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[French evaluation of cardiac stimulation].

A national enquiry into the problems related to definitive cardiac pacemakers, carried out in 1975, has yielded certain essential findings: the number of first-time implantations of pacemakers has been increasing by about 20% per annum; 92% of electrodes are currently implanted by an endocavitary technique, thoracotomy having now practically been abandoned; 90% of pacemakers implanted in 1975 were threshold models, inhibited by a QRS complex; the indications have become progressively wider, and are essentially related with the various forms of bradycardia, most frequently those due to atrio--ventricular block. In 1976, we have now reached a figure of about 200 new implantations of pacemakers per million inhabitants; those using lithium are increasingly superceding the mercury and isotope models.

Aged

Brain tumor-scanning agents compared in an animal model.

Sixteen radiopharmaceuticals for brain tumor localization have been compared in a mouse brain tumor model. A rating system is presented for such intercomparison. The rusults indicate that 111-in-chloride injected at pH 1.5 has the most favorable biologic characterisTICS FOR BRAIN TUMOR IMAGING.

Animals

[Evaluation of gammagraphy in the differential diagnosis of cysts and renal tumors].

In view of the problems posed by the diagnosis of intrarenal masses, the authors review 16 cases of patients with this pathology who have undergone renographic and gammagraphic studies. They obtain a high level of coincidence between the gammagraphic diagnosis and its subsequent surgical confirmation, a fact which indicates that the kidney gammagraph is of great diagnostic value. This may be due to the different vascularization of the pathological area which would allow for a greater indication by the tracer, rejecting the theory of parenchymatous intoxication by toxic tumour substances.

Diagnosis, Differential

Photolysis in 131I, 35S or 14C labeled HgLSCN powders.

In 1938, S.V. Raghava Rao reported that a crystal of HgXSCN (X=halogen) has photropic properties and in 1955, the author reported the mechanism of the phototropy is identical with that of HgI2.2HgS. However, a behavior of S, I and C atoms in the crystals irradiated by sunlight was not known. Purpose of this paper is to clarify the behavior of these atoms by using radioactive tracer technique. The results obtained show that when electrons belonging to S2- or I- ions absorb photons and are excited to upper state, these electrons leave behind neutral S or I atoms, which diffuse thermally toward the crystal surface and escape into atmosphere. 14C atoms do not escape. S atoms are more sensitive to light than I or C atoms.

Carbon Radioisotopes

Labeling human lymphocytes with mercury-197.

A simple method for labeling human lymphocytes with Hg-197 is described. Lymphocytes were isolated from human blood and purified by the Ficoll-Hypaque method. Labeling was carried out by incubating viable cells suspended in saline with Hg-197 for different time intervals and at different temperatures. The labeling yield varied from 48% for 1 million cells to about 70% for 10 million cells after incubation with 2 muCi of Hg-197 for 2 hr at 37 degrees C. The temperature and incubation period beyond 1 hr had no appreciable effect on the labeling yield. The yield increased by a factor of 1.5 when the cells were stimulated by phytohemagglutinin. Subcellular distribution studies showed that 60% of the tracer localized in the nucleus and cell membrane, while the remaining portion concentrated in the mitochondrial and microsomal fractions.

Humans

Labelling of bleomycin with cobalt-57, indium-111, technetium-99m, mercury-197, lead-203, and copper-67.

The radiochemical purity of the cobalt-57 complex of bleomycin could be enhanced by adjusting the pH of the final product to a value between 5 and 6. This radiopharmaceutical appeared to have better tumor visualizing properties compared to the not neutralized preparation. The clinical use of the cobalt-57 bleomycin complex is however limited by the long physical half-life of the label, causing a risk of radioactive contamination. It appeared to be possible to label bleomycin with radioactive cations (111In3+, 99mTc4+, 197Hg2+ and 67Cu2+) having suitable gamma ray energies and short half-lifes. These bleomycin complexes showed a high radio-chemical purity judged by their behaviour on thin layer chromatography, paper chromatography, and electrophoresis, but their application as tumor visualizing radiopharmaceutical turned out to be disappointing compared with cobalt-57 bleomycin.

Bleomycin

[Management of patients after pacemaker implanation (author's transl)].

Although pacemaker therapy is characterized by a high degree of reliability, follow-up of pacemaker patients is necessary to provide early detection of pacemaker failure and optimal setting of programable pacemakers as well as to render adequate treatment of underlying disease. The most common cause of pacemaker failure is battery depletion. In spite of the available data on the mean life-expectancy of the various batteries, the individual time of depletion cannot be predicted with accuracy. Furthermore, a defect in the electronic circuitry and/or electrode may still be rarely encountered even though technical standards are high and, occasionally, threshold elevation or lead dislocation may occur in spite of conscientious implantation technique. For patients with programable pacemakers, follow-up monitoring also enables adjustment for optimal pacemaker function. Thus, through lowering of the current or narrowing of the pulse width, the life of the battery can be prolonged or, on incrementation of these 2 parameters, an increasing threshold can be compensated for within certain limits. More important, however, is the possibility of inductive pacemaker rate changes through external programing to yield the most favorable pulse interval with respect to the underlying disease. Since, in addition to impairment of the cardiac conduction system, other disease processes are frequently presented--approximately one in three patients has coronary artery disease and almost half of the patients have congestive heart failure--follow-up visits not only serve the purpose of monitoring pacemaker function but also provide an opportunity to insure an optimal clinical condition of the patient. Accurate interpretation of pacemaker function prerequisites knowledge of the type of stimulation (fixed rate, synchronized, hysteresis, magnet rate, interference frequencies), of the battery (mercury-zinc, lihium, nickel-cadmium, isotope), of the programability (rate, current, pulse width) as well as the location of impulse capture and detection and through ascertainment of secured position of the lead and generator. While the pacemaker rate enables interpretation of the state of charge of the battery, evaluation of capture and tracing threshold permit assessment of the presence of a threshold elevation, lead dislocation, an electrode fracture or a defect in the electronic circuity. Stimulation of extracardiac muscle areas may be indicative of improper positioning and inadequate isolation of the generator, lead dislocation or lead perforation.

Arrhythmias, Cardiac

Myocardial infarct imaging agents. III. Synthesis and evaluation of [203Hg] hydroxymercuriphthaleins.

Four Hg-203-mercurated phthaleins were prepared, purified, and compared with [203Hg] mercuric nitrate, [3H] phenolphthalein [203Hg] hydroxymercurifluorescein and Tc-99m-pyrophosphate in a rat model of myocardial necrosis to determine their specificities for damaged myocardium. The ratios of damaged myocardium to normal myocardium, and to blood, for the [203Hg] hydroxymercuriphthaleins (20.7-34.1 and 12.1-20.1, respectively) were somewhat lower than those reported previously for [203Hg] hydroxymercurifluorescein, but were higher than those found with [203Hg] mercuric nitrate, [3H] phenolphthalein, and Tc-99m pyrophosphate. Both the hydroxymercuri- functional group and the phthalein moiety are required for selectivity. The removal of the oxygen bridge present in fluorescein, and the replacement of carboxylic acid by sulfonic acid, had no significant effects on the sequestration process in damaged tissue.

Animals

[Multistage studies aimed at early detection of chronic effects of vinyl chloride and mercury vapors].

168 persons occupationally exposed to vinyl chloride and 236 persons exposed to mercury in plants electo two-stage medical and laboratory examinations. Workers exposed to vinyl chloride in average concentrations of 133 mg/m3 were found to show megalohepatia and abnormal enzymatic tests results (transaminic, A1AT, AP). The frequency of disturbances in workers exposed to vinyl chloride, average concentration 17.9 mg/m3, was considerably lesser. The second stage of studies involved those exposed to vinyl chloride for more than 10 years, with megalohepatia and at least 1 abnormal enzymatic test. No angiosarcoma was found. An analysis of the diagnostic value of biological tests used, indicated a great usability of liver scintigraphy and vascular system examinations for early diagnosis of vinyl chloride intoxications. In the plant where workers were exposed to mercury the Hg concentrations were found to range from 0.05 to 0.07 mg/m3. No significant differences between these workers and the control group were found, in respect to medical and laboratory examinations. The second stage of studies involved 118 persons exposed to mercury in concentrations above 20 mg/m3 of air, no matter how long the exposure lasted. The second stage of studies consisted of extensive biochemical studies, examinations of the liver using isotopic scintigraphy, psychological, psychiatric examinations and encephalographic determinations. Psychological, psychiatric and encephalographic examinations were said to be of great importance in early diagnosis of chronic mercury intoxications.

Air

Cardiac pacing.

The basic electrophysiology of temporary and permanent cardiac pacing is reviewed, as are the indications, the types of pacing systems, and the methods of implantation. Recent developments in power sources and leads are described. The mercury-zinc battery is now obsolete and is being replaced by lithium, rechargeable, and isotopic power systems. While ventricular pacing continues to be the standard, a brief review of atrial programmed systems is given, including atrial pacing and atrioventricular synchronized and atrioventricular sequential pacing. Conventional pacing is aimed at the control of symptomatic bradycardia. Brief reference is made to experimental pacing systems designed to control ventricular or supraventricular tachyarrhythmias.

Arrhythmias, Cardiac

Retention of the metabolized trace elements in biological tissues following different drying procedures. I. Antimony, cobalt, iodine, mercury, selenium and zinc in rat tissues.

Loss of Sb, Co, I, Hg, Se and Zn during freeze-drying and oven-drying at 80, 105 and 120 degrees C were studied in rat tissues that contained metabolized radioactive isotopes. No loss was observed for any of the 6 elements on freeze-drying. However, tissue-specific differences were observed in many cases for Hg, Se, I and Sb on oven-drying. A significant loss of Hg was observed in liver even at 80 degrees C, and for brain at 105 degrees C. Se was lost from whole blood, brain, lung, and muscle at 120 degrees C. Sb was lost from whole blood at 105 degrees C, but from brain, kidney, lung and spleen at 120 degrees C. Iodine was also lost from whole blood, kidney, blood serum, erythrocytes, brain, lung and muscle at 120 degrees C. Although the losses were statistically significant, they remained in most cases between 2 and 10%, with the exception of Hg at 120 degrees C, where the losses in some of the tissues were unpredictable. For urine, freeze-drying and oven-drying at 80 degrees C was found to be relatively safe for Hg and I. At 105 degrees C and above, serious loss of Hg was observed. In this experiment, the elements Zn, Co, Sb and Se were no studied for urine.

Animals

Skin perfusion pressure measured as the external pressure required to stop isotope washout. Methodological considerations and normal values on the legs.

The skin perfusion pressure was measured as the external pressure required to stop blood flow, as evidenced by cessation of the washout of an intracutaneous depot of [131I-]antipyrine mixed with histamine. The external pressure on the skin was measured by an 11 x 11 cm slack air-filled plastic cushion connected to a mercury manometer and interposed between the labelled area and a blood pressure cuff. The 'flow cessation external pressure' (FCEP) varied parallel to the intra-arterial femoral mean blood pressure, but was on the average 10.8 mmHg (SD 6.4) lower. FCEP was measured on twenty normal subjects at four different segments of the leg. The average differences between auscultatory brachial mean blood pressure and FCEP were: thigh 12.0 mmHg (SD 7.6); calf 10.4 mmHg (SD 7.4); ankle 12.9 mmHG (SD 10.1); foot 20.2 mmHg (SD 12.1). The SD of the difference between measurements on two different days, performed in forty-four patients with different degrees of occlusive arterial disease, varied between 5.6 and 8.3 mmHg at the different levels. The present data indicate that the skin perfusion pressure on the legs in normal subjects lies approximately 10 mmHg lower than the systemic mean arterial blood pressure.

Arterial Occlusive Diseases

Heavy metal-nucleoside interactions. Binding of methylmercury(II) to inosine and catalysis of the isotopic exchange of the C-8 hydrogen studied by 1-H nuclear magnetic resonance and raman difference spectrophotometry.

Raman difference spectrophotometry reveals that CH3HgII binds quantitatively to N(1) of inosine at pH 8, substituting for the proton. When N(1) is saturated, binding occurs at a second site. Measurements of the 1-H nuclear magnetic resonance spectra of both inosine and of CH3Hg-II are in agreement with the N(1) binding and indicate that the second site for mercuriation is N(7). This second binding reaction is observed to increase the rate of exchange of the C(8) hydrogen with solvent, consistent with results observed for alkylation at N(7). Coordination of the electrophilic CH3Hg-II to N(7) increases the acidity of H(8), facilitating OHminus--catalyzed proton abstraction and reprotonation by themedium. For comparison, the reaction of CH3Hg-II with [8-2-H]inosine has been studied. Displacement of the N(1) hydrogen upon mercuriation of inosine causes a significant electron delocalization into the ring, increasing the basicity of N(7), and accounting for the synergic effect in metal binding observed originally by Simpson. In contrast, 1-methylinosine interacts only slightly with CH3Hg-II at pH 8. Coordination appears to be at N(7), since H(8) again is observed to exchange rapidly with solvent protons. In acidic solution, pH less than 2, binding to inosine is almost quantitative and exclusively to N(7). The behavior of CH3Hg-II is compared with that of Pt(II) and with Ni(II), Co(II), AND Zn(II). A brief comparison is made among ultraviolet absorption spectrophotometry, nuclear magnetic resonance (NMR), and Raman difference spectrophotometry for studying reactions of nucleosides and nucleotides.

Binding Sites