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Ultrastructure of testes and excurrent ducts in the bonnet monkey (Macaca radiata).

The testes and excurrent ducts of four bonnet macaques (Macaca radiata) were examined using scanning and transmission electron microscopy. The three layers of contractile and connective tissue composing the tunica albuginea, and the multilayered tunica propria of the seminiferous tubules were similar to that of man. Spermatids project from the epithelium or are found free in the lumen. The stages of the cycle of the germinal epithelium in the bonnet macaque were similar to that in other macaques. The epithelium of the rete testis consisted of thin brush-bordered cells, whereas the epithelium of the efferent ducts consisted of tall, prismatic or cylindrical cells lined by kinocillia or short stereocilia and basal cells. The height of the epididymal epithelium and lumen diameter was maximal in the caput region and minimal at the cauda. Spermatozoa were found among clusters of stereocilia. The epithelium of the vas deferens showed gradual transition from the stereocilia covering typical of the epididymis to that of a shorter, apical microvilli covering.

Animals

Passive immunization with an antibody to the beta-subunit of ovine luteinizing hormone as a method of early abortion--a feasibility study in monkeys (Macaca radiata).

Antiserum to the beta-subunit of ovine luteinizing hormone (oLH-beta) raised in monkeys (Macaca radiata) has been tested by a variety of criteria both in vivo and in vitro to establish its ability to neutralize oLH, hLH, and human chorionic gonadotropin (hCG). Passive administration of this antiserum caused inhibition of ovulation and termination of pregnancy in recipient monkeys as indicated by premature vaginal bleeding and a significant reduction in serum progesterone and estrogen levels. The results suggest that antiserum raised in monkeys against oLH-beta can neutralize monkey LH as well as monkey CG.

Abortion, Induced

Purification, properties, and partial structure elucidation of a high-molecular-weight glycoprotein from cervical mucus of the bonnet monkey (Macaca radiata).

A high-molecular-weight glycoprotein has been purified from the cervical mucus of the bonnet monkey (Macaca radiata). The glycoprotein was shown to be homogeneous by electrophoresis, sedimentation equilibrium, and N-terminal group determination, and to contain 19% protein, 19% D-galactose, 18% N-acetyl-D-galactosamine, 15% N-acetyl-D-glucosamine, 11% L-fucose, 10% sialic acid, and 1% sulfate groups, corresponding to about 1800 amino acid residues and 400 carbohydrate side chains of about 9 monosaccharides. The carbohydrate chains are linked to the peptide backbone through N-acetyl-D-galactosamine and serine (or threonine) residues. Reduction with dithiothreitol and alkylation with iodoacetic acid reduced the molecular mass from 1 to 0.5 X 10(6) daltons and produced subunits having the same size, charge, and N-terminal amino acid. Electrophoretic studies suggested the presence of disulfide bonds between two chains of the glycoprotein. Degradation with alkaline borohydride gave, after fractionation on Bio-Gel P-2, fractions containing L-fucose, D-galactose, N-acetyl-D-galactosaminitol, N-acetyl-D-galactosamine, N-acetyl-D-glucosamine, and sialic acid in the ratio of 1.0:3.0:1.0:1.0:1.3:1.0. Further fractionation by electrophoresis and paper chromatography gave a charged fraction representing 13% of the original glycoprotein. Enzymic degradation and methylation studies indicated the presence of the structure alpha-Gal-(1 leads to 3)-[Fuc(1 leads to 2)]-Gal-(1 leads to 4)-GlcNAc, linked to a core component containing N-acetyl-D-galactosaminitol.

Amino Acid Sequence

Spermiation and epididymal maturation of spermatozoa in the bonnet macaque (Macaca radiata) as viewed by scanning electron microscopy.

Spermiation and epididymal maturation of spermatozoa were studied in the bonnet monkey (Macaca radiata) by light and scanning electron microscopy. The morphology, location, and release of residual body were observed during spermiation. Along the epididymal duct, the shape of spermatozoa changed, the constriction at the anulus disappeared, the marginal thickening diminished in length, and the cytoplasmic droplet regressed and moved toward the posterior end of the middle piece. Mature spermatozoa were very similar to those of other Cercopithecidae, and they showed a forward motility and a drop in eosin stainability.

Animals

Blood groups of bonnet macaques (Macaca radiata), with a brief introduction to seroprimatology.

The human-type A-B-O blood groups of 52 bonnet macaques (Macaca radiata) were determined. Application of method of population genetics indicated the gene frequences to be 0 = 0.173, a = 0.480 and B = 0.347. Cross testing of sera and red cells of the bonnet macaques revealed two blood-type-specific isoagglutinins, one of them strong enough for use as a blood typing reagent. No blood group polymorphism was revealed by testing bonnet macaque red cells with isoantisera produced in rhesus monkeys (M. mulatta) and in crab-eating macaques (M. fascicularis). The rhesus and crab-eating macaque isoantisera reacted either with all or with none of the bonnet macaque red cells tested.

ABO Blood-Group System

An epidemiological study of neonatal and postneonatal mortality in Macaca radiata at the California Primate Research Center (1966-1973).

This study evaluates neonatal and postneonatal survival of Macaca radiata to 180 days of age at the California Primate Research Center from 1966 through 1973. Of a total of 287 live births, infant mortality rates (IMR) for three different types of housing were significantly different: 12% for inside cages, 41% for an outside one-half-acre enclosure and 31% for outside corncrib cages. Experience of the female in raising an infant to weaning age was a significant determinant only for the outside housing IMR. Introduction of laboratory-reared females into the breeding colony made the maternal breeding experience an important factor.

Animals