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[Arsenic contamination in imported sea fish and fish products made from them].

The rate of arsenic contamination in sea-fish is, in comparison with freshwater fish, relatively high. An investigation of five kinds of sea-fishes--of mackerel, herring, cod, tunny, and plaice--that are most frequently put on the market showed that a permitted value up to 1 mg As per 1 kg of fish meat was found only in 24.0% of mackerel, 9.5% of herring, 33.4% of cod, 57.0% of plaice and 0.0% of tunny. Further investigation of dressed fish--of fillets--revealed that 15.8% of herring fillets and 0.0% of cod fillets met the permitted value. The values of a majority of studied sea-fish samples ranged from 1 to 2 mg As per 1 kg: 52% of mackerel, 63.5% of herring, 66.6% of cod, 43% of tunny, 28% of plaice. It is necessary to revalue the permitted values of arsenic content in sea-fish and in products made from their meat and to defend the upper limits of the permitted arsenic value, which will require the purposeful organization of effective laboratory tests the results of which will directly influence an estimation of fish raw material and will provide the corresponding hygienic quality of fish and fish products.

Animals

[Total and organically bound mercury content in fishes from German fishing grounds (author's transl)].

In a survey sponsored by the Ministry of Food Agriculture and Forestry of the Federal Republic of Germany the mercury contamination of 759 specimens of fishes from the German fishing grounds were analysed. The determinations were performed by flameless atomic absorption (cold vapour system). -We found for the fishes of greatest economic importance herring, cod, saithe, haddock very low averages of less than 0,1 ppm. The legal limit of 1 ppm was only reached or exceeded by older fishes (ling 1.18 ppm, red-fish 1.09 ppm) and by specific kinds of fishes (shark 3.23 ppm, ray 1.69 ppm, tuna 0.80 ppm). -The organically bound mercury (methyl-Hg) which is considered especially toxic, has been determined by gas-liquid-chromatography. Methyl-Hg constitutes about 70--98% of the total mercury, a value which is in agreement with Japanese and Swedish reports on this subject.

Animals

[Nutritional value of fish flours made in laboratory from fishes of the Nosy-Bé area].

Several types of fish meals (principally from no commercializable Carangues) are made in a laboratory. They are tested for general composition, amino-acid composition, vitamin and mineral compositions. Two fish meals from Carangues are very interesting: they are white muscle meal (rich in proteins of good quality, poor in lipids and slightly rich in minerals) and the meal from fish without head and guts (not so rich in proteins but slightly richer in minerals). These fish meals have a variable interest regarding mineral and vitamin contents. All of them have a lack of methionin and cystin which is comparable to the defficiency mentioned in other fish meals by various authors.

Amino Acids

[Extractability of lipids from fish and fish products].

The completness of the lipids removal from the fish muscles and fish products was investigated by making extraction in a filtering separating funnel (FSF) formerly proposed for determining lipids in oil-bearing seeds and cereals. The new modified procedure was contrasted against the standard one (accepted by the State Standards) used in separating and quantitative determination of the lipids content in fish by using the Socklet apparatus for extraction. The proposed variant of extraction was found to secure a higher yield of the lipids. The pattern of analysis used in determining the lipids content in vegetable products may well be used also in investigating animal products, such as, fish and fish products.

Animals

A new highly effective ovulating agent for fish reproduction. Practical application of LH-RH analogue for the induction of spawning of farm fishes.

The synthetic analogue of the nonapeptide LH-RH has been found to be highly effective in the induction of spawning of farm fishes (the grass carp, the silver carp, the spotted silver carp, and the black carp.) Its biological activity is many times higher than that of the synthetic decapeptide or natural releasing hormone. Out of a total number of 500 mature fishes treated with the nonapeptide alone and/or combined with a minimum amount of fish pituitary, 396 of them spawned--with an over-all spawning rate of 78%. As far as we know, this is probably the most effective ovulating agent or hormone now available for fishes. This finding is of paramount importance in pisciculture for large-scale production of fry. The recommended dosages for the following farm fishes are: the grass carp--1-10 microgram/kg b.w.; the silver carp--3 microgram or more/kg b.w. in divided doses; the spotted silver carp--1.4 microgram or more/kg b.w.; and the black carp--10 microgram/kg b.w. For the latter species, the efficacy of the peptide could be improved by a concurrent administration of 0.5-2 mg of the pituitary gland. The significance of this work in piscicultural practice and the mechanism of hormonal action are briefly discussed.

Animals

A disease of freshwater fishes caused by Tetrahymena corlissi Thompson, 1955, and a key for identification of holotrich ciliates of freshwater fishes.

Tetrahymena corlissi, a free-living protozoan, apparently caused the death of large number of guppies (Poecilia reticulatus) and occasionally other fishes, in aquaria and hatcheries at several locations. Apparently the disease occurs when the fish and protozoan populations are both at a high level of density. The signs include white spots and epidermal damage. Histologically, T. corlissi could be seen in skin, muscle, and viscera; in some there was marked inflammation, in others there was little tissue reaction. A key is presented for the identification of invasive fish ciliates: Chilodonella, Hemiophrys, Ichthyophthirius, Ophryoglena, and Tetrahymena.

Animals

Survey on Anisakid larvae in marine fish from fish markets in Jakarta.

Anisakid parasites were found in fish commonly sold in the markets of Jakarta. The larvae found were Anisakis type I and Terranova type B. The rate of infection of these two different Anisakid larvae infecting different species of fish was discussed. To date, no human infection has as yet reported in Indonesia. This may be due to the fact that Indonesians do not usually eat raw fish.

Animals

Studies on ecophysiology of fish parasites: effect of trypanosome infection on the serum cholesterol levels of fishes.

Serum cholesterol levels of six species of fresh water fishes were lowered due to trypanosome infection. The percentage of infection in the different species of hosts ranged from 3.27% in Clarias batrachus to 16.66% in Wallago attu. Loss in serum cholesterol level was highest (42.39%) in the spiny eel Mastocembelus armotus and lowest in the carp Cirrhina mrigala (4.56%). Thus hypocholesterolemia was evidently caused in the fishes by trypanosomes.

Animals

[Determination of the vitamin E in fish and fish products].

To measure the tocopherols content in fish and fishery products conditions of analysis permitting the utilization of the Emmiry-Engel method were selected. Subsequent to a comparative investigation, a system of solvents was sorted out with which in column chromatography using aluminium oxide the most complete separation of tocopherols from compounds hindering colourimetric determination could be achieved. The procedures used in determinations and data on the tocopherols content in 27 species of fish, fishery products and others are given.

Animals

Some properties of an established fish cell line from the marine fish, Caranx mate (Omaka).

A monolayer culture of fibroblast-like cells was initiated and established from a trypsin digest of Omaka fish larvae (Caranx mate). The cell culture has been subcultured 105 times over a period of 30 mo and appears to be contact-inhibited. The Omaka cells grew optimally at 27 degrees in Eagle's basal medium plus 10% fetal calf serum but failed to grow at 16 and 37 degrees. At the lower temperature the cells remained viable and began to divide when the temperature was raised to 27 degrees. Unlike certain marine fish cell lines the Omaka cell did not require a higher osmolarity medium for optimal growth. Karyological analysis at the 43rd passage revealed a chromosomal modal number of 50 consisting of 7 submetacentric pairs and 18 acrocentric pairs of chromosomes. Virological studies indicated that Omaka cells supported the replication of poikilothermic viruses such as, FV-3 and IPN viruses, but not the mammalian viruses examined. Attempts to induce interferon by the synthetic polynucleotide poly I:C were unsuccessful.

Animals

Gene action in fish of tetraploid origin. IV. Ribosomal DNA amount in clupeoid and salmonoid fish.

Phylogenetically tetraploid species of the fish order Isospondyli generally have twice the mean ribosomal gene content as closely related species on the phylogenetically diploid level. Considerable intraspecific variation of rDNA amount was observed. These findings are discussed in view of the hypothesis that selective loss of ribosomal genes may account for diminishing genic activity in phylogenetically tetraploid organisms.

Animals

Development of an enzymatic method to differentiate fresh and sea-frozen and thawed fish fillets. I. Comparison of the applicability of some enzymes of fish muscle.

The activities of mitochrondrial and lysosomal enzymes in press juices of fresh and frozen and thawed fillets were compared. It was demonstrated that several lysosomal enzymes are suitable for the detection of thawed fillets, because their activities in press juice increased considerably in consequence of freezing and thawing fish fillets.

Acid Phosphatase

Carotenoids in fish. XVIII--carotenoids in the brain of some fishes.

Using column and thin-layer chromatography the occurrence of various carotenoids was studied in the brain of some (11) fishes. In result of the analyses the presence of the following carotenoids has been established: beta-zeacarotene, canthaxanthin, cryptoxanthin, alpha-cryptoxanthin, isocryptoxanthin, lutein, lutein-5,6-epoxide, zeaxanthin, isozeaxanthin, tunaxanthin, flavoxanthin, astaxanthin ester, 4-hydroxy-alpha-carotene, 4-keto-alpha-carotene and unknown xanthophylls.

Animals