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A comparison of sampling methods with respect to cattle-visiting Muscidae and their nematode infections.

Cattle-visiting Muscidae were sampled with a handnet from a blood-smeared Masonite panel as well as grazing heifers and dairy cows in south-central Sweden. All flies were examined in search of nematodes. Only Musca autumnalis was infected with nematodes (Parafilaria bovicola, Thelazia spp. and Heterotylenchus autumnalis). The only mixed infections found involved P. bovicola and Thelazia spp. Most M. autumnalis infected with P. bovicola and Thelazia spp. were found during the two first months of the grazing season, whereas the prevalence of specimens infected with H. autumnalis remained about the same throughout the sampling period. Musca autumnalis was caught in significantly smaller numbers from heifers (P < 0.05) than from panel and cows. The catches of nematode-infected M. autumnalis were similarly distributed. Catches of other cattle-visiting Muscidae did not differ significantly among the methods used. Handnet sampling off a panel is therefore considered to be a convenient and accurate method when investigating the abundance of cattle-visiting Muscidae and the prevalence of their nematode infections.

Animals

Description and biology of Trichopria painteri n.sp. (Hymenoptera: Diapriidae), a solitary parasitoid of Stomoxys calcitrans (Diptera: Muscidae) from Harare, Zimbabwe.

Taxonomic description and life history are given of Trichopria painteri n.sp. (Hymenoptera: Diapriidae), a solitary endoparasitoid that emerged from Stomoxys calcitrans (L.) (Diptera: Muscidae) pupae collected at an agricultural installation near Harare, Zimbabwe, Africa. Although its low level of parasitism. high immature mortality and short adult life span would require augmentative releases of T.painteri, this parasitoid could reduce isolated field populations of S.calcitrans to an acceptable level within several weeks.

Animals

[Seasonal dynamics of synbovine flies (Diptera: Muscidae) during the grazing period in Jarov].

Synbovine flies and the seasonal pattern of their occurrence were studied throughout the grazing period in the cattle pastures of the Strakonice district. Skufin's traps were used for catching the flies. The total number of dipterous insects caught during the series of trappings in two- to three-week intervals was 2096. The family Muscidae (23 species) was represented most amply in the sample catches (Musca autumnalis 65.9%, Hydrotaea Meteorica 20.4%, Hydrotaea irritans 4.2%, Haematobosca stimulans 2.6%). The maximum occurrence of haematophagous species was recorded at the beginning and at the end of the grazing season. The maximum occurrence of secretophagous species was recorded in June.

Animals

Aspects of the reproductive biology of some cattle-visiting Muscidae (Diptera) in north-east England.

During studies of flies that visit farm animals in north-east England, samples of Muscidae were obtained on a regular basis from grazing animals (mainly cattle) and from Manitoba traps. Female flies were examined to determine their wing length, degree of wing damage, whether or not they had mated, whether their crop or gut contained blood, the stage they had reached in ovarian development and the number of eggs present. This information was used to assess the timing of mating, the stage at which blood meals were taken and the potential fecundity of the more numerous species, especially Hydrotaea irritans (Fallén) and Morellia simplex (Loew). A relationship between the size of the individual and the number of eggs it carried was found in several species.

Animals

The genome sequence of the muscid fly, Hydrotaea similis Meade, 1887 (Diptera: Muscidae).

We present a genome assembly from an individual female Hydrotaea similis (muscid fly; Arthropoda; Insecta; Diptera; Muscidae). The assembly contains two haplotypes with total lengths of 884.66 megabases and 852.78 megabases. Most of haplotype 1 (90.83%) is scaffolded into 5 chromosomal pseudomolecules. Haplotype 2 was assembled to scaffold level. The mitochondrial genome has also been assembled, with a length of 20.27 kilobases. This assembly was generated as part of the Darwin Tree of Life project, which produces genomes for eukaryotic species found in Britain and Ireland.

Diptera

The genome sequence of a muscid fly, Lispocephala verna (Fabricius, 1794) (Diptera: Muscidae).

We present a genome assembly from an individual female Lispocephala verna (muscid fly; Arthropoda; Insecta; Diptera; Muscidae). The assembly contains two haplotypes with total lengths of 987.71 megabases and 934.62 megabases. Most of haplotype 1 (95.8%) is scaffolded into 5 chromosomal pseudomolecules. Haplotype 2 was assembled to scaffold level. The mitochondrial genome has also been assembled, with a length of 16.34 kilobases. This assembly was generated as part of the Darwin Tree of Life project, which produces genomes for eukaryotic species found in Britain and Ireland.

Diptera

The genome sequence of a muscid fly, Hydrotaea militaris (Meigen, 1826) (Diptera: Muscidae).

We present a genome assembly from an individual female Hydrotaea militaris (Arthropoda; Insecta; Diptera; Muscidae). The assembly contains two haplotypes with total lengths of 1 703.03 megabases and 1 760.39 megabases. Most of haplotype 1 (96.2%) is scaffolded into 6 chromosomal pseudomolecules. Haplotype 2 was assembled to scaffold level. The mitochondrial genome has also been assembled, with a length of 18.83 kilobases. This assembly was generated as part of the Darwin Tree of Life project, which produces genomes for eukaryotic species found in Britain and Ireland.

Diptera

Synanthropy of Anthomyiidae, Muscidae and Calliphoridae (Diptera) in Cuba.

The material of 22302 flies of Anthomyiidae, Muscidae and Calliphoridae collected by trap-method in Cuba 1966 was qualitatively and quantitatively analysed. Relative density, seasonal incidence and food preference were ascertained in 20 species. In potential vectors of human diseases the ecological characters and classification of synanthropy are given.

Animal Feed

Studies on flies in Korea. III. Taxonomic studies on muscid flies (Diptera, Muscidae).

Forty species belonging to 14 genera of the family Muscidae were found so far in Korea. They are described in this paper and 5th sternites, forceps and male genitalia are illustrated. Eleven species recorded by Kobayashi (1918-1940) and 16 species by Hori (1952) were revised and redescribed in this paper. The key to the species of the muscid flies in Korea are also presented.

Animals

Studies on medically important flies in Thailand. V. On 32 species belonging to the subfamilies Muscinae and Stomoxyinae including the taxonomic keys (Diptera: Muscidae).

Flies from different geographical parts of Thailand were collected to study their medical importance as well as making a zoogeographical study. After examination, 32 species of 10 genera, Orthellia, Mitroplatia, Morellia, Pyrellia, Rypellia, Haematobia, Haematobosca, Stygeromyia, Haematostoma and Stomoxys, belonging to Muscidae were identified and these are reported in this paper. Some of these are newly recorded ones from this country. This study is based on the specimens available in the authors' collection.

Animals

Note on some diptera (Tabanidae, Glossinidae, Muscidae and related families) at the peak of dry season in Toro Game Reserve, Uganda.

The authors studied the occurrence of haematophagous and non-haematophagous flies in three different collecting sites in the Toro Game Reserve in western Uganda, at the dry season peak (January, February), using canopy trap and baited traps. Collected were 6 species of Tabanidae, 1 species of Glossinidae, 24 species of Muscidae, 3 species of Calliphoridae, 4 species of Sarcophagidae and 3 species of other families.

Animals

Allozyme variation in stable flies (Diptera: Muscidae).

Polyacrylamide gel electrophoresis was used to resolve allozymes in the cosmopolitan blood-feeding stable fly, Stomoxys calcitrans (L.). Nineteen of 38 loci were polymorphic (53%). Mean heterozygosities among all loci and among only polymorphic loci were 0.096 and 0.182, respectively. These gene diversity measures are about half those among other muscid Diptera. Variation in gene frequencies was examined in 10 natural stable fly populations from Iowa and Minnesota. Gene frequencies were homogeneous at five of eight loci among six populations in 1990 and eight of eight loci among four populations in 1992. Wright's F statistics showed no significant departure from random mating among stable flies. It was concluded that gene flow compensates for any local differentiation in stable fly populations.

Animals

Ivermectin cattle pour-on: duration of a single late spring treatment against horn flies, Haematobia irritans (L.) (Diptera: Muscidae) in Missouri, USA.

Late spring treatment of cattle with a single dose of pour-on ivermectin (0.5 mg kg-1 body weight) resulted in reduced horn fly populations for approximately 6 weeks, with percentage efficacy exceeding 80% for at least 26 days post-treatment. Bioassay with house flies of dung from treated cattle demonstrated suppression of fly emergence for 11 days post-treatment. The subsequent increase in horn fly numbers on the treated herd was predicted by a developmental rate equation interpreted by the time of cessation of larval fly inhibition in field dung.

Administration, Topical

Moxidectin: pharmacokinetics and activity against horn flies (Diptera: Muscidae) and trichostrongyle nematode egg production.

The concentration of moxidectin, a macrocyclic lactone endectocide, in the blood serum of cattle resulting from single and daily subcutaneous injections and oral dosing was determined as a function of time. When given as a single subcutaneous (SC) injection, the drug peaked between 4 and 6 h post-treatment. As a single oral dose, the peak serum level occurred at 1 day post-treatment. Daily SC injections and oral doses resulted in a gradual increase in blood serum level over the 21 days of treatment but did not reach a plateau during this time. Horn flies, Haematobia irritans (Linnaeus), feeding on the blood of treated cattle drawn on Day 21 of daily treatment showed a decline in survival and egg production, but a negligible effect on egg hatching. Dose-mortality data on adult horn flies showed an LC-50 and LC-90 value of 10 ppb and 19 ppb in the blood, respectively. Moxidectin was also found to have larvicidal activity against the immature stages of the horn fly in the manure of treated cattle. Moxidectin administered at 100, 50 and 25 micrograms kg-1 as a daily oral medication was 100% effective in eliminating trichostrongyle egg counts by Day 3 of the treatment. Counts remained negative to the end of the trial.

Administration, Oral

Prehibernating Musca autumnalis (Diptera: Muscidae)--an overwintering host for parasitic nematodes.

Diapausing face flies, Musca autumnalis (De Geer), are characterized by the presence of hypertrophied fat bodies (HFB) and arrested ovarian development. A total of 1752 face flies were caught around cattle in south-central Sweden during the last 5 weeks of the grazing season, in August-September. Females in this material (1139) were examined in search of HFB and to determine their reproductive status. It was assumed a priori that gonoactive flies would not undergo diapause. Flies were also dissected in search of the nematodes Parafilaria bovicola (Tubangui), Thelazia spp. and Heterotylenchus autumnalis (Nickle). Only females were found to be infected, of which 0.6% contained P. bovicola, 0.6% Thelazia spp. and 1.4% H. autumnalis. Both gonoactive and nongonoactive flies were infected with P. bovicola and Thelazia spp. Only nongonoactive Thelazia-infected flies had HFB, which is suggestive of flies able to overwinter. In the Heterotylenchus-infected flies with HFBs, arrested development (hypobiosis) of the nematode was recorded.

Animals