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At least 19 recordsLinked to original sources

PubMed1979

[Organization of the connections of the putamen with the cerebral cortex and hypothalamus].

Connection between the putamen, the brain cortex and the hypothalamus, as well as the role of the former in different aspects of purposive behaviour have been studied in a complex morpho-physiological investigation. In 12 cats, after developing a symmetrical active-defensive conditioned reflex, unilateral electrolysis of the putamen has been performed and the number of conditioned-reflexive reactions have been counted before and after coagulation. The brains have been treated after Nauta--Gygax, Fink--Heimer with additional staining after Kawamura--Niimi. Monosynaptic connections of the putamen with frontal, precentral, postcentral, orbital, parietal cortical areas have been revealed; direct pathways from the putamen to the infundibulum of the grey tuber, to the posterior and lateral hypothalamic nucleus have been demonstrated; participation of the putamen in the formation of active-defensive conditioned reflexes has been stated, as well as in emotional behaviour with a preferable use either the right or the left foreleg.

Animals↗

PubMed1992

Coactivation of dopamine D1 and D2 receptors increases the affinity of cholecystokinin-8 receptors in membranes from post-mortem human caudate-putamen.

The effects of dopamine in vitro were investigated on the binding sites for cholecystokinin-8 (sulphated, CCK-8) and neurotensin in membrane preparations of the caudate-putamen and nucleus accumbens of post-mortem human brains. Dopamine reduced the IC50 value of competition curves with CCK-8 for [125I]CCK-8 binding in membranes from the caudate-putamen, but not the nucleus accumbens, with a maximal decrease of -25 +/- 9% at 300 nM of dopamine. This decrease could be antagonized by 100 nM of SCH 23390 or 100 nM of raclopride. Kinetic analysis of [125I]CCK-8 binding showed a decrease in the first order dissociation rate constant and in the kinetic Kd (-22 +/- 6% and -24 +/- 6%, respectively) at 300 nM of dopamine, without any significant effect on the apparent or actual association rate constant. Competition curves with neurotensin versus [125I]neurotensin were not affected by dopamine (10-1000 nM) in membranes from the caudate-putamen or the nucleus accumbens. These results suggest that dopamine, by synergistic stimulation of both D1 and D2 receptors, selectively increases the affinity of CCK-8 receptors in the human caudate-putamen, by a selective inhibition of ligand dissociation. This increase may reflect a positive feed-back mechanism, further enhancing the modulatory effects of CCK-8 on dopamine neurotransmission.

Adult↗

PubMed1978

[Connections between the globus pallidus and putamen and the hypothalamus and subthalamus].

In 16 adult cats with electrolytically destructed external and internal parts of the globus pallidus and in 8 cats with destructed putamen direct strio-pallido-hypothalamic and strio-pallido-subthalamic pathways have been studied. Degeneration of the axonal preterminals and terminals have been examined in preparations treated after Nauta--Gygax, Nauta--Laidlow, Finck--Heimer with simultaneous additional staining of the nuclei with cresyl violet after Kawamura--Niimi. Direct pallido- and putamen-hypothalamic pathways to nuclei of the grey tubercle, posterior and lateral nuclei of the hypothalamus were stated. Direct pathways from the putamen to the subthalamic nucleus have been revealed, however, these pathways are represented in less degree than those of pallido-subthalamic connections. Direct pathways from the external portion of the globus pallidus and putamen to the subthalamic nucleus are more pronounced and represented by greater numbers of projections than those of strio-pallido-hypothalamic origin.

Animals↗

PubMed1978

[Connections between the globus pallidus and putamen and the cerebral cortex].

The results are represented on investigation of the projections of the globus pallidus and putamen on the brain cortical fields. In 17 cats a unilateral electrolytic destruction of the exterior and interior globus pallidus, and in 8 cats--unilateral electrolysis of the putamen were performed. The brains were treated after Nauta--Gygax, Fink--Heimer, Kawamura--Niimi. Direct connections between the external globus pallidus with prepiriformic, orbital (field 43), temporal (fields 20, 22), frontal (field 6), precentral (field 4), postcentral (field 2) cortical areas were revealed, and projections of the putamen on the frontal (field 6), precentral (field 4), postcentral (field 2), orbital (field 43) cortical areas were demonstrated. Less fibres connect the putamen with temporal and parietal areas. No convincing evidence was obtained to demonstrate direct connections of the entopedunctular nucleus with the brain cortex.

Animals↗

PubMed1977

[Projections of different areas of the putamen on tha thalamic nuclei].

In the work presented the projections of rostral and caudal areas of the putamen on the thalamic nuclei were studied. The analysis of the frontal serial sections from the cat brains impregnated according to the methods of Nauta-Gygax, Finck-Haimer, Wiitanen made it possible to state that all the areas of the putamen radiate a great number of axons to the lateral thalamic nuclei--ventro-anterior, ventro-lateral, lateral-posterior, and ventro-posterior-medial. Only the rostral area of the putamen sends a mass of fibres to the nuclei of the median thalamus--medial-dorsal, central, central-lateral and ventro-posterior-lateral. On the other hand, in the posterior thalamic nucleus a great number of axons only from caudal area of the putamen have their terminals.

Animals↗

PubMed1977

Degeneration of two of nine types of synapses in the putamen after center median coagulation in the cat.

The cat putamen contains the identical nine types of synapses and the same proportion of axo-dendritic (or axo-somatic) synapses as described for the fundus striati. However, type III (cortico-striatal) (31:16%) and type V Caxon-callateral) (13:1%) occur much more frequently and type I (nigro-striatal) much less frequently (14:34%) in the putamen than in the fundus striati. Of the axo-spinous synapses only type IV, with densely arranged small round vesicles and interrrupted, asymmetric contact, shows a dark degeneration after center median lesions, mainly in the parvocellular part. Of the six axo-dendritic (or axo-somatic) synapses, only type VII, with densely packed small round vesicles and asymmmetric contact, is degenerated after the same lesion in the center median nucleus. However, after such lesions type VII synapses are much more frequently degenerated in the putamen than those of type IV.

Animals↗

PubMed1976

Postnatal development of acetylcholinesterase in the caudate-putamen nucleus and substantia nigra of rats.

The postnatal development of acetylcholinesterase (AChE, EC 3.1.1.7) and NADH-diaphorase was examined in the caudate-putamen nucleus and substantia nigra of rats ranging from 3 to 90 days in age. From 3 to 15 days post partum islands of AChE and NADH-diaphorase activity were observed in the caudate-putamen nucleus. Individual neuronal somata could also be seen in AChE-stained sections up to 15 days. At later ages neuropil staining became increasingly dense, and this presumably accounted for the infrequent visualization of cell bodies in the brains of older animals. During development AChE appeared in the caudate-putamen nucleus in a lateral to medial topographic order; analogously, enzyme staining in the neostriatum reappeared in the same lateral to medial topographic order in adult rats following irreversible AChE inhibition by intramuscularly injected bis-(1-methylethyl)phosphorofluoridate (di-isopropylfluorophosphate: DFP). Furthermore, DFP treatment in mature animals revealed the presence of AChE in striatal neurons having morphologies similar to those observed in newborn rats. A similar time-course of postnatal AChE development was observed in the substantia nigra. In both the pars compacta and pars reticulata individual cell bodies, which were visible at early ages (3-10 days), became increasingly obscured at later times after birth by extra-somata staining. Between the 6th and 15th postnatal days AChE-containing fibers were seen projecting apparently from pars compacta into pars reticulata. Comparison of the present results with histochemical data of other investigators on the postnatal development of monoamines indicated the likelihood of cholinergicmonoaminergic interactions in the neostriatum and substantia nigra.

Acetylcholinesterase↗

PubMed1979

Autoradiographic localization of neuroleptic and dopamine receptors in the caudate-putamen and substantia nigra: effects of lesions.

The localization of neuroleptic receptors was studied in the caudate-putamen (CP) and the zona compacta of the substantia nigra using light microscopic autoradiography of 3H-spiperone binding sites. Lesion of the dopaminergic input to the caudate-putamen produced an increase in receptors in the CP, possibly reflecting denervation supersensitivity. Kainic acid lesions and decortication produced significant decreases of 61% and 18% in striatal receptors. This suggests that in the caudate-putamen most of the dopamine receptors are on intrastriatal neurons, but some are also localized to the afferents from the cortex. Lesion of the nigro-striatal dopaminergic pathway produced a large (48%) decrease in receptor sites in the substantia nigra zona compacta while kainic acid intrastrially and striato-nigral pathway lesions had no significant effect. These results suggest that the majority of dopamine receptors in the zona compacta which bind neuroleptics are located on cell bodies and processes of dopaminergic neurons and are anatomically distinct from dopamine-stimulated adenylate cyclase sites.

Animals↗

PubMed1992

Combined microdialysis and Fos immunohistochemistry for the estimation of dopamine neurotransmission in the rat caudate-putamen.

Extracellular dopamine (DA) concentrations estimated by transcerebral dialysis and D1-dependent c-fos expression, as demonstrated by Fos immunohistochemistry, were studied after blockade of DA reuptake by GBR-12909. Rats implanted with dialysis probes in the dorsal caudate-putamen did not show any Fos-positive neuronal labeling in the implanted area or in the rest of the caudate-putamen. Administration of GBR-12909 dose-dependently increased DA output in dialysates and resulted in the appearance in the caudate-putamen of Fos-positive neurons whose density was related to the dose of GBR-12909 and to the increase in extracellular concentrations of DA. The D1 antagonist SCH-23390 blocked GBR-12909-induced activation of Fos while potentiating the stimulation of DA output. The results show that following blockade of DA reuptake by GBR-12909, the induction of Fos is related to stimulation of D1 receptors by extracellular DA. Combination of brain dialysis with Fos immunohistochemistry might provide a method for estimating the functional significance of extracellular DA as measured by brain microdialysis.

Animals↗

PubMed1976

Dopamine evoked inhibition of single cells of the feline putamen and basolateral amygdala.

1. In cats under pentobarbitone or halothane anaesthesia, neurones of the putamen and basolateral amygdala were inhibited with a similar time course by iontophoretic applications of dopamine and gamma-aminobutyric acid (GABA), ejected with relatively short (20 sec) low intensity (less than 40 nA) pulses of positive current from five and seven barrelled extracellular micropipettes. The use of a stereotaxically positioned guide tube, sealed to the skull with dental cement, made it possible to obtain stable recording conditions and to correlate the stereotaxic position of the cells with the position of the micro-electrode tracks determined histologically by the post-mortem reconstruction of serial sections. 2. Since in cats anaesthetized with pentobarbitone none of the cells were found to be spontaneously active, the relative potency of dopamine and GABA were compared on glutamate excited cells. Approximately 2-5 times more current was required to release sufficient dopamine to cause just submaximal inhibition, equal in magnitude and duration to that evoked by GABA. 3. In nitrous oxide/halothane anaesthetized cats, approximately one quarter of the cells were spontaneously active. Relative potency studies showed that for dopamine, currents 2-0 and 1-6 times larger than those used for GABA were required to inhibit glutamate excited and spontaneously active cells respectively. 4. When the depth distribution of the cells was compared with the sensitivity of the cells to dopamine and GABA, the most sensitive cells were found to lie within the putamen and the basolateral amygdala. 5. On more than one third of the cells tested, iontophoretic application of the neuroleptic, alpha-flupenthixol of more than 3 or 4 min in duration, greatly reduced or abolished the inhibition of the cells by dopamine without impairing their sensitivity to GABA. 6. In four cats, large I.V. injections of alpha-flupenthixol (10 mg/kg) and the more potent neuroleptic pimozide (1 mg/kg) had no significant effect on the dopamine or GABA sensitivity of seventy cells in the putamen and basolateral amygdala. 7. Our results are in keeping with the view that dopamine has a predominantly inhibitory action in the mammalian forebrain. However the failure of I.V. neuroleptics to modify the sensitivity of the cells to dopamine suggests that the dramatic effects of neuroleptics on animal behaviour may not be explicable simply in terms of a generalized blockade of dopamine receptors at post-synaptic sites.

Action Potentials↗

PubMed1992

[Effect of injection of enkephalin and bestatin in caudate-putamen on operant conditioning in rats].

Female Wistar rats were trained in a Skinner-box, 30 trials per day in a dark room to establish operant defence conditioning. Training started with a light (15 s), then combined with footshock for further 8 s. When the rats learned to press the key to avoid footshock within 15 s, conditioned response was considered established. After the rats reached a conditioning rate (CR) above 80% for 5 days, cannulae were implanted into caudate-putamen. Two to three days later, Met-enkephalin (MEK) or bestatin (an aminopeptidase inhibitor) was injected bilaterally into caudate-putamen. 30 min, 2 h, 24 h and 48 h after injection, conditioning tests were conducted, with each session consisting of 30 trials. Control experiments were done when 0.9% NaCl (NS) was injected. After injection of NS, CR maintained above 80% in all 4 test sessions. MEK (60 ng/rat) or bestatin (10 micrograms/rat) significantly lowered the CR during the 30 min and 2 h test session. In the latter case, the latency (L) was also prolonged. However both CR and L returned to the control level in the 24 h and 48 h test sessions. Naloxone (2 mg/kg, i.p.) blocked the conditioning-depression effect of bestatin. No significant alteration was seen in locomotor activity after MEK or bestatin injection. The results suggest that enkephalin in caudate-putamen may be involved in the regulation of retrieval of conditioning. Bestatin mimics the effect of MEK on conditioning reflex probably by increasing production of endogenous enkephalin.

Animals↗

PubMed1976

[Biometrical fresh volume analysis of nucleus caudatus, putamen and globus pallidus in an ontogenic series of Tupaia belangeri].

The fresh volumes of the nucl. caudatus (including the nucl. accumbens septi), putamen, globus pallidus and of the whole brain of 37 male Tupaia belangeri aged between 36-536 days of ontogenesis have been determined. The growth of these nuclear regions is described by the logistic function. The parameters were approximated by means of an iterative procedure by the least square method. The "ideal" volume of the globus pallidus is 9,6 mm3, of the striatum 113 mm3, and of the whole brain 3 260 mm3. The degrees of maturity of globus pallidus, striatum and the whole brain do not differ significantly from each other concerning the date of birth (43th day of ontogenesis), the growth factors are about 5,5. The nucl. caudatus, including the nucl. accumbens septi, and the putamen demonstrate almost the same course in their degrees of maturity and growth dynamics within the tested age interval. In the course of postnatal development the globus pallidus grows more quickly than the whole brain, and the whole brain, on the other hand, more quickly than the striatum. The half value time of the globus pallidus is about 53 days of ontogenesis, of the whole brain about 56 days of ontogenesis and of the striatum about 58 days of ontogenesis. On the 70th day of ontogenesis, the difference between the degrees of maturity of globus pallidus (93 per cent) and striatum (= nucl. caudatus + nucl. accumbens septi + putamen) (72 per cent) is 21 percent. This complicated growth dynamics can be seen exactly by considering the non-linear logistic functions, the degrees of maturity, and the growth-rate of the degrees of maturity.

Age Factors↗

PubMed1992

Cellular basis for interactions between catecholaminergic afferents and neurons containing Leu-enkephalin-like immunoreactivity in rat caudate-putamen nuclei.

Dopaminergic afferents to the dorsal striatum, caudate-putamen nuclei, are known to modulate the levels and synthesis of endogenous opiate peptides (Leu5 and Met5-enkephalins). We examined the dual immunocytochemical localization of antisera raised against Leu5-enkephalin and the catecholamine-synthesizing enzyme, tyrosine hydroxylase (TH), to determine the cellular substrates for these and/or other functional interactions. The antisera were identified by combined immunogold-silver and immunoperoxidase labeling in single coronal sections through the caudate-putamen nuclei of adult rats. These animals were given intraventricular injections of colchicine, and the brains were fixed by acrolein perfusion prior to immunocytochemical labeling. By light microscopy, perikarya and processes containing enkephalin-like immunoreactivity (ELI) were seen in close proximity to varicose processes immunoreactive for TH. Electron microscopy further demonstrated that the ELI was localized to perikarya, dendrites, and axon terminals, whereas the TH was exclusively in axons and terminals. The dendrites containing ELI were postsynaptic to terminals that were either (1) without detectable immunoreactivity, or (2) immunoreactive for TH or enkephalin. Nonsynaptic portions of the dendrites containing ELI were covered with astrocytic processes or were in direct apposition to unlabeled dendrites. Terminals containing ELI were densely immunoreactive and were in direct contact with (1) unlabeled and occasionally enkephalin-labeled proximal dendrites, and (2) TH-labeled and unlabeled terminals. In comparison with the opiate terminals, most catecholaminergic terminals were lightly immunoreactive for TH and usually contacted more distal unlabeled dendrites or spines and, more rarely, dendrites containing ELI. In a few favorable planes of section, the terminals containing ELI and those containing TH (1) converged on common unlabeled dendrites, or (2) formed dual contacts on two different labeled or unlabeled targets. Junctions formed by terminals containing ELI and TH were sometimes characterized by symmetric synaptic densities. However, numerous other dendritic and all axonal appositions were without recognized membrane densities. The findings of the study provide anatomical substrates for multilevel interactions between catecholamines, mostly dopamine, and enkephalin in rat dorsal striatum. These include (1) monosynaptic input from dopaminergic terminals to neurons containing enkephalin, (2) presynaptic modulation of transmitter release through axonal appositions, and (3) dual regulation of common targets through convergent input. In addition, the findings suggest that both enkephalin and dopamine may have similar modulatory roles in synchronizing the activity of dual targets postsynaptic to individual axon terminals. Alterations in any one of these multiple types of interactions could account for noted motor or sensory symptoms in neurological disorders characterized by depletion of dopamine or endogenous opiate peptides, or both.

Animals↗

PubMed1976

Dopaminergic substrates of intracranial self-stimulation in the caudate-putamen.

An extensive mapping of the caudate-putamen in rat for intracranial self-stimulation (ICS) site was undertaken to provide addtional support for the role of dopamine in brain-stimulation reward. Eight-seven per cent of the placements in the neostriatum supported ICS, with self-stimulation rates greater than 250/15 min at 56% of these sites. Electrical stimulation also elicited rearing and clonus, and contralateral body turn, both of which varied in magnitude between animals. In a second experiment, animals were prepared with electrodes aimed at the lateral caudateputamen. Those subjects displaying ICS subsequently received 6-hydroxydopamine lesions to the dopamine cell bodies in the substantia nigra pars compacta, either ipsilateral or contralateral to the electrode. The destruction of the dopamine cell bodies attenuated ICS in both groups during the first post-lesion test sessions. However, the rates in the ipsilateral group declined to between 2 and 9% of control scores, whereas the rate in the contralateral group improved over testing to 72% of control values, 28 days after the lesion. On the basis of these data, it was concluded that unilateral destruction of the dopaminergic nigro-neostriatal bundle (NSB) has two effects on ICS behavior. First, unilateral reduction of neostriatal dopamine is accompanied by a loss of brain-stimulation reward at sites normally innervated by the NSB, specifically the caudate-putamen. Secondly, lesions of the NSB produce a general disruption in bar-pressing behavior, as evidenced by the attenuation of ICS following contralateral lesions.

Animals↗

PubMed1992

Chronic neuroleptic administration decreases extracellular GABA in the nucleus accumbens but not in the caudate-putamen of rats.

The extracellular levels of gamma-aminobutyric acid (GABA) in the caudate-putamen and the nucleus accumbens of rats following administration of haloperidol decanoate, fluphenazine decanoate, or vehicle for 8 months were assessed using intracranial microdialysis. Basal levels of extracellular GABA were significantly decreased in the nucleus accumbens of both neuroleptic-treated groups while levels of GABA in the caudate-putamen were not significantly different between groups. These results provide evidence for selective chronic neuroleptic-induced effects on in vivo GABA function in different terminal regions containing dopamine receptors.

Animals↗

PubMed1978

Nerve cell loss with aging in the putamen.

A significant correlation between age and decrease in the number of nerve cells has been found in the putamen. Both small and large cells in this nucleus were proportionally involved. A possible relationship with the outfall of cells in other structures of the brain, with which the putamen is connected, in order to balance the activity of the extrapyramidal system has been suggested.

Adolescent↗

PubMed1976

[Efferent projections of the ventral portion of the putamen to the frontal, parietal and temporal regions of the cat cerebral cortex].

Following the electrolytic lesion of putamen (rostral and caudal parts of the ventral field) of the cat's brain, degenerating endings of axons in the cerebral cortex were revealed ipsi and heterolaterally in frontal sections of the brain impregnated after Nauta--Gygax and Wiitanen. The projections were organized topically: the rostal part of putamen was projected to anterior parts of the cortex (motor and premotor, orbito-insular and S zones), while the caudal part sended the fibres to the occipital and auditory fields of the cortex and S2 zone.

Animals↗

PubMed1976

Differential effects of para-chlorophenylalanine on self-stimulation in caudate-putamen and lateral hypothalamus.

Rats were prepared with chronic bipolar electrodes aimed at either the caudate-putamen or lateral hypothalamus and those displaying consistent self-stimulation were given additional training at half-maximal current intensities. All subjects received an intragastric injection of para-chlorophenylalanine (400 mg/kg) and self-stimulation tests continued until pre-injection rates were re-established. Responding in both brain areas was suppressed 24 h after drug treatment. The next day, self-stimulation rates in the hypothalamus increased to 115% of pre-drug levels reaching a level of 180% by the third day of post-drug testing. In contrast, self-stimulation of sites in the neostriatum continued to decline, with minimal levels reaching 48% of control on the fourth post-drug day. Self-stimulation rates in both groups had returned to control levels by post-drug day 6. These data indicate that the role of serotonergic mechanisms in brain stimulation is locus specific, and that the specific nature of this role may be determined by interaction with other neurochemical systems. The possible interaction between dopaminergic and serotinergic mechanisms in the neostriatum is discussed as a model of self-stimulation in this region of the brain.

Animals↗