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Effects of citrus molasses distillers solubles and molasses on rumen parameters and lactation.

Effects of dietary citrus molasses distillers solubles (0, 6, 12, and 18% of DM) on diet digestibility and ruminal parameters were studied with four fistulated Holstein cows fed each diet in Latin square sequence. Diets were 25% cottonseed hulls with solubles substituted for corn. Dry matter and organic matter digestibilities were increased curvilinearly with peak at 6%. Crude protein digestibility was depressed linearly and ADF curvilinearly with added solubles. Ruminal acetic acid proportion increased linearly and propionic acid decreased curvilinearly. Change in butyric acid was small with 0, 6, and 12% solubles but it increased dramatically with 18%. In a 36-cow (107 cow-period) lactation experiment with corn silage-based and cottonseed hull-based diets (0, 3, 6, and 9% solubles) milk yield increased; the diet containing 6% solubles gave highest production. We compared cane molasses (3 and 6%), liquid hemicellulose extract (3%, Masonex), and liquid lignin sulfonate (3%, Flambeau) added to cottonseed hull diets. Linear increases in milk yield and FCM were obtained with cane molasses; Flambeau and Masonex effects on production were not different from control. With diets and amounts used in these experiments, citrus molasses solubles and cane molasses each at up to 6% of DM nutritionally were superior to corn.

Animal Feed↗

Florida citrus molasses as a fermentation substrate: I. Free amino acids in molasses from early and midseason varieties of citrus fruits during 2 years of production.

Samples of midseason Florida citrus molasses from 10 different manufacturing plants were studied during 2 years of production for suitability as fermentation substrates. They were analyzed for degrees Brix, pH, percentage of protein, and free amino acid content. The following ranges of values were obtained: degrees Brix, 65.4 to 73.0; pH, 4.2 to 6.4; and protein, 3.3 to 5.1%. Although the general characteristics of all samples were quite similar, the total free amino acid content and the number of amino acids differed from year to year and from plant to plant. The free amino acids consistently present were asparagine, aspartic acid, alanine, gamma-amino-butyric, glycine, glutamic acid, proline, serine, and threonine. Occasionally found were arginine, histidine, isoleucine, leucine, lysine, phenylalanine, tyrosine, and valine. An extremely high level of proline was detected in all samples.

Journal Article↗

Value of feather meal in a molasses-based liquid supplement fed to yearling cattle consuming a forage diet.

Hydrolyzed feather meal (FM) was compared to other sources of CP to determine its value as a protein source in a molasses-based liquid supplement. Ruminal N escape values (measured in situ) for FM, ring-dried blood meal, cottonseed meal, and soybean meal approximated reported values. Ruminal N escape value for catfish meal was lower than values reported for other fish meals. In two trials (194 d), 84 yearling heifers were allotted to 12 pastures (3 pastures/treatment) and fed one of four supplements (1.7 kg of DM.heifer-1.d-1) containing either molasses and urea; molasses, urea,and FM; molasses, urea, FM, and catfish meal; or molasses, urea, FM, and catfish oil. Heifers were exposed to bulls for 60 d. In two trials, 112 steers were allotted to 16 pastures (4 Pastures/treatment) and fed one of four supplements (1.7 kg of DM.steer-1.d-1) containing molasses and urea; molasses, urea and FM; molasses, urea, FM and ring-dried poultry blood meal; or molasses, urea, and poultry feathers and blood hydrolyzed together. In all trials, cattle fed molasses containing FM had faster gains (P < .05) and heifers had heavier live weights at breeding (P < .05) and higher pregnancy rates (P < .05) than cattle fed molasses and urea. Combining poultry blood with molasses, urea, and FM improved (P < .05) steer gains in one of two trials. Combining catfish meal with molasses, urea, and FM did not improve (P > .05) ADG of pregnancy rate of heifers. Combining catfish oil with molasses, urea, and FM increased (P < .05) ADG in one trial and blood cholesterol and live weight of heifers at breeding in both trials. It is concluded that protein feeds, such as FM, that contain a large portion of ruminally undegradable protein may provide much of the CP in liquid supplements fed to growing cattle consuming moderate-quality forage. Adding catfish oil to a liquid supplement may improve ADG and live weight of yearling heifers at breeding.

Aging↗

Effect of forage quality on digestion and performance responses of cattle to supplementation with cooked molasses blocks.

We evaluated the effect of forage quality on response of cattle to supplementation with cooked molasses blocks. In Exp. 1, 175 heifers had ad libitum access to prairie hay (5.2% CP, DM basis). Treatments were a 2 x 3 factorial: supplementation with 0 or 1.96 kg/d of alfalfa DM, and supplementation with no cooked molasses block or with a low-protein or a high-protein cooked molasses block (14.4 and 27.5% CP, respectively, DM basis). There were no significant interactions between alfalfa and cooked molasses block for intake or gain. Forage intake and ADG were increased (P < 0.05) by alfalfa supplementation. Heifers fed high-protein cooked molasses blocks gained more (P < 0.05) weight than those fed low-protein cooked molasses blocks or no cooked molasses block. Heifers fed high-protein cooked molasses blocks ate more (P < 0.05) forage than those fed low-protein cooked molasses blocks, with heifers fed no cooked molasses block being intermediate. In Exp. 2, responses to cooked molasses blocks containing 33% CP (DM basis) were measured in 18 steers fed: 1) brome (8.4% CP), 2) alfalfa (19.2% CP), or 3) brome supplemented with 1.93 kg/d of alfalfa DM. Forages were available ad libitum. Forage DM intake was not affected by cooked molasses block and was greater (P < 0.05) for alfalfa than the alfalfa/brome mix, which in turn was greater (P < 0.05) than brome. Digestibility of DM was greater (P < 0.05) for alfalfa than brome or the alfalfa/brome mix and was not affected by cooked molasses block supplementation. Supplementation with cooked molasses blocks had only small effects on intake and digestion of medium- to high-quality forages, but it improved gains and feed efficiencies of heifers fed prairie hay ad libitum, with or without supplemental alfalfa.

Animal Feed↗

Dietary interaction of cane molasses with source of roughage: intake and lactation effects.

Effects of cane molasses at 0, 4, and 8% of DM in complete mixed diets were evaluated when molasses was fed to lactating dairy cows with cottonseed hulls, alfalfa haylage, or both combined. Thirty-six mature Holstein cows from mid to late lactation were used in a partially balanced incomplete block design with three 28-d periods. Variables measured were DM intake, milk yield, and composition. With cottonseed hull diets (30% of DM), molasses improved milk yield, milk fat percentage, solids-corrected milk, and feed efficiency. Dry matter intake was not affected by molasses. With alfalfa haylage diets (35% of DM), 8% molasses depressed actual milk yield and solids-corrected milk, DM intake, milk fat percentage, milk protein percentage, and feed efficiency. Milk fat percentage was increased with 4% molasses. With 65% alfalfa haylage diets, most variables measured were unaffected by molasses; however, with 8% molasses, DM intake increased and milk protein percentage decreased. In diets with both roughages combined, molasses did not affect any variable measured; however, these diets gave highest solids-corrected milk yields. Results show clearly that molasses effects depend on percentage molasses and type and amount of roughage in the diet.

Animal Feed↗

Effects of molasses grass, Melinis minutiflora volatiles on the foraging behavior of the cereal stemborer parasitoid, Cotesia sesamiae.

Olfactory responses of the cereal stemborer parasitoid Cotesia sesamiae to volatiles emitted by gramineous host and nonhost plants of the stemborers were studied in a Y-tube olfactometer. The host plants were maize (Zea mays) and sorghum (Sorghum bicolor), while the nonhost plant was molasses grass (Melinis minutiflora). In single-choice tests, females of C. sesamiae chose volatiles from infested and uninfested host plants and molasses grass over volatiles from the control (soil). In dual-choice tests, the wasp preferred volatiles from infested host plants to those from uninfested host plants. There was no discrimination between molasses grass volatiles and those of uninfested maize, uninfested sorghum, or infested maize. The wasp preferred sorghum volatiles over maize. Combining uninfested maize or sorghum with molasses grass did not make volatiles from the combination more attractive as compared to only uninfested host plants. Infested maize alone was as attractive as when combined with molasses grass. Infested sorghum was preferred over its combination with molasses grass. Local growth conditions of the molasses grasses influenced attractiveness to the parasitoids. Volatiles from Thika molasses grass were attractive, while those from Mbita molasses grass were not. Growing the Thika molasses grass in Mbita rendered it unattractive and vice versa with the Mbita molasses grass. This is a case of the same genotype expressing different phenotypes due to environmental factors.

Animals↗

Molasses increases HDL cholesterol in rats research note.

Two experiments were conducted to determine whether molasses might exert effects on serum lipoproteins. In experiment 1, 24 rats were divided into two groups and fed diets containing liquid molasses from sugar beet or sucrose (7.71 g of molasses dry matter or sucrose per kg of diet). The second experiment included four groups of rats (n = l2/group) and was conducted in a bifactorial design, with the factors being molasses (non-supplementation vs. supplementation of 77.1 g of molasses dry matter per kg of diet at the expense of sucrose) and dietary cholesterol (0 vs. 5 g/kg diet). In experiment 1, the ratio of low-density lipoprotein (LDL) to high-density lipoprotein (HDL) cholesterol concentration tended to be lower in rats fed the molasses diet than in rats fed the control diet (p < 0.15). In experiment 2, rats fed the molasses diet had higher concentrations of HDL cholesterol (+ 26%) than control rats fed diets without molasses (p < 0.05). This effect was independent of the dietary cholesterol concentration. Concentrations of cholesterol in LDL, very low-density lipoprotein (VLDL), and liver as well as concentrations of triacylglycerols in plasma and liver remained unaffected by molasses in both experiments. In conclusion, the results of this study suggest that supplementation of molasses is effective at raising HDL cholesterol levels in rats.

Animals↗

Effect of lasalocid sodium and molasses on performance of fattening lambs and on rumen liquor and blood parameters.

Two experiments were conducted to evaluate the effects of sugar beet molasses (0, 8 and 16%) with or without lasalocid sodium (33 ppm) on rumen liquor and blood parameters of sheep, and on fattening reared Karagouniko breed lambs. In Expt. 1 six adult Karagouniko fistulated wethers were fed six different pelleted diets. The results showed that lasalocid decreased (P less than 0.01) total VFAs concentration in the rumen (7.1%) by reducing (P less than 0.001) acetic, n-butyric and iso-valeric acids while increased (P less than 0.001) propionic acid concentrations. The same trends were observed in molar proportions of the individual VFAs. The total VFAs production was also depressed by 13.75% when molasses were used at the level of 16%. No interaction among the main treatments was observed with the exception of L X M with 16% molasses where the propionic acid concentration decreased, and those of butyric and iso-valeric acids increased. Lasalocid did not alter the mean values of rumen liquor pH and ammonia nitrogen concentration while molasses increased the later parameter. No significant differences on dietary protein degradability were observed among treatments. Lasalocid reduced the beta-HBA concentration by 13% in the blood while molasses increased it (P less than 0.001). Free fatty acids' concentration was decreased by the molasses inclusion to the diets, while lasalocid didn't alter blood glucose concentration. Finally, the number of protozoa in the rumen liquor was reduced by 49 and 70% at 0 and 5 hours post feeding respectively with the addition of lasalocid to the diets, regardless of the use of molasses. In Expt. 2 utilized 86 weaned male Karagouniko fattening lambs, with initial mean LW 13 kg, for 60 days. They were assigned to six groups and fed ad libitum with diets of the same composition as in Exp 1. Lasalocid increased the growth rate by 8.6%, reduced feed consumption by 4.8% (P less than 0.005) and improved feed conversion by 11.8%. Molasses also improved growth rate by 16-34% and increased feed consumption by 15-22%. The interaction of L X M to feed consumption was significant (P less than 0.001). None of the treatments had an effect on carcass characteristics. Finally lasalocid showed its positive action when it was used in combination with molasses in ruminants' diets.

3-Hydroxybutyric Acid↗

A cost-effective cane molasses medium for enhanced cell-bound phytase production by Pichia anomala.

AIM: Formulation of an inexpensive cane molasses medium for improved cell-bound phytase production by Pichia anomala. METHODS AND RESULTS: Cell-bound phytase production by Pichia anomala was compared in synthetic glucose-beef extract and cane molasses media. The yeast was cultivated in 250 ml flasks containing 50 ml of the medium, inoculated with a 12 h-old inoculum (3 x 10(6) CFU ml(-1)) and incubated at 25 degrees C for 24 h at 250 rev min(-1). Different cultural parameters were optimized in cane molasses medium in batch fermentation. The cell-bound phytase content increased significantly in cane molasses medium (176 U g(-1) dry biomass) when compared with the synthetic medium (100 U g(-1) dry biomass). In fed-batch fermentation, a marked increase in biomass (20 g l(-1)) and the phytase yield (3000 U l(-1)) were recorded in cane molasses medium. The cost of production in cane molasses medium was pound 0.006 per 1000 U, which is much lower when compared with that in synthetic medium (pound 0.25 per 1000 U). CONCLUSIONS: An overall 86.6% enhancement in phytase yield was attained in optimized cane molasses medium using fed-batch fermentation when compared with that in synthetic medium. Furthermore, the production in cane molasses medium is cost-effective. SIGNIFICANCE AND IMPACT OF THE STUDY: Phytase yield was improved in cane molasses when compared with the synthetic medium, and the cost of production was also significantly reduced. This enzyme can find application in the animal feed industry for improving the nutritional status of feed and combating environmental pollution.

6-Phytase↗

Effect of molasses-based liquid supplements on digestibility of creeping bluestem and performance of mature cows on winter range.

Poor quality of tropical range forage leads to excessive weight loss and poor reproductive performance of beef cows. This research measured 1) the digestibility by steers fed creeping bluestem (Schizachyrium scoparium var. stoloniferum) diets supplemented with cane molasses (0 or 20% of diet DM) and(or) added CP (no added CP, urea, or soybean meal) and 2) the performance of mature cows grazing creeping bluestem range and supplemented with 1.6 kg/(cow.d) (DM basis) of either molasses-urea or molasses-cottonseed meal-urea, each containing 30% CP (DM basis). Bluestem averaged 4.7% CP and 37.0% in vitro OM digestion. In the digestion trial, diets supplemented with molasses had greater (P < .01) apparent OM digestibility but lower (P < .01) apparent NDF, ADF, and hemicellulose digestibilities than diets not supplemented with molasses. Apparent OM digestibility of diets supplemented with soybean meal was greater (P < .05) than that of diets supplemented with urea but not different (P > .05) from the control. While grazing range, mature cows supplemented with molasses-urea lost less (P < .05) body condition (-1.7 vs -1.3) than cows supplemented with molasses-cottonseed meal-urea. Treatment did not affect cow weight loss on range (P = .85) or pregnancy rate (P = .51). Cows supplemented with molasses-urea tended to wean a heavier calf (P = .09). When fed at 1.6 kg/(cow.d) to mature cows grazing range, a molasses-based supplement containing urea was of equal value to one containing a natural protein.

Animal Feed↗

Identification of genes whose expressions are enhanced or reduced in baker's yeast during fed-batch culture process using molasses medium by DNA microarray analysis.

Genes whose expression levels are enhanced or reduced during the cultivation process that uses cane molasses in baker's yeast production were identified in this study. The results showed that baker's yeast grown in molasses medium had higher fermentation ability and stress tolerance compared with baker's yeast grown in synthetic medium. Molasses apparently provided not only sugar as a carbon source but also provided functional components that enhanced or reduced expression of genes involved in fermentation ability and stress tolerance. To identify the genes whose expression is enhanced or reduced during cultivation in molasses medium, DNA microarray analysis was then used to compare the gene expression profile of cells grown in molasses with that of cells grown in synthetic medium. To simulate the commercial baker's yeast production process, cells were cultivated using a fed-batch culture system. In molasses medium, genes involved in the synthesis or uptake of vitamins (e.g., biotin, pyridoxine and thiamine) showed enhanced expression, suggesting that vitamin concentrations in molasses medium were lower than those in synthetic medium. Genes involved in formate dehydrogenase and maltose assimilation showed enhanced expression in molasses medium. In contrast, genes involved in iron utilization (e.g., siderophore, iron transporter and ferroxidase) showed enhanced expression in synthetic medium, suggesting that iron starvation occurred. The genes involved in the metabolism of amino acids also showed enhanced expression in synthetic medium. This identification of genes provides information that will help improve the baker's yeast production process.

Culture Media↗

Effects of base ingredient in cooked molasses blocks on intake and digestion of prairie hay by beef steers.

Twelve steers (332 kg) were used in three simultaneous 4 x 3 incomplete Latin squares to evaluate effects of beet molasses (BEET), cane molasses (CANE), or concentrated separator by-product (CSB) as base ingredients in cooked molasses blocks on intake and digestion of prairie hay and ruminal characteristics. All steers had ad libitum access to prairie hay (5.9% CP and 69.4% NDF; DM basis). The four experimental treatments included a control (no supplement) and three cooked molasses blocks, based on BEET, CANE, or CSB, fed daily at .125% of BW (.42 kg/d as-fed, .13 kg/d CP). Forage OM, NDF, and N intakes; digestible OM, NDF, and N intakes; and total tract OM and N digestibilities (percentage of intake) were greater (P < .05) for steers fed cooked molasses blocks than for control steers. Total tract OM digestibility was greater (P < or = .06) for steers fed BEET blocks (54.0%) than for those fed CSB (52.1%) or CANE blocks (52.2%). Digestion of NDF was greatest (P < .05) for steers fed BEET blocks (51.9%) and tended to be greater (P < .07) for steers fed CANE (49.3%) or CSB blocks (49.3%) than for control steers (46.9%). Ruminal ammonia concentrations were greater (P < .05) for steers fed cooked molasses blocks (.89 mM) than for control steers (.21 mM); this was primarily due to increases to 4.6 mM at 2 h postfeeding for steers fed blocks. Concentrations of total VFA in ruminal fluid were greater (P < .05) for steers fed BEET (92.7 mM) and CSB (88.1 mM) blocks than for control steers (80.3 mM), whereas concentrations for steers fed CANE blocks were intermediate (85.4 mM). Steers supplemented with cooked molasses blocks had greater molar percentages of butyrate than did control steers, particularly shortly after feeding. In summary, supplementation with cooked molasses blocks increased forage intake and digestion. The three base ingredients elicited similar responses, although steers fed BEET had slightly greater OM and NDF digestibilities than those fed CANE or CSB.

Animal Feed↗

Cultivation of the carotenoid-hyperproducing mutant 2A2N of the red yeast Xanthophyllomyces dendrorhous (Phaffia rhodozyma) with molasses.

The carotenoid-hyperproducing mutant 2A2N of the yeast Xanthophyllomyces dendrorhous (Phaffia rhodozyma) was cultivated using sugar beet blackstrap molasses. This molasses was composed of 70% (w/v) total solid and 50% (w/v) total sugar. Biomass yield (biomass/carbohydrate) significantly decreased at >5% (v/v) molasses. Atomic emission spectrometry revealed that Na and P were the limiting nutrients when molasses was used. Molasses (5%, v/v) containing urea (30 g/l molasses) and sodium phosphate (NaH2PO4, 5 g/l molasses) was formulated for biomass production by the mutant. The optimal pH for carotenoid production was 4.9 during the growth phase and 2.6-3.5 during the stationary phase. The three main sugars in molasses (sucrose, glucose, and fructose) were assimilated by the mutant but fructose was consumed slowly. When the formulated medium with pH 4.5-5.5 was used, the maximal biomass yield was 36 g/l (0.18 g of yeast l(-1)h(-1) and 40 mg of carotenoid l(-1)) in fed-batch pilot-scale 100-l cultivation.

Journal Article↗

Decolorization of molasses and a dye by a newly isolated strain of the fungus Geotrichum candidum Dec 1.

A fungus, Geotrichum candidum Dec 1, newly isolated as a dye-decolorizing microorganism, was used to decolorize molasses and an anthraquinone dye in shaken flasks. A degree of decolorization of molasses of 87% was achieved after 12 days of cultivation, and the maximum rate of decolorization of the dye in the culture broth was obtained in 7 days. The apparent activity of peroxidase in the molasses, which is responsible for dye decolorization, was significantly lower than that of purified peroxidase, due to the inhibition by molasses, but the inhibition was reduced after the fungus was fully grown. As two ultrafiltered fractions of molasses were similarly decolorized by Dec 1, Dec 1 apparently degraded colored substances of a wide range of molecular weights. When Dec 1 was cultivated in a medium in which sucrose in the molasses was hydrolyzed with invertase, the degree of decolorization of molasses, and rate of decolorization of the dye were similar to these obtained above.

Biodegradation, Environmental↗