[Determination of enzymes and their substrates and reaction products in cell fractions of small intestine carcinoid metastasis and pheochromocytoma].
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The substrate specificity of the dopa decarboxylases of ox adrenal medulla, human phaeochromocytoma and human argentaffinoma have been studied. The enzymes from all three tissues decarboxylated dopa, metatyrosine, orthotyrosine and 5-hydroxytryptophan. Dopa was decarboxylated most rapidly and 5-hydroxytryptophan least rapidly by the enzyme from all three tissues. Competition experiments indicate that all four substrates are decarboxylated by the one enzyme. Attempts to demonstrate decarboxylation of [C(14)]-tyrosine, [C(14)]-tryptophan or [C(14)]-histidine by these enzyme preparations were unsuccessful.
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