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At least 19 recordsLinked to original sources
PubMed1976

Enzymatic destruction of the elastic lamella at the mouth of cerebral berry aneurysm? An ultrastructural study with special regard to the elastic tissue.

Destruction of the elastic tissue is probably an acquired lesion and is decisive for the appearance of cerebral arterial aneurysms at the sites of congenital media defects. The elastic component in the mouths of aneurysms has therefore been studied by electron microscopy, using two new staining methods, i.e. ruthenium-red staining and prolonged osmium-tetroxide treatment. The hypertrophic, duplicated, elastic lamellae showed a disintegration of their luminal portions not earlier described. In close connection with the disintegrated portions, extracellular lysosome-like granules were observed. It is hypothesized that discharged leucocyte grannules containing elastase help to destroy the elastic lamellae.

Adult↗
PubMedDate not supplied

An electron microscopic technique for the study of elastic tissue in small arteries and arterioles of the kidney.

Elastic tissue content and distribution were studied by electron microscopy of small arteries and arterioles of kidneys obtained from normotensive Wistar and spontaneously hypertensive rats, normal dogs and patients with hypertension and glomerular diseases. Specificity and sensitivity of silver tetraphenyl porphyrin sulfonate stain are discussed. This specific electron microscopy technique should prove useful in analysis of elastic tissue in pathologic states of other organs, especially where elastic tissue normally appears to be sparse or absent.

Animals↗
PubMedDate not supplied

Effect of thiouracil upon canine arterial elastic tissue.

Unlike some other mammalian species, the dog is relatively resistant to the development of elevated levels of serum cholesterol after prolonged cholesterol feeding. This may be overcome by suppressing thyroid activity with thiouracil. Information regarding possible activity of thiouracil itself upon the arterial tissues is almost nonexistent. The present investigation was undertaken to test whether this drug has any such action, especially upon the arterial elastic tissues. Destructive changes were observed in arterial elastic tissues in dogs given thiouracil for three and six months. The changes consisted of accentuation of the elastic fibrillar components, formation and subsequent coalescence of clefts, and fragmentation and ultimate "dissolution" of the elastic elements. The results suggest that thiouracil may exert a damaging effect upon the arterial elastic fibers; thus, it is possible that one of the mechanisms by which thiouracil and cholesterol administration induces experimental atherosclerosis in the dog is by elastic tissue destruction, possibly promoting the subsequent lipid accumulation in the arterial wall.

Animals↗
PubMed1979

The histomorphology of the elastic tissue system in the skin of the human hand.

This study compares the effectiveness of the Verhoeff and Puchtler-Sweat resorcin fuschin methods of staining the elastic tissue of palmar and dorsal skin specimens of the human hand. The resorcin stain demonstrates the "oxytalan" fibres of the elastic tissue system in the dermal papillary layer of the palmar skin. Oxytalan fibres are not generally demonstrated by the Verhoeff method and are not demonstrated in the dorsal skin specimens. These studies substantiate the existence of a system of elastic tissue fibres in the skin of the hand. The inter-relationship of the elastic and collagenous fibres appears to complement each of their functions; namely, in anchoring the dermo-epidermal junction, absorbing the stresses of stretch and compressive forces and return the tissues to their original state of tension, and providing protection to the dermal appendages in addition to lending a gradient mobility transmitted through the dermis to the subcutaneous layer to give suppleness and mobility to the skin.

Adult↗
PubMed1976

Iron gallein elastic method---a substitute for Verhoeff's elastic tissue stain.

A method for staining elastic fibers in formalin fixed, paraffin embedded sections is described. After deparaffinizing and dehydration, sections are stained for 30 minutes in a solution prepared by mixing equal parts of 1% gallein dissolved in ethylene glycol and absolute alcohol (1:4), and 1.16% aqueous ferric chloride in 1% hydrochloric acid. The sections are washed in water and then differentiated in 2% ferric chloride for 2 minutes. After washing in water, the sections are counterstained with a variant of Van Gieson's picric acid-acid fuchsin for 1 minute. The results are similar to Verhoeff's elastic stain with elastic fibers staining black. An advantage to this staining procedure is that visually controlled differentiation is not necessary.

Aorta↗
PubMed1976

An evaluation of elastic tissue staining.

The Special Procedures Laboratory of the Department of Pathology at M.D. Anderson Hospital was concerned with the nationwide shortage of hematoxylin. The amount of this dye required for various staining solutions was calculated to determine thrifty usage. Some solutions required more hematoxylin than was available and prompted the serious investigation of existing techniques and new methods for routine laboratory use. Evaluation of various techniques resulted in a significantly successful modification of Gomori's aldehyde fuchsin technique, which was subsequently adopted as a routine procedure for elastic tissue staining in the Special Procedures Laboratory.

Aldehydes↗
PubMed1975

A methanol resorcin-fuchsin stain for elastic tissues and nuclei.

The staining properties of conventional ethanol resorcin-fuchsin and of methanol resorcin-fuchsin were compared. Formula; Dissolve 0.2 g of commercial resorcin-fuschin in 70 ml of methanol or ethanol, add 30 ml of water and 1 m1 of concentrated HC1; stain sections for 4 hours. Both solutions colored elastic and pseudoelastic fibers, cartilage and some mucins. Methanol resorcin-fuchsin also colored nuclei in methacarn- (methanol-chloroform-glacial acetic acid 6:3:1) and formalin-fixed tissues; this nuclear stain withstood counterstaining with picro-dye mictures. Zenker-fixed sections showed diffuse coloration with little or no contrast between nuclei and cytoplasm. Extraction with hot trichloracetic acid abolished binding of methylene blue, but binding of methanol resorcin-fuchsin by nuclei remained unaltered or was enhanced. Experiments with solvents containing various concentrations of methanol, ethanol or isopropanol indicated that the staining patterns of resorcin-fuchsin are determined by the nature and concentration of the alcohol. Methanol resorcin-fuchsin proved useful for simultaneous visualization of elastic tissues and nuclei.

Elastic Tissue↗
PubMed1977

Preparation of a specific antiserum towards the microfibrillar protein of elastic tissues.

The microfibrillar protein (MFP) of bovine foetal ligamentum nuchae was prepared and used to induce an antiserum (I) in rabbit. Antibody (I), reacted with a dispersion of MFP in Ouchterlony plates, produced one major precipitin band (A) and two minor bands (B and C). The major band (A) was used to prepare antiserum (II) in a second rabbit. Antibody (II) was monospecific for precipitin band (A) and was shown to be selectively directed against MFP of bovine and chick elastic fibres by immunofluorescent and immunoperoxidase techniques. Immunoperoxidase-labelling techniques demonstrated the regular organization of microfibrillar protein on the surface of elastic fibres and suggested the ability of MFP to assemble into a framework defining the three dimensional polymerization of elastin monomers during the formation of elastin fibres. It was observed that collagen fibrils, which were in close association with the microfibrillar protein on the surface of elastic fibres, also possessed a protein which was immunologically related to MFP.

Animals↗
PubMed1977

Immunofluorescence studies with a specific antiserum to the microfibrillar protein of elastic fibres. Location in elastic and non-elastic connective tissues.

The microfibrillar protein (MFP) of foetal bovine ligamentum nuchae elastic fibres was prepared and used to produce a monospecific antiserum. Indirect immunofluorescence studies employing the specific antiserum demonstrated its selectivity for MFP associated with elastic fibres. The antiserum was demonstrated to react with vascular elastic tissue, perivascular connective tissue and reticular basement membranes in a variety of tissues. This evidence suggests that the MFP (or an immunologically related protein) is not confined to elastic fibres but is widely distributed in connective tissue being associated with both elastin and collagen fibres.

Animals↗
PubMed1979

A comparative light microscopic evaluation of oxytalan fiber staining with a variety of dye substances.

Staining of oxytalan fibers in marsupial, eutherian and human periodontal ligaments was surveyed with 65 different dyes. Using the criteria of response to preoxidation, distribution, and morphologic appearance, 27 dye preparations in addition to the Gomori aldehyde-fuchsin, Taenzer-Unna orcein, and Weigert resorcin-fuchsin techniques displayed oxytalan fibers. With two exceptions all dyes were cationic and reacted with varying degrees of excellence with different animals. Most dyes produced their best staining results as concentrated solutions in 3% acetic acid, suggesting involvement of oxidatively engendered polyanions predominantly associated with an acid mucopolysaccharide component of the oxytalan fiber. The significance of carboxyl and sulfur-containing groups should not be overlooked in further studies aiming to elucidate oxytalan fiber chemistry and microstructure. This study supported the view that oxytalan fibers belong to the family of elastic tissues and represent a biologically important system within the periodontal ligament.

Animals↗
PubMed1977

[Chronic lathyrism. Protective effect of pyridinol carbamate on connective tissue lesions in the rat (author's transl)].

The use of lathyric toxins (BAPN) in low dosage (1 g/kg/day) for a period of 8 weeks caused in the rat simultaneous changes in the skin and aortic connective tissues. In the skin. collagen tissue was dislocated and broken in fragments, the elastic tissue disappeared, the fibroblasts were vacuolized and presented evidence of injury. These lesions were comparable to those observed in human skin during ageing. The addition of pyridinol carbamate (PDC) to BAPN prevents the formation of lesions of the elastic tissue and of fibroblasts. When given after the cessation of the lathyrogen treatment PDC arrested the formation of the lesions and accelerated their regression.

Aminopropionitrile↗
PubMed1976

Ultrastructure of elastosis in scirrhous carcinoma of the breast.

The stroma of scirrhous breast carcinoma frequently contains an abundance of material with the histologic staining properties of elastic tissue referred to as elastosis. In the present study, this lesion was found to correspond at the ultrastructural level to elastic fibers of various sizes and random orientation. These fibers displayed the two characteristic components, consisting of an amorphous core with a peripheral mantle of microfibrils. Interspersed among the elastic fibers were modified fibroblasts. These cells exhibited an irregular contour with numerous cytoplasmic processes and indentations. Their cytoplasm contained abundant rough endoplasmic reticulum, prominent Golgi complexes, and bundles of filaments. In the extracellular space, elastic fibers were often closely apposed to the plasma membrane of the fibroblasts. Carcinoma cells were also observed in the elastic tissue. It is postulated that fibroblasts, probably under an inductive influence from cancer cells, are the cells responsible for elastic production.

Adenocarcinoma, Scirrhous↗
PubMed1975

Ultrastructural changes in scleromyxedema.

Skin biopsy specimens from a 60-year-old patient with paraproteinemia and generalized changes of the skin typical of scleromyxedema were studied with the electron microscope. The dermis was dominated by collagen fibrils and accumulations of peculiar connective tissue cells, while elastic tissue was sparse and in some areas completely absent. Large, sharply demarcated areas of a filamentous material were occasionally observed. The collagen fibrils were often surrounded by thin filaments with a periodic segmentation and by many glycosaminoglycan (mucopolysaccharide) filaments. The elastic fibres contained large amounts of elastic fibrils and small amounts of a homogenous matrix. The cytoplasm of the above-mentioned cells was dominated by lysosomes in different stages of development, often occupying almost all the cytoplasmic area. The collagen fibrils were found in close relation to these cells, frequently inside invaginations of the cells. Furthermore, collagen fibrils were observed free in the cytoplasmic area and inside the lysosomes, indicating lysosomal degradation of collagen.

Cell Membrane↗