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The value of embryo transfer to cattle breeding in Britain.

An analysis is made of the maximum expenditure which could be justified in embryo transfer in cattle is used to: increase the rate of genetic improvement of dairy or beef cattle; increase the frequency of twin-pregnancies; and expedite a change of breed. Estimates of maximum justifiable expenditure have been compared with an estimate of the cost of non-surgical transfer. Embryo transfer should be used in elite beef herds to increase selection intensity, particularly if bulls from such herds can be used for artificial insemination. Other commercial applications will not be economically justifiable until the cost of transfer has fallen by 50 to 80 per cent.

Animals

A simple technique for nonsurgical embryo transfer in mice.

A simple method was developed for nonsurgical transfer of mouse embryos which enabled transfer to both uterine horns. Embryos were picked up in a modified capillary tube and transferred through the cervix into each uterine horn of an unanesthetized mouse. A glass speculum was used to facilitate location of the cervix. The technique was found to be as successful (up to 60% of embryos transferred developed to term) as surgical methods yet was simpler and eliminated surgical trauma to the recipient mouse.

Animals

Embryo transfer for cows with reproductive problems.

Surgical embryo recovery and transfer methods were applied to 25 cows and heifers with chronic reproductive problems. In 16 of the 25 animals, abnormalities could not be detected by palpation per rectum and, at laparotomy, the probable cause of low fertility was found to be oviductal obstructions or periovarian adhesions in 6 of them. Four pregnancies were obtained from 3 of these 6 animals. A definitive diagnosis was not obtained through laparotomy in the other 10 animals in this group; however, an abnormal uterine environment associated with senescence may have been responsible for low fertility in the older cows. Eighteen calves were obtained by embryo transfer from 8 of these 10 donor animals. The remaining 9 heifers and cows had lesions detectable by palpation per rectum. Chronic, purulent metritis was found in 2 cows, one of which produced 7 calves by embryo transfer. The other 7 had periovarian adhesions, and 8 calves were obtained from 2 of them. The adhesions in 6 of these 7 cows were attributable to cesarean section. Laparotomy was valuable for defining the extent of the adhesions and establishing a reliable prognosis in some cases.

Animals

Embryo transfer in pigs: a method for introducing genetic material into primary specific-pathogen-free herds.

Fifty-five embryos were transferred from 4 Duroc donor sows into 4 crossbred recipient gilts. The embryo survival rate was 66%; 53% of eggs ovulated by donors were represented by live pigs at term. Three of the litters were obtained by hysterectomy of the recipients and introduced into a primary specific-pathogen-free herd, thereby saving the donor sows for subsequent use. Embryo transfer provided a method for exploiting the genetic potential of superior females.

Animals

Embryo transfer in cattle: an evaluation of the current situation.

A technique for the transcervical recovery of ova in cattle is described. The donors recipient pool consisted of 122 cows of different ages and 4 different breeds. Of these 102 cows were superovulated. For recovery of ova an apparatus consisting of a metal catheter glued to the inside of a 3-way Foley catheter was used. The technique used for embryo transfer was basically the same as for collecting the embryos. The 102 donors yielded at slaughter a total of 956 corpora lutea i.e. an average of 9,4 per cow. Of the ova released 294 or 30% were recovered on transcervical flushing of the uterus. 40% of the inovulated cows conceived. The future development of inovulation in cattle is discussed on the basis of the results recorded and on those previously documented.

Animals