Tolypocladium cylindrosporum (Deuteromycotina: Moniliales), a fungal pathogen of the mosquito Aedes australis. I. Influence of temperature, pH and salinity on the growth and sporulation of the fungus in the laboratory.
The New Zealand strain of Tolypocladium cylindrosporum was cultured on Sabouraud dextrose agar medium under varying regimes of growth conditions. The isolate exhibited good tolerances to temperature (4-35 degrees C), pH (3-10) and salinity (0-7% NaCl). Optimal vegetative growth and sporulation were recorded between a temperature range of 20-30 degrees C, pH of 5-6 and a salinity level of 0-2% NaCl. The North American isolate of the fungus showed similar tolerances, while the European isolate was less tolerant.