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Mitsuokella apri sp. nov., a novel species of the family Selenomonadaceae isolated from faeces of a wild boar (Sus scrofa).

A Gram-stain-negative, rod-shaped, obligately anaerobic strain, designated WILCCON 0060T, was isolated from the faecal sample of a wild boar (Sus scrofa) collected on the Island of Ubin (Pulau Ubin), Singapore (1.4126°N, 103.9577°E). Phylogenetic and comparative analyses based on 16S rRNA gene and whole-genome sequences indicated that strain WILCCON 0060T belongs to the genus Mitsuokella, within the family Selenomonadaceae. Notably, the average nucleotide identity and digital DNA-DNA hybridization values between strain WILCCON 0060T and the type strains of two existing Mitsuokella species, Mitsuokella multacida DSM 20544T and Mitsuokella jalaludinii DSM 13811T, only ranged from 84.6-84.9% and 24.1-24.7%, respectively, both well below the recommended species delineation thresholds of 95.0% and 70.0%. Supported by additional physiological, biochemical and genomic differences, these findings collectively indicate that strain WILCCON 0060T represents a novel species within the genus Mitsuokella. We propose the species name Mitsuokella apri sp. nov., pertaining the isolation of the type strain WILCCON 0060T (= DSM 120052T = LMG 33761T) from a faecal sample of a wild boar.

Animals↗

[Capillaria (Nematoda, Trichuridae) of wild swine (Sus scrofa) in Austria].

Capillaria (Nematoda, Trichuridae) of wild boar in Austria. In a research on Capillaria species with wild boar in Austria 2 species could be found, C. spec. in the stomach and C. garfiai in the tongue. The morphological characteristics of both species were described and represented C. spec. could only be detected in one of 105 stomachs and intestines. C. garfiai, however, could be proved in 77 (= 69%) of 112 examined tongues. C. garfiai which parasitizes in the tongue epithelium causes pathological changes only to a slight degree, so that it is of almost no pathogenic significance. The frequency of infestation with eggs of Capillaria (with a high probability of C. garfiai) was 14.4% of 319 examined samples of faeces. The validity of C. spec. is discussed. Both species were detected at wild boar in Austria for the first time.

Animals↗

Classical swine fever virus Strain 'C'. How long is it detectable after oral vaccination?

To determine the persistence period of C-strain vaccine virus in immunized animals, domestic pigs and wild boars were vaccinated orally and killed on different days post vaccinationem (dpv). Tissue samples were taken at necropsy from both species for detection of C-strain virus. From domestic pigs nasal swabs and faeces were also collected. During the investigation period (2-12 dpv) vaccine virus could never be detected in nasal secretions and in faeces of vaccinated domestic pigs. In contrast, C-strain virus was found in organs until day 8 pv in domestic pigs and until day 9 pv in wild boars. Whereas in domestic pigs virus was detected in tonsils, Ln. mandibularis or in spleen, in wild boar it only was found in tonsils. We conclude that C-strain vaccine virus is not detectable in wild boars longer than 10-12 days after intake of the vaccine baits.

Administration, Oral↗

Oral immunisation of swine with a classical swine fever vaccine (Chinese strain) and transmission studies in rabbits and sheep.

Seven experiments including a total of 47 pigs, 11 wild boars, 26 rabbits, 10 hares and 16 sheep were carried out to assess the efficacy, safety and transmission of the Chinese vaccine strain of the classical swine fever virus (CSFV) administrated by the oral route. Within 3 weeks after oral vaccination, a clear seroconversion occurred in the pigs. Six weeks after vaccination, vaccinated pigs were fully protected against a virulent challenge. The C-strain was not isolated from tonsils, spleen, lymph nodes, thymus, saliva, urine and faeces of pigs within 4 days after oral vaccination. In one experiment, susceptible pigs were placed in direct contact with vaccinated pigs. None of these contact-exposed pigs became serologically positive for CSFV antibodies. It is concluded that the C-strain induces protection in pigs when administrated by the oral route and is not shed by vaccinated pigs. Serum anti-CSFV antibodies developed in seven out of eight wild boars vaccinated by the oral route. No vaccine virus was detected in the spleen and tonsils of these animals. The results in wild boar were in accordance with those obtained in domestic pigs. Sheep did not show any clinical signs after oral vaccination while rabbits had moderate hyperthermia and growth retardation. No clinical response to oral immunisation in hares was detected. At the end of the experiment, no sheep had detectable serum antibodies against CSFV, whereas a few vaccinated rabbits and hares became seropositive. None of the contact-exposed rabbits and hares seroconverted. These data indicate that the C-strain is safe for sheep and as expected, moderately or not pathogenic for rabbits and hares. These efficacy and safety studies on oral vaccination with the C-strain under experimental conditions provide essential information for further studies in wild boars under experimental and field conditions, including assays with baits to control a CSF epidemic.

Administration, Oral↗

[Final host specificity of sarcocystis species transmitted by dogs (authors's transl)].

Dogs and raccoons were fed muscle of various intermediate hosts that had been infected either naturally or artificially wit Sarcocystis species transmitted from dogs. The muscle samples used for the experiments contained cysts of the following species: S. sp. of the donkey, S. bovicanis of cattle, S. ovicanis and S. sp. of sheep, S. suicanis of pigs, or S. capracanis and S. sp. of goats. In all trails, the dogs shed sporocysts of the Sarcocystis species concerned after a typical prepatency. In the faeces of the raccoons sporocysts were only found after they were fed on porcine muscle containing S. suicanis. The prepatency in raccoons was 9 days. Both the prepatent period of S. suicanis and the size of the excreted sporocysts were identical in dogs and raccoons. Sporocysts isolated from raccoon faeces were infective for pigs. Thus the raccoon was established as an additional final host of S. suicanis, and may be of significance in the epidemiology of this parasite in the European wild boar.

Animals↗

Role of birds in transmission of classical swine fever virus.

Active transmission of classical swine fever virus (CSFV) was studied in six birds (five ravens, one hooded crow) and two laying hens. Cloacal swabs, blood and organs of birds and hens as well as blood and organ samples of pigs which had been fed with faeces derived from CSFV infected birds or which had come in contact with faeces of infected hens were negative for CSFV. None of the animals seroconverted during the study. This result demonstrates that active virus transmission by these animals is unlikely. Dissemination of CSFV from wild boar to domestic pigs is discussed.

Animals↗

Epidemiology of porcine reproductive and respiratory syndrome (PRRS): an overview.

PRRS disease was first recognised in the USA in 1987 and in Europe in 1990 and since then the disease has spread widely throughout many pig-producing countries. After a severe epidemic phase, the infection has become endemic. The prevalence of infection is generally high in infected countries. However, in areas with a low density of pigs, infection may spread slowly and if infected animal movements are not significant, farm-to-farm spread can be controlled and prevalence of infection maintained at a low level. The PRRS virus (PRRSV) was completely unknown before 1986, and the question of its origin remains unanswered. The exact epidemiologic relationship between American and European strains of PRRSV is difficult to establish because different isolates appear to belong to two distinct sub-populations which are only distantly antigenically related. In the environment, virus survival is optimal when temperature is cold and when ultra-violet light exposure is low (little sunshine). These conditions are easily attained in winter and that may explain why virus spread increases during this period. Pigs of any age (including wild boars) are the only animals known to be naturally infected with PRRSV. Relatively close contact between pigs is the primary factor in virus transmission. Aerial transmission is a second mechanism of spread, particularly in winter and particularly over distances of less than 3 km. A third route of transmission is via semen. The role of fomites is not clearly documented, however since the virus is excreted in faeces and urine, slurry should be considered as a potential source of contamination. Within herds, the virus spreads rapidly with up to 85 to 95% of pigs in a herd becoming sero-positive within two to three months. Thereafter, virus activity persists for extended periods (several month to years). Nevertheless, some authors have reported spontaneous elimination of PRRSV from infected farms. For the future, there remain questions concerning the possible evolution of the disease (in terms of its sanitary and economic impacts), and the possible influence of vaccines on the epidemiological features of PRRS.

Animals↗

Porcine coccidia in Papua New Guinea.

Faecal samples from 232 domestic pigs raised on concrete, 98 free-ranging village pigs, and five wild boar showed 46.6 (108/232), 54 (53/98) and 80% (4/5) prevalence of coccidian oocysts, respectively. Eight species of Eimeria, and Isospora suis, were recovered. In their descending order of predominance in the pigs raised on concrete, the species of coccidia were E. debliecki (26.7%), E. scabra (22.4%), E. neodebliecki (19.8%), E. porci (15.5%), E. suis (11.6%), E. polita (8.6%), E. perminuta (7%), E. spinosa (5.6%) and I. suis (3.9%). The first five species listed above predominated in the village pigs as well. E. polita, E. spinosa and I. suis were not found in the wild boar. I. suis oocysts prevailed in 8.3% of the 36 sows on concrete, and in 11.1% (3/27) of those which were positive for coccidia. Isosporoid oocysts were absent in the village sows. Of the 125 less than 24-day-old piglets, 29.6% were diarrhoeic, and of these, 43.2% were positive for coccidia. Four of the 16 (25%) coccidia-positive, diarrhoeic piglets, and four of the 37 (10.8%) coccidia-positive non-diarrhoeic piglets shed I. suis oocysts, an observation which seems to weaken the present contention that I. suis is the primary causative agent of neonatal porcine coccidiosis. The highest mean number of oocysts per gram faeces (23,550) was recorded from the diarrhoeic farm piglets on concrete, and the lowest of 6,100 from the gestating farm sows. Mean opg data revealed very little significant quantitative variation between the corresponding age groups of the free-ranging village pigs and the commercially-farmed ones. One of the most interesting findings in the study was that the sows were more frequently infected than all other age groups.

Animals↗