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At least 19 recordsLinked to original sources
PubMed1977

Evaluation of a self-instructional unit for use in educational programs in histologic technique.

A linear programmed instructional booklet was developed and used to evaluate the acceptability of this learning strategy to histologic technique instructors and their students. Comparisons of students' cognitive knowledge of the relevant topics before and after using the booklet indicated that most were achieving the booklet's objectives. The post-test score was not significantly affected either by prior experience with programmed materials or by the way the booklet was used. The verbal attitude responses of the students were encouraging, and most indicated the booklet was an efficient way for them to learn, and it was helpful in clarifying concepts. The verbal attitudes of the instructors were also favorable. The indications were that booklet use shortened subsequent lecture time and that the booklet was most valuable as a supplement to conventional instructional methods.

Achievement↗
PubMed1977

The atrioventricular node, His bundle and bundle branches-a new histologic technique.

A technique for examination of the conducting system of the heart is described. A block of tissue embracing the ostium of the coronary sinus, the pars membranacea, the septal leaflet of the tricuspid valve, and appropriate amounts of interatrial and interventricular septum is flattened and fixed in a Kaiserling I solution. Blocks are subsequently cleared in methyl salicylate and trimmed. Sections are cut from the blocks after paraffin embedding beginning from the endocardial surfaces of the right heart chambers. The sections are mounted and stained on 35 mm unperforated leader film and covered with an acrylic preservative. For examination of the conducting system many fewer sections are required than with previous techniques.

Atrioventricular Node↗
PubMed1979

Testicular biopsy in the study of male infertility: its current usefulness, histologic techniques, and prospects for the future.

Testicular biopsy has been widely used for the diagnosis of male infertility for more than three decades. During that time, with advances in cytogenetics, radioimmunoassay, and endocrinology, the role of testicular biopsy has changed. Testicular biopsy is still useful in the diagnosis of azoospermic and oligospermic males without stigmata of gonadotropic insufficiency or Klinefelter's syndrome. A classification and description is presented of pathologic changes in the testis as seen on testicular biopsy by light microscopy. The present rationale for testicular biopsy in infertility, the processing and staining of histologic material, and the role of testicular biopsy in infertility and infertility related situations, including cryptorchidism, malignant disease, and chemotherapy related changes, are discussed. Further understanding of testicular function and disease will depend upon the correlation of histologic and ultramicroscopic changes, immunohistologic localization of hormones, and epidemiologic and endocrinologic data.

Adolescent↗
PubMed1978

[Histological technique for intramural ganglia in thick tissue preparation (author's transl)].

A simple staining technique for gastrointestinal tract neurons in Auerbach's plexus is proposed in thick tissue preparation. The ganglion cells between two muscular layers are easily seen by optical microscope examination in "spread" preparations up to 300 micron in thickness, following dehidratation. Routine paraffin sections can be used. Neurons in thicker preparations (over 300 micron) like in human small intestine can also be seen but only by using small to medium magnifications. A special fixation procedure was developed for this technique.

Adult↗
PubMed1977

Techniques for histological reconstruction of brain stem recording sites.

The masseter muscle of Sprague-Dawley rats was injected with 10 mg of horseradish peroxidase. The electrical activity of the trigeminal motor nucleus was investigated 24-36 hours later using micropipettes filled with a 2.0 M NaCl-7% fast green FCF solution. A parallel series of penetrations at 200 micron intervals was made through the motor complex at known depths. The grid formed from these penetrations was reconstructed using the following techniques. Marks were made at known depths in one or more of the tracts by iontophoresing fast green from the microelectrode tip with a hyperpolarizing current of 10 muA for 10 minutes. To assure proper alignment of the tissue containing the green marks, two agar-India ink plugs were placed caudal and parallel to the recording sites. The frozen tissue was sectioned on a microtome, first through the agar plugs and then through the tissue containing the green marks. The tissue could be incubated for the peroxidase reaction product or stained for Nissl substance. These combined procedures offer a means to correlate the structure and function of brain stem nuclear groups.

Agar↗
PubMed1979

A differential staining technique for vertebrate histology.

A staining method is described for studying micro-anatomy of different vertebrate tissues in the light microscope. A staining sequence of celestin blue--erythrosin--orange G--fast green with mordanting in phosphomolybdic acid yields a satisfactory differentiation and fine colour contrast in various tissues. The efficacy of the method was tested on different avian and mammalian tissues.

Adipose Tissue↗
PubMed1979

Methodological aspects of some inner ear vascular techniques.

Modifications of preparation technique assessing cochlear vasculature together with suggestions regarding their applicability to various types of research projects are presented. Detailed information is given regarding the 'soft-surface' dissection and preparation technique along with selected parameters for evaluating the cochlear vasculature. Some basic requirements for any histological technique studying the inner ear blood supply are suggested.

Animals↗
PubMedDate not supplied

[A useful addition to the cyto-diagnosis of hemorraegic pleural fluids: the histological examination of the clot (author's transl)].

As an addition to classical cytology, the authors propose this histological examination of the clot, which is frequently observed in pleural fluids. This clot is taken before any centrifugation which might alter the former cellular groups. This method has been undertaken on 98 cases out of 196. Only 61 clots were prepared according to the histological technique, out of which 24 were positive. The only criteria taken under consideration were the histioid, trabecular or glandular groups. This technique enables the authors to a precise diagnosis in about one third of the suspicious cases and, besides, to discover a few positive cases which had escaped the routine technique.

Blood Coagulation↗
PubMed1977

A method of morphological identification of individual neurons in the brain in vivo and after fixation.

Pyramidal neurons from layers III and V of the cat brain cortex being stained with methylene blue are revealed in vivo in specially prepared cortex specimen by means of contact micro-objectives in the reflected light. Small pieces of the brain tissue with identified neurons are excised after their fixation in situ under visual control and are placed in special grid-baskets in definite positions. Orientated and fixed by the basket's edges a bit of tissue is subjected to further histological treatment. This method permits the investigation of the same neurons after fixation by means of histological techniques suited for light or electron microscopy.

Animals↗
PubMed1976

Small-bowel abnormalities in multiple sclerosis.

Jejunal biopsies were performed in 12 randomly chosen patients with multiple sclerosis. The jejunal mucosa was examined histologically, ultrastructurally, and by tissue immune techniques. Histology showed a normal mucosa in 7 patients, increased inflammatory-cell infiltration in 3, a partial villous atrophy in 1, and a subtotal villous atrophy in the remaining patient. Fine structural abnormalities were seen in 6 of 8 patients studied. These included microvillous changes, increase in theliolymphocytes and epithelial lysosomes, thickening of the connective tissue with or without collagen fibres, and numerous macrophages containing large amounts of membrane-bound electron-dense material. The latter was seen in 5 of the 8 mucosae examined.

Adult↗
PubMed1978

[Adaptation of the deoxyglucose method to the cellular level: histologic preparation of the central nervous system for high resolution radioautography].

Vascular perfusion of all products required for primary fixation, postfixation, dehydration and embedding of nervous tissue in Epon permits radio-autographic detection of radioactivity accumulated in the central nervous system after intravenous injection of [3H]deoxyglucose. This histological technique should allow application of the deoxyglucose method at cellular if not subcellular level, since a high proportion of the tracer appears to be retained in situ in specimens adequately preserved for light and electron microscope radio-autography.

Animals↗
PubMed1979

The significance of the evolution of the cerebrospinal fluid system.

A study of the comparative morphology of the cerebrospinal fluid (CSF) system has been made in amphioxus, lamprey, dogfish, goldfish, lungfish, frog, salamander, turtle, pigeon, and mouse. Using mainly intracardiac fixation and a careful histological technique, serial sections have been obtained of the brain in situ surrounded by its various membranes and the skull. The ventricular system, the roof of the hindbrain, the meninges and subarachnoid space, the ependyma with its various derivatives, including the choroid plexuses and paraphysis, and the relationship between the various CSF compartments and the cerebrovascular system have all been compared in these animals. The hypothesis has been derived that the CSF system is primarily developed to maintain the chemical environment necessary to the function of the cells of the central nervous system, including the neuroendocrine pathways.

Animals↗