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At least 19 recordsLinked to original sources
PubMed1979

Intergeneric and intrageneric inhibition between strains of Propionibacterium acnes and micrococcaceae, particularly Staphylococcus epidermidis, isolated from normal skin and acne lesions.

Two hundred and forty-one strains or resident skin bacteria comprising 93 isolates of Propionob acterium acnes and 148 of Micrococcaceae derived from 36 acne patients and 8 control subjects were screened for their ability to inhibit 32 indicator strains, including 20 strains of P. acnes and 12 strains of Staphylococcus epidermidis derived from patients with all grades of acne and from normal skin. Fifty-three strains (22%) showed some activity against at least one indicator strain. Both broad- and narrow-spectrum inhibition was detected. Inhibitory isolates of P. acnes outnumbered inhibitory Micrococcaceae by four to one. There was a low frequency of inhibition of S. epidermidis by Micrococcaceae (2.7%) and by P. acnes (1.1%) and a higher frequency of inhibition of P. acnes by Micrococcaceae (9.5%) and by P. acnes (40.8%). Furthermore, 81.8% of the subjects sampled possessed strains inhibitory to P. acnes. The significance of this finding is, as yet, unknown. No difference in the prevalence of active strains in normal (20%) and acne (22.5%) skin was detected. These findings suggest that the possession of inhibitory strains and conversely the possession of sensitive strains does not predispose to acne.

Acne Vulgaris↗
PubMed1979

Micrococcaceae isolated from meat and dairy products (taxonomic study).

149 Micrococcaceae strains (35 reference strains and 114 strains isolated from meat and dairy products) have been studied using 61 biochemical microtests. Numerical taxonomy has distinguished two main biochemical groups which may be characterized by their varying ability to use heteroside compounds. -- A small number of biochemical tests in each group enable the differentiation of 17 taxa corresponding to diverse origins. The wild strains of Micrococcaceae found in foods are very different from reference strains in collections. -- With regard to the present study, it seems that a central biotype exists which may be represented by the taxa 13, 14, 15 and 16 in Fig. 1. -- Given this central biotype, various biochemical differences may be considered as ecological adaptability. Group I corresponds to meat orgin -- Taxa No. 1, 2, correspond to collection strains -- Taxon No. 12 corresponds to cheese origin -- Taxa No. 8, 9 and 10 correspond to milk origin.

Bacteriological Techniques↗
PubMed1978

Characterization of Micrococcaceae isolated from post mortem examined pigs.

The investigation comprised 170 cases of infections due to Micrococcaceae among post mortem examined pigs that died during the preweaning period. The incidence was 3.1 promille of the live-born pigs. The infection was most frequent among pigs less than 4 weeks of age (Table I). ACute and subacute generalized infections occurred in 43.5% of the cases (Table II), and exudative epidermitis was the cause of death in 23.5%. In the remaining 32.9% of the cases, the post mortem findings included a variety of debilitating conditions, such as undersize, malformation, starvation or traumata. In all 248 strains of Micrococcaceae were isolated. A preliminary differentiation revealed 41.5% of the strains as Staphylococcus aureus and 34.5% as S. epidermidis and 22.6% as Micrococcus (Table III). A further characterization on 43 of the strains was performed (Table IV and V). Totally 19 different fermentation pattern were observed. In Table IV the strains are grouped into six groups. Fourteen strains were identified as S. aureus and 15 strains as S. epidermidis biotype 2, s. S. hyicus (Table VI). The distribution of the non-haemolytic strains according to biotypes is shown (Table VII). By this grouping, the 15 S. hyicus strains were designated type III B. It is concluded that various staphylococci may be isolated in pure culture from different pathological lesions in preweaned pigs. Biochemical characterization of the strains showed that part of the strains could not be classified by the previously described grouping systems.

Age Factors↗
PubMed1977

Acne vulgaris: an investigation into the number of anaerobic diphtheroids and members of the Micrococcaceae in normal and acne skin.

A quantitative study was made of the microflora of 174 acne and 68 non-acne subjects. Two groups of organisms were investigated, the anaerobic diphtheroids and members of the Micrococcaceae. The results showed high numbers of both groups of bacteria in skin bearing blackheads, papules or pustules and in non-acne adolescent skin. There were significantly lower numbers of bacteria in the pilosebaceous ducts of normal looking skin in acne areas and in pre-adolescent skin when compared with non-acne adolescent skin. It is suggested that increased numbers of bacteria alone do not predispose to acne, but that their interaction with the skin, which is a function of the localized skin environment, may be important.

Acne Vulgaris↗
PubMed1975

Characterization of Micrococcaceae from the urinary tract.

Two hundred and seven urinary strains of staphylococci and micrococci were classified biochemically according to Baird-Parker (1963) and by means of a simplified schema. One hundred and thirteen strains belonged to Staphylococcus aureus, S. epidermids or S. saprophyticus (64 strains) according to the simplified schema, respectively to Baird-Parker's sub-groups SI, SII or M3. S. saprophyticus was isolated from young, female out-patients, was relatively resistant to novobiocin and contained poly AbetaC (beta-N-acetylglucosaminyl ribitol teichoic acid and beta-N-acetylglucosaminyl glycerol teichoic acid). S. aureus and S. epidermidis were isolated from older, male in-patients, were sensitive to novobiocin and contained poly A (N-acetylglucosaminyl ribitol teichoic acid), respectively poly B (glucosyl glycerol teichoic acid). Ninety-four strains belonging to other Staphylococcus or Micrococcus subgroups could not be classified by the simplified schema. With few exceptions, these strains were sensitive to novobiocin and either gave a precipitin reaction corresponding to poly C or were non-typable with the teichoic acid reference systems used. The simplified schema is recommended for the classification of coagulase-negative strains of Micrococcaceae.

Acetylglucosamine↗
PubMed1975

A replica-plating method for the identification of Micrococcaceae.

A procedure of replica plating is described whereby all isolated colonies of Micrococcaceae can be identified with relative ease and rapidity. The method is as accurate as the recommended procedure, but permits a more complete and economical analysis of cutaneous flora in large-scale surveys. In this system, Baird-Parker carbohydrate medium was found somewhat superior to standard medium as was incubation at 35 degrees C instead of the customary 30 degrees C. Baird-Parker's broth medium for acetoin production yielded more positive results than did commercial medium, although the reactions were less distinct. However, an agar acetoin test medium was found as good or perhaps even better than Baird-Parker's medium. The classification schemes of Baird-Parker and Bergey's Manual were contrasted in the analysis of data.

Bacteriological Techniques↗
PubMedDate not supplied

[Value of different diagnostic tests for Micrococcaceae and their implication in pathology].

A number of 1431 products were examined and 200 coagulase-negative micrococcaceae strains were isolated and identified. On the basis of morphological aspects, enzymatic reactions and behavioural testing to antibiotics 18 strains (9%) were listed as Micrococcus and 182 (91%) as Staphylococcus strains. Efficient among the tests used for differentiating between the two genera were : microscopic aspects, pigmentogenesis, resistance to ClNa, anaerobic glucose fermentation, glycerol fermentation in the presence of erythromycin, reduction of nitrates to nitrites, and testing to novobiocin. Other tests, due to their variability, could only be used conjointly, and, finally, a further category had no diagnostic value, such as oxidase and catalase tests or the rhamnose fermentation reaction. Both the Micrococcus and the Staphylococcus strains were isolated from pathological cases and from healthy carriers, and can therefore be listed in the group of potential pathogens.

Bacterial Infections↗
PubMed1978

[Action of egg lysozyme on representatives of the family Micrococcaceae. Its action on Micrococcus].

The results of the study of the effect of various concentrations of egg lysozyme on M. luteus and M. varians using 2 methods, i.e. serial dilutions in agar and turbidimetric are presented. It was found that the MIC of lysozyme for M. luteus ranged within wide limits, from less than 0.0003 to 1 mg/ml. M. varians was stable to lysozyme. The MIC for all the strains was 8 mg/ml. The turbidimetric method provided determination of general regularities in changes of the optical density in all the strains of M. luteus under the effect of various concentrations of lysozyme. On the basis of these data it was possible to consider the method as the most deep means for determining the intraspecies similarities in the surface structures of Micrococcus as compared to the method of serial dilutions in agar. The dynamics of the changes in the optical density of the M. luteus suspension markedly differed from that of M. varians.

Animals↗
PubMed1979

[Action of egg lysozyme on representatives of the family Micrococcaceae. The action on staphylococci].

The results of the study on the effect of various concentrations of lysozyme on staphylococci of three species, i.e. Staphylococcus aureus, Staphylococcus epidermidis and Staphylococcus saprophyticus are presented. The study was performed with the use of the turbidimetric method. It was found that all the staphylococci had a common tendency for changing the optic density (OD) of the suspensions under the effect of lysozyme which was different from that in Micrococcus luteus. However, the dynamics of decreasing the OD in Staph. saprophyticus was characterized by some properties which provided a supposition that the structures of the cell walls in Staph. aureus and Staph. epidermidis were close, while the cell walls in Staph. saprophyticus had some unknown characteristics.

Dose-Response Relationship, Drug↗