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At least 19 recordsLinked to original sources
PubMed1989

Cells from Rana pipiens gastrulae and arrested hybrid gastrulae show differences in adhesion to fibronectin-sepharose beads.

Experiments were performed to examine adhesion of Rana pipiens gastrula cells and arrested hybrid gastrula cells to fibronectin-Sepharose beads (FN-beads). Blastula cells from both normal and hybrid embryos show poor adhesion to FN-beads. Beginning at the early gastrula stage, however, normal cells show a progressively increasing tendency to adhere to beads. In two different arrested hybrid embryos, cells from all developmental stages lack the ability to adhere to beads. A third hybrid shows an increase and then a decrease in cell-bead adhesion. A fourth hybrid shows a late increase in cell-bead adhesion in animal-half cells and no increase at all in vegetal-half cells. Blastula-stage cells have the ability to adhere to con A-beads and two kinds of Cytodex beads but will not adhere to FN-beads. Similarly, some cells from arrested hybrid embryos lack the ability to adhere to FN-beads but will adhere to con A-beads and cytodex beads. Observations in the light and scanning electron microscope show that normal cells form lamellipodia on FN-beads and move about actively on them, much like they do in vivo on surfaces coated by fibrils containing fibronectin. For adherent hybrid cells attached to beads, one kind does so by small pseudopodia but does not move on them and another kind forms active lamellipodia at the tips of fusiform cells and moves on beads.

Animals↗
PubMed1990

Cell adhesion to extracellular matrix in normal Rana pipiens gastrulae and in arrested hybrid gastrulae Rana pipiens female X Rana esculenta male.

Rana pipiens eggs fertilized by Rana esculenta sperm (ESC) hybrid embryos develop until gastrulation in control Rana pipiens embryos (PIP) and then show morphogenetic arrest. After arrest, ESC do not gastrulate but live for 5 days as blastula-like embryos. We studied the distribution of fibronectin (FN)-containing fibrils and integrin (INT) in PIP and ESC. There are many FN-fibrils in PIP organized in anastomosing networks radiating away from the center of individual cells and across intercellular boundaries. ESC have fewer fibrils compared to PIP. These fibrils are first located between cells in disorganized arrays. After arrest in ESC, when PIP are Stage 14 neurulae, many more FN-fibrils appear. INT-staining occurs in both embryos in similar patterns. In xenoplastic transplantations, we found that the extracellular matrix on the inner surface of the ESC blastocoel roof serves as a substratum for PIP cell migration. In an in vitro assay, we found more cell adhesion to FN-substrata in PIP than in ESC. Cell locomotion rates on FN-substrata were 1.70 +/- 0.85 microns/min for PIP but only 0.46 +/- 0.56 microns/min for ESC. We also found that the inner surface of the blastocoel roof from ESC can not promote cell adhesion and locomotion when Stage 11 fragments are used for conditioning but that Stage 14 fragments can deposit a FN-fibril-rich extracellular matrix which supports PIP mesodermal cell migration at a rate of 1.26 +/- 0.38 microns/min.

Animals↗
PubMed2001

The organizer of the mouse gastrula is composed of a dynamic population of progenitor cells for the axial mesoderm.

An organizer population has been identified in the anterior end of the primitive streak of the mid-streak stage embryo, by the expression of Hnf3beta, Gsc(lacZ) and Chrd, and the ability of these cells to induce a second neural axis in the host embryo. This cell population can therefore be regarded as the mid-gastrula organizer and, together with the early-gastrula organizer and the node, constitute the organizer of the mouse embryo at successive stages of development. The profile of genetic activity and the tissue contribution by cells in the organizer change during gastrulation, suggesting that the organizer may be populated by a succession of cell populations with different fates. Fine mapping of the epiblast in the posterior region of the early-streak stage embryo reveals that although the early-gastrula organizer contains cells that give rise to the axial mesoderm, the bulk of the progenitors of the head process and the notochord are localized outside the early gastrula organizer. In the mid-gastrula organizer, early gastrula organizer derived cells that are fated for the prechordal mesoderm are joined by the progenitors of the head process that are recruited from the epiblast previously anterior to the early gastrula organizer. Cells that are fated for the head process move anteriorly from the mid-gastrula organizer in a tight column along the midline of the embryo. Other mid-gastrula organizer cells join the expanding mesodermal layer and colonize the cranial and heart mesoderm. Progenitors of the trunk notochord that are localized in the anterior primitive streak of the mid-streak stage embryo are later incorporated into the node. The gastrula organizer is therefore composed of a constantly changing population of cells that are allocated to different parts of the axial mesoderm.

Animals↗
PubMed1993

Basic fibroblast growth factor induces differentiation of neural tube and neural crest lineages of cultured ectoderm cells from Xenopus gastrula.

The vertebrate nervous system is initially induced from a section of dorsal ectoderm by signal(s) from the underlying dorsal mesoderm during gastrulation. In an effort to identify the neural inducing factor(s) emanating from the dorsal mesoderm, we have examined the inductive action of various growth factors by applying them to ectoderm cells from Xenopus gastrulae (8- to 12.5-hour age; embryonic stage 9+ to 11 1/2) in a microculture system. Monoclonal antibodies that specifically recognize cellular differentiation antigens from three distinct ectoderm lineages (N1 for CNS neurons from neural tube, Me1 for melanophores from neural crest and E3 for skin epidermal cells from epidermal lineages, respectively) and a mesoderm lineage (Mu1 for muscle cells) were used as markers to monitor the differentiation of cultured ectoderm cells. We found that basic fibroblast growth factor (bFGF) was capable of specifically and reproducibly inducing gastrula ectoderm cells to produce CNS neurons and melanophores at concentrations as low as 5 pM, a value about 50-fold lower than that required to induce the formation of muscle cells from blastula animal cap cells (6-hour age; stage 8+). The induction of neural lineages by bFGF was correlated with a suppression of epidermal differentiation in a dose-dependent manner. bFGF never induced the formation of muscle cells from gastrula ectoderm cells even at concentrations as high as 5 nM. The response of ectoderm cells to bFGF changed dramatically during gastrulation. Ectoderm cells from early (8- to 9-hour age; stage 9+ to 10) gastrula gave rise to CNS neurons, but yielded few melanophores. As ectoderm cells were prepared from gastrulae of increasing age, their competence to form neurons was gradually lost, whereas the production of melanophores was enhanced and peaked in 11-hour gastrula (stage 10 1/2). The ability to form both neurons and melanophores was substantially reduced in 12.5-hour gastrula (stage 11 1/2). By examining ectoderm cells from the ventral and dorsal sides independently, it was also shown that during gastrulation the change in response to bFGF of the ventral ectoderm preceded that of the dorsal ectoderm. The state of competence of the ectoderm changed primarily due to intrinsic factors rather than by instruction from other parts of the gastrula embryo. This was shown by adding bFGF to cultures of ectoderm cells that were isolated at 9-hour (stage 10) and cultured for increasing periods to allow their autonomous development. The time course of both loss of neuronal competence and gain and loss of melanophore competence closely paralleled that observed in vivo during gastrulation.(ABSTRACT TRUNCATED AT 400 WORDS)

Animals↗
PubMed1976

Nucleotides in embryos in the stages of morula, gastrula, and neurula.

Levels of nucleotides and sugar nucleotides in embryos of Bufo arenarum at the stages of morula, gastrula, and neurula have been measured. The total amounts of purine nucleoside diphosphates decreased from morula to gastrula, but increased sharply from gastrula to neurula. The levels of ADP followed this pattern, but those of GDP did not change significantly through the three stages. Purine nucleoside triphosphate levels, which had increased immediately after fertilization, remained almost constant through morula, gastrula, and neurula. As with the purine nucleoside diphosphates, the adenine nucleotide decreased from morula to gastrula, and increased from gastrula to neurula. In contrast, the level of GTP showed a sharp maximum at gastrula. The total pyrimidine nucleoside triphosphate did not change significantly from morula through neurula. As in previous stages of development, only uridine sugar nucleotides were detected. A sharp increase of the galactosyl ester of nucleotides was found at gastrula.

Adenosine Diphosphate↗
PubMedDate not supplied

[Concanavalin-binding proteins and cytokeratins in different tissues of the early amphibian gastrula (Rana temporaria, Xenopus laevis)].

Concanavalin A (con A), a lectin which specifically interacts with aD-mannose and aD-glucose, has a neutralizing effect on the explants of the early gastrula ectoderm of several amphibian species. Consequently, it was interesting to study con A-binding protein spectrum of the ectoderm and compare it to those of other early gastrula tissues. Animal pole ectoderm (APE), dorsal blastopore lip (DBL) and vegetal pole endoderm (VPE) were dissected from early gastrulae of Rana temporaria and Xenopus laevis. The extracts were subjected to SDS-PAGE with subsequent immunoelectroblotting on nitrocellulose membranes. The blots were sequentially treated with con A solution, horseradish peroxidase and diaminobenzidine. Spectra of the con A-binding glycoproteins were similar in APE, DBL and VPE of R. temporaria. Ten-twelve fractions with the molecular weight in the range from 30 to 150 kDa were stained in each blot. Fractions with the molecular weight of 150, 125, 104, 94 and 42 kDa showed more prominent lectin binding. Con A-binding protein spectra remained unchanged after freezing-thawing of the studied extracts, as well as after blots were treated with neuraminidase or sulphuric acid in order to remove sialic acid residues; the only exception was 42 kDa fraction. At the same time, a-methyl-D-mannoside pyranoside completely blocked con A binding by fractions of the studied extracts. In histological sections of R. temporaria early gastrula, all cells bound FITC-labelled con A. Similar data were obtained with tissues of X. laevis early gastrula. While electrophoretic pattern of X. laevis tissues drastically differed from that of R. temporaria, there were no significant differences between con A-binding protein spectra of X. laevis APE, DBL or VPE. Thus, all studied tissues of the amphibian early gastrula contain similar set of con A-binding proteins; however, only APE is capable of neutralization in response to con A action. These data favor our earlier assumption (see Mikhaĭlov et al., 1989) that con A reception and transmission of the corresponding signal do not determine the characteristics of the target cells response. APE, DBL and VPE extracts were assayed also for the presence of a protein similar to cytokeratin No. 8 characteristic of simple epithelia of mammals. Experiments were performed using immunoelectroblotting with monoclonal antibodies (mAB) against cytokeratin No. 8 from rat colon (mAB E2 and E7 kindly supplied by Dr. G. A. Bannikov). In R. temporaria embryos, cytokeratin 8 was detected in APE, but not in DBL or VPE. In X. laevis gastrulae all the tissues studied contained this cytokeratin.

Amphibians↗
PubMed1983

Comparative study of extracellular fibrils on the ectodermal layer in gastrulae of five amphibian species.

Previous studies have shown the presence of a network of extracellular fibrils on the inner surface of the ectodermal layer of the Ambystoma maculatum gastrulae. The alignment of the network along the blastopore-animal pole axis has suggested that the network of fibrils guides the migrating mesodermal cells in gastrulae by contact guidance. We have also shown that these fibrils can be deposited on substrata by explanted embryonic fragments and that substrata conditioned in this manner support directed cell migration. In this study, we found that the appearance of the fibrils in the embryos coincides with the start of cell migration towards the animal pole. Gastrulae of three urodele species examined (A. maculatum, A. mexicanum and Cynops pyrrhogaster) have similar dense networks of fibrils. Xenopus laevis gastrulae also have similar fibrils but fewer fibrils compared to urodele embryos. Rana pipiens gastrulae have very few extracellular fibrils. The scarcity of the fibrils in anuran species may be related to the differences in arrangement of mesodermal cells during migration.

Ambystoma↗
PubMed1982

In vitro studies of amphibian gastrula cells.

Experiments are presented to determine whether Rana pipiens gastrula cells differentiate in vitro as they do in vivo with respect to rate, morphology and spreading characteristics. Comparisons are made between presumptive germ layer cells isolated at late blastula, cultured for 24 h, and the same cell types form late gastrula cultured for 1 h (LeBlanc and Brick, 1981a). These cells are chronologically similar and should be morphologically similar at the end of their respective culture periods if in vivo and in vitro differentiation are proceeding in the same direction and rate. Five hour cultures of the same cell types were compared to 1 h cultures in media with enhanced Ca2+ (LeBlanc and Brick, 1981b) to study the role of Ca2+ as a modulator of spreading and production of cell protrusions, and the rate at which these occur, as these relate to morphogenetic movements. The data and inferences can be summarized as follows: (1) Some Rana pipiens late blastula and gastrula cells appear to differentiate morphologically in vitro similarly to their differentiation in vivo. (2) In vitro differentiation is autonomous indicating that determination occurred prior to late blastula, mid-gastrula or late gastrula depending on when cells were isolated. (3) The presence of two sub-populations is some germ layer cell isolate indicates determinative interactions had occurred prior to isolation. (4) Morphologic similarity of cells in standard culture for 5 h to the same cells in culture with additional Ca2+ for 1 h suggests that Ca2+ modulates spreading and projection formation and the rate at which these occur. (5) The upper cell surface of these cells may provide adhesive sites for plasmalemma projections from adjacent cells.

Animals↗
PubMed2005

Muscle specification in the Xenopus laevis gastrula-stage embryo.

Recent fate maps of the Xenopus laevis gastrula show that mesodermal tissue surrounding the blastopore gives rise to muscle (Keller [1991] Methods Cell Biol 36:61-113; Lane and Smith [1999] Development 126:423-434). In a significant deviation from earlier data, the new maps demonstrate that cells in the ventral half of the gastrula are precursors to a significant portion of trunk somites. However, these posterior somites are not formed until tadpole stages (stages 38-44). We therefore set out to determine the timing of muscle specification within the ventral half of the gastrula. Our approach was to generate a series of tissue explants from gastrula-stage embryos and then culture them to either stage 28 (tailbud) or stage 44 (tadpole). At each endpoint, the presence of muscle in explants was assessed with a muscle-specific antibody. Interestingly, we found that muscle tissue is detected in ventral explants. However, these explants must be cultured to the tadpole stage. This is perhaps not unexpected, as this is the point at which this tissue normally gives rise to muscle. We further show that muscle specification of the involuting marginal zone does not change over the course of gastrulation. Together, these results suggest that dorsalizing signals emanating from the midline during gastrulation are not necessary for muscle specification of the ventral half of the involuting marginal zone.

Animals↗
PubMed1994

Contribution of early cells to the fate map of the zebrafish gastrula.

Previously, a tissue-specific fate map was compiled for the gastrula stage of the zebrafish embryo, indicating that development subsequent to this stage follows a reproducible pattern. Here it is shown that each early zebrafish blastomere normally contributes to a subset of the gastrula and thus gives rise to a limited array of tissues. However, the final contribution that any early blastomere makes to the fate map in the gastrula cannot be predicted because of variability in both the position of the future dorsoventral axis with respect to the early cleavage blastomeres and the scattering of daughter cells as the gastrula is formed. Therefore, early cell divisions of the zebrafish embryo cannot reproducibly segregate determinants of tissue fates.

Animals↗
PubMed1985

Normal fates and states of specification of different regions in the axolotl gastrula.

A fate map was constructed for four regions of the early gastrula of Ambystoma mexicanum using orthotopic grafts from donors labelled with FLDx (fluoresceinated-lysinated-dextran). The region around the animal pole gave rise to epidermis only and did not include prospective neural plate. The dorsal marginal zone contributed to cephalic endoderm and to the whole length of the axial mesoderm (notochord and somites), the lateral marginal zone to lateroventral and somitic mesoderm, and the ventral marginal zone to lateroventral mesoderm. It was found that the dorsal marginal zone contributed relatively more to the anterior regions of the mesodermal mantle and the ventral marginal zone more to its posterior parts. The same regions of the gastrula and also vegetal yolky tissue were cultured as explants and labelled with tritiated mannose. Their glycoprotein synthesis pattern was compared to those of the neurula tissues to which they contribute in vivo. Animal pole explants synthesized large amounts of the epidermis-specific marker epimucin. Dorsal marginal zone explants did not synthesize epimucin but did make amounts of S2 and S6 indicative of mesoderm, as well as the notochord-specific markers S2.2 and S3.2. Lateral marginal zone explants showed the same pattern as the dorsal marginal zone including the two notochord-specific markers, although they do not contribute to notochord in vivo. Ventral marginal zone explants were more variable in their behaviour. Yolky tissue from the vegetal hemisphere of the gastrula or the archenteron floor of the neurula synthesized mainly polydisperse material of high molecular weight rather than discrete glycoproteins. The results indicate that at the early gastrula stage states of specification exist which correspond to the three germ layers, ecto-, meso- and endoderm. The ectodermal specification of animal pole explants is quite robust and cannot easily be changed by variation of the culture conditions. However treatment with a concentrated pellet of vegetalizing factor does induce a change to mesodermal specification, which is clearly detectable in the pattern of glycoprotein synthesis. Similar inductive interactions between different regions of the early embryo are thought to occur during normal development.

Ambystoma mexicanum↗
PubMed1994

An inhibitory effect of Xenopus gastrula ectoderm on muscle cell differentiation and its role for dorsoventral patterning of mesoderm.

In Amphibia, mesoderm cells such as notochord, muscle, and blood are formed as a result of mesoderm induction, the first known inductive interaction during the embryonic development of Vertebrates. Recent evidence shows, however, that, in addition to mesoderm induction, further cell-cell interactions during gastrulation also play an important role in the differentiation of mesoderm cells in Xenopus embryos. Here we report the existence of an inhibitory effect of gastrula ectoderm on muscle cell differentiation. When placed in contact with a muscle progenitor cell population, ectoderm from the ventral side of a gastrula embryo exerts an inhibitory effect on MyoD expression and muscle differentiation. This inhibitory effect is not observed on notochord differentiation. Dorsal ectoderm shows the same extent of inhibitory effect if it is isolated at the early-gastrula stage but gradually loses its effect during gastrulation as it is induced to become neural tissue. Cell mixing experiments have shown that this inhibitory effect, clearly seen in ventral ectoderm, is not observed in ventral mesoderm cells. We propose that the ectoderm emits a signal counteracting dorsalization and the community effect and that this signal plays an important role in the correct dorsoventral patterning of mesoderm.

Animals↗
PubMed1994

Superficial cells in the early gastrula of Rana pipiens contribute to mesodermal derivatives.

We have studied the fate of presumptive mesodermal cells in the early Rana pipiens gastrula. We labeled superficial cells of the early gastrula with 125I. We also labeled all cells in a gastrula with rhodamine-lysine-dextran cell lineage tracer and superficial cells with 125I and then grafted small pieces of the marginal zone orthotopically into unlabeled host embryos. Labeled progeny were identified in sectioned embryos at the tail bud stage. The use of double-labeled grafts allowed us to study the relative contributions by superficial and deep cells to different derivatives. We found that the notochord and somite regions are both derived from the superficial and deep portions of circumblastoporal regions. In contrast, pronephros, lateral plate, cardiac anlagen, and blood cells only arise from deep cells in circumblastoporal regions. Such observations indicate that the fate map for R. pipiens is different from that of Xenopus laevis, where mesodermal derivatives appear to be restricted to the deep layers, and from that of Pleurodeles waltl, where all mesodermal derivatives are formed both from superficial and deep layers. We also have shown that from the neurula stage there is substantial mixing between cells due to the ingression of cells in the dorsal region so that superficial labeled cells, initially located in the roof of the archenteron, contribute to ventral regions of both the somites and notochord.

Animals↗
PubMed1997

The Spemann organizer of Xenopus is patterned along its anteroposterior axis at the earliest gastrula stage.

The Spemann organizer is largely responsible for organizing and patterning the anteroposterior axis during the development of amphibians. In this report, we examine the degree of anteroposterior pattern in the earliest gastrula organizer of Xenopus using a combination of embryological and molecular techniques. When we divide the earliest gastrula organizer, a region measuring 20 cells high by 25 cells wide, into stereotyped anterior (vegetal) and posterior (animal) halves, each half not only has a distinct fate and state of specification, but also induces a unique set of region-specific neural genes. When wrapped in animal cap ectoderm, the anterior half induces only anterior-specific genes (XAG-1 and otxA), while the posterior half induces anterior (otxA and reduced levels of XAG-1) and posterior (Hox B9) neural genes, revealing early localization of neural posteriorizing activity to posterior mesendoderm. This is the earliest demonstration of regionalized neural induction by the Xenopus organizer. Additionally, based on the expression of gsc, Xbra, and Xnot, we show that the organizer is patterned both at the early gastrula stage and prior to the appearance of bottle cells.

Animals↗
PubMed1999

Isolation of novel cDNAs by subtractions between the anterior mesendoderm of single mouse gastrula stage embryos.

The anterior mesendoderm of mid- to late primitive streak stage mouse embryos has the ability to induce anterior neuroectodermal fate in naive epiblast [S.-L. Ang and J. Rossant (1993) Development 118, 139-149]. A number of genes have been found to be expressed in this tissue, notably the transcription factor Lim1. Lim1-null mice have anterior mesendoderm defects that result in a lack of head formation. Thus, the anterior mesendoderm of gastrula stage mouse embryos should express Lim1-regulated genes that are essential for head development. To identify Lim1-regulated genes, a differential screen with subtraction was developed, using cDNA pools that were amplified from the anterior mesendoderm of single wild-type and Lim1-null gastrula stage embryos. This novel screen strategy has yielded 22 cDNAs that show differential expression between anterior mesendoderm cells of wild-type and Lim1-null embryos. The expression of one novel cDNA SII6 initially colocalizes with Lim1 in the anterior mesendoderm of gastrula stage embryos. Moreover, SII6 expression is undetectable in the anterior mesendoderm of Lim1-null embryos. This screen identifies a set of putative Lim1 target genes that may have important roles in vertebrate head formation. Furthermore, this differential screen strategy should provide a broadly applicable approach to identify differences in gene expression between embryonic tissues of limiting quantity.

Animals↗
PubMed2001

Boundaries and functional domains in the animal/vegetal axis of Xenopus gastrula mesoderm.

Patterning of the Xenopus gastrula marginal zone in the axis running equatorially from the Spemann organizer-the so--called "dorsal/ventral axis"--has been extensively studied. It is now evident that patterning in the animal/vegetal axis also needs to be taken into consideration. We have shown that an animal/vegetal pattern is apparent in the marginal zone by midgastrulation in the polarized expression domains of Xenopus brachyury (Xbra) and Xenopus nodal-related factor 2 (Xnr2). In this report, we have followed cells expressing Xbra in the presumptive trunk and tail at the gastrula stage, and find that they fate to presumptive somite, but not to ventrolateral mesoderm of the tailbud embryo. From this, we speculate that the boundary between the Xbra- and Xnr2-expressing cells at gastrula corresponds to a future tissue boundary. In further experiments, we show that the level of mitogen-activated protein kinase (MAPK) activation is polarized along the animal/vegetal axis, with the Xnr2-expressing cells in the vegetal marginal zone having no detectable activated MAPK. We show that inhibition of MAPK activation in Xenopus animal caps results in the conversion of Xnr2 from a dorsal mesoderm inducer to a ventral mesoderm inducer, supporting a role for Xnr2 in induction of ventral mesoderm.

Actins↗