Convenient, simplified preparation of less commonly used media.
A method is described for the preparation of some less commonly used media. This approach should encourage use of these media.
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A method is described for the preparation of some less commonly used media. This approach should encourage use of these media.
A new rapid method for testing the growth activity of tissue culture media using the 'Raji' and 'Simpson' strains of lymphoblastoid cells grown in suspension is described. This method has advantages for rapidly assessing the quality of a culture medium.
Adequate sleep is essential for child and adolescent development, driving extensive research across scientific disciplines. This umbrella review provides a comprehensive overview of existing evidence on media consumption and sleep and thereby lays the foundation for identifying key concepts and gaps. An inclusive systematic search for reviews reporting literature searches was conducted in 02/2021 and updated last in 09/2024. Methodological quality of the included reviews was assessed using AMSTAR-2. We included 84 reviews reporting on the association between media use and sleep in individuals aged 0-18 years. The field is dominated by reviews of low methodological quality, mainly including original cross-sectional studies with subjective measures in older children and adolescents. A total of 475 original articles were covered by the reviews; only 10 of them appeared in at least seven and at most nine reviews. Screen time generally had a negative impact on sleep, though evidence varied from very low to strong. Evidence for effects of conventional books on sleep remains inconclusive. High quality systematic reviews are needed to evaluate robust studies using objective measures of sleep and contemporary media use across all age groups up to adolescence and to explore the impact of non-digital media use on sleep, considering age and gender differences.
In cooperation of nine laboratories a modified Ogawa medium containing asparagine was compared with the media used in the GDR for culture investigations on tuberculosis in routine [German Pharmacopoeia (diagnostic laboratory methods) GDR]. The efficiency of the modified Ogawa medium containing asparagine instead of sodiumglutamate was comparable to that of the original Ogawa medium and did not show any better results. By this means it is once more evident that Ogawa medium is an efficient medium for culture on tuberculosis. It is less liable to contamination and easily to be prepared.
Four interface media were tested with an electric pulp tester to determine whether the amount of current needed to elicit a response varies with the material. Each material was tested on the facial and linqual surfaces of anterior teeth. Clinical results showed no appreciable difference between the materials used, provided they were water or petroleum based. Facial and lingual readings were the same in about half the tests, and lingual readings were slightly lower than facial readings in 40% of the tests.
The accumulation of free radicals correlates with an increase in the percentage of killed lyophilized spores of actinomycetes. Therefore, the EPR method in combination with the method of "accelerated storage" at 37 degrees C was used to evaluate rapidly (during one month) the effectiveness and to select certain protective media for stabilization of actinomycetes cultures. The EPR method can be applied for comparative evaluation of only those media which did not contain originally free radicals.
By using an appropriate ratio of carbon source to mineral components, we obtained comparable citrate yields in media containing different concentrations of glucose. The enzyme system of inoculum passed on gradually from "growth" state to "production" state during the mould growth. In the starch medium, the critical factors of citric acid production are the aeration efficiency of the medium and the amylase formation of the strain. The air interruption exhibited a prolonged inhibition of the production rate but not of the citrate yield in glucose medium while those parameters in starch medium containing excessive urea were briefly but severely inhibited. After being affected by these unfavorable conditions, the production activity of Aspergillus niger could be restored by applying an appropriate fermentation process.
The effects of five iodinated contrast media and of physiologic saline on bladder capacity were studied. It was found that a significantly greater volume of sodium chloride was tolerated than of Cysto-Conray, Conray 30. Hypaque meglumine 30, Hypaque sodium, and Dimer X. Dimer X was tolerated in the smallest volume. The reason for the difference in volume tolerated is not known. The bladder mucosa showed dehydration changes 6 hr after saline cystogram with no change at 24 hr, 48 hr, 7 days, and 3 months. The contrast media produced dehydration and hydropic and desquamative changes in the bladder mucosa with at least one of these changes persisting up to 3 months. There was no significant difference in the severity of the changes produced by the various contrast media. No evidence of inflammatory changes was seen in the bladder mucosa or wall or in the ureter with any of the agents.
The value of RVA, N-1, 7H10, 7H11 and Sauton's media for studies on mycobacteriophage infeciton and lysis of mycobacteria was assessed. Experiments were made with mycobacteriophages BGI, BKI, CRI-3, G37, and LG, all of which lyse Mycobacterium smegmatis strain 607B, and with mycobacteriophage DS6A which lyses Mycobacterium tuberculosis strain H37Rv. The methods involved "direct lysis", the measurement of "routine test dilutions" and counts of plaque-forming units. It was found that N-1, 7H10 and 7H11 media gave better overall results than RVA medium for M. smegmatis strain 607B and its phages, and that RVA medium was generally the most useful for M. tuberculositems employed.
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Dissolution rates of salicylamide in water and caffeine solutions under perfect sink conditions were predicted by theoretical diffusion equations applicable to dissolution in complexing media. Experimental dissolution rates were measured using a compartmentalized rotating-basket apparatus under two sets of conditions. Agreement was found between experimental and predicted rates. Use of the theoretical equation for estimating dissolution rates involves simple calculations of diffusion coefficients and diffusion layer thickness under the operative dissolution conditions. The increase in dissolution rate caused by addition of the complexant can be calculated for diffusion-controlled dissolution directly if the stability constant and the drug solubility in water are known or measured.
"Sharenting," the practice of parents posting photographs, videos, and information about their children on social media, has an ever-growing presence in modern society. However, researchers and the public are now recognizing the potential consequences of sharing, including creation of permanent digital footprints, strained familial relationships, and threats to children's safety. Despite this emerging evidence, no U.S. clinical or legal guidelines exist for parents on safe sharing. Visits with behavioral health providers and family physicians can serve as key points for intervention. This column aims to provide clinicians with a better understanding of sharenting and its potential effects on patients and families, guidance on discussing safe online sharing, and a tool for parents and other caregivers to use when deciding whether to post.
Inocula of polluted water naturally infected with salmonellas effectively distinguished six brands of selenite and six brands of tetrathionate enrichment media into satisfactory and unsatisfactory categories. Minimal inocula of pure cultures differentiated the tetrathionates, but not the selenites. Inocula of naturally infected chicken giblets suggested that there was a difference between two comparable brands of tetrathionate, but this was not statistically significant. The difference was, however, clearly demonstrated by minimal inocula of pure cultures. Intensive investigation of two inferior tetrathionates revealed inhomogeneity in the distribution of brilliant green in one bottle of one brand. The importance of the salmonella serotype and even the colonial variant used for the pure culture inoculum was also demonstrated.
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Explore the source record for details and available documents.
Explore the source record for details and available documents.
For a comparative study on the merits of Selenit broth and Enterobacteriaceae-enrichment broth (EE-broth) by MOSSEL, the media were inoculated with 629 faecal cultures obtained from Salmonella carriers and contacts of a Salmonellosis sufferer. The Selenit broth incubated at 37 degrees C showed better results than the EE-broth incubated at 30 degrees C. After 24 hrs incubation plating was done on XLD-, SS-, and MACCONKEY-agarplates with the result that the XLD plate gave the highest yield of 21% isolations, followed by the SS-plate showing 17%, whilst the MACCONKEY-plate produced only 7%. In our routine laboratory the XLD-plate has now superseded the LEIFSON-plate and is used in parallel to the SS-plate after enrichment in Selenit-broth.