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At least 19 recordsLinked to original sources
PubMed1957

Agglutinating efficiency and combining capacity of Shigella and Vibrio antisera from rabbits at different stages of immunization.

Studies of the relative combining capacities of different antisera to Vibrio cholerae and Shigella flexneri were carried out using the Talmage test. In this test the absorption of an I(131) labelled antibody preparation by antigen is blocked by the addition of unlabelled unknown serum. Sera from rabbits in the early stages of immunization ("early antisera") had a high agglutinin titer, but low combining capacity. Sera from hyperimmune rabbits ("late antisera") had higher combining capacities than early antisera, but similar or lower agglutinin titers. More antigen was needed to absorb the agglutinins from late antisera than from early ones of the same agglutinin titer, indicating that late antisera contained relatively more antibody. At high concentrations, sera from hyperimmune rabbits agglutinated the homologous antigen more rapidly than did early antisera, even though early sera had similar or higher agglutinin titers. Sera drawn after the anamnestic response to bacterial antigen had the characteristics of late sera; i.e., their combining capacities were high. The possible significance of these findings for the in vitro estimation of protective antibody is discussed.

Animals↗
PubMed1957

Use of different I 131-labelled antibody eluates in the characterization of Shigella and Vibrio antisera.

The results presented indicate that blocking curves of decreased slope are obtained with the Talmage test when the labelled antibody eluate contains a portion of antibody which is not represented in the unknown serum. The blocking curves of late antisera had steeper slopes than those of early antisera when tested against labelled antibody eluates prepared from late antisera. This situation was reversed when labelled eluates from early antisera were used in the Talmage test. The findings were interpreted to indicate that early antisera did not have a full complement of antibodies, directed against all surface antigens of the bacterial cell.

Antibodies↗