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At least 19 recordsLinked to original sources
PubMed1983

Monoclonal autoantibody directed toward histone and capable of inducing LE cell formation.

LE cell formation is one feature of systemic lupus erythematosus exhibited by virtually all mice of the NZB/NZW strain and is the result of accumulation of antibodies directed against components of cell nuclei. A hybrid cell line which produces antibodies capable of inducing LE cell formation in vitro has been isolated in a hybridoma fusion using the splenocytes of unimmunized NZB/NZW mice. These monoclonal autoantibodies provide an intense staining of the chromatin in cells of a number of divergent species and tissues. They bind strongly to the histone rich (2 M NaCl) fraction of extracted, isolated nuclei. Further analyses using the antibodies in immune precipitations and in antibody labeling of capillary blots on nitrocellulose sheets of calf thymus histone demonstrate that the antibodies are directed against histones and are capable of reacting with H1, H2a, H2b, H3, and H4 histones individually. In contrast to human autoantibodies with histone specificity, no cross-reactivity of this monoclonal autoantibody with the lymphocyte surface could be detected by either immunofluorescence or immunoelectron microscopy.

Animals↗
PubMed1978

Anti-DNA antibody determination in systemic lupus erythematosus (SLE). Predictive value as compared with the LE-cell test.

All pathological LE-cell tests and anti-DNA antibody determinations were registered in a prospective study over a period of 9 months at a department of internal medicine with rheumatology as a subspecialty. The results were compared with the clinical diagnosis of the patients, and correlated to SLE disease activity, as evaluated clinically and by blood analysis of five acute-phase reactants. Of 159 positive LE-cell tests, 43 were from patients with SLE, giving a diagnostic value of 27%. Of 37 positive anti-DNA antibody values, 30 were from patients having SLE, giving a diagnostic value of 81%. All of the 7 false-positive anti-DNA antibody determinations were only slightly above the normal range (22--26%: normal range less than or equal to 21%). No correlation was found between the activity of the SLE and the degree of the positive LE-cell test. Pathological levels of anti-DNA antibodies were always found when the patient had active SLE--clinically and by elevation of acute phase reactants. On the other hand, it was impossible to evaluate the disease activity from the level of the anti-DNA antibody test. It is concluded that the anti-DNA antibody determination should be preferred to the LE-cell test in the evaluation of patients with connective tissue diseases.

Antibodies↗
PubMed1978

Small inclusions in the cytoplasm of leukocytes in LE cell tests.

Small homogeneous basophilic and eosinophilic particles varying in size from 0.7 to 3.0 micra were seen in cytoplasm of monocytes and polymorphonuclear cells of leukocytes in LE cell tests. The aim of this investigation was to determine their frequency and diagnostic significance. Inclusions were found in one-third of LE cell tests on patients with rheumatic diseases and were not observed in normals. In 19.1% of 161 patients with SLE, they were present before LE cells were found. Sixty-eight% of patients who had particles had SLE, 8% had rheumatoid arthritis with LE cells, and 3% had nonrheumatic diseases.

Adult↗
PubMed2001

Novel facts about an old marker: the LE cell.

For more than fifty years, lupus erythematosus (LE) cells were believed to result from in vitro opsonization of bare nuclei by serum antinuclear antibodies and their ultimate phagocytosis by neutrophils. Twenty years ago, we described that certain antinuclear antibodies could enter into viable cells, and later on, it was proved that penetration of anti-DNA antibodies into cells results in protracted active cell death. Recent findings indicate that the material engulfed by LE cells are apoptotic blebs as residuals of cells dying after penetration of anti-DNA antibodies. These observations not only change the interpretation of the presence of the LE cell phenomenon, but also stress the potential pathophysiological role of antibodies to intracellular antigens in autoimmune diseases.

Active Transport, Cell Nucleus↗
PubMedDate not supplied

[LE cells in the synovial fluid].

The occurence of LE cells in the synovial fluids of two patients is reported. The demonostration of LE cells in synovial exudate is clinically valuable. Examination of joint fluid should therefore pay attention to the search for LE cells and their precursors. Reoccurrence of E-cells in the synovial fluid indicates their origin in the joint cavit. They could still be found even after multiple intraarticular injections of triamcinolone acetonide.

Arthritis, Rheumatoid↗
PubMed2000

'LE cells' result from phagocytosis of apoptotic bodies induced by antinuclear antibodies.

Lupus erythematosus (LE) cells are believed to represent phagocytosis by granulocytes of cell nuclei whose DNA has been 'depolymerized' and opsonized by serum factors, most likely antinuclear antibodies and C3b. Since it is known that certain antinuclear antibodies are capable of inducing apoptosis after intracellular penetration; and that the resulting apoptotic bodies can be ingested by non-professional phagocytes, we decided to investigate the possibility that LE cells could result from the phagocytosis of apoptotic bodies induced by antinuclear antibodies. We demonstrate herein, through different methodological approaches, that the ingested material within LE cells corresponds to apoptotic bodies, and that the LE cell phenomenon can be reproduced, in the absence of other serum factors, by penetrating murine monoclonal anti DNA antibodies.

Animals↗
PubMed2004

Flow cytometric analysis of the LE cell phenomenon.

A flow cytometry-based phagocytosis assay was developed and utilized to measure the LE cell phenomenon at the single cell level in vitro. Since the lupus erythematosus (LE) cell phenomenon is a special form of necro-phagocytosis in the presence of anti-dsDNA antibodies, dead substrate cells or chicken erythrocytes nuclei (CEN) served as targets that were labeled with propidiumiodide (PI). Phagocytes (PMN) were stained by anti-CD45 mAb FITC. After co-incubation phagocytosis was measured by flow cytometry. Flow cytometric analysis enabled the discrimination between PI+/CD45- targets, PI-/CD45+ phagocytes, and PI+/CD45+ phagocytes with engulfed targets. Maintaining the samples on ice significantly reduced the phagocytic uptake as compared to samples co-cultivated at 37 degrees C (p < 0.0002). The phagocytic up-take was lowest after substrate pre-treatment in normal serum as compared to samples with either no serum exposure or pre-treatment in LE-serum with anti-dsDNA antibodies (p < 0.05). Taken together, these data suggest the phagocytosis-based flow cytometry assay is suitable for analyzing the LE cell phenomenon. This method provides an interesting, simple and rapid new tool, and will possibly alleviate further studies on the LE cell phenomenon with modified cell models and/or conditions.

Animals↗
PubMed1976

[A new technic for LE cell formation. Preliminary report].

In special designed vials using coverslides glued to segments of polyethilene test tubes of 0.15 ml of fibrin free blood were incubated during 45 min to 37 degrees C in a humid chamber. Blood samples were obtained from patients with disseminated Lupus erythematosus (DLE) and normal subjects (N). Adhered polymorpho-nuclear cells (PNC) to glass were washed with Hank's solution; immediately 0.15 ml of DLE or N serum containing 5X10(6) lymphocytes (L) were added to culture cells and incubated at 37 degrees C during 30 min. Lymphocytes were previously incubated at 37 degrees C during 30 min with either N serum or DLE serum. Thereafter the segment of polyethilene test tube was detached from coverslides and cells attached to glass was washed with Hank's solution and stained with Wright solution. PMN of DLE and N in presence of L of DLE and N incubated with fresh serum of DLE formed 5 to 15% of LE cells. Determining factor for LE cells formation is the serum of DLE. Slides contained only PMN and LE cells which make easy the observation of results. All possible combinations with PMN and DLE serum and N serum allowed inclusion of several negative or positive control groups.

Adolescent↗
PubMedDate not supplied

[A case of pulmonary hemosiderosis associated with rhumatoid arthritis with LE cells (author's transl)].

In a three and a half old girl, we have noticed the apparition: first, of on idiopathic pulmonary hemosiderosis; then, five months later, of a rheumatoid arthritis with LE cells and antinuclear antibodies of very difficult detection. The meaning of this articular involvement is discussed: rheumatoid arthritis with LE cells, or rheumatoid arthritis marking the beginning of a systemic lupud erythematosus. The literature concerning the association of an hemosiderosis and a connectivite is reviewed.

Antibodies, Antinuclear↗
PubMed1967

Immunopathologic studies of systemic lupus erythematosus (SLE). I. Tissue-bound immunoglobulins in relation to serum antinuclear immunoglobulins in systemic lupus and in chronic liver disease with LE cell factor.

We studied the composition of tissue-bound immunoglobulins and of antinuclear factors by immunofluorescent techniques in five patients with systemic lupus and two with chronic liver disease associated with positive LE cell tests. Renal glomeruli in all seven demonstrated deposits of bound gammaG-globulin and complement, although the presence of gammaA- and gammaM-immunoglobulins was variable. Blood vessel walls contained primarily gammaG-globulin and complement in the systemic lupus patients, but such deposits were absent from vessels in the two with chronic liver disease. We observed antinuclear factors, demonstrated by immunofluorescence, in all three immunoglobulin classes. In six of the seven patients, evidence was obtained of a correspondence between the classes of bound immunoglobulins in glomeruli and vessels and the serum titers of antinuclear immunoglobulins. These observations are consistent with the concept that immunoglobulin deposits in tissues may be derived at least in part from antinuclear factors. Neither bound immunoglobulins nor complement was observed in liver parenchyma of the two patients with chronic liver disease or in two patients with systemic lupus and liver pathology. It thus seems doubtful that serum antibodies play a primary role in the pathogenesis of forms of chronic liver disease associated with positive LE cell tests.

Adult↗