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Results for “Methylcellulose”

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At least 19 recordsLinked to original sources

PubMed1979

[Coating of artificial lens with methylcellulose (author's transl)].

The corneal haze which in the past often followed lens implantation was clearly decreased by applying one drop of a 1% solution of methylcellulose into the lens immediately prior to its insertion. It is surmised that by coating the lens in this way injury to the corneal endothelium is prevented. Methylcellulose caused little if any rise of intraocular pressure postoperatively.--The preparation of a sterile solution of methylcellulose is described in detail. Finally, it is important to stress that--in addition to applying methylcellulose--one should always attempt to work under an air bubble.

Cataract Extraction↗

PubMed1976

Biodegradability of [14C]methylcellulose by activated sludge.

Three Methocel methylcellulose ethers of 1.9 degree of substitution with [14C]methyl labels were shown to be biodegradable using batch-type activated sludge tests. The maximum rate for conversion to 14CO2, attained after 1 week, was only 0.62 mg of methylcellulose/g of mixed liquor volatile solids per day. In 20 days, 55 to 73% of the radioactivity had been removed from solution as 14CO2, and the suspended solids contained 12 to 15% of the original radioactivity. Only 4% of the original methylcellulose appeared to be polymeric after the 20-day period. Thin-layer chromatography of supernatant liquid indicated at least two degradation products.

Bacteria↗

PubMed1978

Biochemical effects of gum arabic, gum tragacanth, methylcellulose and carboxymethylcellulose-Na in rat heart and liver.

Repeated oral administration of commonly used suspending media, gum arabic, gum tragacanth, methylcellulose, and carboxymethylcellulose-Na to rats caused uncoupling of oxidative phosphorylation in liver and heart mitochondria and partial inhibition of mixed function oxidases of liver endoplasmic reticulum, as measured by 2-biphenylhydroxylation and 4-biphenylhydroxylation. There were considerable differences between the compounds with regard to potency and reversibility of these effects. Only methylcellulose at a concentration of 0.5% did not alter mitochondrial function and mixed function oxidases. It is recommended as suspending medium for the use in pharmacological and toxicological experiments.

Acacia↗

PubMedDate not supplied

[Effect of methylcellulose on protein hydrolysis by pepsin in butter cream].

The digestiveability of proteins with pepsin in butter creames, where the source of nutrients formed condensed milk, was studied. It was made certain that in specimens containing a greater proportion of butter the proteins were less susceptible to be assailed. When some of the butter is replaced with methylcellulose for the purpose of reducing the calorific value of the cream there was observed an accelerated proteolysis by comparison with both the traditional specimens and those containing the same amount of fat as the test samples. In the test conditions the slowing down of the fat proteins hydrolysis was not associated with inactivation of pepsin. The cited data support the expediency of using methylcellulose in the confectionary industry.

Butter↗

PubMedDate not supplied

Recovery of Clostridium thermosulfurogenes produced beta-amylase by (hydroxypropyl)methylcellulose partition.

A procedure for recovering Clostridium thermosulfurogenes produced beta-amylase from fermentation broth by partition was developed. The partition was achieved by addition of ammonium sulfate to an aqueous solution of the enzyme with (hydroxypropyl)methylcellulose. The beta-amylase-containing pellet formed upon centrifugation could be redissolved and the polymer recovered by a second salt addition. The process was not dependent on polymer/enzyme solution pH, but it was affected by temperature, polymer nominal molecular weight and loading, and fermentation carbon source. Unlike more traditional aqueous-phase partitions, such as poly(ethylene glycol)/dextran, the current approach appeared to be biospecific.

Cellulose↗

PubMed1977

Effect of methylcellulose injection on murine hematopoiesis.

This study was designed to determine the effect of methylcellulose (MC)-induced reticuloendothelial (RE) hypertrophy on neutrophils and hematopoietic stem cells and to contrast its overall hematologic effect in the mouse to the more frequently studied rat model. Mice were given MC 3 times/wk and studies were done at 2, 3, and 4 wk, with maximal hematologic change by 2 wk. A stable, but incompletely compensated hemolytic anemia developed which was accompanied by a significant shift of erythropoiesis from marrow to spleen. Thrombocytopenia developed as did neutrophilia, accompanied by an increased number of marrow neutrophil precursors. Extramedullary hematopoiesis was observed in the liver. The number of cells forming spleen colonies in irradiated recipients increased in the spleen but not in marrow. The number of cells producing granulocyte and macrophage colonies in semisolid media increased in spleen and marrow. Splenectomized mice, treated with MC, developed changes very similar to intact mice. Thus, it appears that all three major hematopoietic lines may be destroyed by the MC-hypertrophied RE system. The mouse differs from the rat in its hematologic response to MC by destroying cells in organs other than the spleen, by increasing neutrophil production, by developing hepatic hematopoiesis, and by developing all changes more rapidly.

Anemia, Hemolytic↗

PubMed1992

[Generation of monoclonal antibodies (McAbs) against human luteinizing hormone (hLH) in a methylcellulose semi-solid medium].

McAbs against hLH were generated by a facile hybridoma technique according to the methods of Davis and Lee, et al. After routine immunization and cell fusion, the cells were plated in a semi-solid medium containing methylcellulose and HAT. Five to seven days later, visible clones were removed for subculture in DMEM liquid medium containing 15% FCS and then screened by ELISA. As a result, 12 hybrid cell lines secreting McAbs to the LH-B subunit were obtained. These Abs have been used in clinical diagnostic and immunohistochemical work, showing good results. The advantages of the semi-solid medium in preparing McAbs are discussed.

Animals↗

PubMed1979

Methylcellulose culture of human granulocytic progenitor cells (CFC *): results of bone marrow and blood cultures for normal subjects.

Normal values for the colony-forming ability of granulocytic progenitor cells (CFC) were established for 26 samples of normal bone marrow and 8 samples of normal blood cultured in methylcellulose. The concentration of CFC in the marrow was measured classically as the number of CFC per 10(5) nucleated marrow cells plated and, in addition, as the number of CFC per 10(5) precursors (myeloblasts, promyelocytes, and myelocytes) and per 10(5) metamyelocytes. A value approximating the total number of marrow CFC was calculated by using the number of CFC per 10(5) metamyelocytes. The normal value for circulating CFC were determined as number per ml of blood, per kg of body weight, per 10(5) circulating lymphocytes, and per 10(5) circulating granulocytes. The value of these different modes of expression are discussed. Although the values reported are not directly applicable from one laboratory to the next, they may constitute a useful reference point. Furthermore, they show that the statistical variation in the results is sufficiently small to permit the comparison of values found for pathologic samples with those determined for normal samples.

Adult↗

PubMed1976

Human marrow erythropoiesis in culture. I. Characterization of methylcellulose colony assay.

We examined the morphological and functional characteristics of human marrow erythrocytes cultured with a recently developed methylcellulose colony assay technique. Erythrocytic cells in various stages of development were observed, and a significant degree of maturational synchrony within individual colonies was noted. By light microscopy, colonies consisting of late normoblasts appeared compact, had an orange hue attributable to their hemoglobin, and demonstrated pseudoperoxidase activity, whereas colonies composed of early erythroblasts grew less compact or in clusters of smaller cell aggregates and showed no reddish tinge. Colonies possessing intermediate features were also observed. Maturational synchrony of individual colonies was confirmed using ransmission and scanning electron microscopy. The ultrastructure and cytochemistry of most immature cells were normal. The mature erythrocytes, however, were severely microcytic and hypochromic and contained one to several Heinz bodies. These defects in the cytoplasmic maturation of erythrocytes corresponded with impaired granulocytic maturation in culture, which we observed previously, and suggest environmental or nutritional defects in culture. Linearity of the method was confirmed using five normal bone marrows. Erythropoietin dose-responses observed in ten normal marrows were comparable to the previously reported results and revealed significant variation in individual plating efficiencies.

Adult↗

PubMed2026

Cationic porphyrin covalent organic framework reinforced hydroxypropyl methylcellulose films for photodynamic-photothermal sterilization and food preservation.

Microbial contamination in food necessitates effective antimicrobial packaging. While cellulose-based packaging materials suffer from limited antimicrobial efficacy, lack of active functionality, and susceptibility to inducing microbial resistance. To address these challenges, this study synthesized a cationic porphyrin-based covalent organic framework (Por-ICOF) as a multimodal photosensitizer. Por-ICOF was uniformly dispersed via non-covalent interaction within hydroxypropyl methylcellulose (HPMC), creating an HPMC/Por-ICOF composite film. This integration enhanced mechanical strength (increased by 26%), hydrophobicity (WCA 71°), and gas barrier properties (OP reduced by 42%, WVP reduced by 36%). Under visible light, the HPMC/Por ICOF film superior absorption generated reactive oxygen species (ROS) and photothermal effects, inactivating 99.2% of Escherichia coli and 99.95% of Staphylococcus aureus within 20 min. The composite film exhibited excellent biocompatibility and effectively extended the shelf life of strawberries. This cationic modification strategy for cellulose-based films offers a novel avenue for the design of high-performance antimicrobial food packaging materials.

Food Preservation↗

PubMed1976