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[Effects of processing methods on the amounts of volatile oil of nutmeg and on isolation and characterization of the volatile oil constituents].

In this paper, the authors investigated the effects of various processing methods, i.e., scalding in hot purified talc, simmering wrapped in flour in hot purified talc and stir-frying in smoking wheat bran, on nutmeg (Semen Myristicae) in terms of the quantities of the volatile oil. The experimental results revealed that the amounts of volatile oil contained in nutmeg vary remarkably with the lengths of cooking time and the fluctuation of temperature. Detected by GC-MS-computer, 32 compounds of nutmeg were characterized, and their contents were determined by GC respectively.

Allylbenzene Derivatives

Spectrophotometric determination of certain volatile oils. Part 1: Assay for phenols in volatile oils of clove and thyme.

A differential spectrophotometric method was described for the assay of thyme and clove oils. The method is based on the relative absorbance measurement of the thymate or eugenate anions to the respective molecular thymol or eugenol in alcoholic medium. The results of the differential method, compared with those of the conventional method, are more accurate.

Condiments

[Skin absorption of volatile oils. Pharmacokinetics].

Volatile oils are frequently employed in the local treatment of pain. 1,8-Cineole, the principal component of eucalyptus oil, was used as a model substance to determine whether this active component can be detected in effective amounts at the target area in the skeletal muscles after dermal application. The investigation showed surprisingly large differences depending on the manner of application. The relative bioavailability of the model substance 1,8-cineole obtained by using an applicator was 320% as compared with that obtained by using an occlusive dressing. This has practical effects on the dosage and on the frequency of application.

Administration, Topical

Relaxant effect of the volatile oil of Rosmarinus officinalis on tracheal smooth muscle.

The effects of the volatile oil of Rosmarinus officinalis leaves on the tracheal smooth muscle of rabbit and guinea pig were tested in vitro using isolated tracheal strips. The volatile oil of R. officinalis leaves inhibited the contractions of rabbit tracheal smooth muscle induced by acetylcholine stimulation and the contractions of guinea pig tracheal smooth muscle induced by histamine stimulation. Also, the volatile oil inhibited the contractions of rabbit and guinea pig tracheal smooth muscle induced by high potassium (K+) solution. This inhibition was dose-dependent and reversible. Furthermore, the volatile oil inhibited the contractions of rabbit and guinea pig tracheal smooth muscle induced by acetylcholine and histamine stimulation, respectively, in Ca(2+)-free solution. These data suggest that the volatile oil of R. officinalis leaves has a calcium antagonistic property.

Acetylcholine

[Stability examination of the inclusion compounds of 8 Chinese medicinal volatile oils with beta-cyclodextrin].

The content, hence the stability of volatile oils and their main components in the inclusion compounds of 8 Chinese medicinal volatile oils with beta-cyclodextrin have been examined with different storage times. The 8 Chinese medicines are Vitex negundo var. heterophylla, Notopterygium incisum, Alpinia officinarum, Atractylodes lancea, Pogostenon cablin, Asarum heterotropoides var. mandshuricum, Schizonepeta tenuifolia and Amomum villosum.

Cyclodextrins

[Effects of processing on volatile oil constituents in nutmeg and on the contents of myristicin].

This paper reports a GC analysis of the volatile oil contained in the nutmeg (Semen Myristicae) prepared by simmering wrapped in flour in hot purified talc, scalding in hot purified talc and stir-frying in smoking wheat bran. The experimental results showed that before and after processing the volatile oil compounds in nutmeg are the same and during processing no known compounds disappeared. The contents of the main volatile oil compounds in nutmeg changed, however, before and after processing, including the amounts of myristicin, one of the toxic compounds in nutmeg. It is suggested that the amounts of myristicin could be decreased notably under certain processing conditions.

Allylbenzene Derivatives

[Volatile oil of Anethum Graveolens L. as an inhibitor of yeast and lactic acid bacteria].

The antimicrobial activity of 25 volatile oils from aerial parts and seeds of dill (Anethum graveolens L.) of different geographical origin towards yeast Saccharomyces vini and lactic acid bacteria Lactobacterium buchneri was measured by serial dilutions. Volatile oils from mature seeds and green parts of the plants harvested at late vegetation phases showed the highest activity. The geographical origin of plants influenced insignificantly the antimicrobial activity of volatile oil.

Cell Division

Antibacterial action of essential oils of Artemisia as an ecological factor. I. Antibacterial action of the volatile oils of Artemisia tridentata and Artemisia nova on aerobic bacteria.

Bacterial response to increasing amounts of the volatile oils varies significantly according to species of bacteria tested. Among the four species examined, Escherichia coli was the most resistant to the oils, followed by Neisseria sicca, Bacillus subtilis, and Staphylococcus aureus. The oils of Artemisia tridentata seem to have the same degree of antibacterial action as oils obtained from A. nova.

Animals

Isolation and structure assignment of an antimicrobial principle from the volatile oil of Nigella sativa L. seeds.

Refrigeration of the volatile oil of Nigella sativa L. seeds eventuates in a crystalline substance. The chemical structure of the compound was drawn from its chemical behaviour, as well as from its UV, IR, PMR and mass spectral data. The compound was found to be thymohydroquinone: confirmation of the structure was established via the preparation of its corresponding diacetate ester. The compound was found to have high antimicrobial effect against gram positive microorganisms

Bacillus subtilis

Anticonvulsant activity of the volatile oil from the fruit of Tetrapleura tetraptera.

The anticonvulsant effect of the volatile oil extracted from the fresh fruits of Tetrapleura tetraptera was investigated in mice. The results obtained show that the fresh oil given intraperitoneally offers some protection against leptazol-induced convulsions. A dose of 0.4 ml of the oil per mouse protected 78% of the animals when administered 30 min prior to leptazol. While 0.6 ml offered no protection from death, the onset of convulsions and the average time of death was prolonged.

Africa, Western