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At least 19 recordsLinked to original sources
PubMed1989

[Changes induced by sarcolysine in vivo in the composition of DNA-bound lipids in sarcoma 37].

The two DNA fractions were isolated from sarcoma 37 by the use of the phenol method: supramolecular complex of DNA (SC DNA, 60%) and "phenol" nuclear matrix DNA (PNM DNA, 40%). The lipids in SC DNA represented of light and tightly bound components, the latter was similar to the lipid composition of PNM DNA. SC DNA contains 20 micrograms of neutral lipids (NL) and 6.5 micrograms of phospholipids (PL), while PNM DNA contains 9.8 micrograms of NL and 3.5 micrograms of PL per mg DNA. SC DNA-bound lipids of sarcoma 37 are deficient in free cholesterol (FC, 13%), but rich in cholesterol esters (CE, 39%) and free fatty acids (FFA, 23%); very rich in cardiolipin (CL, 43%) and phosphatidylethanolamine (PE, 28%), but deficient in phosphatidylcholine (PC, 12%). The tumor contains triglycerides (TG) that is absent in DNA of the normal cells. The injection of sarcolysine (10 micrograms/kg) markedly increased (1.5-3 times) the content of all LN and PL fractions in SC DNA, which was accompanied by both the accumulation of FC, TG, PC and the reduction of the remaining lipid fractions in PNM DNA. It is supposed, that DNA-bound lipids may be the target for the action of sarcolysine.

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PubMed1976

In vivo synchronization of murine sarcoma 37 ascites tumor cells by use of hydroxyurea.

Synchrony of DNA synthesis in mouse sarcoma 37 ascites cells was achieved in vivo by injection of 0.75 mg hydroxyurea per gram weight of mouse. DNA synthesis was gauged by comparing 3H-labeled thymidine incorporation, under defined conditions, to total DNA measured colorimetrically. DNA synthesis in the sarcoma 37 cells fell to negligible levels 4 hours after injection and remained at or below levels observed in randomly dividing cells up to 18 hours after injection. A sharp increase in DNA synthesis occurred at 20 hours, followed by a decline again at 22 hours after hydroxyurea injection. DNA synthesis in the intestine remained below control values at 20 hours.

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PubMed1957

The fine structure of the cells in mouse sarcoma 37 ascitic fluids.

The tumour cells and the reaction cells in Sarcoma 37 ascitic fluids have been studied in thin sections with the electron microscope. The reaction cells were either leucocytes or much larger acidophilic peritoneal cells of the same dimensions as the tumour cells; the peritoneal cells formed as much as 20 per cent of the large cell population. The fine structure of the cells is described and some new observations recorded. It has been found that the cell membrane of eosinophil granulocytes has a laminated composition and the characteristic granules of these cells a double limiting membrane. The pores in the double nuclear membrane of the peritoneal cells have been observed to have a fine line running across them. In the tumour cells, a rounded granular body with a central dense area has been found in the region of the centrosome; these cells were also seen to contain rows of parallel smooth surfaced cisternae lying 150 mmicro apart similar to those hitherto only observed in spermatids. There was a feltwork of fine filaments in the cytoplasm of the centrosome region of the tumour cells. The cytoplasmic fine structure underlying the basophilia of the tumour cells and the acidophilia of the peritoneal cells is compared and discussed.

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PubMed1963

The use of tetranitro-blue tetrazolium for the cytochemical localization of succinic dehydrogenase. Cytochemical and cytological studies of sarcoma 37 ascites tumor cells.

Succinic dehydrogenase (SDH) was localized in the mitochondria of Sarcoma 37 ascites tumor cells by the use of tetranitro-BT (TNBT) and nitro-BT (NBT) in smear preparations. Results with each tetrazolium salt as electron acceptor were evaluated with respect to: (a) size and shape of the formazan precipitate relative to standard mitochondrial morphology; (b) crystallization phenomena of reduced dye; (c) lipid adsorption of formazan. The association of formazan- or iron hematoxylin-stained mitochondria with lipid droplets within the cells was investigated, as was also the influence of formalin fixation, with and without cold acetone pretreatment, on mitochondrial morphology and enzymatic staining. Data from these studies appear to indicate that TNBT is more suitable than NBT for use as a cytochemical reagent in oxidative and/or dehydrogenase enzyme histochemistry and cytochemistry.

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PubMedDate not supplied

[Effect of the plan of using antitumor preparations on their efficacy in sarcoma 37].

In tests staged on animals with sarcome 37 the effect of sarcolysine, cyclophosphamide, alexan and cytozine-arabinozine was studied with different scemes of their application. It was found that the degree to which the drugs inhibit the growth of sarcoma 37 depends on the scheme adopted for their administration. In the case of sarcolyzine the highest effect was achieved with a 5-day introduction of the drug (93 per cent of inhibition and resorption of some tumours); cyclophosphamide proved effective with its single administration or an introduction with an interval of 96 hours (78 per cent of inhibition), alexan was active with a 5- and 10-day courses (78 per cent of inhibition and resorption of some tumours). Sarcoma 37 is shown to be a suitable model for studying the activity of drugs depending upon the scheme of their application.

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PubMedDate not supplied

[Repair of the nucleoid of sarcoma 37 cells in vitro or in vivo. The effect of HEPES buffer].

The method of sedimentation was used to study the repair of Sarcoma 37 cell nucleoid after irradiation of cells in vitro or after irradiation of mice with a dose of 9 Gy. It was shown that the process of repair in vitro was completed after 10 min of incubation of cells in the nutrient medium while the repair in vivo ceased 120 min after irradiation. In the presence of HEPES buffer (20 mM) in the medium the kinetics of the nucleoid repair in cells incubated in vitro was the same as that in vivo.

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PubMed1984

[Effect of combined exposure to an interferon inducer, irradiation and 5-fluorouracil on sarcoma 37 in mice].

Oral administration of a liposome-encapsulated agent triggering the synthesis of interferon--poly(I) poly (C)--to mice bearing solid sarcoma 37 was followed by (a) production of an interferon which circulates for a longer period after preliminary administration of "empty" liposomes; (b) lengthening of the period of tumor latency; in some cases tumor failed to appear at all; (c) higher efficacy of radiation treatment which was manifested by a higher inhibition of tumor growth or complete regression; (d) increased antitumor effect of 5-fluorouracil, and (e) suppression of proliferative activity of tumor.

Animals↗
PubMed1976

[Synchronization of cellular proliferation in mice with sarcoma 37 by using hydroxyurea].

The synchronizing effect of hydroxyurea on the passage of sarcoma 37 cells through the stage of the S-phase and mitosis was investigated in albino mice. The study was performed with consideration to the circadian variations in the mitotic activity and the labelled nuclei indices. The degree of synchronization was assessed by the changes in the cell count and the rate of changes in synchronicity. The tumour consisted of at least two cell populations in which the variations in the number of cells both at the S-period, and, possibly, in mitosis were shifted in respect to one another by phase. The rate of artificial cell synchronization in the mitosis proved to be much greater than the natural one in the tumour undivided into individual populations. However, the number of cells subjected to artificial synchronization showed no significant difference not only from the cell count in the tumour undivided into individual populations, but also from the number of cells synchronized naturally in one of the populations. This could be explained by the fact that hydroxyurea acted on one group of cells only since variations in the number of DNA-synthesizing cells were shifted in individual populations in respect to one another by phase.

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PubMed1978

Testing of 2,3-dihydro-1,3-6H-oxazine-dione (3-oxauracil) in mouse L1210 leukemia and 37 sarcoma.

The antimetabolite 2,3-dihydro-1,3-6H-oxazine-dione (3-oxauracil) has been investigated in an attempt to elucidate its effects on survival time of DBA/2 and B6D2F1 mice with L1210 leukemia and of outbred albino mice with 37 sarcoma. A significant increase in survival time was observed in mice with 37 sarcoma and slight effects were observed in mice with L1210 leukemia when treated with 3 x 12.5 or 3 x 25 mg/kg of 3-oxauracil. No toxic side effects were observed when large therapeutic doses of the drug were given.

Animals↗
PubMed1967

Studies on cell deformability. 3. Some effects of EDTA on sarcoma 37 cells.

The nonlethal procedure of incubation in EDTA solution makes the peripheral regions of ascites sarcoma 37 cells more easily deformable, as reflected in measurements of the decreased amount of negative pressure required to suck out standard hemispherical bulges from the cells into micropipettes. The facilitation of deformability was abolished after reincubation of cells in calcium-containing saline, and this mechanical parameter was partially restored to normal after reincubation in magnesium-containing saline; the mechanical effect of EDTA treatment is, therefore, thought to be due mainly to the removal of calcium from the cell periphery. As EDTA treatment produces no detectable change in cellular electrophoretic mobility, it is concluded that peripheral calcium must be bound to anionic sites deeper than about 10 A from the cellular hydrodynamic slip plane. The data are discussed with emphasis on the view that they should not be extrapolated freely to other cell types.

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