PubMed Health⌕ Search

SEARCH · PubMed Health

Results for “Naphthol AS D Esterase”

Explore indexed PubMed citations for clinical trials, systematic reviews and public health research. Read source abstracts and follow each citation to its original PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 19 recordsLinked to original sources
PubMed1977

[Nonspecific esterase and naphthol-AS-D-chloroacetate esterase in monocytoid and myeloid cells of healthy cattle and cattle suffering from leukosis].

The reaction to non-specific esterase can be used for the identification of the monocytoid cells of the periphery. A negative reaction is exhibited by neutrophile and eosinophile leucocytes and erythrocytes. Varying results are obtained from lymphocytes, rendering it impossible to use this method in the group of lymphoid cells of the periphery or marrow. In the group of large marrow cells (promonocytes), non-specific esterase gave a very strong reaction; this applies both to the marrow of healthy cattle and cattle suffering from leucosis. For the time being, efforts to use this reaction for the solution of the problem of the differentiation of monocytoid cells and cells of similar size in the myeloid series of the bone marrow have not been successful. In neutrophile leucocytes of the periphery, naphtol-AS-D-chloroacetate esterase gives a less intensive reaction than in humans. For this reason, it is less suitable for the differentiation of these cells. Other cell types (eosinophile leucocytes, monocytes, lymphocytes, erythrocytes as well as their bone-marrow stages) give a negative reaction. In the group of large marrow cells of the myeloid series (promyelocytes and neutrophile myelocytes), naphtol-AS-D-chloracetate esterase shows a more intensive reaction in healthy cattle, as compared with cattle suffering from leucosis.

Animals↗
PubMed1986

Naphthol-AS-D-chloroacetate esterase activity in globule leucocytes in the tracheal epithelium of rats.

The enzyme naphthol-AS-D-chloroacetate esterase is histochemically demonstrated in the granules of globule leucocytes in the tracheal epithelium of rats. The enzyme reactivity may be used as a cytochemical marker of these cells. The previously postulated mast cell origin of globule leucocytes is doubted, and the possibility that globule leucocytes belong to the group of natural killer cells is discussed. The biological role of the located esterase and the function of the globule leucocytes are also briefly discussed.

Animals↗
PubMed1983

Investigation of granulocytopoietic kinetics by microdensitometric evaluation of primary granule naphthol-AS-D-chloroacetate esterase activity.

Using a scanning microscope photometer we determined quantitatively the enzymecytochemical reaction product for naphthol-AS-D-chloroacetate esterase in neutrophilic granulocytes and their precursors in man. Evaluation of neutrophilic cells from three healthy donors resulted in a logarithm-normal distribution. After subdivision of these cells in their morphologically defined maturational stages no statistically bimodal distribution was shown within the single cell groups. Myelocytes showed twice the amount of the polymorphonuclear neutrophil absorption values. The highest promyelocyte obsorptions were double the values of the myelocyte absorptions. The standard deviation of the absorbance obtained with promyelocytes (which encompass cells already producing granules up to cells reaching their maximal granule content) was significantly higher than the standard deviation of the myelocytes. As already known, primary granules are only synthesized at the promyelocyte stage and - according to the present knowledge - their chloracylesterase and peroxidase activities are not lost during further maturation. Consequently, our results indicate that only enzyme-rich, late promyelocytes undergo mitosis transforming into myelocytes. Correspondingly, their absorption value was halved. Since the absorbance from myelocytes to polymorphonuclears is again halved, myelocytes divide only once. Metamyelocyte absorptions in part correspond to that of myelocytes. This indicates that no distinction can be made between myelocytes with mitotic capacity and "true" if only the size and the nuclear shape are considered metamyelocytes which are not longer capable of undergoing mitosis.

Adult↗
PubMed1981

[Occurrence of naphthol-AS-D-chloracetate-esterase-activity in mast cells with special references to cutaneous mastocytoses (author's transl)].

The enzyme naphthol-AS-D-chloracetate esterase (NASDCE) was demonstrated histochemically in mast cells of tissues of laboratory animals and men under normal and pathological conditions using the method after Leder (1964). Investigating the possible action of different fixations as Bouin's and Carnoy's fluid as formaldehyde also cryostate sections were studied for comparison. After tissue fixation the activity of the NASDCE is localized strongly in the granules of mast cells. The type of fixation is without influence on the histochemical reaction and localization. Differences concerning the investigated species could not be demonstrated. In benign neoplasias of mast cells such as diffuse (urticaria pigmentosa) and localized cutaneous mastocytosis as well as benign systemic mastocytosis with skin involvement (Sagher) the granule bound reaction is very intensive. The NASDCE can be recommended as a marker for the microscopical localization of mast cells, especially in the histological diagnosis of their benign and malignant neoplasias.

Animals↗
PubMed1993

Relationship between naphthol AS-D chloroacetate esterase and prostaglandin synthase.

The close topographical correlation between naphthol AS-D chloroacetate esterase-positive macrophages and prostaglandin synthase-rich macrophages in the thymus of normal and cyclosporin-treated rats was observed. It seems possible that chloroacetate esterase is an enzyme related to metabolism of arachidonic acid and/or production of its active metabolites.

Animals↗
PubMed1985

Naphthol AS D chloroacetate esterase-positive macrophages in the cortico-medullary zone of the normal rat thymus.

Naphthol AS D chloroacetate esterase (NASDCE)-positive macrophages are positioned in the cortico-medullary zone (CMZ) of the normal rat thymus. These cells contain the very strongly NASDCE-positive granules of varying size in the cytoplasm. An identical distribution within the thymic parenchyma and an identical morphological appearance is observed in CMZ macrophages after staining with aldehyde fuchsin. The incubation of thymic sections in 10% formalin at pH 7.0 for 48 h does not inhibit the activity of NASDCE in CMZ macrophages. The activity of nonspecific esterase is almost totally abolished by this treatment: only the single, largest globular inclusion within some of the cells remains weakly or moderately positive. The granular content of the CMZ macrophages does not stain metachromatically with toluidine blue and these cells are endogenous peroxidase-negative. NASDCE-positive thymic macrophages are easily distinguished from: a) NASDCE-positive mast-cells, which are confined to the capsular and septal connective tissue and contain densely packed metachromatic granules, and b) NASDCE-positive neutrophilic granulocytes, which have a specific morphological appearance and show very strong endogeneous peroxidatic activity.

Animals↗
PubMed1982

Freeze-preservation of staining solutions in enzyme histochemistry.

Staining solutions of acid phosphatase and esterase group (naphthol-AS-D-chloroacetate esterase, naphthol-AS-acetate esterase and alpha-naphthyl-acetate esterase) were stored for 6 months using the freezing facilities, and the staining results were compared with those of each set of controls. The results showed that the staining solutions of all enzymes were stable for long-term preservation (at least 6 months) in the deep freezer (-80 degrees C) when they were stored appropriately in separated forms. In such a freezing procedure, the staining results for each enzyme did not show any decrease of positive intensity nor any non-specific staining.

Acid Phosphatase↗
PubMed1991

[Esterase activity of neutrophils and monocytes in patients with stenocardia and myocardial infarction].

Neutrophils and monocytes of coronary patients and healthy subjects were investigated for activity of alpha-naphthylacetate esterase, naphthol--AS--D+chloroacetate esterase, alpha-naphthylbutyrate esterase. This activity varied from healthy to coronary subjects, among anginal and MI sufferers, etc. The fact made the authors to suggest comprehensive evaluation of monocyte and neutrophil esterase activity as a diagnostic and prognostic indicator.

Adult↗
PubMed1979

Chronologic changes of activities of naphthol AS-D acetate esterase and other nonspecific esterases in the mononuclear phagocytes of tuberculous lesions.

Nonspecific esterases of mononuclear phagocytes (MNs) were studied histochemically in the developing and healing tuberculous lesions produced in rabbit skin by bacille Calmette Guérin (BCG). Nonspecific esterases were assayed with the following substrates: naphthol AS-D acetate (AS-D), naphthol AS-D chloroacetate (AS-D Chl), naphthol AS acetate (AS) and alpha-naphthyl acetate (alpha-N), beta-Galactosidase, a lysosomal enzyme of MNs, was also assayed as a marker of MN activation. The number of MNs hydrolyzing AS-D Chl, AS, and alpha-N increased for 2 to 4 weeks after infection. These chronologic changes were similar to that of beta-galactosidase. In contrast, MNs hydrolyzing AS-D appeared predominantly in the healing lesions five to six weeks after infection. These MNs had the morphologic features of balloon-like cells. They contained few lysosomes and gathered in clumps far from the caseous center. The activity of the AS-D esterase was almost completely inhibited by various trypsin inhibitors, but not by the serine esterase inhibitor of phenylmethylsulfonyl-fluoride. These results suggest that the AS-D esterase is a trypsin-like esterase which participates in the healing of tuberculous lesions.

Animals↗
PubMed1983

Naphthol AS-D chloroacetate esterase reaction. Systematic modifications for optimization to smears and sections.

Currently used methods for naphthol AS-D chloroacetate (NASDCA) esterase reactions can be divided into 3 groups according to the coupler used, i.e., fast garnet GBC, pararosanilin, and new fuchsin. These 3 groups were systematically modified with regard to modes of fixation and incubation in order to develop optimal methods for their application on sections of formalin-fixed and paraffin-embedded tissue as well as blood smears. After obtaining the optimal modifications for each of the 3 coupler methods, comparison was made between the 3 optimal methods for blood smears and the 3 for tissue sections. For blood smears, the methods using either fast garnet GBC or new fuchsin as coupler were found to be equally excellent, while the method using pararosanilin as coupler was not as superior. For tissue sections, the methods using either fast garnet GBC or pararosanilin were found to be equally excellent, while the method using new fuchsin was not as superior. Thus, only the method using fast garnet GBC as coupler was found excellent for both purposes. It was also found that bone marrow biopsies routinely processed through formalin-fixation would yield excellent NASDCA esterase reaction provided Plank and Rychlo's method instead of EDTA was employed for decalcification.

Biopsy↗