The physical environment in soil microbiology: an attempt to extend principles of microbiology to soil microoganisms.
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The reduced availability of water resources in semi-arid Mediterranean regions requires an efficient use of supply sources. Urban wastewater, after treatment to minimise health hazards, may constitute an important resource for irrigation in areas characterised by intensive agriculture. These considerations have motivated an investigation (during the irrigation season 1996) of the dynamics of microbial biomass in the soil of a citrus orchard in eastern Sicily, which has been irrigated for 15 years with lagooned urban wastewater, to evaluate the effects of this practice on soil fertility. The analyses of parameters regarding soil microbial biomass (microbial carbon and microbial nitrogen, soluble carbon and nitrogen, cumulative respiration, respiratory quotient and enzymatic activity in the soil) have confirmed that the evolution of soil microflora is directly conditioned by the type of water used for irrigation and climatic conditions. Just before the beginning of the irrigation season (May), microbial biomass carbon (MBC), soluble C, cumulative respiration and enzymatic activity were significantly higher in the soil irrigated with wastewater with respect to the same soil irrigated with 'clear' water; the qCO2 was significantly lower. In June, after 1 month of irrigation, both soils showed an increase of all microbial parameters except for enzymatic activity and qCO2 which showed a significant reduction. In September, at the end of the irrigation period, there was a decrease of almost all investigated parameters in both plots, which was more evident in the soil irrigated with 'clear' water. The microbial biomass of the soil irrigated with wastewater, during the irrigation period, did not undergo any negative effects, having an evolution analogous to the plot irrigated with 'clear' water. The use of lagooned wastewater after three lustrums has shown, particularly in the dry season, an increase in quantity of easily available nutrients, with an improvement of the metabolic efficiency of soil microflora coupled with a more marked activity of total hydrolase and phosphatases. The variations of the parameters related to the soil microflora were strongly influenced by the seasonal climatic trend.
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Soil dilutions were studied by different techniques: They were retained on cellulose filters and observed either directly by scanning electron microscopy or after inclusion and ultra-thin sectioning by transmission electron microscopy. Morphology and locomotor organs of bacteria were observed after negative staining. They were plated on various culture media in order to isolate and identify most of the nonexacting microorganisms. Results are compared and the use of electron microscopy in soil studies is discussed.
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A historical essay of creative path of collective of the Department of General and Soil Microbiology, founded in 1928, is presented. Two research schools have been formed for the past years: ecophysiology of soil microflora and geochemical activity of microorganisms. L. I. Rubenchik stood at the sources of these schools. A problem of soil biology and microorganisms interrelations with higher plants occupies a considerable place in the Department research. Separate groups, microorganisms and microbe cenoses are studied as a whole as the complex self-regulated system. The investigations of microbe corrosion of metals initiated by a necessity to solve urgent problems of Kyiv metro have been formed as the priority trend. Agents and mechanisms of biological damages of the overground, underground and underwater structures are studied and the grounds of anticorrosion measures are developed within the framework of these trends.
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