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Results for “LHR”

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PubMed2025

Spatiotemporally resolved GPCR interactome uncovers unique mediators of receptor agonism.

Cellular signaling by membrane G protein-coupled receptors (GPCRs) is governed by a complex and diverse array of mechanisms. The dynamics of a GPCR interactome, as it evolves over time and space in response to an agonist, provide a unique perspective on pleiotropic signaling decoding and functional selectivity at the cellular level. In this study, we utilized proximity-based APEX2 proteomics to investigate the interaction network of the luteinizing hormone receptor (LHR) on a minute-to-minute timescale. We developed an analytical approach that integrates quantitative multiplexed proteomics with temporal reference profiles, creating a platform to identify the proteomic environment of APEX2-tagged LHR at the nanometer scale. LHR activity is finely regulated spatially, leading to the identification of putative interactors, including the Ras-related GTPase RAP2B, which modulate both receptor signaling and post-endocytic trafficking. This work provides a valuable resource for spatiotemporal nanodomain mapping of LHR interactors across subcellular compartments.

Humans↗

PubMed1979

Liquid holding recovery in E. coli K12. I - Substances released during buffer holding.

It is known that the incubation, in a buffer, of UV-irradiated E. coli cells results in viability increase, this phenomenon had been called liquid holding recovery (LHR). We have studied the cellular constituents release during LHR and verified that releasing rate is dose-dependent. LHR was also observed after nitrogen-mustard treatment and it is not blocked by caffeine. So, we suggest that LHR expression is not always a rec-gene dependent function and, probably, the survival increase could be explained by (a) DNA-repair, (b) reversible membrane damage and (c) cellular multiplication.

Buffers↗

PubMed1978

Effect of LRH on gonadotropin secretion in newborn male infants.

The concentrations of LH and FSH were measured in eight male newborn babies, aged 4-6 h, before and after the administration of 25 micrograms synthetic LHR. A comparison was performed with six control newborns receiving normal saline. Both LH and FSH rose significantly in all subjects after LHR administration. Their values were significantly higher than those observed during the control study. These data demonstrate sensitivity of the pituitary gonadotropes to synthetic LHR and a pubertal-type response to LH in the early hours of human life.

Follicle Stimulating Hormone↗

PubMed1978

Comparison of sensitivity and liquid holding recovery in rad mutants of Saccharomyces cerevisiae inactivated by UV and DEB.

Twenty one UV-sensitive rad mutants were tested for their sensitivity towards DEB. All mutants were more sensitive to this treatment than the wild type. Seven mutants were classified as supersensitive to DEB (rad 1-1, 2,3, 6, 15 and 18-2), while only rad2 and rad3 can be classified as supersensitive to UV. For all mutants ability for liquid holding recovery (LHR) after UV and DEB was compared. Mutants rad 1-1, 3, 5, 6, 9 and 11 differ in their response to LH after the two treatments. Survival of rad1-1 and rad3 increases signficantly during LH after DEB but not after UV exposure. In contrast rad5, 6, 11, and 22 show marked LHR after UV but no increase of survival after DEB treatment.

Butanes↗

PubMed1977

Split-dose and liquid-holding recovery after X-irradiation on diploid yeast Saccharomyces cerevisiae. I. Dependence on growth-phase.

Liquid-holding recovery (LHR) and the sparing effect of dose fractionation were investigated with 100 kVp-X-rays in diploid yeast from exponential and stationary phase. Exponential cells showed prompt and complete split-dose recovery. The ability to undergo LHR remained constant during 2-5 hours incubation after the first dose. Stationary-phase cells did not exhibit split-dose recovery (SDR) in a simple way: there was an increase in sensitivity after 3-5 hours followed by a gradual increases with time. It is suggested that stationary cells are starved of an essential co-factor which has to be synthesized de nova.

DNA Repair↗

PubMed2026

Interfollicular communication among preovulatory follicles after luteinizing hormone signaling.

Luteinizing hormone (LH) triggers the resumption of oocyte meiosis and ovulation in preovulatory ovarian follicles. These events have generally been viewed as autonomous responses occurring independently within each follicle. Here, however, we show that mouse preovulatory follicles can communicate with one another through an LH-induced paracrine signaling network. Isolated preovulatory follicles lacking LH receptors (Lhr-KO) resumed oocyte meiosis when co-cultured with LH-stimulated wildtype follicles, despite being unable to respond directly to LH. Oocytes within Lhr-KO follicles also resumed meiosis when exposed to conditioned medium from LH-treated wildtype follicles, demonstrating that diffusible factors mediate this interfollicular communication. Neutralizing antibodies against the epidermal growth factor receptor ligands epiregulin and amphiregulin inhibited the LH-induced interfollicular communication, identifying these LH-induced factors as key signaling molecules. Although epiregulin and amphiregulin are known to transmit LH signals within individual follicles, our findings indicate that they can also coordinate responses among neighboring follicles. Together, these results demonstrate that LH regulates a communication network between preovulatory follicles rather than acting solely at the level of individual follicles.

epidermal growth factor receptor↗

PubMed1976

Survival and liquid holding recovery in UV-sensitive strains of Saccharomyces cerevisiae after treatment with chemical mutagens and radiation.

Two UV-sensitive mutants of Saccharomyces cerevisiae rad 3 and rad 6 were tested for sensitivity to X-rays, MMS, EMS, HNO2 and DEB. Rad 3 mutant is more sensitive than the wild type strain only to HNO2 and DEB, while rad 6 is cross sensitive both to X-rays and all chemicals tested. Liquid holding recovery (LHR) was studied by comparison of cell survival immediately after mutagen treatment and after 5 days of storage in phosphate buffer. LH greatly increases cell survival of rad 3 mutant after DEB and slightly after EMS, MMS and HNO2, while after UV treatment LH significantly decreases survival of this mutant. LH increases survival of rad 6 mutant after exposure to UV, MMS and HNO2, but decreases survival of DEB-treated cells. Exposure of wild type strain to LH results in an increase of survival after UV, and DEB but not after MMS and HNO2. The results suggest that LHR is a strain- and mutagen-specific phenomenon and cannot be explained within the present knowledge of repair processes in yeast.

Alkylating Agents↗

PubMed1977

Split-dose and liquid-holding recovery after X-irradiation in diploid yeast Saccharomyces cerevisiae. II. Dependence of the recovery process on cellular protein metabolism.

In diploid yeast, split-dose recovery (SDR) after X-irradiation was affected, if incubation between split doses was performed in the presence of the protein-synthesis inhibitor, cycloheximide. In exponentially-growing cell-cultures, early SDR was undisturbed but complete recovery was not achieved. Concomitantly the cells show a decresing ability to perform subsequent liquid-holding recovery (LHR). In stationary-phase cell-cultures, SDR was completely suppressed. The cells show, however, recovery from potentially lethal damage in the presence of cycloheximide during incubation between the dose-fractions. The experimental results suggest that in diploid yeast SDR after X-irradiation is an enzymatic process dependent on a functioning protein metabolism.

Cell Survival↗

PubMed1978

Abnormal gonadotropin secretory responses to LRH in transsexual women after diethylstilbestrol priming.

To determine whether female transsexuals have abnormal hypothalamic-pituitary feedback control, pituitary LH and FSH secretory responses to synthetic LRH (100 micrograms iv) were measured in nine female transsexuals with normal menstrual cycles before and after a 7-day course of treatment with diethylstilbestrol (DES; 2 mg/day). Control groups included five heterosexual women and seven heterosexual men. Pituitary responses to LHR in heterosexual women studied in the early follicular phase increased markedly after DES administration and were clearly different from responses in men, which were all inhibited by DES. Responses to LRH in nine transsexual women studied in the early follicular phase differed strikingly from normal women in that gonadotropin responses were not enhanced by DES. The finding that the responses of female transsexuals to DES and LRH were intermediate between the female and the male patterns suggests that a biological abnormality accompanies the psychological abnormality in such patients.

Adult↗

PubMed1976

[Immunocytologic detection of peptides immunologically related to LRH and to SRIF in the garden dormouse under various conditions].

Immunocytologic technics have allowed us to detect appreciable and progressively increasing amounts of LRH and of SRIF in the median eminence of the dormouse during hibernation at 6 degrees C. In animals conditioned to darkness at 22 degrees C with the ovarian involution that is very important, there exists a nearly total disappearance of LRH while the amount of SRIF remains high. On the contrary permanent lighting at 22 degrees C responsible of an important reactivation of the genital sphere provoques a massive accumulation of LHR in the median eminence; SRIF is present in large amounts during the first weeks but diminishes of the experience is prolonged.

Animals↗