Cuticular lipids of insects. II. Hydrocarbons of the cockroaches Periplaneta australasiae. Periplaneta brunnea and Periplaneta fuliginosa.
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A comparison of behavioral alterations in the brown cockroach, Periplaneta brunnea, and the American cockroach, Periplaneta americana, infected with the acanthocephalan, Moniliformis moniliformis.
We examined behavioral alterations in the brown cockroach, Periplaneta brunnea, infected with the acanthocephalan, Moniliformis moniliformis. The responses of infected and uninfected P. brunnea to light, substrate choice, and activity were compared. Infected brown cockroaches spent more time on white horizontal substrates under red and white light. Infected brown cockroaches also had a shorter freeze time than uninfected cockroaches after being presented with a light source. There was no difference in photophilia or activity of infected and uninfected brown cockroaches. These behavioral alterations in brown cockroaches were compared to those in Periplaneta americana, the American cockroach, infected with the same parasite. American and brown cockroaches differed in some responses to substrate, phototaxis, and activity.
Animals↗
Isolation, characterization and biological activity of a CRF-related diuretic peptide from Periplaneta americana L.
A diuretic peptide (Periplaneta-DP) has been isolated from extracts of whole heads of the cockroach, Periplaneta americana. The purified peptide increases cyclic AMP production and the rate of fluid secretion by isolated Malpighian tubules in vitro. In the fluid secretion assay, the response to native Periplaneta-DP is comparable to that obtained with crude extracts of cockroach corpora cardiaca, and the EC50 lies between 10(-8) and 10(-9) M. The primary structure of Periplaneta-DP was established as a 46-residue amidated peptide: T G S G P S L S I V N P L D V L R Q R L L L E I A R R R M R Q S Q D Q I Q A N R E I L Q T I-NH2. Periplaneta-DP is a further member of the recently established family of CRF-related insect diuretic peptides.
Amino Acid Sequence↗
The nature and development of sex attractant specificity in cockroaches of the genus Periplaneta. I. Sexual dimorphism in the distribution of antennal sense organs in five species.
Sexual dimorphism in the distribution of antennal sense organs is common among adults of the genus Periplaneta. In three out of the four strains of Periplaneta americana examined, adult males had more contact chemoreceptros than females. In the fourth strain of P. americana and in P. australasiae, P. brunnea, P. fuliginosa, and P. japonica, no statistically supportable sexual dimorphism of contact chemoreceptors was found. However, in all strains and species of Periplaneta examined, sexual dimorphism was found in the total number and/or density of olfactory sensilla. Male adults had nearly twice as many olfactory sensilla as female adults. These observations are consistent with the behavioral observation that males within the genus Periplaneta rely on the reception of an airborne pheromone for the initiation of courtship behavior. In P. americana, where sexual dimorphism was found in the contact chemoreceptors, contact stimuli release the full wing raising display and presentation in males during courtship.
Animals↗
Hemolymph transfer as an assay for immunorecognition in the cockroach Periplaneta americana and the locust Schistocerca gregaria.
A quantitative assay for measuring the number of hemocytic nodules formed in response to foreign particles and soluble molecules has been used, in the locust Schistocerca and the cockroach Periplaneta, to investigate the response to transferred hemolymph. Xenogeneic test particles, rabbit neutrophil leukocytes, stimulate formation of nodules when injected into both insect species, compared with saline-injected controls. However, the number of nodules formed in the locust in response to cockroach hemolymph is significantly reduced compared with the response to other xenogeneic cells, and it is suggested that, in view of the strong reactivity of cockroach hemocytes to locust hemolymph and plasma, a graft-versus-host response might be occurring in the recipient locust. Whole hemolymph transferred allogeneically between Periplaneta, or xenogeneically from Blatta to Periplaneta, does not stimulate a response in the recipient. This corresponds well with results from other assays for immunorecognition of transplants and is further confirmation that allogeneic and, in some combinations xenogeneic, recognition is absent in insects.
Animals↗
The nature and development of sex attractant specificity in cockroaches of the genus Periplaneta. IV. electrophysiological study of attractant specificity and its determination by juvenile hormone.
The antennae of male Periplaneta americana acquire a large number of olfactory receptors at the adult stage. Electrophysiological methods (single unit and electroantennogram recording) show that a portion of the receptors added at the adult ecdysis are sex attractant receptors. Sex attractant receptors are not present in large numbers on larval and adult female antennae. The differentiation of pheromone receptors is inhibited during normal larval development by juvenile hormone. Topical application of juvenile hormone-mimic to male antennae during the terminal larval instar inhibits their development. Comparative electrophysiological studies indicate a high degree of cross-reactivity of the P. americana sex attractant among four other species within the genus Periplaneta.
Action Potentials↗
Binding of N-[propionyl-3H]propionylated alpha-bungarotoxin and L-[benzilic-4,4'-3H] quinuclidinyl benzilate to CNS extracts of the cockroach Periplaneta americana.
The nerve cord of the cockroach (Periplaneta americana) contains distinct saturable components of specific binding for the ligands N-[propionyl-3H]propionylated alpha-bungarotoxin and L-[benzilic-4,4'-3H]quinuclidinyl benzilate. N-[Propionyl-3H]propionylated alpha-bungarotoxin bound reversibly to homogenates with a Kd of 4.8 nM and Bmax of 910 fmol mg-1. The association rate constant (1.9 X 10(5) M-1 s-1) and dissociation rate constant (1.2 X 10(-4) s-1) yielded a Kd of 0.6 nM. Nicotinic ligands were found to displace toxin binding most effectively. The binding sites characterized in this way showed many similarities with the properties of the vertebrate neuronal alpha-bungarotoxin binding site. For a range of cholinergic ligands, inhibition constants calculated from toxin binding studies closely corresponded to their effectiveness in blocking the depolarizing response to acetylcholine recorded by electrophysiological methods from an identified cockroach motoneurone. The N-[propionyl-3H]propionylated alpha-bungarotoxin binding component therefore appears to be a constituent of a functional CNS acetylcholine receptor. Binding of L-[benzilic-4,4'-3H]quinuclidinyl benzilate was reversible with a Kd of 8 nM and Bmax of 138 fmol mg-1, determined from equilibrium binding experiments. The Kd calculated from the association rate constant (2.4 X 10(5) M-1 s-1) and dissociation rate constant (1.3 X 10(-4) s-1) was 1.9 nM. Muscarinic ligands were the most potent inhibitors of quinuclidinyl benzilate binding. The characteristics of this binding site resembled those of vertebrate CNS muscarinic cholinergic receptors. In contrast with vertebrate CNS, the nerve cord of Periplaneta americana contains more (approximately X 7) alpha-bungarotoxin binding sites than quinuclidinyl benzilate binding sites.
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Molecular cloning of cDNA for lipopolysaccharide-binding protein from the hemolymph of the American cockroach, Periplaneta americana. Similarity of the protein with animal lectins and its acute phase expression.
A previous paper described the purification of a calcium-dependent lipopolysaccharide-binding protein from the hemolymph of Periplaneta americana (Jomori, T., Kubo, T., and Natori, S. (1990) Eur. J. Biochem. 190, 201-206). This paper describes the molecular cloning and characterization of cDNA for the LPS-binding protein. This protein was found to have a carbohydrate-recognition domain at its carboxyl terminus containing amino acid sequences that are conserved in various mammalian C-type lectins. It was also shown to contain an N-linked carbohydrate chain, and the amino acid residue carrying this chain was assigned as Asn at position 56 (23rd amino acid residue from the amino terminus). Northern blot analysis revealed the presence of multiple mRNAs that hybridized with this cDNA and transient increases in their content after injection of Escherichia coli into adult Periplaneta, suggesting that the LPS-binding protein plays a role in the acute phase response of this insect.
Acute-Phase Proteins↗
Optic lobe neurosecretory cells in Poekilocerus pictus (Orthoptera), Periplaneta americana (Dictyoptera), Belostoma indicum (Hemiptera), Polistes hebraeus (Hymenoptera) and Sandrocottus de jeani (Coleoptera).
Neurosecretory cells in the optic lobes of 5 insects viz. Poekilocerus pictus (Orthoptera), Periplaneta americana (Dictyoptera) Belostoma indicum (Hemiptera), Polistes hebraeus (Hymenoptera) and Sandrocottus dejeani(Coleoptera) have been described. They occur in the distal optic chiasma of the optic lobes in the form of a cone-shaped concentration with two arms, a dorsal and a ventral. The number of neurosecretory cells in the optic lobes varies from species to species viz., about 150 in Poekilocerus, 115 in Periplaneta, 45 in Belostoma, 90 in Polistes and 70 in Sandrocottus. They are mostly monopolar but occasionally bipolar and stain green with PAF and red or sometimes deep purple with Heidenhain's Azan technique. The axons of the monopolar cells run into the optic lobe or the brain, while those of the bipolar cells form a sort of two way connection one end going to the eye and the other to the brain. Neurosecretory cells in all the insects described above have synchronous secretory cycles.
Animals↗
Distribution of serotonin-containing neurons and their pathways in the supraoesophageal ganglion of the cockroach Periplaneta americana (L.) as revealed by immunocytochemistry.
The distribution of serotonin (5-HT)-containing neurons in the supraoesophageal (cerebral) ganglion of the cockroach Periplaneta americana was studied using immunocytochemistry and the formaldehyde histofluorescence method ( Klemm , '83). In this material immunocytochemistry was more sensitive than the formaldehyde histofluorescence procedure. A relatively small number of 5-HT-immunoreactive cell bodies (220-280) were found. For the first time, their processes could be followed. They highly arborize and innervate many brain regions. Three patterns of monoamine innervation have been demonstrated: (1) 5-HT and catecholamine fibres ( Klemm , '83) occurring in the same region (e.g., outer lateral protocerebral neuropil, stratum caudale , mushroom body, fan-shaped body, olfactory lobe), but having certain differences with respect to the organization of their projection fields; (2) 5-HT fibres innervating a region lacking catecholamine-containing fibres (pons); and (3) catecholamine neurons innervating a region lacking 5-HT fibres (ellipsoid body). In the mushroom body only the extrinsic neurons contain 5-HT immunoreactivity. They form a commissural fibre system linking the left- and right-hand mushroom bodies and other brain regions. The pons is part of a 5-HT-neuron fibre system innervating many areas including the mushroom bodies. The present study demonstrates novel, complex, and widely distributed connections within the insect brain.
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Immunocytochemical localization of a methionine-enkephalin-resembling neuropeptide in the central nervous system of the American cockroach, Periplaneta americana L.
Using the peroxidase antiperoxidase immunocytochemical method, we were able to demonstrate within the brain and retrocerebral complex of Periplaneta americana several neuronal structures which were very specifically stained with an anti-methionine-enkephalin antiserum. From the precise localization of this immunoreactive material some speculations about its possible functions could be derived, such as a neurotransmitter- or neuromodulatorlike function and/or a neurohormonal role. These data present new evidence for the recently developed concept that opiate peptides, identical or related to those found in higher species, occur also in invertebrates.
Animals↗
Neurosecretory neurons and their projections to the serotonin neurohemal system of the cockroach Periplaneta americana (L.), and identification of mandibular and maxillary motor neurons associated with this system.
The neuroanatomy of a serotonin neurohemal system in the head of Periplaneta americana was studied by means of immunohistochemistry, cobalt backfilling, transmission electron microscopy, and nerve transection. This neurohemal system is supplied by bilateral groups of two or three neurons whose somata are located ventrally in the subesophageal ganglion, near the root of each mandibular nerve. Axons of these serotoninergic neurons extend into all of the nerves of the mouth parts but reach most of these nerves by a very circuitous route. Initially the axons extend from the subesophageal ganglion, through the ipsilateral mandibular nerve trunk, and into the third branch of the mandibular nerve. From here the axons extend into the second branch of the maxillary nerve by way of a link nerve, and then they project retrogradely to reenter the subesophageal ganglion. In the ganglion, branches of these axons extend into the labial nerves, and the axons run dorsally through the subesophageal ganglion, circumesophgeal connectives, and tritocerebrum to reach the labral nerves. In the nerves of the mouth parts the serotoninergic axons give rise to numerous secondary branches that form an extensive neurohemal system at the surface of these nerves. The relatively large surface and cephalic location of this system probably indicate that the timely release of relatively large amounts of serotonin plays an important role in the physiology of feeding in this insect. The somata, neurites, and dendritic fields of the serotonin neurohemal neurons and those of the motor neurons of the mandibular abductor muscle occur together, and some of the mandibular abductor motor neurons also stain for serotonin. In order to distinguish clearly between these neurohemal and motor neurons, the anatomy of the mandibular abductor motor neurons has also been determined. Similarly, in the course of this study it has been necessary to work out the anatomy of the motor neurons of the maxillary retractor and cardo rotator muscles in order to distinguish them from the serotoninergic neurons. A nonserotoninergic peripheral neuron is associated with the serotonin neurohemal system, and its soma is located on the mandibular-maxillary link nerve. This link nerve neuron appears to be neurosecretory.
Animals↗
Specificity of filiform hair afferent synapses onto giant interneurons in Periplaneta americana: anatomy is not a sufficient determinant.
The synapses between the filiform hair sensory afferents and giant interneurons (GIs) 1-6 of embryonic and first instar cockroaches, Periplaneta americana, were used to investigate the role of neuronal anatomy in determining synaptic specificity. The pattern of afferent-to-GI synapses was first determined by intracellular recording of excitatory postsynaptic potentials (EPSPs). The lateral (L) axon synapses only with GIs 3, 4, and 6, while the medial (M) axon synapses with the contralateral dendrites of all six GIs but with the ipsilateral dendrites only of GIs 1, 2, and 4. The three-dimensional anatomy of the filiform afferents and GIs was determined by injection of cobalt. There is little anatomical segregation of the filiform afferents; consequently, there is no correlation between the anatomy of the GIs and their synaptic inputs. The M axon and ipsilateral GI3 were studied in more detail by light and electron microscopy. Despite the presence of an anterior M axon branch which loops around the ipsilateral GI3 neurite at a distance of 2 microns, no synapses are formed between them. This lack of synapses is not due to the presence of physical barriers. Investigation of filiform afferents and GIs in embryonic ganglia shows that at no stage are the afferents sufficiently separated for their anatomy to be an important factor in determining the specificity of the synaptic inputs of the GIs. It was postulated that two pairs of complementary cell surface labels would be sufficient to code for this specificity, and that, in GIs 3, 5, and 6, spatial differences in the expression of these labels allow the M axon to distinguish ipsilateral dendrites from contralateral.
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Neuronal maps of the frontal ganglion of the cockroach, Periplaneta americana, prepared by heavy metal iontophoresis.
Neurons in whole mount preparations of the frontal ganglion (FG) of the cockroach, Periplaneta americana, were mapped with the aid of cobalt chloride staining and silver intensification techniques. Eighty-six neurons were counted in the FG after staining with reduced methylene blue. The cell size ranged between 20 to 35 microns in diameter. Of the somata located in the FG, 44 were found to contribute their fibers to the nervus recurrens, 26 to the right frontal commissure, 28 to the left frontal commissure, and 6 to the nervus connectivus. In addition, a few neurons presumably from the tritocerebral region also contribute their fibers in the formation of nervus connectivus. The present study has helped delineate the neuronal connections of the FG with the brain and neuroendocrine system (corpora cardiaca and corpora allata). This information will be useful in facilitating the positioning of microelectrodes in our future electrophysiological experiments.
Animals↗
The shadow response of the cockroach periplaneta americana.
We show that the "escape response" (running) of the cockroach Periplaneta americana is interrupted when the insect runs through a shadow. Shadows were cast by holding an opaque plastic sheet so that the cockroach ran beneath it. In control experiments, a similarly positioned clear plastic sheet was ineffective. Removal of the shadow stimulus allowed running to continue. The likelihood that cockroaches would resume running after removal of the shadow stimulus decreased with the time they were allowed to remain in shadow.
Adaptation, Biological↗
Serotonin immunoreactive neurons in the central nervous system of an insect (Periplaneta americana).
Serotonin-like immunoreactivity was mapped in the central nervous system (CNS) of the cockroach, Periplaneta americana. Immunoreactive staining occurred in every ganglion of the CNS. The largest numbers of immunoreactive somata were detected in the optic lobes and the brain, and lowest numbers in the first and second thoracic ganglia. Dense stained fibers, ramifications, and varicosities were found in all ganglia, and numerous axon like processes occurred in all interganglionic connectives. Immunoreactive processes were not, however, detected in most of the peripherally projecting nerve roots. Processes were found only in roots of the suboesophageal ganglion and the tritocerebral lobes of the brain. A comparison of the map for serotonin immunoreactivity with one generated for the pentapeptide transmitter proctolin suggests that the two systems overlap only in the suboesophageal ganglion and the tritocerebrum. The amine and peptide may co-occur in neurons in these regions. The serotonin immunoreactive system appeared significantly different from the octopaminergic system of the ventral nerve cord. Seventy-two potentially identifiable immunoreactive cells were located in the cockroach CNS. Some of these may be suitable for physiological study of the functional role of serotonin.
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A comparison of the escape behaviors of the cockroaches Blaberus craniifer and Periplaneta americana.
A comparison of wind-mediated escape behavior of two species of cockroaches revealed striking differences. In contrast to Periplaneta americana, Blaberus craniifer was unable to escape from a live predator or move away from a synthetically generated puff of wind. Other behavioral differences included degree of preference for areas of low light intensity and propensity to dig and thus bury oneself when disturbed. Anatomical differences, although present, did not seem sufficient to account for the behavioral differences. Also, B. craniifer was able to escape from generated wind puffs but not a live predator when its temperature was raised. These points suggest that the behavioral differences in escape behavior can be attributed to differences in the physiological state of the two nervous systems.
Animals↗
Localization of pancreatic polypeptide (PP)-like immunoreactivity in the central and visceral nervous systems of the cockroach Periplaneta.
The central and visceral nervous systems of the cockroach Periplaneta americana were studied by means of the peroxidase-antiperoxidase immunocytochemical method, with the use of antibody to bovine pancreatic polypeptide (PP). PP-like immunoreactive neuron somata are most numerous in the brain; at least 6 pairs of cell groups occur in clearly defined regions. Three pairs of cells each are also present in the suboesophageal ganglion and the thoracic ganglia, one pair of single cell each in the first abdominal and the frontal ganglia, and 4 to 6 pairs of single cells in the terminal ganglion. No reactive cells were found in the retrocerebral complex and the second to the fifth abdominal ganglia. The axons containing PP-like immunoreactivity issue many branches that are distributed in the entire brain-retrocerebral complex, ventral cord, and visceral nervous system. PP-like immunoreactive material produced in the brain seems to be transported by three routes: protocerebrum to corpora cardiaca (-allata) through the nervi corporis cardiaci, tritocerebrum to visceral nervous system through frontal commissures, and to ventral cord through circumoesophageal connectives. A possible homology between the mammalian brain-GEP (gastro-entero-pancreatic) system and the brain-midgut system of this insect is discussed.
Animals↗