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At least 19 recordsLinked to original sources
PubMed2026

In silico genome mining and characterization of putative horse feces-derived bacterial phytases as potential monogastric animal feed additive candidates.

Phytic acid exerts a significant antinutritional effect in poultry, swine, and fish, which can be mitigated by supplementing monogastric feeds with efficient microbial phytases. Accordingly, mining bacterial genomes for novel phytases represents a strategic computational approach to identifying candidates for improving monogastric animal nutrition. In this study, 162 bacterial genomes associated with horse feces were systematically mined using an in silico pipeline to identify and characterize putative phytases.A total of 69 non-redundant sequences were identified and classified as histidine acid phytase (HAPhy) or protein tyrosine phosphatase-like phytase (PTPLPhy). HAPhys were detected in the genomes of Escherichia coli, Klebsiella pneumoniae, Salmonella enterica, Acinetobacter baumannii, and Cutibacterium equinum, whereas PTPLPhys were found in K. pneumoniae, Limosilactobacillus reuteri, Pediococcus acidilactici, Bifidobacterium pseudolongum, and Prescottella equi. Principal component analysis identified glucose-1-phosphatase (CAJ1242485.1) and bifunctional acid phosphatase (NHR17779.1) as the HAPhy candidates exhibiting the most favorable predicted physicochemical properties for potential feed applications. Similarly, among the PTPLPhys, protein tyrosine phosphatase (UNQ40438.1) and a hypothetical protein (CAJ1246072.1) showed the most favorable computational profiles. Biosafety analysis identified potential virulence factors, indicating that sources should be screened prior to feed application. High-quality AlphaFold2 models were obtained for these phytases (90.9-97.2). Molecular docking analysis showed that NHR17779.1 exhibited the strongest binding to phytic acid, whereas CAJ1246072.1 demonstrated the weakest interaction. Overall, this study identifies the horse fecal microbiota as a diverse source of putative phytases that may serve as promising targets for genetic and protein engineering; however, further in vitro and in vivo studies are essential to validate the enzymatic activity and industrial efficacy of these computational candidates.

Bacterial phytase↗
PubMed1979

Benzimidazole resistance in small strongyles of horses.

Treatments with mebendazole, cambendazole or febantel were ineffective in reducing the faecal egg count of a group of 40 horses infected with small strongyle species. Evidence was also obtained that this apparent resistance extended to fenbendazole. In horses dosed with morantel tartrate egg counts were reduced to zero by 7 days after treatment. The development of resistance is discussed in relation to previous treatment of the horses and the mode of action of febantel.

Animals↗
PubMed1979

A technique for the conduct of nutritional balance experiments in horses.

Standardbred geldings were used in a number of nutritional balance experiments. These consisted of 28-day cycles each of an 18-day equilibration period in yards and a 10-day settling and collection period in metabolism crates. The crates were made of galvanised metal tubing over wooden flooring covered by rubber matting and rubber sheets. Total faeces were collected in trays at the rear of crates and total urine by canvas funnels slung beneath the sheath of each horse.

Animal Feed↗
PubMed1979

Selective medium for Corynebacterium equi isolation.

The development of a selective medium for the isolation of Corynebacterium equi is described. The medium has been used to examine fecal samples from 127 horses of which 90 have been found to carry the organism.

Animals↗
PubMed1979

Critical tests and clinical trials on oxibendazole in horses with special reference to removal of Parascaris equorum.

The efficacy of oxibendazole given at dose level of 10 mg/kg of body weight was determined by 10 critical tests in foals and by 2 clinical trials in 20 foals (16 treated, 4 nontreated), with special interest in the drug activity against Parascaris equorum. The drug was uniformly efficacious (100%) against P equorum in the 10 critical-test foals, each having between 22 and 236 ascarids. Posttreatment reductions of ascarid egg counts in fecal samples were also 100% in suckling foals treated with oxibendazole given as a drench. Ascarid eggs did not reappear in fecal samples until the 8th week after foals were treated. A paste formulation of oxibendazole at 10 mg/kg also eliminated ascarid eggs from feces of 12 suckling foals. Strongyle EPG were markedly reduced by oxibendazole in the 10 critical-test foals and in 16 treated sucklings in the clinical trials. Egg and larval count data on foals in both the critical tests and the clinical trials also indicated oxibendazole was active against Strongyloides westeri. Untoward effects of treatment with oxibendazole were not observed.

Animals↗
PubMed1977

[Cyathostominosis: a form of severe strongylidosis (author's transl)].

A form of strongyle infection in young horses and ponies, observed during the period from November to May, is described. Large numbers of larvae of Cyathostominae in the fourth or early fifth stage of development are found to be present in the (often watery) faeces. This excretion of larvae is not affected by treatment with anthelmintics. A large number of these patients die from the sequelae of verminous enteritis.

Animals↗
PubMedDate not supplied

Transmissible drug resistance in Escherichia coli isolated from healthy dogs, cattle, sheep and horses.

The antibiotic resistance patterns and the distribution of R-factors among Escherichia coli isolated from apparently healthy dogs, cattle, sheep and horses were studied. It was found that 102 strains (16.48 per cent) of a total of 619 isolates were resistant to one or more drugs. Twenty-six per cent (27 strains) of the resistant strains were capable of transferring either a part or the entire resistance patterns to the sensitive recipient strain. These results indicates that antibiotic resistance and R-factors are not particularly widespread among animals whose diet does not cotain antimicrobial drugs.

Animals↗
PubMed1976

Lungworm infection (Dictyocaulus arnfieldi) of horses and donkeys.

Dictyocaulus arnfieldi (Cobbold 1884) infects the respiratory tract of horses, donkeys, mules, hinnies and zebra. A review of the literature has been given by Round (1972). In the western hemisphere it is popularly believed that donkeys are the natural host and that horses become infected by association with infected donkeys. There is scant documentary evidence for this and, in the Soviet Union, patent infections may reach 70 per cent without mention of donkey association (Koulikov 1935, Borovkova 1948, Akramovskii 1952a). Poynter (1963) considered the infection to be rare in the United Kingdom although cases had been reported by Fletcher (1960) and Holmes (1960). The lack of precise data on this parasite has led to the assumption that it behaves like D filaria and D viviparaus of ruminants. Studies made in the United Kingdom have shown that there are some important differences (Round, 1972).

Animals↗
PubMed1979

Probstmayria vivipara pinworms in ponies.

From 1967--1978 observations were made on the presence of the small equine pinworm, Probstmayria vivipara, in seven experimental ponies. The life cycle of this nematode is unusual in that it is endogenous with development of all stages occurring within the host's digestive tract. Initially, worms were found in the feces of four of seven ponies following treatment with thiabendazole but the infection was later transmitted to all ponies possibly via coprophagy. Still later, based on fecal and postmortem examinations, four of the seven ponies lost their pinworm burdens. At necropsy, the principal sites of infection were observed to be the cecum and right ventral colon. Despite the large number of pinworms present, clinical signs were not observed.

Animals↗
PubMed1978

Prevalence and epizootiology of equine salmonellosis.

Feces from 1,451 horses entering a veterinary hospital over a 13-month period were cultured for salmonella. A total of 46 horses (3.2%) yielded 1 or more salmonella-positive fecal cultures. Twenty horses were found to be excreting salmonella in the feces on admission, and 5 of these later had severe diarrhea associated with enteric salmonellosis. Abdominal surgery and other severe stresses were associated with all cases of severe enteric salmonellosis. Serotypes of salmonella isolated included Salmonella agona (15), S anatum (14), S typhimurium (7), S typhimurium var copenhagen (4), S infantis (2), S montevideo (1), S meleagridis (1), S drypool (1), and an unnamed Salmonella serotype (1). Seven deaths were attributed to 4 serotypes (S typhimurium, 3; S anatum, 2; S typhimurium var copenhagen, 1; and S montevideo, 1). A marked seasonal incidence is isolations was found with isolations highest in later summer to early fall and lowest in the spring. It was also found that horses can shed salmonella intermittently, and a minimal of 5 consecutive negative fecal cultures is recommended before considering a horse not to be infected with salmonella.

Animals↗
PubMed1979

Pleasure horses as a possible source of Salmonella agona.

Horses in a riding stable sporadically excreted Salmonella agona, S anatum, and S newington in the feces. The three serotypes were isolated from apparently normal horses. The horses were sampled (the number sampled varied between 10 and 21) six times throughout a 13-month period. The greatest percentage of the horses (12 of 19, or 63%) were found to be excreting salmonella in September. Among the 12 horses excreting salmonella during this month, 8 (67%) were found to be excreting S agona.

Animals↗
PubMed1979

Equine parasite control using pyrantel embonate.

Over a period of several years the use of pyrantel embonate in the control of helminth infections on three equine establishments was monitored by the examination of faecal samples collected immediately before each anthelmintic treatment. With a monthly interval between treatments for three years the faecal egg output of the horses remained at a very low level. One one establishment this was maintained when the treatment interval was extended to one-and-a-half months after treating monthly for two years. If a treatment interval of one-and-a-half months was used continuously for a number of years there was a cumulative effect, and the benefits, in terms of reduced faecal egg output of the horses, were more apparent during the second year of the control programme.

Animals↗