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Two Saccharopolyspora isolates from archaeological excavation sites: polyphasic taxonomy, biosynthetic potential, bioactivity profiling and description of Saccharopolyspora antiqui sp. nov.

Archaeological excavation sites represent underexplored microbial habitats with the potential to recover taxonomically and biotechnologically valuable actinomycetes. In this study, two Saccharopolyspora strains, 5N708T and 5N102, were isolated from soil samples collected from the Gaziantep-Doliche-Dülük and Bitlis-Ahlat-Selçuklu Cemetery archaeological excavation sites in Türkiye. A polyphasic taxonomic approach, including 16S rRNA gene sequencing, phylogenetic and phylogenomic analyses, average nucleotide identity, digital DNA-DNA hybridization, phenotypic characterization, and chemotaxonomic analyses, showed that strain 5N708T represents a novel species of the genus Saccharopolyspora, for which the name Saccharopolyspora antiqui sp. nov. is proposed, whereas strain 5N102 was assigned to Saccharopolyspora elongata. Both isolates were further evaluated for their antimicrobial, antioxidant, and cytotoxic activities, and their biosynthetic potential was investigated by genome mining. Both strains showed activity against Staphylococcus aureus, with strain 5N708T producing the larger inhibition zone. Strain 5N102 exhibited markedly stronger antioxidant activity than strain 5N708T in radical scavenging, ferric reducing antioxidant power, and reducing power assays. In contrast, strain 5N708T showed more promising cytotoxic activity, with relative selectivity toward MIA PaCa-2 pancreatic cancer cells compared with HEK293 cells after prolonged incubation. Genome mining revealed multiple biosynthetic gene clusters in both isolates, supporting their capacity to produce secondary metabolites. These findings indicate that archaeological soils are promising reservoirs of taxonomically novel and biologically active Saccharopolyspora strains.

Saccharopolyspora

Rhodococcus dendrobeaniae sp. nov., an actinomycete isolated from an Arctic marine invertebrate exhibiting cytotoxic activity, and an emended description of Rhodococcus sovatensis.

A polyphasic study was conducted to establish the taxonomic status of strain T060T, an orange, aerobic, coccoid and non-motile actinomycete, isolated from a marine bryozoan (Dendrobeania sp.) collected in the Barents Sea. Phylogenetic analysis of the 16S rRNA gene sequences revealed Rhodococcus sovatensis DSM 102881T as the closest related species to strain T060T with a similarity of 99.54%. Phylogenomic analysis confirmed a close relationship between T060T and R. sovatensis DSM 102881T, while supporting their distinction. Digital DNA-DNA hybridization and average nucleotide identity values between strain T060T and R. sovatensis DSM 102881T were 26.4 and 84.3%, respectively, supporting the delineation of the isolate as a new species. Genomic characterization of the assembled genomes of T060T and R. sovatensis DSM 102881T showed genome sizes of 5.3 and 4.2 Mbp, with a G + C content of 64.38 and 65.01%, respectively. Genome analysis of strain T060T identified 15 biosynthetic gene clusters (BGCs) with low sequence similarity to known BGCs, indicating its capacity to produce unknown, potentially bioactive secondary metabolites. Furthermore, growth of T060T in eight different media revealed condition-dependent cytotoxic activity. The strongest cytotoxicity was observed for fractionated extracts from T060T grown in half-strength ISP 2 with filtered seawater against the human malignant cell lines MCF7 and A2058, and to a lesser extent against the non-malignant MRC5 cell line, highlighting its biotechnological potential. Based on the data from polyphasic taxonomy studies, it is proposed that strain T060T be classified in the genus Rhodococcus as Rhodococcus dendrobeaniae sp. nov. Additionally, the acquired data of R. sovatensis were used to amend its original description.

Rhodococcus

Halosimplex yunnanense sp. nov., a novel haloarchaeon from an underground salt mine.

Strain J119T, a halophilic archaeon, was isolated from a salt mine sample collected in Yunnan Province, China. Cells are spherical (diameter 0.5-0.7 µm) or short‑rod‑shaped (0.4-0.5 × 0.7-0.8 µm), non‑motile, Gram‑stain‑negative, and can grow at 20-55°C (optimum 42°C), with NaCl tolerance ranging from 15% to 30% (w/v) (optimum 20%) and a pH growth range of 5.5-9.0 (optimum pH 7.0). Strain J119T's nearly complete 16S rRNA gene sequence (1,452 bp; accession MW736888.1) shows the highest sequence similarity (97.32%) to Halosimplex salinum YPL4T; this value is lower than the species boundary threshold of 98.65%. Its rpoB' gene (1,830 bp; NZ_JBTJEL000000000.1) shares the highest similarity (95.52%) with Halosimplex aquaticum XZYJT29T. Genomic analyses revealed that the average amino acid identity, average nucleotide identity and digital DNA-DNA hybridization values between strain J119T and strains YPL4T and XZYJT29T were 78.58%, 82.59%, 25.50% and 82.61%, 85.70%, 29.20%, respectively. The genomic DNA G + C content of strain J119T is 66.5%. Phenotypic, phylogenetic, and genome-based analyses suggest that strain J119T (= KCTC 4326T = MCCC 4K00178T) represents a novel species of the genus Halosimplex, for which the name Halosimplex yunnanense sp. nov. is proposed.

RNA, Ribosomal, 16S

Salinimicrobium molybdatiresistens sp. nov., a novel molybdate-resistant and selenite-reducing bacterium isolated from river silt.

Strain TH3T was isolated from the river bottom silt collected in Hengshui, Hebei Province, China. The bacterium is a yellow-pigmented, rod-shaped, Gram-staining negative and aerobic organism. It was able to grow between 10 and 37 °C (optimum 30 °C), at pH values from 5.0 to 9.0 (optimum pH 7.0), and tolerated NaCl concentrations ranging from 0 to 13% (w/v, optimum 2%). The 16 S rRNA gene sequence of strain TH3T was found to be most closely related to Salinimicrobium sediminilitoris ASW11-47T (99.7%). Nevertheless, genome comparison revealed the relatedness indices below species delineation thresholds: digital DNA-DNA hybridization was 49.7%, and average nucleotide identity was 93.2%, and average amino acid identity was 94.2% compared to Salinimicrobium sediminilitoris ASW11-47T. Strain TH3T had a genome size of 3.7 Mb and a DNA G + C content of 41.0%. The major fatty acids observed for strain TH3T (≥ 5%) were iso-C14:0, iso-C15:0, anteiso-C15:0, iso-C16:0, iso-C16:0 3-OH, iso-C17:0 3-OH, and summed feature 3. The polar lipid composition included phosphatidylethanolamine, one unidentified phospholipid, two aminolipids, along with five unknown lipids. The sole respiratory quinone in strain TH3T was menaquinone-6. In addition, strain TH3T was highly resistant to molybdate (500 mM) and selenite (20 mM), and could completely reduce 1 mM selenite to red elemental SeNPs within 3 d. Strain TH3T contains several putative selenite-reducing genes, including sodA, serA, serC, cysH, deoC, tktA, and pdhC. Based on polyphasic characterization, strain TH3T was found to be a novel species in the genus Salinimicrobium, and the proposed name is Salinimicrobium molybdatiresistens sp. nov. The type strain is TH3T (= GDMCC 1.3399T = JCM 35713T).

Rivers

Isolation and characterization of two novel species Neorhizobium fuzhouense sp. nov. and Neotabrizicola paludis sp. nov.

Two novel aerobic bacterial strains, designated SGZ-38T and sgz301269T, were isolated from the root of Pennisetum sp. and paddy soil, respectively. Strain SGZ-38T grew at 10-40 ℃ (optimum 30 °C) and pH 5.0-12.0 (optimum 6.5) and tolerated up to 1.0% NaCl (w/v), whereas strain sgz301269T grew at 15-37 °C (optimum 30 °C), pH 5.0-9.5 (optimum 7.0) and 0-2% NaCl (optimum 0%). Phylogenetic trees based on the 16S rRNA gene and genomes placed both strains into distinct lineages, forming separated clades from their closest relatives. Strain SGZ-381T exhibited the highest 16S rRNA gene similarities to "Neorhizobium deserti" ACCC 61627T (97.4%), and strain sgz301269T had the highest 16S rRNA gene sequence similarity to Neotabrizicola shimadae N10T (97.6%). The respiratory quinone in both strains was ubiquinone-10. The main fatty acids of SGZ-381T were Summed feature 8, Summed feature 2 and C16:0, whereas strain sgz301269T included C10:0 3OH, C18:0 3OH and Summed feature 8. The DNA G+C content of SGZ-381T and sgz301269T was 62.1% and 65.5%, respectively. The average nucleotide identity and digital DNA-DNA hybridization values between each strain and their respective closest species were 74.6% and 20.1%, 75.3% and 17.4% respectively, below the thresholds for species delineation. Based on the comprehensive chemotaxonomic, phylogenetic, and phenotypic evidence, proposed names of the novel strains are Neorhizobium fuzhouense sp. nov. (type strain SGZ-381T=GDMCC1.4207T=JCM 36770T), Neotabrizicola paludis sp. nov. (type strain sgz301269T=MCCC 1K09178T=KCTC 8856T).

Bacterial Typing Techniques

Albidovulum molybdatiresistens sp. nov., a molybdate-resistant bacterium isolated from river water.

A Gram-stain-negative, aerobic, non-motile, catalase- and oxidase-positive, white rod-shaped strain, RF13T, was isolated from water samples of the Qingliang River in Fucheng County, Hebei Province, China, and was grown at 15-42 °C (optimum 35 °C), pH 6.0-8.0 (optimum pH 7), and 0-0.5% (w/v) NaCl (optimum concentration 0%). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain RF13T belonged to the genus Albidovulum, with closest sequence similarity to Albidovulum salinarum MCCC 1K0602T (97.2%), Frigidibacter oleivorans CGMCC 1.3778T (97.2%), Allgaiera indica MCCC 1A01802T (96.8%), and Pseudothioclava arenosa KCTC 52190T (96.4%). The genome size of strain RF13T was 3.7 Mb, and the DNA G+C content was 64.6%. The DNA-DNA hybridisation value (dDDH), average nucleotide identity (ANI), and average amino acid identity (AAI) between strain RF13T and the reference strain were less than 20.0%, 78.8%, and 72.8%, respectively. Chemotaxonomic analysis revealed Summed feature 8 (48.4%) (C18:1 ω6c and/or C18:1 ω7c), C18:1 ω7c 11-methyl (22.1%), C18:0 3OH (7.9%), and C10:0 3OH (5.0%) as predominant fatty acids. The polar lipids consisted of phosphatidylglycerol, diphosphatidylglycerol, two unidentified aminolipids, two unidentified phospholipids, and three unidentified lipids. The predominant isoprenoid quinone was ubiquinone-10 (Q-10), and a small amount of Q-9 was also detected. In addition, strain RF13T exhibited a minimum inhibitory concentration (MIC) of 20 mM for molybdate in R2A broth medium and was capable of reducing molybdate to molybdenum blue. Based on the results of biochemical, physiological, phylogenetic, and chemotaxonomic analyses, combined with 16S rRNA gene sequence analyses and draft genome sequence comparisons, strain RF13T was considered to represent a novel species of the genus Albidovulum, and was therefore named Albidovulum molybdatiresistens sp. nov. The type strain was RF13T (= GDMCC 1.3414T= JCM 35643T).

Phylogeny

Whole genome-based reclassification of the genus Metabacillus: Proposal for five novel genera, Chryseobacillus gen. nov., Cohnibacillus gen. nov., Salimetabacillus gen. nov., Pantoeobacillus gen. nov., and Lutimetabacillus gen. nov. and the description of one novel bacterial species, Chryseobacillus diguaensis sp. nov. isolated from soil in the Digua reservoir.

Comprehensive phylogenomic and comparative genomic analyses were conducted to clarify the taxonomic boundaries of the genus Metabacillus. Phylogenetic trees reconstructed from a set of single-copy orthologous proteins (SCOPs) revealed that the genus, as currently defined, is polyphyletic. The type species of the genus Metabacillus and its closest relatives formed a consistent clade, herein designated as Metabacillus sensu stricto. The remaining species were grouped into three well-supported clades: Kandeliae, Indicus, and Mangrovi, and two single-taxon lineages: M. arenae and M. lacus. The phylogenomic delineation found in these divergent taxa was corroborated by either inconsistent distribution patterns or the absence of previously defined conserved signature indels (CSIs) specific to Metabacillus. Genomic metrics, including Average Nucleotide Identity (ANI), Average Amino acid Identity (AAI), and digital DNA-DNA hybridization (dDDH) further supported the taxonomic delineation proposed here. The observed genomic divergence was mirrored by phenotypic differences, including variations in GC content ranges. Based on this polyphasic evidence, we propose the reclassification of the genus Metabacillus taxa into five novel genera: Chryseobacillus gen. nov. (encompassing the Kandeliae clade), Cohnibacillus gen. nov. (M. lacus), Salimetabacillus gen. nov. (M. arenae), Pantoeobacillus gen. nov. (Indicus clade), and Lutimetabacillus gen. nov. (Mangrovi clade). The core lineage is retained as Metabacillus sensu stricto, for which an emended description of the genus Metabacillus is also provided. A novel bacterial strain, designated as MAU-250T, was isolated from a soil sample collected on the shore of an artificial reservoir in the Andean foothills of the Maule Region in central Chile. Public metagenome screening supported a low-abundance taxon with broad ecological adaptability, preferentially associated with soil habitats. A polyphasic analysis based on phenotypic traits and genomic distances (78.0% ANIb and 19.8% dDDH against its closest relative) also supported its designation as a novel species, for which the name Chryseobacillus diguaensis sp. nov. is proposed. The type strain is MAU-250T (=RGM 3146T = IMI 507634T).

Phylogeny

Description and genomic characterization of Aquipuribacter aurantiacus sp. nov., isolated from saline lake sediment.

Strains MA13-6T and MA13-13, two Gram-stain-positive, aerobic, short rod-shaped actinobacteria, were isolated from a saline lake in Ngari Prefecture, Xizang Autonomous Region, China. Phylogenetic analysis based on 16S rRNA gene sequences indicated that these two strains belonged to the genus Aquipuribacter, with the closest relationship to Aquipuribacter hungaricus IV-75T (98.47% sequence similarity) and Aquipuribacter nitratireducens AMV4T (97.36% sequence similarity). Phylogenetic analysis based on genomes further confirmed their classification as a distinct cluster within the genus Aquipuribacter. The average nucleotide identity and digtal DNA-DNA hybridization values between these two strains and their closest relative Aquipuribacter hungaricus IV-75T, were 82.44-82.49% and 23.00%, respectively, clearly indicating that strains MA13-6T and MA13-13 represent a novel species. The 16S rRNA gene sequence similarity, average nucleotide identity and digital DNA-DNA hybridization values between these two strains were 99.79%, 99.97% and 99.40%, respectively, unequivocally confirming their classification within the same species. However, DNA fingerprinting analysis distinguished them as non-clonal variants. The polar lipids comprised phosphatidylglycerol, two unidentified phospholipids, two unidentified glycolipids, and two unidentified lipids. The predominant respiratory quinone was MK-10 (H4). The major fatty acids were anteiso-C15:0, C18:1ω9c, isoC16:0 and anteiso-C17:0. The cell wall diagnostic diamino acid was meso-diaminopimelic acid. Based on phylogenetic analyses combined with phenotypic and chemotaxonomic characterization, strains MA13-6T and MA13-13 represent a novel species of the genus Aquipuribacter, for which the name Aquipuribacter aurantiacus sp. nov. is proposed. The type strain is MA13-6T (=MCCC 1K10045T = KCTC 59572T).

Phylogeny

Rhodococcus folensis sp. nov., an orange-red-pigmented bacterium from mining soil.

Mining-impacted environments represent chemically complex ecosystems that may harbor metabolically versatile and pigment-producing microorganisms. During a survey of pigment-producing bacteria from abandoned mining soil in Trabzon, Türkiye, a red-pigmented strain, designated FMA22T, was isolated and characterized using a polyphasic taxonomic approach. 16 S rRNA gene sequence analysis placed the strain within the genus Rhodococcus, showing the highest similarity to R. corynebacterioides DSM 20,151T (99.57%), R. kroppenstedtii DSM 44908ᵀ (99.06%) and R. trifolii T8T (98.96%). The strain was Gram-stain-positive, aerobic and non-motile, and grew at 4-40 °C. Polar lipids included phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside, phosphatidylcholine, five unidentified glycolipids, four unidentified lipids, one unidentified phospholipid and one unidentified phosphoglycolipid; MK-8(H2) was the major respiratory quinone. Major fatty acids were C18:1 ω9c, summed feature 3 (C16:1 ω7c/C16:1 ω6c) and C16:0. ANI and dDDH values with the closest relatives were below 76.8% and 20.5%, respectively. The draft genome (4.23 Mb; 67.2 mol% G + C; 4,106 CDSs) harbors a terpene-associated carotenoid cluster containing crtB, crtI and crtY. The orange-red pigment (λmax = 475 nm) showed antioxidant activity (DPPH SC₅₀ = 5.38 mg mL⁻¹; FRAP = 4.34 µmol TE g⁻¹) and weak but measurable HIV-1 reverse transcriptase inhibition (IC₅₀ = 22 mg mL⁻¹). These data support the proposal of Rhodococcus folensis sp. nov., with FMA22ᵀ (= LMG 34144ᵀ = DSM 120048ᵀ) as the type strain.

Soil Microbiology

Aggregicoccus is a myxobacterial genus inherently deficient in fruiting genes.

Myxobacteria are fascinating and important prokaryotes with remarkable multicellular behaviors, which make them a model system for studying prokaryotic development and cooperation. Although there have been sporadic discoveries of myxobacterial species unable to fruit, it is unclear whether the non-fruiting characteristic is due to taxon-specific genetic deficiency or suboptimal cultivation conditions. Aggregicoccus is a non-fruiting myxobacterial genus typified by a single validly published species, Ag. edonensis. In this study, we report five novel Aggregicoccus strains, which are classified into three novel type species, Ag. lacus, Ag. agri, and Ag. guangxiensis, based on polyphasic taxonomic analysis. All the Aggregicoccus strains are unable to produce fruiting bodies, but can still sporulate. We compared the genome differences between Aggregicoccus and Myxococcus; both genera belong to the Myxococcaceae family, and all the genomes are of similar sizes. The results showed that the Aggregicoccus strains are inherently deficient in the fruiting body-associated genomic information (FAGI). We propose an assessment of FAGI for the classification of non-fruiting myxobacterial species.IMPORTANCEFruiting body formation is traditionally regarded as a defining trait of myxobacteria. Here, we report that Aggregicoccus spp., including six strains of four species, can sporulate but are deficient in the fruiting body-associated genomic information (FAGI). This demonstrates that the non-fruiting characteristic in Aggregicoccus stems from inherent genetic deficiencies rather than suboptimal cultivation. Our findings highlight the need to assess FAGI presence in classifying non-fruiting lineages, innovate the isolation method, and refine our understanding of the diversity and evolution of the myxobacteria.

Aggregicoccus

Metagenomics indicates new taxa in Candidatus Saccharimonadia and proposal of Parviradicicola hetaonensis gen. nov. sp. nov. and Parviputeicola dengkouensis gen. nov. sp. nov. following the rules of the SeqCode.

Candidatus Saccharimonadia is a core lineage within the phylum Patescibacteriota (formerly the bacterial candidate phyla radiation, CPR), yet the class has long lacked a standardized, complete taxonomic framework. This nomenclatural gap severely hinders consistent academic exchange and global research into its diversity, evolutionary history, and ecological roles. Here, we recovered 29 medium- to high-quality Ca. Saccharimonadia metagenome-assembled genomes (MAGs) from groundwater, rhizosphere soil, and saline-alkali soil in the Hetao Irrigation District, Inner Mongolia, China, and performed integrated phylogenomic, genome size evolution, and metabolic analyses alongside reference genomes from the GTDB r220 database. Based on robust polyphasic taxonomic evidence (multi-dimensional phylogenetic analyses, widely accepted genome-wide ANI/AAI thresholds) and SeqCode rules, we formally propose two novel taxa: Parviradicicola hetaonensis gen. nov., sp. nov. (type material: txb011_bin.8.strictTS) and Parviputeicola dengkouensis gen. nov., sp. nov. (type material: sgl022_bin.19.origTS), plus two novel families and one novel order. We further identified potential drivers and important associations related to Ca. Saccharimonadia genome size evolution and adaptive metabolic traits. This work refines the Ca. Saccharimonadia taxonomic framework, providing critical genomic references for follow-up research.

Phylogeny

Description of two novel Marinobacter species isolated from saline-alkali soil: Marinobacter alkalisoli sp. nov. and Marinobacter shunpengi sp. nov.

Four Gram-staining negative, non-motile, rod-shape bacteria, named strains GN3S48T, HN1S83, LN3S78T, and M1N3S26, were isolated from the bulk saline soils, in Baotou, China. Among them, strains GN3S48T and HN1S83 could degrade 100 mg l-1n-hexadecane as sole carbon and energy source for their growth. Phylogenetic analyses showed that the four strains always formed two distinct clades: Strain LN3S78T clustered with strain M1N3S26, and strain GN3S48T clustered with strain HN1S83. Nonetheless, all four strains tightly clustered and shared the highest 16S rRNA gene similarities with Marinobacter species. Specifically, clade of strains LN3S78T and M1N3S26 cluster with Marinobacter lipolyticus CGMCC 1.7282T, while clade of strains GN3S48T and HN1S83 clustered with Marinobacter zhanjiangensis CCTCC AB 208029T. The ANIb and AAI values between strains GN3S48T and HN1S83 were 96.4% and 94.6%, respectively, while those between strains LN3S78T and M1N3S26 were 99.3% and 99.1%, respectively. All ANI and AAI values between the four strains and their closest relatives were below the 95.0% species delineation threshold. The predominant respiratory quinone of the four strains was Q-9. Based on this polyphasic result, the two clades should be identified as two novel species within the genus Marinobacter. Thus, Marinobacter alkalisoli sp. nov. (type strain GN3S48T = CGMCC 1.62232T = KCTC 8701T = JCM 37359 T) and Marinobacter shunpengi sp. nov. (type strain LN3S78T = CGMCC 1.62233T = KCTC 8702T = JCM 37360T) are proposed. The metagenomic analysis revealed that the two new species are globally distributed in high-salt habitats. In addition, comparative genomic analysis confirmed that alkane-degrading genes are ubiquitous in Marinobacter strains.

Marinobacter

Genomic and polyphasic characterization of six novel Hymenobacter species isolated from soil in Korea.

Six bacterial strains (BT523T, BT559T, 15J16-1T3BT, BT730T, DG25AT, and DG25BT) were isolated from soil samples in Korea and assigned to the family Hymenobacteraceae (order Cytophagales, class Cytophagia). Phylogenetic analysis based on 16S rRNA gene sequences showed that the strains formed distinct lineages within the genus Hymenobacter. Strains BT523T, BT559T, and 15J16-1T3BT exhibited highest sequence similarities to Hymenobacter armeniacus BT189T (97.6%), Hymenobacter polaris RP-2-7 T (97.8%), and Hymenobacter paludis KBP-30 T (98.3%), respectively, while strains BT730T, DG25AT, and DG25BT were most closely related to Hymenobacter tibetensis XTM003T, with similarities of 96.3-96.6%. All strains were Gram-negative, aerobic, rod-shaped, and formed red to pink pigmented colonies. Whole-genome analysis revealed genome sizes ranging from 3.78 to 6.33 Mb with G + C contents of 55.5-65.0%. Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between the strains and their closest relatives were below the accepted thresholds for species delineation, supporting their classification as novel species. Functional annotation indicated the presence of genes associated with core metabolism, stress response, and pigment biosynthesis, reflecting adaptation to soil environments. Secondary metabolite analysis further revealed the presence of biosynthetic gene clusters, including terpene and siderophore pathways. Based on polyphasic taxonomic evidence, the six strains are proposed to represent six novel species of the genus Hymenobacter, for which the names Hymenobacter miniatus sp. nov., Hymenobacter madidus sp. nov., Hymenobacter convexus sp. nov., Hymenobacter rubellus sp. nov., Hymenobacter erythromyxa sp. nov., and Hymenobacter radioresistens sp. nov. are proposed. The type strains are BT523T (= KCTC 72341 T = NBRC 114851 T), BT559T (= KACC 21821 T = NBRC 114852 T), 15J16-1T3BT (= KCTC 42995 T = NBRC 112818 T), BT730T (= KACC 22457 T = NBRC 116482 T), DG25AT (= KCTC 32451 T = TBRC 19856 T), and DG25BT (= KCTC 32452 T = JCM 19444 T).

Soil Microbiology