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Results for “GLUCOSE, RADIOACTIVE”

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At least 19 recordsLinked to original sources
PubMed1981

Rapid and specific isolation of radioactive glucose from biological samples.

An easy, reliable, and specific ion-exchange method is presented for isolating glucose for specific radioactivity determinations from both blood plasma and buffered in vitro incubation media. The use of a glucose binding resin (borate-charged anion resin) combined speed of ion exchange with specificity of derivative formation. Glucose specific radioactivities, determined by ion exchange on protein-free filtrates of plasma containing [carbon-14] glucose, show excellent agreement with those from the popular glucose pentaacetate derivative method and are less variable. Carry-over of labeled acetate, propionate, lactate, glyoxylate, alanine, aspartate, or glutamate into the glucose fraction is less than .2%. Glycerol carryover is 1.2%. Glucose recovery is increased about three times that of the glucose pentaacetate derivative method and averaged 94% from plasma filtrates.

Animals↗
PubMed1975

Biosynthesis of gastric mucins. In vitro incorporation of radioactive glucose.

An in vitro model for the study of the mechanism of gastric-glycoprotein (mucin) biosynthesis is described. Gastric mucosa scrapings were incubated in Eagle's minimum medium with U-14C-glucose. Linear incorporation-kinetics were observed during 4 hours. U-14-C-glucose was incorporated into soluble and cell bound glycoproteins. In the acid hydrolysate of soluble and cell bound fractions all the amino acids separated by high voltage electrophoresis and all the carbohydrates separated by thin layer chromatography contained appreciable radioactivity showing the active conversion of the added radioactive glucose to all the amino acid and carbohydrate precursors used by the gastric cells for the biosynthesis of macromolecular glycoprotens. The carbohydrate/protein ratio of the excreted glycoproteins increases steadily during the 4h incubation period suggesting that the chemical composition of the excreted mucins changes during this period. The cell bound glycoprotein fraction retains a constant composition during the 4th incubation period. The high fucose to hexose ratio of the soluble (excreted) glycoproteins suggests that they are probably identical to the gastric mucins isolated from native gastric preparation. This model is therefore considered a valid one for the study of the regulation of the biosynthesis of gastric mucins as well as for the study of drug action.

Amino Acids↗
PubMed1980

The synthesis of neuroactive amino acids from radioactive glucose and glutamine in the rat retina: effects of light stimulation.

The effect of light stimulation in vitro on the labelling of neuroactive amino acids derived from [14C]glucose or [14C]glutamine in the rat retina has been studied. [14C]Glutamine, at 700 microM, provided about 50% of the tissue pools of glutamate, aspartate, and GABA; and the labelling of these decreased on light stimulation, both in the photoreceptor cells (glu and asp) and in the inner retina (glu, asp, and GABA). In contrast, there were no significant changes in the entry of label derived from [14C]glucose, although similar trends were apparent in the data obtained for the photoreceptor cell layer. The pools may, therefore, be separate. Other results support the contention that glucose is the principal energy source for the retina, its entry into non-amino acid derivates being decreased on light stimulation.

Animals↗