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Results for “Pregnanediol”

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At least 19 recordsLinked to original sources

PubMed1978

The value of urinary pregnanediol estimation for monitoring early pregnancies.

Urine samples from 76 pregnant women were tested for pregnanediol content during the first 6 weeks of pregnancy. Pregnanediol was measured by gas chromatography in 24-hour urine samples obtained once weekly from 76 randomly selected pregnant women 21, 28, 35, and 42 days after the last menstrual period. Pregnancy was ascertained by a positive hemagglutination inhibition test for human chorionic gonadotropin. In patients in whom the urinary pregnanediol content was less than 3 mg/24 hours the abortion rate was 81.5%, and 18.5% had normal pregnancies and births In patients whose pregnanediol content was greater than 3 mg/24 hours the abortion rate was 8.3%, and 91.7% had normal pregnancies and births. The 24-hour urinary pregnanediol excretion rate reflects corpus luteum function and can be considered as a means of monitoring pregnancy in its initial stage.

Chromatography, Gas↗

PubMed1975

Identification and measurement of urinary estrone, estradiol-17 beta, estriol, pregnanediol and androsterone during the menstrual cycle of the orangutan.

Urinary estrone, estradiol-17beta, estriol, pregnanediol and androsterone were identified and measured during 3 menstrual cycles in 2 female orangutans. In 2 of the cycles, the animals excreted 1-8 mug/day estrone, 0.5-6 mug/day estradiol-17beta, 1-8 mug/day estriol, 20-206 mug/day pregnanediol and 120-522 mug/day androsterone during the first half of the menstrual cycle. In the second half of the cycle, corresponding values were 3-21 mug/day estrone, 2-10 mug/day estradiol-17beta, 1-9 mug/day estriol, 54-800 mug/day pregnanediol and 90-1158 mug/day androsterone. In 1 cycle, the estrogen values for the second half were considerably higher, possibly due to the animal becoming pregnant just before this study commenced. The values for estrone and estradiol-17beta are similar to those found in the human and chimpanzee menstrual cycle. The values for estriol were lower than in the human but higher than in the chimpanzee. Levels for urinary pregnanediol and androsterone were significantly lower than in the human. Variations during the menstrual cycle for estrone were characterized by a midcycle peak followed by a second peak in the luteal phase. No definite pattern was apparent for estradiol-17beta or estriol. Both urinary pregnanediol and androsterone levels were low during the first half of the cycle, started to rise just after midcycle, and showed a peak during the second half of the menstrual cycle.

Androsterone↗

PubMed1975

A modification of the Detter and Klingmüller's method for urinary alpha- and beta-pregnanediol and pregnanetriol determination: excretion patterns in men and in women during the normal menstrual cycle.

This report describes the modification of Detter and Klingmüller's method for the determination of urinary alpha- and beta-pregnanediol and pregnanetriol by means of thin -layer chromatography. The modifications were as follows: 1. The addition of formol prior to hydrolysis to prevent pigments penetration into urinary extracts. 2. The use of Kieselgel HF 254+366 nach Stahl (E. Merck, Darmstadt) which allows to localize the spots under UV-light at 366 nm without the use of colour reagents. The results concerning accuracy, sensitivity, precision and specificity in the modification described are profitable. Using this method in 7 healthy women (aged 28-37) with normal menstrual cycles the urinary excretion of alpha- and beta-pregnanediol and pregnanetriol were evaluated every second day (starting the 6th day of the cycle). The maximum of excretion of alpha- and beta-pregnandiol appeared on day 20 of the cycle, with the mean (+/- S.D.) 1.27 +/- 0.37 mg/24hr and 2.97 +/- 0.80 mg/24hr for alpha- and beta-pregnanediol, respectively. Mean values of pregnantriol were at the same level and ranged from 0.25 to 1.42 mg/24 hr. Single determination of these compounds in 8 healthy men (aged 19-42) revealed the mean excretion values (+/- S.D.) 0.96 +/- 0.17 mg/24 hr, 1.24 +/- 0.40 mg/24 hr, 1.12 +/- 0.65 mg/24 hr for alpha- and beta-pregnanediol and pregnanetriol, respectively.

Adult↗

PubMed1977

An improved method for the simultaneous determination of individual 17-oxosteroids and of pregnanediol in urine by gas-liquid chromatography.

An improved method for the simultaneous gas-chromatographic determination of individual 17-oxosteroids and of pregnanediol in the same sample of urine is described. The method involves the following steps. The urinary sample is chromatographed on Amberlite XAD-2. The fraction containing the steroid conjugates is evaporated; the residue is dissolved in n-heptane and the organic phase extracted with water. The steroid glucuronides are hydrolysed by a bacterial beta-glucuronidase; subsequently, the steroid sulphates are solvolysed in ethyl acetate. Free 17-oxosteroids and pregnanediol are separated by two-dimensional thin-layer chromatography. The zone containing the steroids to be investigated is eluted; after evaporation, the residue is treat with N-methyl N-trimethyl silyl trifluoroacetamide. The reaction mixture is injected into a gas chromatograph; the trimethyl silyl ethers of the individual 17-oxosteroids and of pregnanediol are quantitated by measuring the peak heights.

17-Ketosteroids↗

PubMed1977

Analysis of pregnanediol in urine: trental interference.

Patients given Trental (pentoxifyllin, Hoechst A.G.) for the treatment of vascular disorders excrete a substance which interferes with the gas chromatographic anlaysis of pregnanediol. The interfering substance appeared in urine within 4-5 h of the ingestion of 100 mg Trental, and was cleared during the next 4-10 h. The administration of 300 mg/24 h to an anovulatory woman caused an apparent increase in her output of pregnanediol of from less than 1 to 65.8 micronmol/24 h.

Chromatography, Gas↗

PubMed1978

[Studies on the measurements of urinary pregnanediol by radioimmunoassay (author's transl)].

A simple and practical radioimmunoassy (RIA) for pregnanediol without any pretreatment of urine specimens such as hydrolysis and/or extraction was developed. Antisera were produced by immunizing rabbits with pregnanediol-3-glucuronide (P-diol-3-G)-BSA conjugates and P-diol-3-hemisuccinate-BSA conjugates. The specificities of the antisera were examined by their cross-reactivities with related steroids. Antiserum to P-diol-3-G-BSA was significantly cross-reacted with 20 alpha-OH-P(201%), P-diol(100%), P -diol-3-G (77.3%, where 119% as free P-diol), to a lesser extent with 20 beta-OH-P(17.8%), and did not react with other steroids. Antiserum to P-diol-3-hemisuccinate-BSA, on the other hand, was capable of reacting with P-diol(100%), P-diol-3-G(46.9%), 20 alpha-OH-P(75.2%) and 20beta-OH-P (8.97%), respectively. Antiserum generated against P-diol-3-G-BSA was almost twice as high in its avidity for P-diol-3-G as compared with antiserum raised against P-diol-3-hemisuccinate-BSA. Antiserum to 20alpha-OH-P-3-carboxy-methyl oxime-BSA conjugate was only reactive with 20alpha-OH-P and not with P-diol or P-diol-3-G. From the data presented here, antiserum to P-diol-3-G-BSA with high specificity toward urinary P-diol-3-G was used for the present assays. Ammonium sulfate precipitation was employed for the separation of antibody-bound and free tracer hormones. A direct method in which simply diluted urine was directly applied to RIA and the enzymatic hydrolysis method which requires extraction of P-diol with ether following hydrolysis of urine by beta-glucuronidase were both examined in the present RIA on the same specimens which contained known amounts of 3H-P-diol-3-G. Values obtained by the direct method were in good correlation with those determined by the hydrolysis method (r=0.994 p less than 0.01, Y=1.07X + 0.30). The working range of the assay was from 50pg to 5ng of P-diol per tube, and intra- and interassay precisions were 9.8, 12.5 and 12.9, 16.7%, respectively. Determination of radioimmunoassayable P-diol-3-G in urine was also in good correlation with the values obtained by the currently used analytical procedure, the Klopper-Kambegawa method.

Animals↗

PubMed1979

The concentrations of urinary oestrone-3-glucuronide, LH and pregnanediol-3alpha-glucuronide as indices of ovarian function.

Methods based upon the principles of radioimmunoassay have been developed and evaluated for the measurement of oestrone-3-glucuronide, LH and pregnanediol-3alpha-glucuronide in samples of unextracted urine. The procedures have been applied to daily urine (early morning fraction and combined 24 hour collections) from 6 women throughout one complete menstrual cycle and to serial samples from an additional 14 women who only collected early morning specimens. The results showed that there were characteristic, well-defined changes in the concentration of all 3 metabolites in both samples of urine and from all subjects. In addition, there was a reasonable correlation between the concentration of all 3 compounds in samples of early morning urine and the corresponding rates of excretion per 24 hours. These findings suggest that the procedures may be of value for monitoring ovarian function over long periods of time, without the problems of stress and inconvenience to the patient. Furthermore, the ratio of values for oestrone-3-glucuronide to pregnanediol-3alpha-glucuronide may be used to indicate the start and finish of the fertile period.

Adult↗

PubMed1979

[Pregnanediol and pregnanetriol levels in the urine of rats after thymectomy].

The authors examined arterial blood pressure and excretion of pregnanetriol and pregnanediol in urine of adult male rats of Westar strain under normal conditions and after thymectomy. There was an elevation of arterial blood pressure after 45th day of thymectomy. Excretion of pregnanediol was increased on the 30th day, but that of pregnanetriol on the 60th day after removal of the gland. The increased excretion of both progesterone metabolites was connected with increased renal function, which probably played pathogenic role in postthymectomized hypertensive reaction.

Animals↗

PubMed1978

The measurement of steroid glucuronides in urine from mice to monitor gonadal function. I. Pregnanediol-3 alpha-glucuronide as an index of progestogen output.

An attempt has been made to assess the output of progesterone in mice by measuring the concentration of pregnanediol-3 alpha-glucuronide (PdGl in serial samples of unextracted urine. Longitudinal studies with 95 animals have shown that sufficient urine (greater than 40 microliter) can be obtained from individual animals (80% success rate) without stress every day or at intervals of 2 h. The sc administration of 50 microgram progesterone in lauric acid ethyl ester resulted in a 9-fold increase in the concentration of urinary PdGl (within 4 h) and a significantly elevated output for the next 26 h. During the induction of superovulation with PMS and hCG, the values of PdGl increased significantly (P less than 0.0005; by Student's t test) from 2.08 +/- 0.97 to 3.99 +/- 2.55 ng/ml (mean +/- SD) at the time of corpus luteum formation. There was a good correlation (r = 0.84; n = 154) between the values expressed as nanograms per ml urine and nanograms per mg creatinine. The results demonstrate that the concentration of PdGl in serial samples of urine may be used as an index of gonadal function in female mice.

Animals↗

PubMed1978

[A chromatographic method for the urinary pregnanediol dosage for routine analyses (author's transl)].

The AA. aiming at a handy and speedy system admitting of being fitted to the equipments of any laboratory hospital have taken into account two procedures, one proposed by Varley and the other of Fotherby and Love reported by Wootton and King for pregnanediol. The two methods have been combined in order to make the process easier. The procedure is based upon chromatography on alumina of ethereal extracts dissolved in cyclohexan. Glucuronate hydrolysis has been obtained with beta-glucuronidase.

Chromatography, Gel↗

PubMed1977