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At least 19 recordsLinked to original sources
PubMed1977

A simplified determination of the urinary luteinizing hormone surge using the hemagglutination inhibition test.

The hemagglutination-inhibition test for urinary luteinizing hormone (LH) has been modified so that the test for LH surge can be assayed in 1 day. A first morning voided urine specimen submitted to the laboratory can be analyzed in 5 hours by ordinary bench procedures. This modification involves a 20-fold reduction, as compared with the older procedure, of the duration of incubation of the urine-antiserum mixture. Another modification is the choice of aliquots which are adaptable to automatic pipetting. Only a single dilution of urine is necessary to determine LH surge for the anticipation of ovulation. Daily LH determinations on 12 normally menstruating women (av. cycle 28.18 days) reveal consistent patterns. For the 40 cycles studied LH surge occurred 14.29 days (av.) prior to the onset of the next menstruation and coincided with the rise in basal body temperature. One woman with an unusually high incidence of familial twinning showed two large LH surges in each consecutive cycle.

Adult↗
PubMed1976

Rubella antibody assay by the immunoperoxidase technique: comparison with the hemagglutination inhibition test for determination of immune status.

The rubella immune status of 128 young women was determined with two assays for rubella antibody, the immunoperoxidase technique and the hemagglutination inhibition test. Comparison of the results of these techniques showed agreement in 125 cases (110 positive and 15 negative). Positive results were obtained for one woman only by the immunoperoxidase assay and for two women only by the hemagglutination inhibition test; all three of these women had a minimal titer of rubella antibody of 1:16. The sensitivity of the immunoperoxidase test was comparable to that of the hemagglutination inhibition test, and the specificity of the former test was verified. The peroxidase-labeled antibody technique is easier to perform than the hemagglutination inhibition test and is less time-consuming since sera do not require prior treatment and results can be obtained in as little as 90 min.

Antibodies, Viral↗
PubMedDate not supplied

[The use of hemagglutination inhibition tests and complement fixation tests for evaluation of immunity states and diagnosis of recent rubella infections].

Comparative HAI and CF study of German measles antibodies in 575 sera collected from subjects of different age gave positive HAI reactions in 87% of the cases and positive CF reactions in 55% of the cases. The greater sensitivity of HAI and the longer presence of these antibodies justifies the use of this test for investigating the state of immunity in German measles. The combined use of the two tests for the diagnosis of the disease is very useful especially when the first serum sample is collected some time after the onset when the increase in serum antibody titers can only be demonstrated by CF, as HAI antibodies reach a high titer soon after the onset.

Antibodies, Viral↗
PubMed1975

Influence of albumin on rubella virus hemagglutination and the hemagglutination-inhibition test.

The HEPES-saline-albumin-gelatin (HSAG) diluent found optimal for agglutination of fowl erythrocytes by rubella virus antigen is also optimal for agglutination of trypsin-treated human group O cells. Albumins from different commercial sources, however, can have varying inhibitory effects on rubella hemagglutination titers. This can have a significant effect on the hemagglutination-inhibition test since antibody titers measured by this procedure are related to the amount of antigen used.

Animals↗
PubMed1976

Application of freeze-dried, one-day-old chick erythrocytes to viral hemagglutination and hemagglutination-inhibition tests.

Erythrocytes collected from 1-day-old chicks were stabilized by fixation with formaldehyde and by freeze-drying after treatment with carbon monoxide. Suspensions of freeze-dried erythrocytes in distilled water or physiological saline had a homogeneous bright reddish-purple color. Freeze-dried erythrocytes were compared with fresh erythrocytes for hemagglutination and hemagglutination-inhibition tests for various viruses including rubella, Japanese encephalitis, influenza, mumps, Newcastle disease, and Sendai viruses. After storage at 4 degrees C for 1 year or more, freeze-dried erythrocytes maintained their original appearance and sensitivity to hemagglutination antigens.

Animals↗
PubMed1977

Application of freeze-dried monkey erythrocytes to measles viral hemagglutination and hemagglutination-inhibition tests.

Erthrocytes collected from monkey species including grivet, rhesus, and cynomolgus monkeys were stabilized by fixation with glutaraldehyde of a low concentration and were freeze-dried in vacuo. These freeze-dried erythrocytes were compared with fresh erythrocytes for measles viral hemagglutination and hemagglutination inhibition and could be used in both tests instead of fresh erythrocytes. They maintained their initial appearance and sensitivity to measles viral hemagglutinins after storage at 4 degrees C for 4 months more.

Animals↗
PubMed1977

Comparisons of Venezuelan encephalitis virus strains by hemagglutination-inhibition tests with chicken antibodies.

Twenty strains of Venezuelan encephalitis (VE) virus inoculated intravenously in large doses into roosters produced hemagglutination-inhibition (HI) antibodies detectable in plasmas within 7 to 10 days. No signs of illness occurred, and there was no evidence of viral growth in tissues since blood concentrations of infectious virus steadily decreased after inoculation. HI antibodies in early plasmas were specific for VE virus and did not cross-react significantly with two other North American alphaviruses, eastern and western encephalitis viruses. VE virus strains could be distinquished by virus-dilution, short-incubation HI, but not by plasma-dilution neutralization tests, by using early rooster antibodies. The distinctions by HI test were similar with some strains to, but different with other strains from, those described by Young and Johnson with the spiny rat antisera used to establish their subtype classifications of VE virus (14, 28). Nevertheless, results of HI tests with rooster antibodies correlated with equine virulence, as did results with spiny rat antibodies, and distinguished the new strains of virus that appeared in Middle America during the VE outbreak of 1969 from preexisting strains.

Animals↗