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At least 19 recordsLinked to original sources
PubMed2005

A composite linkage map from two crosses for the species complex Picea mariana x Picea rubens and analysis of synteny with other Pinaceae.

Four individual linkage maps were constructed from two crosses for the species complex Picea mariana (Mill.) B.S.P. x Picea rubens Sarg in order to integrate their information into a composite map and to compare with other Pinaceae. For all individual linkage maps, 12 major linkage groups were recovered with 306 markers per map on average. Before building the composite linkage map, the common male parent between the two crosses made it possible to construct a reference linkage map to validate the relative position of homologous markers. The final composite map had a length of 2,319 cM (Haldane) and contained a total of 1,124 positioned markers, including 1,014 AFLPs, 3 RAPDs, 53 SSRs, and 54 ESTPs, assembled into 12 major linkage groups. Marker density of the composite map was statistically homogenous and was much higher (one marker every 2.1 cM) than that of the individual linkage maps (one marker every 5.7 to 7.1 cM). Synteny was well conserved between individual, reference, and composite linkage maps and 94% of homologous markers were colinear between the reference and composite maps. The combined information from the two crosses increased by about 24% the number of anchor markers compared to the information from any single cross. With a total number of 107 anchor markers (SSRs and ESTPs), the composite linkage map is a useful starting point for large-scale genome comparisons at the intergeneric level in the Pinaceae. Comparisons of this map with those in Pinus and Pseudotsuga allowed the identification of one breakdown in synteny where one linkage group homologous to both Picea and Pinus corresponded to two linkage groups in Pseudotsuga. Implications for the evolution of the Pinaceae genome are discussed.

Chromosome Mapping↗
PubMed2001

The effect of salinity on the emergence and seedling growth of Picea mariana, Picea glauca, and Pinus banksiana.

Mining operations in areas of the boreal forest have caused salinity issues to be a major concern for reclamation. One of the factors determining successful reclamation is the ability of species to self-propagate. The effects of salinity on the seedling emergence and early growth of three boreal forest conifers: Picea mariana, Picea glauca, and Pinus banksiana were determined. Seeds were planted in sand moistened with solutions of various concentrations of sodium chloride or sodium sulfate. Seedling emergence was monitored on a daily basis and growth parameters assessed after 6 weeks. The emergence of Pinus banksiana seedlings was least affected by salinity, and at certain concentrations, emergence even appeared to be stimulated by the presence of salt. Picea glauca was the most sensitive of the species studied. Hypertrophia was observed in all species at high concentrations of Na2SO4, and an increase in salt levels caused a corresponding reduction in seedling height and weight, root length and number of lateral roots.

Environmental Monitoring↗
PubMed2003

Effects of deicing salt on the vitality and health of two spruce species, Picea abies Karst., and Picea glehnii Masters planted along roadsides in northern Japan.

In northern Japan, the growth of Picea abies Karst., and Picea glehnii Masters, which have been planted along the highways, is often suppressed due to several environmental stresses. To examine the adverse effects of deicing salt, the primary source of stress,we measured needle life span, photosynthetic capacity, and water potential and transpiration rate of the two spruce species at a site with damaged trees, near the roadside and a site with healthy trees, located far from the highway. Results from the analysis showed large amounts of sodium and chlorine in the soil and snow at the damaged site. These elements had accumulated in the needles of the spruce. Moreover, physiological traits of the spruce, at the damaged site were also affected. Therefore, we concluded that poor physiological traits might be attributed to an accumulation of deicing salt in the needles, resulting in the suppression of tree growth.

Absorption↗
PubMed2006

Gas exchange and growth responses of ectomycorrhizal Picea mariana, Picea glauca, and Pinus banksiana seedlings to NaCl and Na2SO4.

Black spruce (PICEA MARIANA), white spruce (PICEA GLAUCA), and jack pine (PINUS BANKSIANA) seedlings were inoculated with HEBELOMA CRUSTULINIFORME or LACCARIA BICOLOR and subjected to NaCl and Na (2)SO (4) treatments. The effects of ectomycorrhizas on salt uptake, growth, gas exchange, and needle necrosis varied depending on the tree and fungal species. In jack pine seedlings, ectomycorrhizal (ECM) fungi reduced shoot and root dry weights and in the ECM white spruce, there was a small increase in dry weights. Sodium chloride treatment reduced net photosynthesis and transpiration rates in the three studied tree species. However, NaCl-treated black spruce and jack pine colonized by H. CRUSTULINIFORME maintained relatively high photosynthetic and transpiration rates and needle necrosis of NaCl-treated black spruce seedlings was reduced by the ECM fungi. Higher concentrations of Na (+) were found in shoots compared with roots of the three examined conifer species. ECM fungi reduced the concentrations of Na (+) mainly in the shoots and this reduction was greater in plants treated with NaCl compared with Na (2)SO (4). Shoots contained generally higher concentrations of Cl (-) compared with roots. In the NaCl-treated black spruce and white spruce, both ECM species significantly reduced Cl (-) concentrations. Our results point to overall greater phytotoxicity of NaCl compared with Na (2)SO (4) and support our earlier findings which demonstrated beneficial effects of ECM fungi for woody plants exposed to NaCl stress.

Mycorrhizae↗
PubMed2002

Nuclear DNA content, base composition, heterochromatin and rDNA in Picea omorika and Picea abies.

Two closely related spruces, Picea abies and Picea omorika, a Balkan paleoendemic species, often share habitats, yet never hybridize in nature. The present study adresses their characteristics such as nuclear DNA content, base composition, heterochromatin and rDNA pattern. The genome size of P. abies was 10% larger than that of P. omorika when assessed by flow cytometry, respectively 2C=37.2 pg and 33.8 pg; although when estimated as total chromosome length it was virtually the same. The heterochromatin Chromomycin-A (CMA)/ DAPI fluorochrome banding patterns of both P. abies and P. omorikaare given here for the first time. Simultaneous FISH (fluorescent in situ hybridization) using 18S-26S and 5S rDNA probes revealed 16 18S rDNA sites in P. omorika, 12 18S rDNA sites in P. abies, and a single 5S rDNA locus in both species. The genomes have about 41% GC. The number and position of CMA/DAPI bands and rDNA loci provide good chromosome markers to clarify the karyotypes of the two species.

Journal Article↗
PubMed1993

Polyamines in embryogenic cultures of Norway spruce (Picea abies) and red spruce (Picea rubens).

Embryogenic cultures of red spruce (Picea rubens Sarg.) and Norway spruce (Picea abies (L.) Karst.) were initiated from dissected mature zygotic embryos. The tissues were grown on either proliferation medium or maturation medium. On proliferation medium, the embryogenic tissue continued to produce early stage somatic embryos (organized meristems attached to elongated, suspensor-like cells), whereas on maturation medium fully mature embryos developed from the embryonic tissue. Analysis of polyamines in tissues grown on these two media showed that: (1) both putrescine and spermidine concentrations were always higher in cultures grown on proliferation medium than in cultures grown on maturation medium; (2) in both species, spermidine concentrations declined with time in the tissues grown on maturation medium; and (3) spermine was present in only minute quantities and showed only a small change with time. The presence of difluoromethylornithine in the culture medium had little effect on polyamine concentration, whereas the presence of difluoromethylarginine caused a decrease in putrescine concentrations in both red spruce and Norway spruce tissues grown on proliferation medium or maturation medium.

Journal Article↗
PubMed2001

Desiccation, cryopreservation and water relations parameters of white spruce (Picea glauca) and interior spruce (Picea glauca x engelmannii complex) somatic embryos.

Effects of drying and cryopreservation on survival of spruce (Picea glauca (Moench) Voss and Picea glauca x engelmannii complex) somatic embryos (SEs) were investigated with the aim of developing simple and robust protocols for embryo storage. Somatic embryos dried over salt solutions of known water potential (Psi) survived removal of virtually all free water, to a relative water content (RWC) of approximately 0.13, a value similar to that for spruce zygotic embryos from dry seed. Desiccated SEs also survived subsequent freezing in liquid nitrogen, without the addition of cryoprotectant or pre-culture steps. Highest survival (> 80%) after freezing in liquid nitrogen was in embryos pre-dried to Psi of -15 to -20 MPa, which yielded RWC close to predicted bound (apoplastic) water values. Low (< 35%) or no survival after freezing was observed in embryos pretreated at higher Psi (above -5 MPa) or at very low Psi (-540 MPa, using silica gel), respectively.

Cryopreservation↗
PubMed2006

Impact of eastern dwarf mistletoe (Arceuthobium pusillum) infection on the needles of red spruce (Picea rubens) and white spruce (Picea glauca): oxygen exchange, morphology and composition.

Eastern dwarf mistletoe (Arceuthobium pusillum Peck) is a hemiparasitic angiosperm that infects white spruce (Picea glauca (Moench) Voss) and red spruce (P. rubens Sarg.) in northeastern North America. The effects of mistletoe infection differ substantially between white and red spruce, with white spruce suffering greater infection-induced mortality. In the present study, we sought to determine the role that species-specific differences in needle-scale responses to parasitism may play in the observed differences in the effect of infection on host tree health. Based on the measurements made, the most apparent effect of parasitism was a reduction in needle size distal to infections. The magnitude of this reduction was greater in white spruce than in red spruce. Eastern dwarf mistletoe was a sink for host photosynthate in red spruce and white spruce; however, there were no adjustments in needle photosynthetic capacities in either host to accommodate the added sink demands of the parasite. Needle total nonstructural carbohydrate concentrations (TNC) were also unaltered by infection. Red spruce needles had higher TNC concentrations despite having lower overall photosynthetic capacities, suggesting that red spruce may be more sink limited and therefore better able to satisfy the added sink demands of parasitic infection. However, if carbon availability limits the growth of the mistletoe, one may expect that the extent of the parasitic infection would be greater in red spruce. Yet in the field, the extent of infection is generally greater in white spruce. Taken together, these results suggest that dwarf mistletoe may not substantially perturb the carbon balance of either host spruce species and that species-specific differences in needle-scale responses to the parasite cannot explain the contrasting effects of infection on white spruce and red spruce.

Carbohydrates↗
PubMed1989

Ozone flux to Picea sitchensis (Bong) Carr and Picea abies (L) Karst during short episodes and the effects of these on transpiration and photosynthesis.

Sitka spruce and Norway spruce were grown in controlled environments and then exposed to ozone (O3) for short periods as in mid-afternoon episodes experienced in the forest. For concentrations of between 20 and 300 nl litre(-1) there were linear relationships between exposure concentration and O3 uptake rates. Increasing photon flux densities increased rates of photosynthesis and transpiration, the increases being larger in actively growing than dormant seedlings. Physiological condition (dormancy or active growth), species and photon flux density were found to influence O3 flux via their effects on stomatal conductance. Exposure to 80 nl litre(-1) O3 resulted in consistent increases of stomatal conductance and there were also indications that water-use efficiency was decreased.

Journal Article↗
PubMed2001

The role of sucrose during maturation of black spruce (Picea mariana) and white spruce (Picea glauca) somatic embryos.

The present study was conducted to understand the role of sucrose in the medium on the maturation of black spruce and white spruce somatic embryos. A maturation medium containing 6% sucrose, which hydrolyzed into glucose and fructose, gave significantly more embryos than a medium containing 3.16% of each glucose and fructose. Preventing the complete sucrose hydrolysis by a daily transfer of the tissues onto fresh medium significantly decreased the yield of somatic embryos compared to when sucrose was allowed to complete its hydrolysis. This reduction was not due to the manipulation of the tissues during the transfer, since a daily in situ transfer did not affect embryo production. To verify if the better embryo production observed on a medium containing 6% sucrose was due to the increasing osmotic pressure of the medium, this increasing osmotic pressure was simulated with a sequence of media containing different concentrations of glucose and fructose. Unexpectedly and for both species, this simulation did not improve somatic embryo production, which stayed similar to the one obtained on constant osmotic pressure. To understand these results, embryos produced on the different treatments were analyzed in terms of sucrose, glucose, fructose and starch levels and protein contents. The embryo carbohydrate content was independent from the carbohydrate used in the maturation medium. However, embryos matured on 6% sucrose allowed to hydrolyze during the maturation period contained significantly more soluble and insoluble proteins than embryos matured on any other treatment. Furthermore, embryos with a higher protein content also exhibited a higher epicotyl appearance frequency. The role of sucrose as a regulatory factor during the maturation of spruce somatic embryos is discussed.

Journal Article↗
PubMed2003

[Eco-physiological responses of Picea mongolica ecotypes to drought stress].

The seedlings of different Picea mongolica ecotypes were treated with drought stress, and their height growth, MDA, proline, and chlorophyll content were investigated. The results showed that the height growth was obviously reduced by drought stress. With sufficient water supply, the height growth of f. viridis W. D. Xu f. nov Picea mongolica was rapid and obviously better than that of f. purpurea W. D. Xu f. nov Picea mongolica, but was obviously reduced by intense drought stress. Furthermore, drought stress shortened the annual growth period of Picea mongolica, and made its terminal bud formation more than one month earlier increased in both needles and roots, but was higher in needle than in root. The MDA content was higher in Picea mongolica than in f. viridis W. D. Xu f. nov Piburcea mongolica, and the proline content in needles was increased with increasing drought stress, but in roots, the proline content was higher in f. viridis W. D. Xu f. nov Picea mongolica than in f. purpurea W. D. Xu f. nov Picea mongolica and f. rubra W. D. Xu f. nov Picea mongolica. The latter 2 forms showed a similar trend in chlorophyll content. Their chlorophyll a, chlorophyll b and total chlorophyll contents increased at the beginning of drought treatment, and then decreased with increasing drought stress. The chlorophyll content of f. viridis W. D. Xu f. nov Picea mongolica decreased with drought stress. The ratio of chlorophyll a/b decreased from maximum to minimum in f. viridis W. D. Xu f. nov Picea mongolica, but decreased in a relative small extent in f. purpurea W. D. Xu f. nov. It's proved that f. purpurea W. D. Xu f. nov Picea mongolica had a larger drought-resistance capacity than f. viridis W. D. Xu f. nov, and f. rubra W. D. Xu f. nov was a typical transitional ecotype from f. purpurea W. D. Xu f. nov to f. viridis W. D. Xu f. nov.

Chlorophyll↗
PubMed2004

[Soil water characteristics in Picea crassifolia forestry lands in Qilian Mountains].

The study of soil water content and its main affecting factors in Picea crassifolia forestry lands in Qilian Mountains showed that the Picea crassifolia forestry lands were characterized by lower soil bulk weight and higher soil water infiltration rate, and the vertical distribution of soil water was apparently affected by precipitation. The soil bulk weight of shrub-Picea crassifolia forestry land, moss-Picea crassifolia forestry land and meadow land was 0.522, 0.641 and 0.874 g x cm(-3), initial water infiltration rate was 6.0, 21.0 and 2.5 mm x min(-1), and stable infiltration rate was 3.98, 11.2 and 0.5 mm x min(-1), respectively. The soil of moss-Picea crassifolia land in 100 cm depth could be partitioned into 4 layers, i.e., active layer (0 - 30 cm), regulation layer (30 - 60 cm), transmission layer (60 - 80 cm) and accumulation layer (below 80 cm), and the amount of stored water in each layer was 3.30, 3.17, 2.80 and 2.83 mm x cm(-1), respectively. The soil of shrub-Picea crassifolia land could be partitioned into 3 layers, i.e., stable layer (0 - 30 cm), active layer (30 - 60 cm) and transmission layer (below 60 cm), and the amount of stored water in each layer was 3.98, 3.65 and 3.48 mm x cm(-1), respectively. The seasonal variance of soil water content in Picea crassifolia forestry lands was strongly affected by precipitation. The water holding capacity of shrub-Picea crassifolia forestry land was higher and the water transmission capability was lower than that of Moss-Picea crassifolia forestry land.

China↗
PubMed2005

Nuclear ribosomal DNA internal transcribed spacer 1 (ITS1) in Picea (Pinaceae): sequence divergence and structure.

The nrDNA ITS1 of Picea is 2747-3271 bp, the longest known of all plants. We obtained 24 cloned ITS1 sequences from six individuals of Picea glehnii, Picea mariana, Picea orientalis, and Picea rubens. Mean sequence divergence within these individuals (0.018+/-0.009) is more than half that between the species (0.031+/-0.011) and may be maintained against concerted evolution by separation of Picea 18S-26S rDNA repeats on multiple chromosomes. Picea ITS1 contains three subrepeats with a motif (5'-GGCCACCCTAGTC) that is conserved across Pinaceae. Two subrepeats are tandem, remote from the third, and more closely related and significantly more similar to one another than either is to the third subrepeat. This correlation between similarity and proximity may be the result of subrepeat duplication or concerted evolution within rDNA repeats. In inferred secondary structures, subrepeats generally form long hairpins, with a portion of the Pinaceae conserved motif in the terminal loop, and tandem subrepeats pair with one another over most of their length. Coalescence of ITS1 sequences occurs in P. orientalis but not in the other species.

Base Sequence↗
PubMed2001

Mean canopy stomatal conductance responses to water and nutrient availabilities in Picea abies and Pinus taeda.

We compared sap-flux-scaled, mean, canopy stomatal conductance (GS) between Picea abies (L.) Karst. in Sweden and Pinus taeda (L.) in North Carolina, both growing on nutritionally poor soils. Stomatal conductance of Picea abies was approximately half that of Pinus taeda and the sensitivity of GS in Picea abies to vapor pressure deficit (D) was lower than in Pinus taeda. Optimal fertilization increased leaf area index (L) two- and threefold in Pinus taeda and Picea abies, respectively, regardless of whether irrigation was increased. Although it increased L, fertilization did not increase GS in Picea abies unless irrigation was also provided. In Pinus taeda growing on coarse, sandy soils, the doubling of L in response to fertilization reduced GS sharply unless irrigation was also provided. The reduction in GS with fertilization in the absence of irrigation resulted from the production of fine roots with low saturated hydraulic conductivity. When Pinus taeda received both fertilization and irrigation, the increase in L was accompanied by a large increase in GS. In Pinus taeda, a reference GS (defined as GS at D = 1 kPa; GSR) decreased in all treatments with decreasing volumetric soil water content (theta). In Picea abies, theta varied little within a treatment, but overall, GSR declined with theta, reaching lowest values when drought was imposed by the interception of precipitation. Despite the large difference in GS both between Picea abies and Pinus taeda and among treatments, stem growth was related to absorbed radiation, and stem growth response to treatment reflected mostly the changes in L.

Biomass↗
PubMed2003

[Gradient distribution of soil N, P, and K along the distance from Picea mongolica seedlings root surface].

Six years old seedlings of Picea mongolica were planted in pots with horizontal and vertical root treatments. Soil samples were stratified collected, and their available nitrogen (N), phosphorus (P), and potassium (K) contents were determined. The results showed that the available contents of N, P and K in soils along the distance from Picea mongolica seedlings root surface had a special gradient distribution. Near root surface, there was a depletion zone of soil available N, P, and K, and along the distance from it, the nutrient element contents increased gradually and reached their background values in the bulk soil. In the horizontal root treatment, the depletion zone of soil available N, P, and K existed in different distance from root surface, owing to the absorption and the use intensity of the seedlings to these nutrient elements. The depletion zone of soil available N and K appeared from 1 cm from root surface, and that of available P existed in 0.5 cm from root surface. In the vertical root treatment the depletion zone of available N and K also appeared from 1 cm from root surface as in the horizontal root treatment, but the available P content near root surface was lower than that far away from root surface. It is proved that the absorption and the utilize intensity of soil available P by Picea mongolica seedlings was intense, which might become a limiting factor to its growth and development. Therefore, Picea mongolica should be planted on soils rich in phosphorus, or P and N fertilizers should be applied to improve the absorption and the use efficiency of available P by Picea mongolica.

Nitrogen↗
PubMed2005

[Effect of ectomycorrhizae on the growth of Picea koraiensis seedlings].

Basidioscarps of Agaricales in different Picea koraiensis forest plantations were collected during August-October, 2000. 36 isolaters of species of Agaricales were obtained by isolating and culturing to the basidioscarps. Through indoor inoculation test on seedlings of Picea koraiensis, 6 ectomycorrhizae fungi cultures were obtained from 36 isolaters. The inoculation results show that the period for ectomycorrhizae inoculation to 1-year seedlings of Picea koraiensis should be about 30 days after seedlings emerging, the suitable temperature for ectomycorrhizae forming is about 20 degrees C. 6 ectomycorrhizae strains all have growth-promoting effect to the seedlings of Picea koraiensis. The contents of chlorophyll a of the seedlings inoculated strains of Agaricus silvaticus, 031 and L15 were significantly higher than other strains and control. The contents of chlorophyll b in the seedlings inoculated strains 009, 004, Agaricus silvaticus and L15 were significantly higher than other strains and control. The weights of seedlings which inoculated strains 009, 025, 031, Agaricus silvaticus and L15 were significantly different to control, the weight of seedlings inoculated strains of Agaricus silvaticus and L15 are 19.23% and 23.08% more than control; The heights of the seedlings inoculated 6 strains all have significant difference to control, the weight of seedlings inoculated strains of Agaricus silvaticus and L15 are 17.83% and 16.37% more than control. The results of outdoor inoculation show that the seedlings inoculated Agaricus silvaticus grow best on height, 9.25% more than control after inoculated 70 days; the seedlings inoculated strain L15 grow best on collar diameter, 9.92% more than control after inoculated 70 days; the lateral root numbers of seedlings inoculated strain 009 is largest, 51.91% more than control after inoculated 70 days; the main roots of seedlings inoculated strain 009 are longest, 3.36% more than control after inoculated 70 days; the ectomycorrhizae fungi infestation percentage of seedlings inoculated strain 031 is highest, 13.90% more than control after inoculated 70 days; the photosynthesis of seedlings inoculated strain 031 is more powerful, 20.02% more than control after inoculated 70 days. To sum up, strain 009, strain 031 and strain L15 should be fine strains to seedlings of Picea koraiensis.

Biomass↗
PubMed2006

Comparative genome mapping among Picea glauca, P. mariana x P. rubens and P. abies, and correspondence with other Pinaceae.

A composite linkage map was constructed from four individual maps for the conifer Picea glauca (Moench) Voss, from anonymous and gene-specific markfers (714 AFLPs, 38 SSRs, and 53 ESTPs). A total of 12 linkage groups were delineated with an average marker density of 2.7 cM. Macro-synteny and macro-colinearity comparisons with two other composite linkage maps developed for the species complex P. mariana (Mill.) B.S.P. x P. rubens Sarg., and for P. abies (L.) Karst. revealed an identical number of linkage groups and a remarkable conservation of the gene content and gene order of linkage groups over the million years since the split between these taxa. Identical gene order among taxa was observed for 10 of the 12 assembled composite linkage groups. The discovery of one breakdown in synteny between P. glauca and the other two taxa indicated the occurrence of an inter-chromosomal rearrangement involving an insertional translocation. Analysis of marker colinearity also revealed a putative segmental duplication. The combined information from these three Picea genomes validated and improved large-scale genome comparisons at the inter-generic level in the family Pinaceae by allowing for the identification of 11 homoeologous linkage groups between Picea and Pinus, and nine such groups between Picea and Pseudotsuga menziesii. Notably, the analysis of synteny among the three genera revealed a putative case of chromosomal fission and an inter-chromosomal rearrangement in the genome of P. menziesii. Both of these changes are inter-connected, indicating much instability in this part of the P. menziesii genome. Overall, the macro-structure of the Pinaceae genome was well conserved, which is notable given the Cretaceous origin of its main lineages.

Genome, Plant↗