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At least 19 recordsLinked to original sources
PubMed2000

Dietary marine fatty acids (fish oil) for asthma.

BACKGROUND: Epidemiological studies suggest that a diet high in marine fatty acids (fish oil) may have beneficial effects on inflammatory conditions such as rheumatoid arthritis and possibly asthma. OBJECTIVES: 1. To determine the effect of marine n-3 fatty acid (fish oil) supplementation in asthma. 2. To determine the effect of a diet high in fish oil in asthma. SEARCH STRATEGY: The Cochrane Airways Review Group register was search using the terms: marine fatty acids OR diet OR nutrition OR fish oil OR eicosapentaenoic acid OR EPA. Bibliographies of retrieved trials were searched and fish oil manufacturers contacted. SELECTION CRITERIA: Randomised controlled trials in patients with asthma more than two years of age were included. The study duration had to be in excess of 4 weeks. Double blind trials were preferred, but single-blind and open trials were also reviewed for possible inclusion. Three reviewers read each paper, blind to its identity. Decisions concerning inclusion were made by simple majority. Quality assessment was performed by all three reviewers independently. DATA COLLECTION AND ANALYSIS: The only comparison possible was between marine n-3 fatty acid supplementation and placebo. There were insufficient trials to examine dietary manipulation alone. MAIN RESULTS: Eight randomised controlled trials conducted between 1986 and 1998 satisfied the inclusion criteria. Six were of parallel design and two were cross-over studies. Seven compared fish oil with placebo whilst one compared high dose vs low dose marine n-3 fatty acid supplementation. None of the included studies reported asthma exacerbations, health status or hospital admissions. There was no consistent effect on any of the analyzable outcomes: FEV1, peak flow rate, asthma symptoms, asthma medication use or bronchial hyper reactivity. The single study performed in children also combined dietary manipulation with fish oil supplementation and showed improved peak flow and reduced asthma medication use. There were no adverse events associated with fish oil supplements. REVIEWER'S CONCLUSIONS: There is little evidence to recommend that people with asthma supplement or modify their dietary intake of marine n-3 fatty acids (fish oil) in order to improve their asthma control. Equally, there is no evidence that they are at risk if they do so.

Adult↗
PubMed2002

Influence of fish consumption on the distribution of serum cholesterol in lipoprotein fractions: comparative study among fish-consuming and non-fish-consuming populations.

The objective of this study was to investigate serum lipoprotein levels in order to assess cardiovascular disease (CVD) risk factors between fish-consuming populations and non-fish-consuming populations, as it has been speculated that fish intake reduces CVD risk. A representative sample of one thousand subjects (529 men and 471 women) were selected, with ages ranging from 20 to 70 years, from 40 villages belonging to fish-consuming (500) or non-fish-consuming (500) populations. Serum lipoprotein lipids such as total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C) were analysed biochemically using standard procedures. The ratios of TC: HDL-C and LDL-C: HDL-C were computed. Mean values of serum LDL-C and the ratios of LDL-C: HDL-C and TC: HDL-C were significantly lower and HDL-C levels were higher in fish-consumers than in non-fish-consumers. The concentrations of HDL-C decreased with increasing age, while the reverse was true for LDL-C and for the LDL-C: HDL-C and TC: HDL-C ratios. There were significant sex differences for certain age groups in both of the population groups. The 5th, 50th and 95th percentile cut-off values for these parameters were lower in fish-consumers than in non-fish-consumers. The prevalence of individuals at risk of CVD because of low HDL-C (<35 mg/dL), high LDL-C (>130 mg/dL) and their atherogenic ratios (LDL-C: HDL-C >3.5 and TC: HDL-C>4.5) was significantly greater in non-fish-consumers. This study highlights that the fish-consuming population had a lower atherogenic risk than the non-fish-consuming population. The intake of fish may have substantial implications for public health and health economy by decreasing the risk of CVD. However, more studies are warranted to better define the mechanisms of cardioprotection by dietary fish and fish oils.

Adult↗
PubMed1999

Fish allergy: is cross-reactivity among fish species relevant? Double-blind placebo-controlled food challenge studies of fish allergic adults.

BACKGROUND: Allergic reactions to fish are a common cause of food allergy in many areas of the world where fish is a major source of protein. Although different species of fish may be consumed, possible cross-reactivity has received limited investigation. OBJECTIVE: The aim of this study was to assess potential cross-reactivity to different species of fish species using double-blind, placebo-controlled food challenges (DBPCFC) in fish-allergic adults and to compare skin test and RAST reactivity with the challenge response. METHODS: Nine skin prick test and/or RAST-positive adult individuals with histories of an immediate-type reaction following fish ingestion were challenged with different fish species using double-blind, placebo-controlled food challenge. RESULTS: Of a total of 19 double-blind, placebo-controlled fish challenges performed, 14 challenges (74%) resulted in the induction of objective signs that were consistent with an IgE-mediated response. The most common sign observed was emesis (37%); the most prevalent subjective symptoms reported were compatible with the oral allergy syndrome (84%). Three subjects reacted to at least three fish species and one subject reacted to two fish species tested. In regard to the positive challenges, predictive accuracy of skin prick test and RAST was 84% and 78%, respectively. CONCLUSION: Our results indicate that clinically relevant cross-reactivity among various species of fish may exist. Advising fish-allergic subjects to avoid all fish species should be emphasized until a species can be proven safe to eat by provocative challenge.

Adult↗
PubMed1985

Studies on fish protein concentrate and fish meal from river Nile bolti fish (Tilapia nilotica).

Fish protein concentrate (FPC) from river Nile bolti fish (Tilapia nilotica) was prepared and compared with commercial FPC and fresh bolti fish flesh. Fish meal (FM) from bolti fish offals was prepared and compared with commercial FM and also fresh bolti fish flesh. FPC from bolti fish showed a higher crude protein content but less fat, ash, calcium and sand than the commercial sample, while FM from bolti fish showed a higher content of ash and phosphorus than commercial FM but was nearly similar in crude protein, fat, calcium and sodium chloride. FPC from bolti fish had a higher content of lysine, arginine, aspartic acid, glycine and glutamic acid and a lower content of the other free amino acids. The bolti fish FM had a lower content of total amino acids and the contents of the free amino acids cysteine, glycine, aspartic acid, serine, alanine, valine, and methionine increased slightly. The yield was 12% for FPC and 19.5% for FM. Coliform bacteria were not present in both FPC and FM from bolti fish. Low moisture contents of FPC and FM were essential for preventing microbiol growth and to attain a good keeping quality. The FPC and FM from bolti fish reached moisture equilibrium and stopped increasing in weight within 144 to 192 h.

Amino Acids↗
PubMed2000

Fish intake and blood lipids in fish eating vs non-fish eating communities of coastal south India.

The lower blood lipid levels and lower incidence of coronary heart disease in fish eating populations has been attributed to the unusual n-3 fatty acids present in their fish diet. In order to test this hypothesis, a cross-sectional study was proposed to evaluate the lipid profile in free-living men and women of fish eating and non-fish eating populations aged between 20 to 70 years in coastal villages of the district of Nellore, in south India. The mean concentrations of total serum cholesterol and triacylglycerols were found to be lower in fish eating than in non-fish eating populations. Significant differences were observed between populations for certain age groups. The serum phospholipid content was lower in fish-eating than non-fish eating populations and the difference was found to be significant only for women. The mean total cholesterol and triacylglycerol levels were found elevated consistently as the age advanced in both sexes and populations. There were significant differences for blood lipids between men and women in some age groups. The percentile cut-off values for cholesterol and triacylglycerol were lower in fish eating as compared to non-fish eating group. Prevalence of hypercholesterolemia and hypertriglyceridemia (> or = 200 mg/dl) was significantly lower in fish eating people indicating less proneness to cardiovascular risk. The data indicate that eating of fish may have a role in ameliorating the risk of coronary heart disease.

Adult↗
PubMed1998

Levels of toxaphene indicator compounds in fish meal, fish oil and fish feed.

Three toxaphene indicator compounds were determined in fish oil and fish meal of different origin and raw material as well as in feed containing fish oil and fish meal. Fish oil and fish meal from South America contained no or only traces of the indicator congeners. The sum of the three compounds varied in fish oils from Europe between 13 micrograms/kg fat (sand eel oil) and 206 micrograms/kg fat (cod oil). Varying concentrations were also determined in European fish meal and in feed. Toxaphene indicator compounds were as well detected in trout and salmon fed with toxaphene residues containing feed. Results indicate that feed can contribute to toxaphene contamination of farmed salmon from Europe.

Animal Feed↗
PubMed1996

Fishing in a polluted estuary: fishing behavior, fish consumption, and potential risk.

People make subjective judgments about hazards relying on what they know and feel. These risk perceptions may be based on accurate or inaccurate information and are often optimistically biased. The existence of uncertainties in the evaluation of many environmental hazards effects how risks are perceived. This paper examines fish consumption and risk perception of urban fishermen in the New York/New Jersey estuary, in areas where there were consumption advisories. We interviewed 318 fishermen and crabbers in the Arthur Kill, Raritan Bay, and New Jersey shore. Fish were eaten an average of at least four times per month in all regions, but fishermen in the Arthur Kill fished most frequently, averaging over eight times per month. Although 60% of fishermen and crabbers in the Arthur Kill reported hearing warnings about consuming fish caught in these waters, 70% of fishermen and 76% of crabbers said they are their catch. Significantly fewer fishermen in the Bay and Shore regions had heard warnings (28% and 30%, respectively), and more reported consuming their catch (88% and 82%, respectively). In all regions, most people thought that the fish were safe to eat, many believing they were "fresher" than store bought fish. Thus, most people ignored the consumption advisories in effect for these waters. Some of these people are consuming high quantities of fish and crabs, and thus are exposed to potentially deleterious levels of contaminants. In general, people failed to consider the possibility of chronic effects and did not perceive that this enjoyable, familiar pastime could be hazardous. Further, fishermen generally had great confidence in their own knowledge, which proved to be inaccurate in many cases, and often expressed distrust in the information source (government). Clearly, simply issuing consumption advisories is insufficient to promote risk-reducing behavior.

Animals↗
PubMed2002

Mutagencity and antimutagencity studies of lipidic extracts from yellowtail fish (Seriola lalandi), lisa fish (Mugil cephalus) and cazón fish (Mustelus lunulatus).

Organic extracts from fresh and smoked yellowtail fish (Seriola lalandi), lisa fish (Mugil cephalus) and cazon fish (Mustelus lunulatus) were tested for mutagenicity using Ames Salmonella tester strains TA98 and TA100 with metabolic activation (S9). Also, the antimutagenicity of the organic extract from yellowtail fish was tested against aflatoxin B1 (AFB1). Yellowtail fish extract was sequentially fractionated by thin-layer chromatography (TLC) and each fraction was also tested for antimutagenicity. None of the fresh species showed mutagenicity. Extract from smoked yellowtail showed the highest mutagenic potential among the smoked species tested. Organic extract from fresh yellowtail reduced the number of revertants caused by AFB1 showing a dose response type of relationship. Sequential TLC fractionation of the antimutagenic extract produced four antimutagenic fractions from fresh yellowtail fish. These results that the lipidic fraction of the species tested contains at least four compounds with chemoprotective properties that reduce the mutagenicity of AFB1.

Aflatoxin B1↗
PubMed1996

Interaction between fish spoilage bacteria Pseudomonas sp. and Shewanella putrefaciens in fish extracts and on fish tissue.

The interaction between fish spoilage bacteria, Pseudomonas sp. and Shewanella putrefaciens, was investigated using fish extract and fish tissue as model systems. Isolates of Pseudomonas that produced iron chelators, siderophores, inhibited growth of S. putrefaciens in a fish-extract-agar diffusion assay but no, or only weak, antagonistic activity was seen when the medium was supplemented with iron. Sterile-filtered supernatant fluid from a siderophore-producing Pseudomonas grown in fish extract was inhibitory to S. putrefaciens if the number of Psudomonas was above 10(8) cfu ml-1. In contrast, supernatant fluids from siderophore-negative Pseudomonas isolates did not inhibit growth of S. putrefaciens. The inhibitory effect was, except for one strain of Pseudomonas, not seen in supernatant fluids from iron-enriched cultures of Pseudomonas sp. Finally, siderophore-producing Pseudomonas sp. lowered the maximum cell level of S. putrefaciens 1-2 log units from 10(9) to 10(10) cfu g-1 when the strains were grown on fish muscle blocks at 0 degrees C but the growth rate of S. putrefaciens was not affected.

Agar↗
PubMed2001

A new multicolor-FISH approach for the characterization of marker chromosomes: centromere-specific multicolor-FISH (cenM-FISH).

Centromere-specific multi-color FISH (cenM-FISH) is a new multicolor FISH technique that allows the simultaneous characterization of all human centromeres by using labeled centromeric satellite DNA as probes. This approach allows the rapid identification of all human centromeres by their individual pseudo-coloring in one single step and is therefore a powerful tool in molecular cytogenetics. CenM-FISH fills a gap in multicolor karyotyping using WCP probes and distinguishes all centromeric regions apart from the evolutionary highly conserved regions on the chromosomes 13 and 21. The usefulness of the cenM-FISH technique for the characterization of small supernumerary marker chromosomes with no (or nearly no) euchromatin and restricted amounts of available sample material is demonstrated in prenatal, postnatal, and tumor cytogenetic cases. In addition, rarely described markers with the involvement of heterochromatic material inserted into homogeneously staining regions could be identified and characterized by using the cenM-FISH technique.

Centromere↗
PubMed1975

[Quantitative determination of trace metals in frozen fish, fish oil and fish meal (author's transl)].

As a contribution to the discussion on the "Verordnung uber Umweltkontaminanten" (draft) we examined 73 double samples of the frequent types of edible fish and 2 samples each of fish oil and fish meal for their content of arsenic (As), cadmium (Cd), lead (Pb) and mercury (Hg). Arsenic was determined photometrically, cadmium and lead by anodic stripping voltammetry and mercury by flameless atomic absorption spectrometry. We found the following average contents (ppm): 0.84 As, 0.003 Cd, 0.08 Pb, 0.06 Hg. The limits (ppm) set up in the draft for a German "Höchstmengenverordnung" (regulation specifying the maximum concentrations allowed) were by far not reached in the case of Cd, Pb, and Hg with all samples while they were fulfilled in the case of As by only 18% of the samples. It seems that the limit for As of 0.5 ppm has been set up too low. In the average, the following proportion applies for the trace contents found: Cd:Hg:Pb:As = 1:20:27:280. Correlations between the contents of trace metals on the one hand and fishing grounds or trawler or sample piece on the other could not be found.

Arsenic↗
PubMedDate not supplied

One fish, two fish, we QC fish: controlling data quality among more than 50 organizations over a four-year period.

EPA is conducting a National Study of Chemical Residues in Lake Fish Tissue. The study involves five analytical laboratories, multiple sampling teams from each of the 47 participating states, several tribes, all 10 EPA Regions and several EPA program offices, with input from other federal agencies. To fulfill study objectives, state and tribal sampling teams are voluntarily collecting predator and bottom-dwelling fish from approximately 500 randomly selected lakes over a 4-year period. The fish will be analyzed for more than 300 pollutants. The long-term nature of the study, combined with the large number of participants, created several QA challenges: (1) controlling variability among sampling activities performed by different sampling teams from more than 50 organizations over a 4-year period; (2) controlling variability in lab processes over a 4-year period; (3) generating results that will meet the primary study objectives for use by OW statisticians; (4) generating results that will meet the undefined needs of more than 50 participating organizations; and (5) devising a system for evaluating and defining data quality and for reporting data quality assessments concurrently with the data to ensure that assessment efforts are streamlined and that assessments are consistent among organizations. This paper describes the QA program employed for the study and presents an interim assessment of the program's effectiveness.

Animals↗
PubMed1999

From fish to FISH: the comparative pathology of neuroblastomas in humans, mice and fish.

Neuroblastoma is a malignant cancer of the sympathetic nervous system and is one of the most frequent solid cancers in young children. Only a few of the many advances in our understanding of basic genetic and cellular mechanisms leading to neuroblastoma development have translated to clinical practice, and the prognosis for children with neuroblastoma, particularly at advanced stages, has remained poor. Major directions for neuroblastoma management and control include the application of prognostic parameters, particularly amplified MYCN, which can be readily visualized by chromosomal fluorescence in situ hybridization (FISH), for individual therapy design, as well as the initiation of a presymptomatic screening program for early tumor detection to reduce the fraction of advanced-stage tumors. In addition, new and innovative therapeutic approaches are being sought. The understanding of molecular and cellular pathways resulting in spontaneous regression in up to 10% of neuroblastoma patients, possibly by apoptosis, could provide the basis for new biologically based therapeutic interventions. Unlike most other pediatric cancers, neuroblastoma can be studied in two experimental animal systems. One is the fish system Xiphophorus, where neuroblastomas can be induced in specific strains by exposure to mutagens/carcinogens; the second is mice that carry MYCN as a transgene. These animal systems demonstrate that neuroblastomas are evolutionarily conserved tumors. Their study could well result in a better understanding of neuroblastoma development. At the same time they represent systems in which experimental therapies can be preclinically tested.

Animals↗
PubMed1989

Polyunsaturated fatty acid metabolism in fish cells: differential metabolism of (n-3) and (n-6) series acids by cultured cells originating from a freshwater teleost fish and from a marine teleost fish.

1. The incorporation and metabolism of (n-3) and (n-6) polyunsaturated fatty acids (PUFA) supplemented to growing cultures were studied in rainbow trout (RTG-2) and turbot (TF) cell lines. 2. A fatty acid concentration of 20 microM considerably altered the fatty acid composition of the cells without affecting lipid class composition or the appearance of cytoplasmic lipid droplets. 3. Both cell lines exhibited considerable delta 6 desaturase activities. 4. Whereas delta 5 desaturase activity was expressed in RTG-2 cells, delta 4 desaturase activity was absent and, conversely, delta 4 desaturase activity was expressed in TF cells, but there was an apparent deficiency in the C18 to C20 elongase multi-enzyme complex. 5. The delta 6 desaturase activity in both cell lines showed little preference between 18:2(n-6) and 18:3(n-3) but the delta 5 desaturase activity of RTG-2 cells and the delta 4 desaturase activity of TF cells showed a preference for (n-3)PUFA. 6. Two fish oil concentrates were assessed for their ability to generate fatty acid compositions in the cell lines more closely resembling those of intact fish tissues.

Animals↗
PubMed2000

IgE antibody to fish gelatin (type I collagen) in patients with fish allergy.

BACKGROUND: Most children with anaphylaxis to measles, mumps, and rubella vaccines had shown sensitivity to bovine gelatin that was included in the vaccines. Recently, it was found that bovine type I collagen, which is the main content in the gelatin, is a major allergen in bovine gelatin allergy. Fish meat and skin also contain type I collagen. OBJECTIVE: The present study was designed to investigate IgE antibody to fish gelatin in children with fish allergy. METHODS: Serum samples were taken from patients in 3 groups: (1) 10 patients with fish allergy and specific IgE to fish meat; (2) two patients with allergies to both fish meat and bovine gelatin and specific IgE to fish meat and bovine gelatin; and (3) 15 patients with atopic dermatitis and specific IgE to fish meat. Various fish gelatins (type I collagen) were prepared from fish skin. IgE antibody to fish gelatin was analyzed by using ELISA and immunoblotting. RESULTS: Of 10 patients with fish allergy, 3 had specific IgE to fish gelatin. Of two patients with fish allergy and bovine gelatin allergy, all had specific IgE to fish gelatin. Of 15 patients with atopic dermatitis and specific IgE to fish meat, 5 had specific IgE to fish gelatin. Furthermore, IgE from pooled serum of the patients reacted with both the alpha1 and alpha2 chains of fish type I collagen in immunoblots. There is cross-reactivity among gelatins from various fishes, but there is little cross-reactivity between fish and bovine gelatins. CONCLUSION: Some fish-sensitive patients possessed IgE antibody to fish gelatin. Fish gelatin (type I collagen) might be an allergen in subjects with fish allergy.

Adolescent↗
PubMed1996

Immunopathogenesis of fish allergy: identification of fish-allergic adults by skin test and radioallergosorbent test.

BACKGROUND: As the consumption of fish increases in the United States, the importance of allergic reactions to fish has become clear. Since most previous studies on fish allergy have focused on children reacting mainly to codfish, there is a need to investigate allergic reactions to other fish in adults. OBJECTIVE: To identify fish-allergic adults, and to assess cross-reactivity among different species of fish by RAST inhibition. METHODS: Thirty-nine individuals who reported fish allergy were selected for study; 32 (82%) were atopic as defined by two or more positive skin tests to common inhalant allergens and a history of allergic reactions and 33 (85%) experienced allergic symptoms within 30 minutes after ingesting fish. The most frequently reported symptoms were hives (69%), itching (69%), and wheezing/chest tightness (54%). Study subjects were skin tested with fish extracts and their sera assayed for IgE antibodies to different fish species. RESULTS: Thirty-six (92%) of the subjects tested had a positive skin test to at least one of 17 fish extracts tested; 9/35 (26%) reacted to all 17 extracts. Of the atopic (two or more positive skin tests to common inhalant allergens plus a personal and/or family history of allergy) and nonatopic fish-tolerant controls, 20/26 (77%) reacted by skin test to one or more fish extracts tested; the most prevalent positive reaction was to anchovy (73%). A significant correlation (P < .01) was observed between skin test reactivity of fish-allergic subjects to most fish extracts and fish RAST reactions. Radioallergosorbent inhibition testing demonstrated significant cross-reactivity among pollack, salmon, trout, and tuna; and between mackerel and anchovy. CONCLUSION: These results suggest that fish-allergic subjects may be clinically sensitive to more than one species of fish. Skin test reactivity to fish by itself is not an adequate criterion for the confirmation of clinically relevant fish allergy; consequently, fish-allergic subjects with positive skin tests to several fish species should exercise caution when eating fish until tolerance can be demonstrated by double-blind, placebo-controlled food challenge, at the patient's earliest convenience.

Adult↗