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Objective quantification method for measuring in vivo accumulated dental plaque.

Dental plaque was collected, dried, and weighed on a gold insert that fit into the buccal aspect of a posterior crown. The plaque weight was determined by calculating the difference between the postcollection insert weight and the precollection insert weight. This system for measurement of the dry weight of in vivo accumulated dental plaque was found to be a practical means of plaque quantification.

Adult

Correlation between streptococci of human dental plaques and dental caries.

Plaque samples from 27 buccal tooth surfaces were obtained with sterile McCall curettes from available third molars. Bacteriological sampling and biochemical tests were carried out to identify four groups of streptococci, i.e. Str. mutans, Str. spp., Str. sanguis and Str. mitis. Clinical examinations carried out 6 months after the dental scrapings were taken showed a strong positive correlation between detectable lesions and Str. mutans.

Dental Caries

[Occurence of dental plaque among Norwegian children with systematic school dental care. A method for identification of patients with special need for dental plaque control].

The prevalence of dental plaque was assessed in 1534 school children 7-12 years old in Porsgrunn, Norway. Only the first molars and the incisors were examined. In each child 48 scorings were carried out by the Plaque Index (Pl I) (Silness & Löe 1964). The frequencies of Pl I score 0, 1 and 2 were calculated per individual, school class and school. Score 0 expresses an optimal situation whereas score 2 is assumed to indicate the need for treatment. These features have been visualized, and the distribution pattern seems to be suitable for dental public health workers. Mean Pl I was 1.50. Score 3 was not observed. 55% of the tooth surfaces was covered with dental plaque corresponding to score 2, whereas score 1 was observed in 40%. Children with score 0 on all tooth surfaces were not registered. Great variation in plaque prevalence was found between the school classes. The 6. grade (aged 12 years) children showed the best condition.

Child

Xylitol binding in human dental plaque.

Human dental plaque and whole saliva sediment were tested for their ability to bind 14C-labeled sucrose, fructose, glucose, sorbitol, and xylitol. Sucrose, glucose, sorbitol, and fructose were bound to all materials tested, in this decreasing order. The binding was strongest with plaque given a five-second ultrasonic shock, and lowest with salivary sediment. Xylitol was only insignificantly bound, indicating that plaque microorganisms possess specific recognition sites for xylitol to a very restricted extent.

Adult

Experimental gingivitis in ODU plaque-susceptible rats. III. Toxic activity of the rat dental plaque.

The toxicity tests of the dental plaque from ODU plaque-susceptible rats showed strong lethal effect on mice, and abscess forming effect on guinea-pigs. Bacterial cells isolated from the rat dental plaque also showed strong toxicity on both animals and capillary permeable activity on rabbits. Among these bacterial cells, Corynebacterium showed the strongest toxic effects on these animals. These facts suggested an important role of the dental plaque on initiation and development of gingivitis and that especially Corynebacterium may play an important role on gingivitis in ODU plaque-susceptible rats.

Animals

Degradation of maltose and starch by human saliva and by supernatants of dental plaque material.

The pH-decrease in dental plaque in vivo after mouth rinses with solutions of sucrose and maltose has been compared. No statistically significant differences were found between the results obtained with these two disaccharides. Pooled samples of parotid saliva, centrifuged whole saliva and dental plaque supernatants were separated with the isoelectric focusing technique. The results suggest that maltose is hydrolysed in parotid saliva by the salivary alpha-amylase and in dental plaque by enzymes from sources other than the salivary glands, probably microorganisms. No bacterial alpha-amylases, separated from salivary alpha-amylase, were found in whole saliva specimens or in dental plaque supernatants. Quantitative determinations of "maltase" and amylase activities in parotid saliva, centrifuged whole saliva and dental plaque supernatants from 10 subjects were carried out. The highest enzyme activity of "maltase" was found in the samples of dental plaque and the highest amylase activity in parotid saliva specimens.

Amylases

Studies on human dental plaque. 1. Physical and chemical characteristics and enzyme activities of pooled plaque extracts.

Human supragingival dental plaque was collected from patients with various degrees of caries and periodontal disease. Plaque extracts, prepared in five different solutions (four varied from pH 1.8 to 12.7; one contained urea), were analyzed by polyacrylamide gel electrophoresis, and tested for amylase and lysozyme enzyme activity. Because no qualitative or quantitative advantages of using the extremes of pH or urea were observed, all subsequent extracts were prepared in phosphate buffered saline at pH 7.3. Concentrated extracts were fractionated by gel filtration and characterized by polyacrylamide gel electrophoresis, peptide mapping, molecular weight estimation, determination of enzymatic activities and amino acid and carbohydrate analyses. Regions of similarity among the gels were revealed by comparing the electrophoretic patterns of pooled plaque extract, normal serum and whole saliva. The elution pattern of pooled plaque extract from a standardized Sephadex G-200 column indicated the presence of both high and low molecular weight proteins that might have correlated with the components of normal serum and saliva. A predominant and dialyzable third fraction had no correlate in either serum or saliva. The small peptides in this fraction were subjected to amino acid, carbohydrate and peptide map analyses. The most abundant amino acids were alanine, glutamic acid, glycine, valine, leucine, lysine and serine. These small components contained no neutral or amino sugars. Pooled plaque extract and the small peptides exhibited similar peptide maps.

Alanine

The effect of instruction and supervised toothbrushing on the reduction of dental plaque in kindergarten children.

Based upon the present study the following conclusions were reached: Supervised toothbrushing with instruction for preschool children significantly and consistently decreased dental plaque scores, when compared to the control group of the same children who had only supervision. While those subjects receiving toothbrushing instructions and supervision had significant plaque reductions, at no time did the plaque scores reach zero. A symmetrical but nonuniform distribution of dental plaque prior to toothbrushing was noted as follows: Posterior teeth had higher dental plaque scores than anterior teeth. More dental plaque was present on the buccal surfaces of the maxillary teeth than on the lingual surfaces. More dental plaque was present on the lingual surfaces of the mandibular teeth than on the buccal surfaces. The distribution of dental plaque following toothbrushing remained the same as the prebrushing pattern, but the amount of dental plaque was reduced by 31 percent. Constant reinforcement is necessary to maintain effective plaque control in pre-school children.

Child Behavior