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At least 19 recordsLinked to original sources
PubMed2001

Electrochemical characterization of screen-printed carbonaceous electrodes for the determination of peroxidase activity in novel screen-printed flow-through modules.

A novel totally screen-printed flow-through cell for immunoanalysis is presented. It contained screen-printed carbonaceous electrodes, which allowed the determination of peroxidase activity through the electrochemical reduction of p-benzoquinone. As different electrode materials differ strongly in their electrochemical properties, electrodes resulting from various screen-printable carbonaceous pastes were characterized using the hydroquinone/ p-benzoquinone redox couple. For most of the electrodes, cyclic voltammogram peak separations of between 550 and 670 mV were observed indicating only quasi-reversible electrochemical behavior. This was confirmed by variation of the peak separation with scan rate. Heterogeneous electron transfer rates of ca. 0.5 - 1 x 10(-3) cm s(-1) and electrochemical activation energies of ca. 20 kJ mol(-1) were found. These flow-through cells were not only applied to electrochemical peroxidase activity determinations but also, in combination with a separate detector, as affinity reactors. After biotinylation of screen-printed layers, streptavidin and then biotinylated peroxidase could be bound. However, as signals were only 10-20% of those obtained with a column filled with biotinylated glass beads, only the screen-printed electrochemical detector was applied to the detection of antibodies against the African Swine Fever Virus.

Animals↗
PubMed2002

PRINTS and PRINTS-S shed light on protein ancestry.

The PRINTS database houses a collection of protein fingerprints. These may be used to make family and tentative functional assignments for uncharacterised sequences. The September 2001 release (version 32.0) includes 1600 fingerprints, encoding approximately 10 000 motifs, covering a range of globular and membrane proteins, modular polypeptides and so on. In addition to its continued steady growth, we report here its use as a source of annotation in the InterPro resource, and the use of its relational cousin, PRINTS-S, to model relationships between families, including those beyond the reach of conventional sequence analysis approaches. The database is accessible for BLAST, fingerprint and text searches at http://www.bioinf.man.ac.uk/dbbrowser/PRINTS/.

Amino Acid Motifs↗
PubMed2000

PRINTS-S: the database formerly known as PRINTS.

The PRINTS database houses a collection of protein family fingerprints. These are groups of motifs that together are diagnostically more potent than single motifs by virtue of the biological context afforded by matching motif neighbours. Around 1200 fingerprints have now been created and stored in the database. The September 1999 release (version 24.0) encodes approximately 7200 motifs, covering a range of globular and membrane proteins, modular polypeptides and so on. In addition to its continued steady growth, we report here several major changes to the resource, including the design of an automated strategy for database maintenance, and implementation of an object-relational schema for more efficient data management. The database is accessible for BLAST, fingerprint and text searches at http://www.bioinf.man.ac. uk/dbbrowser/PRINTS/

Database Management Systems↗
PubMed1996

[DNA prints instead of plantar prints in neonatal identification].

BACKGROUND: To check the possible usefulness in studying DNA in dried blood spots taken on filter paper blotters for newborn identification. It set out to establish: 1. The validity of the method for analysis; 2. The validity of all stored samples (such as those kept in clinical records); 3. Guarantee of non-intrusion in the genetic code; 4. Acceptable price and execution time. MATERIAL AND METHODS: Forty (40) anonymous 13-year-old samples of 20 subjects (2 per subject) were studied. DNA was extracted using Chelex resin and the STR ("small tandem repeat") of microsatellite DNA was studies using the "polimerase chain reaction method" (PCR). Three non coding DNA loci (CSF1PO, TPOX and THO1) were analyzed by Multiplex amplification. RESULTS: It was possible to type 39 samples, making it possible to match the 20 cases (one by exclusion). The complete procedure yielded the results within 24 hours in all cases. The estimated final cost was found to be a fifth of that conventional maternity/paternity tests. CONCLUSIONS: The study carried out made matching possible in all 20 cases (directly in 19 cases). It was not necessary to study DNA coding areas. The validity of the method for analyzing samples stored for 13 years without any special care was also demonstrated. The technic was fast, producing the results within 24 hours, and at reasonable cost.

DNA Fingerprinting↗
PubMed2003

Patterns and costs of printed and online journal usage.

This study in an academic medical sciences library setting examines the correlation of usage of a matched set of print and online titles, the validity of e-journals usage statistics and the impact of online journals on print journal usage. The print and online usage was determined for 270 journals, both versions of which were available. Print usage was determined annually since 1997 using the reshelving and the error-copies method. Online usage statistics were delivered by five publishers and corrected for redundant multiple accesses. Print journal usage decreased by 22.3 and 30.2% respectively over each of 2 years after the introduction of online journals. Journals published both in print and online lost 30.4% of their print usage within 2 years. The total loss of usage of print-only titles in the same period was somewhat higher, at 45.8%. The average correlation between online and print usage is 0.60 and 0.67 respectively. For the examined titles, users accessed the online versions ten times as often as the print version. Two clearly distinguishable groupings emerged: while with Academic Press and Elsevier, e-journal usage exceeded print usage by a factor of 3 or 4, the e-journals of Blackwell, HighWire and Springer were used on average 14.6 times as frequently as the corresponding print journals. Each usage of a print article cost 2.79-50.82 Euro, each usage of an online article 0.31-15.10 Euro, depending on the publisher. On average, the usage of an online article was 5.4 times cheaper. Within 2-3 years the usage of online journals has outstripped that of print titles by a factor of ten, but the specific spectrum of usage remains much the same as when only print journals alone existed. Print titles not available online suffer a greater decline in usage compared with print/online journals. This confirms that what is read or purchased is determined primarily by ease of access and that there is a steady tendency to reduce the multiplicity of access modes to a manageable few. The availability of journals online seems to have created a new clientele, at least in the case of the German-language Springer journals. The connection between supplier and supply is much less clear with e-journals than it is with print titles. Therefore it is very important to stress and encourage the role of the library as the supplier of this sort of information in the university environment. Collection building issues are discussed in the light of the results.

Costs and Cost Analysis↗