PubMed Health⌕ Search

SEARCH · PubMed Health

Results for “MONILIA”

Explore indexed PubMed citations for clinical trials, systematic reviews and public health research. Read source abstracts and follow each citation to its original PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 19 recordsLinked to original sources
PubMed1993

Mitochondrial DNA analysis of Exophiala moniliae.

Mitochondrial DNA (mtDNA) analysis with restriction enzymes, Hae III, Hind III and Msp I was performed in 17 Exophiala moniliae strains. The results were as follows: (1) E. moniliae could be classified into 10 types based on restriction patterns, (2) E. moniliae is suggested to be a complex organism because of extensive mtDNA polymorphism among strains like E. jeanselmei and (3) two types of E. moniliae are identical with two types of E. jeanselmei. These results suggest that E. moniliae is not genetically defined from E. jeanselmei and the taxonomical status of E. moniliae requires reevaluation.

DNA Restriction Enzymes↗
PubMed1988

Application of flow cytometry to differentiating Exophiala dermatitidis, E. moniliae and E. jeanselmei from each other.

We applied a flow cytometry apparatus (FCM) to differentiating Exophiala dermatitidis, E. moniliae and E. jeanselmei from each other. The wavelength of the argon laser emitted from the FCM was 488 nm and the aperture of nozzle from which the stream of fluid containing single cells was blown out was 100 micron. By irradiating the stream with laser by either the forward light scatter (FLS) or by the perpendicular light scatter (PLS), we were able to get two pieces of informations. Histograms displayed by the FLS indicate the cell size, while dot displays by the PLS reflect the cell structure. As a result, E. dermatitidis was clearly differentiated from either E. moniliae or E. jeanselmei by their histograms by FLS. In addition, dot displays by the PLS differentiated E. moniliae from E. jeanselmei. In conclusion, flow cytometry is available for differentiating E. dermatitidis, E. moniliae and E. jeanselmei from each other.

Cell Differentiation↗
PubMed1981

Subcutaneous phaeohyphomycosis caused by Exophiala moniliae.

The first documented case of phaeohyphomycosis in man caused by Exophiala moniliae is reported from South Australia. Study of herbarium specimens, living cultures, and other materials has revealed that E. moniliae was previously unknown in Australia.

Australia↗
PubMed1984

Phaeohyphomycosis caused by Exophiala moniliae.

We studied two cases of phaeohyphomycosis that occurred in Japan. Histopathologic examination of biopsy material showed the presence of dematiaceous catenulate fungal cells and mycelium characteristic of phaeohyphomycosis. In detailed mycologic study of the two cultures isolated from the patients we found that both formed conidiogenous cells with proximal swellings with long, neck-like extensions bearing distinct annellations and clusters of conidia at their apices. The isolates were identified as Exophiala moniliae de Hoog. These two Japanese cases are the second and third known cases of phaeohyphomycosis caused by E. moniliae and the first recorded in that country.

Antigens, Fungal↗
PubMed1986

Fractionation of the cellulolytic enzymes produced by a species of Monilia; purification and properties of an extracellular beta-D-glucosidase.

Extracellular cellulolytic enzymes produced by a species of Monilia could be fractionated by chromatography on SP-Sephadex, Con A-Sepharose, and cellobiose-Sepharose. These methods did not separate the beta-D-glucosidases (beta-D-glucoside glucohydrolases, EC 3.2.1.21) from the cellulases and xylanases within a single purification step. Fractionation by isoelectric focusing on a flat-bed granulated gel gave all of the beta-D-glucosidase activity in a single zone isoelectric at pH 8-9. The beta-D-glucosidase could be further purified to homogeneity by column isoelectric focusing at pH 8.0-10.5, and gel filtration on Biogel P-100. The purified beta-D-glucosidase showed optimal activity at pH 4-5 and 50 degrees, was isoelectric at pH 8.87, and had a molecular weight of 46,600. SDS-Polyacrylamide-gel electrophoresis demonstrated that the beta-D-glucosidase was not dissociated into subunits and, hence, consisted of a single polypeptide chain. The enzyme is considered a glycoprotein, as it binds to Con A-Sepharose. The beta-D-glucosidase hydrolyzed (1----2)-, (1----4)-, and (1----6)-beta-D-glucosidic linkages but not cellulose. Nitrophenyl beta-D-glucopyranosides and beta-D-xylopyranosides were also degraded. The beta-D-glucosidase was competitively inhibited by D-glucose (Ki 0.67 mM).

Candida↗
PubMed2003

Combinations of pulsed white light and UV-C or mild heat treatment to inactivate conidia of Botrytis cinerea and Monilia fructigena.

The use of pulses of intense white light to inactivate conidia of the fungi Botrytis cinerea and Monilia fructigena, responsible for important economical losses during postharvest storage and transport of strawberries and sweet cherries, was investigated in this study. In the first stage, a light treatment applying pulses of 30 micros at a frequency of 15 Hz was investigated, resulting in a treatment duration varying from 1 to 250 s. The conidia of both fungi showed similar behaviour to pulsed light, with a maximal inactivation of 3 and 4 log units for B. cinerea and M. fructigena, respectively. The inactivation of the conidia increased with increasing treatment intensity, but no complete inactivation was achieved. The sigmoidal inactivation pattern obtained by the pulsed light treatment was described using a modification of the model of Geeraerd et al. [Int. J. Food Microbiol. 59 (2000) 185]. Hereto, the shoulder length was incorporated explicitly and relative values for the microbial populations were used. In the second stage, combinations of light pulses and ultraviolet-C or heat were applied. The UV light used in the experiments is the short-wave band or UV-C, running from 180 to 280 nm with a peak at 254 nm (UV-B runs from 280 to 320 nm and UV-A from 320 to 380 nm). The UV-C doses were 0.025, 0.05 and 0.10 J/cm(2), and the temperatures for the thermal treatment ranged from 35 to 45 degrees C during 3-15 min. When combining UV-C and light pulses, there was an increase in inactivation for both B. cinerea and M. fructigena, and synergism was observed. There was no effect of the order of the treatments. For the heat-light pulses combination, there was a difference between both fungi. The order of the treatments was highly significant for B. cinerea, but not for M. fructigena. Combining heat and light treatments improved the inactivation, and synergism between both methods was again observed. Complete inactivation of M. fructigena conidia was obtained after, e.g., a 40-s pulsed light treatment and 15 min at 41 degrees C, or after an 80-s light treatment and 10 min at 41 degrees C.

Botrytis↗
PubMed2000

[Ultrastructure and mechanism of separation of macroconidia in Monilia fructigena Pers].

Monilia fructigena macroconidia are characterized by availability of endoplasmic reticulum cisternae which are arranged parallel to one another, and large globules of polyphosphates reserve in vacuoles. During the liberation of macroconidia the following processes occur: plugging of the septal pore by plug, development of second continuous septum, rupture of the outer electron dense layer of cell wall, and strict separation.

Candida↗
PubMed1996

Peritonitis caused by Monilia sitophila in a patient undergoing peritoneal dialysis.

Fungi have become an increasingly important cause of peritonitis in patients undergoing continuous ambulatory peritoneal dialysis. The most common cause of fungal peritonitis is Candida. However, in recent years unusual and "nonpathogenic" fungi have been reported as etiologic agents of CAPD-associated peritonitis. We are reporting the first case of CAPD-associated peritonitis caused by Monilia sitophila. This organism had previously been considered to be non-pathogenic, and a troublesome laboratory contaminant. Our patient was successfully managed with intravenous and intraperitoneal amphotericin B, followed by oral itraconazole, without removal of her Tenckhoff catheter.

Administration, Oral↗