A preliminary report on histochemography.
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Histological Techniques↗
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Sulfhydryl detection by histochemography.
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Sulfhydryl Compounds↗
Freeze-fracture-autoradiography.
A new method for the electron microscope autoradiography of soluble substances in frozen tissue is described. The basic features of the method are freeze fracturing, the application of a suitable monolayer followed by exposure at low temperature and finally the separation of tissue and the replica-monolayer-sandwich after photographic processing. The advantages and limitations of the new method are discussed in terms of monolayer quality, contact, histochemography, resolution, freezing and recrystallisation artefacts.
Animals↗