[Infectious ectromelia in virological laboratory--diagnosis and prevention].
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Anterior segment and external ocular manifestations of some infectious diseases which are particularly important to patients of the pediatric age group are discussed. A brief review of the various systemic diseases is given along with the ocular findings. Approaches to diagnosis, therapy, and management of both the systemic and ocular components of the illnesses are included.
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Electron microscopy by negative staining is a well-known, simple and quick diagnostic method to demonstrate virus particles. A method for routine work is described. The morphology of important viruses of the herpes and pox group is shown. In herpes simplex and zoster, the viruses can be detected in most cases from fresh vesicular fluid, whereas older erosions and crusts are not suitable. Viruses have been shown in all cases of chicken pox, vaccinia inoculata, molluscum contagiosum, and ecthyma contagiosum (orf).
Mink (Mustela vison) were inoculated with viruses: African horse sickness (AHS), African swine fever (ASF), bovine herpes virus II (BHV2), foot-and-mouth disease (FMD), goat pox (GP), hog cholera (HC), peste des petits ruminants (PPR), rinderpest (RP), swine vesicular disease (SVD), vesicular exanthema of swine (VES) and vesicular stomatitis (VS). Their susceptibility was measured by development of clinical signs, virus isolation and detection of precipitin and/or virus neutralizing antibodies. SVD virus produced a lesion in one mink. Virus was isolated from mink inoculated with SVD, FMD and BHV2. Neutralizing and/or precipitin antibodies were detected in mink inoculated with ASF, FMD, GP, RP, SVD and VS viruses. Mink were not susceptible to AHS, HC, PPR and VES viruses.
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Data are presented from serological and virological investigations of natural and experimental infections, in rodents, with different poxviruses. The demonstration of poxvirus antibodies (virus neutralizing antibodies, antihaemagglutinins, and precipitins) in the kidneys and/or lungs of rodents from Europe and Africa, and the isolation of poxviruses from them, all indicate that rodents may be poxvirus carriers. Isolates from rodents differed in their properties. Some, from Turkmenistan rodents or from white rats caught near Moscow, appeared to be very close to cowpox virus, while others (from Zaire rodents) were identical to variola-like (whitepox) viruses found earlier in monkeys in the same region. The results suggest that rodents that carry the virus closely similar to cowpox virus might be a source of infection for other animal species.
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Nonhuman primates are extensively used in laboratories as experimental animals. It is necessary, however, to realize that their employment may be dangerous to man and other species of primates, if recognition of their flora and fauna, especially viral, is not considered and if appropriate controls are not followed. Several outbreaks have occurred, resulting in high mortality and morbidity of man and simian. A number of recommendations are provided which, if followed, will minimize the waste of time, money, and life.
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2 pox outbreaks among white rats in a breeding colony are described. The infection occurred in 3 different forms: pulmonary, dermal and mixed. Apparently healthy animals appeared to be virus carriers. The virus isolated belonged to the genus Orthopoxvirus of Poxviridae family, and was very close to cowpox virus. It differed from reference strains of cowpox virus in having a lower ceiling temperature and a higher pathogenicity for white rats.
Particles of a poxvirus found in Californian western grey squirrels have overall dimensions indistinguishable from those of the poxviruses of the genera Orthopoxvirus and Leporipoxvirus, but have surface structures somewhat reminiscent of virions of members of the genus Parapoxvirus. Extracts of tissues infected with the squirrel poxvirus cross-react in gel-diffusion tests with extracts of tissues infected with Californian myxoma virus, but not with a similar preparation infected with a South American strain of myxoma virus.
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