow deer (Dama dama) (author's transl)].

Roe deer in southern Germany are frequently infected with W. rugosicauda. The adult worms are found subcutaneously in the connective tissues of the back of the host. The microfilariae concentrate in a considerable distance near the nose, in smaller numbers also in the ears and in the skin of the legs. The vector is probably Ixodes ricinus. In fallow deer C. wenki are found intradermally and subcutaneously. The microfilariae remain in the skin near the site of the adult worms. Skin inhabiting microfilariae of another filariid worm are found which cannot be associated with the described species of Cervidae. The host specificity of the filariid forms of roe deer, fallow deer and red deer is indicated, the development of the microfilarial distribution patterns is discussed.

Animals

[Clinical examination of the blood of roe deer (Capreolus capreolus L.) and fallow deer (Dama dama L.) naturally invaded by parasites].

Roe-deer (Capreolus capreolus L. -- five animals) and fallow deer (Dama dama L. -- eleven animals) of both sexes and at different age were subject to blood examination. The deer were killed in several preserves in Bohemia during autumn and winter shootings in two years. The following blood values were determined: erythrocyte count, leucocyte count, hemoglobin content, hematocrit values, MCV, MCH, MCHC, white blood picture, total protein, its fractions (albumin, alpha-, beta-, and gamma-globulin) SGOT, SGPT, and alkaline phosphatase activities, and calcium, phoshporus, and magnesium levels. Examinations were performed in the blood collected from heart soon after the killing of the animals and the results were evaluated in relation to natural polyvalent invasions by parasites of the following species: Bicaulus sagittatus, Dictyocaulus viviparus, Paramphistomum sp., Haemonchus contortus, Ostertagia circumcincta, O. ostertagi, Trichostrongylus colubriformis, Bunostomum trigonocephalum, Nematodirus filicollis, Chabertia ovina, Oesophagostomum columbianum, Trichocephalus ovis, Eimeria auburnensis, E. faurei, and E. ninaekohlyakimovae, occurring in different intensities and species composition in individual animals of the deer tested.

Alkaline Phosphatase

[Tuberculosis among Japanese deer in a deer-park in Goes (author's transl)].

Severe infection with tubercle bacilli of the avian type was detected among Japanese deer in Goes. All animals were disposed of and post-mortem studies were done, which showed that only two specimens were culturally negative. After thorough cleaning and decontamination of fecings and cages and burning of the mown grass during the period from August 1971 to April 1972, the park was repopulated with deer free from tuberculosis. The herd was continued to be free from tuberculosis ever since. Well over one hundred children who occasionally visted the deer-park as well as the attendants of the deer were examined twice (at a four-month interval) using avian tuberculin, the results of the tests being negative.

Animals

Reinvestigation of the primary structures of red deer and roe deer pancreatic ribonuclease and proline sites in mammalian ribonucleases.

The sequences of amino acid residues 15-23 of red deer (Cervus elaphus) and roe deer (Capreolus capreolus) pancreatic ribonuclease and the identity of residue 99 in roe deer ribonuclease are corrected. Earlier results are explained by the cleavage of an Asp-Pro bond in both enzymes during the treatment with CNBr in 70% formic acid and by wrong interpretations of amino acid analyses. Proline residues, which occur at a number of positions in several mammalian ribonucleases, can be accommodated in a model of bovine ribonuclease S without disrupting the conformation of the main chain.

Amino Acid Sequence

Fertility in an F1 male hybrid of white-tailed deer (Odocoileus virginianus) x mule deer (O. hemionus).

The fecundity of an F1 male hybrid deer, from a cross between a male Odocoileus virginianus (white-tailed deer) and a female O. hemionus (mule deer), was assessed by cytogenetic and flow cytometric techniques. Analysis of chromosome morphology, nucleolus organizer expression, meiotic chromosome pairing, sperm production, and nuclear gene inheritance revealed no genetic anomalies that could potentially impair normal fertility. These observations are discussed in relation to recent reports of hybridization between natural populations of these two species.

Albumins

The pineal gland of the red deer male. Histology and ultrastructure with notes on the fallow deer and mouflon pineals.

The most conspicuous morphological feature of the investigated red deer pineal gland was very deep pineal recess, covered with continuous layer of the ependymal cells. Its branches (2-3) reached the distal part of the gland. The slight differences in pineal structure of sexually active and sexually inactive red deer were observed. Relatively large pineal glands both of mouflon and fallow deer showed many common as well as different structural features.

Animals